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No products found
because this supplier's products are not listed.
Zhongxiao Wang, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Primary antibodies: anti-SLC38A5 (Biorbyt, St. Louis, MO; orb317962,), anti-non-phosphorylated β-catenin (Cell Signaling Technology ...
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No products found
because this supplier's products are not listed.
Christoph Ruschil, et al.,
bioRxiv - Immunology 2020
Quote:
... we performed specific enzyme immunoassays to measure IgG-antibodies against tick borne encephalitis in human serum (Immunozym FSME IgG, REF 7701010, PROGEN, Heidelberg, Germany). The ELISA tests were performed according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Priyatham Gorjala, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... was from Millipore-Sigma (St. Louis, MO) and anti-GAPDH (10R-G109A) was from Fitzgerald Industries (Acton ...
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No products found
Tian Lan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... samples were placed in a CO2 supplementary system (In Vitro Scientific, St. Louis, MO), maintained at 10-% CO2 and 37 °C ...
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No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
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Native Antigen
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Subhasis Mahari, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Human Immunodeficiency Virus (HIV) and Avian Influenza Virus (AIV) Ag were obtained from The Native Antigen Company (Oxford, UK). Aurdino software has been used in the in-house built device and the hardware include a printed Circuit Board (PCB) ...
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No products found
because this supplier's products are not listed.
Haoran Jing, et al.,
bioRxiv - Bioengineering 2020
Quote:
... MgCl2 were purchased from Sigma-Aldrich (St. Louis, MO, USA). In vivo Mab OKT3 antibody (Cat# BE0001-2-25MG, Lo. 683618M2) was purchased from BioXCell (Lebanon, NH, USA). Poly(acrylic acid) ...
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No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus was diluted in RPMI-1640 (Genesee Scientific) media containing 2% FBS and 10 mM HEPES ...
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No products found
because this supplier's products are not listed.
Kelly E. Dunham, et al.,
bioRxiv - Neuroscience 2023
Quote:
... were purchased from Sigma Aldrich (St Louis, MO, USA).(Dunham & Venton, 2020, 2022) meta-Chlorophenylpiperazine (m-CPP, 65369-76-8) was purchased from Alfa Aesar (Haverhill, MA, USA). FD&C Blue No ...
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No products found
because this supplier's products are not listed.
Hannah A. Pizzato, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Finck (Washington University in St. Louis) and were generated as described (McCommis et al., 2015) (currently available from The Jackson Laboratory, stock number 032118). Cpt2fl/fl mice were kindly provided by Dr ...
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No products found
because this supplier's products are not listed.
Stuti Mehta, et al.,
bioRxiv - Molecular Biology 2022
Quote:
AAVPrime™ Adeno-associated virus (AAV) Serotype Testing Kit (GeneCopoeia) was used to determine the efficiency of 8 different AAV serotypes in infecting the HCC1187 cell line ...
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No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
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No products found
because this supplier's products are not listed.
Qingtao Sun, et al.,
bioRxiv - Neuroscience 2023
Quote:
... IL-6 levels were quantified in the lysates using a Mouse IL-6 ELISA specific for lysates (#ELM-IL6-CL-1; RayBiotech).
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No products found
because this supplier's products are not listed.
M. Bormann, et al.,
bioRxiv - Microbiology 2021
Quote:
... the infectious virus was recovered by vortexing the carriers placed in plastic containers (SARSTEDT, Nümbrecht, Germany) filled with 2 mL DMEM for 1 minute ...
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No products found
because this supplier's products are not listed.
José-María Díez, Carolina Romero, Rodrigo Gajardo,
bioRxiv - Immunology 2020
Quote:
... Human Anti-SARS-CoV-2 Virus Spike 1 [S1] IgG ELISA Kit (Alpha Diagnostic Intl. Inc.), against S1 subunit spike protein ...
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No products found
because this supplier's products are not listed.
Helene Sertznig, et al.,
bioRxiv - Microbiology 2021
Quote:
... The virus containing supernatant was then purified by filtration through 0.30 μm MACS SmartStrainers (Miltenyi Biotec), aliquoted and stored at -80 °C ...
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No products found
because this supplier's products are not listed.
Matthew T. Birnie, et al.,
bioRxiv - Neuroscience 2022
Quote:
Virus labeled sections were scanned under a 10x objective of a fluorescent microscope (Olympus BX 61) equipped with a high-sensitivity CCD camera (Nikon Eclipse E400) ...
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No products found
because this supplier's products are not listed.
He You, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The AssayMax Corticosterone ELISA Kit (Assaypro, St. Charles, MO) was used to measure corticosterone levels in the blood samples ...
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No products found
because this supplier's products are not listed.
Wren E. Michaels, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cell lysates were prepared using the sample preparation kit (Protein Simple) for an automated capillary western blot system ...
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No products found
because this supplier's products are not listed.
Guus Franken, et al.,
bioRxiv - Immunology 2023
Quote:
... Serially diluted lysates were arrayed on nitrocellulose-coated slides (Grace Bio-Labs) by the Quanterix (Aushon ...
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No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
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No products found
because this supplier's products are not listed.
Lakshmi E. Batachari, et al.,
bioRxiv - Immunology 2024
Quote:
... samples were incubated with Quasar 670-conjugated FISH probes that hybridize to Orsay virus RNA1 and RNA2 (Biosearch Technologies). Following a 6-8 h incubation at 47 °C ...
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No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
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No products found
because this supplier's products are not listed.
Michael Bauer, et al.,
bioRxiv - Microbiology 2020
Quote:
... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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No products found
because this supplier's products are not listed.
