-
No products found
because this supplier's products are not listed.
Liisa Nissinen, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... HEK and HOK (human oral keratinocytes) were from ScienCell Research Laboratories (Carlsbad ...
-
No products found
because this supplier's products are not listed.
Cary R. Boyd-Shiwarski, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
-
No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Juan A. Ortega, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Image acquisition for HEK-293 experiments was performed on a Leica DMI4000B laser scanning confocal microscope (Leica, Buffalo Grove, IL) or with Leica DMi8 microscope (Leica ...
-
No products found
because this supplier's products are not listed.
Maike Pohlers, et al.,
bioRxiv - Cancer Biology 2023
Quote:
For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
-
No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Dilutions of human serum (Human Serum age 4-6, Innovative Research) were performed in TBSM ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Astrid Hendriks, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4°C) and incubated with goat anti-human IgG-Alexa Fluor 647 and goat anti-human IgM-PE (both Southern Biotech) for 30 minutes at 4°C at 600 rpm ...
-
No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
No products found
because this supplier's products are not listed.
Michelle E. Gray, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and incubated overnight at 4°C with goat anti-human IgG (1:200; Jackson ImmunoResearch Laboratories ...
-
No products found
because this supplier's products are not listed.
Isabel Koh, Masaya Hagiwara,
bioRxiv - Bioengineering 2022
Quote:
Human iPSCs (IMR90-4; WiCell Research Institute) were maintained with mTeSR Plus (STEMCELL Technologies, 100-0276) on dishes coated with 9-10 μg/cm2 Growth Factor Reduced Matrigel (Corning ...
-
No products found
because this supplier's products are not listed.
Raphael Fettig, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... NIH-3T3 fibroblasts and HEK-293 cells (all from ATCC, LGC Standards GmbH, Wesel, Germany) were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
Cat# LS004191,
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Masaru Ito, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... TIM) containing 2 mg of collagenase (Worthington, LS004186) for incubation at 32°C for 55 min with shaking at 53 rpm ...
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No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Masatoshi Ohgushi, Mototsugu Eiraku,
bioRxiv - Developmental Biology 2021
Quote:
... ELISA kits were purchased from Abcam (human BMP-4 and CGβ) and Proteintech (human GDF15) ...
-
No products found
because this supplier's products are not listed.
Maxime Lorenzini, et al.,
bioRxiv - Physiology 2020
Quote:
Whole-cell NaV currents were recorded at room temperature from transiently transfected HEK-293 cells using an Axopatch 200A amplifier (Axon Instruments, Molecular Devices, San Jose, CA) 48 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Erin A. Stephens, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... 4 μM human UbcH5α/UBE2D1 (Boston Biochem), 3 μM uAb (or equivalent control protein) ...
-
No products found
because this supplier's products are not listed.
Shuaijie Dou, et al.,
bioRxiv - Immunology 2021
Quote:
... and the antibody to Tim-3 (A2516) for western Blot was obtained from Abclonal.
-
No products found
because this supplier's products are not listed.
Eri Takematsu, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Viruses were produced in HEK-293Ta cells using human lentiviral packaging system according to the manufacturer’s instructions (Genecopoeia). Puromycin was used to select only for transduced cells ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Robert Bucki, et al.,
bioRxiv - Biophysics 2022
Quote:
... Recombinant human protein arginine deiminase 4 (PAD4) was from Cayman Chemical, Inc.
-
No products found
because this supplier's products are not listed.
Theanne N. Griffith, et al.,
bioRxiv - Neuroscience 2019
Quote:
... TdTomato-expressing (Vglut3lineage) dissociated DRG neurons and HEK cells were performed with patch pipettes pulled from standard borosilicate glass (1B150F-4, World Precision Instruments) with a P-97 puller (Sutter Instruments) ...
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No products found
because this supplier's products are not listed.
Maik Wolfram-Schauerte, et al.,
bioRxiv - Microbiology 2023
Quote:
... 4 or 0.95 μM ARH1 or ARH3 (human, recombinant, Enzo Life Science) 5 in the presence of 10 mM Mg(OAc)2 ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Jing-Hui Tian, et al.,
bioRxiv - Microbiology 2020
Quote:
... Baboon IFN-γ and IL-4 assays were carried out using NHP IFN-γ and Human IL-4 assay kit from Mabtech using PBMC collected at day 7 following the second immunization (day 28).
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Takanori Matsubara, et al.,
bioRxiv - Neuroscience 2020
Quote:
Whole-cell patch-clamp recordings from cultured HEK 293 cells or VTA-DA neurons in acute brain slices were performed using an IPA amplifier (Sutter Instruments) at RT ...
-
No products found
because this supplier's products are not listed.
Shang Geng, Xing Lv, Weiwei Zheng, Tianjun Xu,
bioRxiv - Immunology 2024
Quote:
... The lysate was incubated over night at 4°C with anti-His beads (Solarbio). Anti-His beads were washed subsequently with washing buffer (500 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Purified human complement proteins (4 µg/mL, Complement Technology) were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL ...
-
No products found
Tamara Miljuš, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
For all experiments human embryonic kidney (HEK) 293SL cells84 were used and regularly tested for mycoplasma contamination (PCR Mycoplasma Detection kit, ABM, Canada). HEK293SL cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Pablo Rougerie, Dianne Cox,
bioRxiv - Cell Biology 2020
Quote:
... The gel was then immediately rinsed with distilled water and incubated overnight at 4°C with a 1mg/mL solution of human whole IgG (CellSciences) or human Fab fragment (Rockland) in 50mM HEPES pH 8 ...
-
No products found
because this supplier's products are not listed.
Kevin R. Bewley, et al.,
bioRxiv - Pathology 2020
Quote:
... were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories) at 5 μg/mL in PBS ...
-
No products found
because this supplier's products are not listed.
Yide Zhang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Human hairs were embedded in a 4% agarose block (A-204-25, GoldBio), and the block was placed in the water-filled customized container during imaging ...
-
No products found
because this supplier's products are not listed.
Yu Rebecca Miao, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and mouse anti-HIS Hilyte Fluor 488 (Anaspec).
-
No products found
because this supplier's products are not listed.
Xinrui Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His-tone H2A.Z (1:200, Active Motif #39013), Nkx2.5 (1:250 ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
No products found
because this supplier's products are not listed.
Gabriel Brawerman, et al.,
bioRxiv - Cell Biology 2021
Quote:
... aliquots of the low and high glucose supernatants and the insulin content extracts were collected and spun at 3000g for 5 minutes at 4°C and stored at −20°C or used immediately for mouse or human insulin ELISAs (Mercodia) with appropriate dilutions (1:10-1:50 for supernatants ...
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
-
No products found
because this supplier's products are not listed.
Jelke J. Fros, et al.,
bioRxiv - Microbiology 2021
Quote:
... Human blood (Sanquin Blood Supply Foundation ...
-
No products found
because this supplier's products are not listed.
Xuanwen Li, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ...
-
No products found
because this supplier's products are not listed.
Sunny Xia, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Human intestinal organoid swelling as tracked for 4 hours and imaged at 15 mins intervals using confocal microscopy (Nikon A1R Confocal Laser Microscope). Organoid swelling analysis was performed using Cell Profiler v3.1.
-
No products found
because this supplier's products are not listed.
Redouane Aherrahrou, et al.,
bioRxiv - Genetics 2023
Quote:
... Human aortic SMCs (Cell Applications, Inc. ...
-
No products found
because this supplier's products are not listed.
Nadine Nagy, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
4-MU (Alfa Aesar) was pressed into the mouse chow by TestDiet® and irradiated before shipment ...