Xiaofeng Zheng, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Insulin and protein content of the lysate were measured using Mouse Insulin ELISA (Mercodia) and BCA assay (ThermoFisher Scientific) ...
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No products found
because this supplier's products are not listed.
Jennifer B. Glass, et al.,
bioRxiv - Microbiology 2021
Quote:
... 0.4 μL of Klentaq LA Taq Polymerase (DNA Polymerase Technology, St. Louis, MO, USA), 2 μL of 10 mM dNTP mix (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Flavia Chiuppesi, et al.,
bioRxiv - Immunology 2020
Quote:
... or vesicular stomatitis virus G (VSV-G, Aldevron), using FuGENE6 (Roche ...
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No products found
because this supplier's products are not listed.
Caire Robin, et al.,
bioRxiv - Cell Biology 2022
Quote:
... aureus load of cell lysates was quantified on an agar plate using an automatic plate seeder (EasySpiral Dilute, Interscience, St-Nom la Bretèche, France) and a colony counter (Scan 4000 ...
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No products found
because this supplier's products are not listed.
Margaret A. Myers, et al.,
bioRxiv - Systems Biology 2021
Quote:
... Laboratory Autoclavable Rodent Diet (PMI Nutrition International, St. Louis, MO; SJCRH) or Teklad LM-485 Mouse/Rat Sterilizable Diet (Envigo, Indianapolis, IN; UTHSC) and autoclaved water were available ad libitum ...
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No products found
because this supplier's products are not listed.
Emily D Hartjes, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... An indoxyl sulfate concentration-response effect on CYP3A4 mRNA expression was generated using a concentration range of indoxyl sulfate found in normal and CKD patients (0 to 1000 µM) with 40 g/L human serum albumin (HSA, Lee Biosolutions, St. Louis, MO). Indoxyl sulfate was incubated in media containing 40 g/L HSA for 3 hours at 37°C to allow for plasma protein binding equilibration prior to cell treatment ...
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No products found
because this supplier's products are not listed.
Aarthi Subramani, et al.,
bioRxiv - Immunology 2020
Quote:
... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St. Louis, MO, and Peprotech, Rocky Hill, NJ) to promote repolarization to the M2 state ...
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No products found
because this supplier's products are not listed.
Kristyn Hoffman, et al.,
bioRxiv - Microbiology 2020
Quote:
... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific, Waltham, MA; CTGF and PDGF-D: G-Biosciences, St. Louis, MO; Abclonal, Woburn, MA) as previously reported and the data from these prior experiments was used in the current investigations.22 Each experiment was performed with two technical replicates.
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No products found
because this supplier's products are not listed.
Christine Vazquez, Chin Yee Tan, Stacy M. Horner,
bioRxiv - Microbiology 2019
Quote:
... and rabbit anti-Sendai virus (SV) (1:1000, MBL International). Secondary antibody incubations were done with Alexa Fluor conjugated antibodies (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Šimon Borna, et al.,
bioRxiv - Immunology 2022
Quote:
... and AAV6 virus (Signagen laboratories, and in house produced (33))-mediated delivery of homologous template containing NGFR reporter gene under control of PGK promoter as described previously (33) ...
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No products found
because this supplier's products are not listed.
S. Jake Gonzales, et al.,
bioRxiv - Immunology 2021
Quote:
... coli lysate (MCLAB #ECCL-100) was resuspended in MQ water and added to a final concentration of 15 µg/ml ...
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No products found
because this supplier's products are not listed.
Jiayang Chen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Ctip2 or Brn2 incubated sections were reacted with appropriate biotinylated secondary antibody for 1 hour (B7139; Sigma-Aldrich, St. Louis, MO; 1:200 or BA-9200; Vector Labs, Burlingame, CA; 1:200 respectively). The sections were then reacted with an avidin-biotin conjugate (ABC kit ...
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No products found
because this supplier's products are not listed.
Robert C. Hurt, et al.,
bioRxiv - Bioengineering 2024
Quote:
... a lysate clearing plate from Bio Basic (SD5006), and a DNA-binding plate from Epoch Life Sciences (2020-001) ...
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No products found
because this supplier's products are not listed.
Nia Toshkova, et al.,
bioRxiv - Immunology 2023
Quote:
... measles virus mosaic hemagglutinin (viral proteins were obtained from Immune Technology, New York, NY). Plates were incubated with proteins usually diluted to 5 μg/ml in PBS ...
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No products found
Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
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No products found
because this supplier's products are not listed.
Pawel Sledzinski, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Lysates were digested with proteinase K (Norgen Biotek Corp.) and decrosslinked at 65 °C overnight ...
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No products found
because this supplier's products are not listed.
Bruno Vaz, et al.,
bioRxiv - Immunology 2020
Quote:
Human APCs were pre-pulsed for 4h at 37 °C with 1:50 antigen mix (HBV, influenza and CMV lysates; bulk telomere measurements) or 1:50 cytomegalovirus (CMV)-lysates (Zeptometrix Corporation; IF-FISH studies). APC-T cell conjugates were then formed by adding autologous CD27+ CD28+ CD3+ CD4+ T cells in a 3:1 ratio ...
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No products found
because this supplier's products are not listed.
Hong-Wen Tang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Lysate was incubated with GFP-Trap agarose beads (Bulldog Bio) or anti-Flag M2 magnetic beads (Sigma ...
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No products found
because this supplier's products are not listed.
Charul Jani, et al.,
bioRxiv - Microbiology 2023
Quote:
... Lysates were run on a 10% acrylamide gel (National Diagnostics) in 1X Tris-Glycine SDS Running Buffer (Boston BioProduct ...
-
No products found
because this supplier's products are not listed.
Andrea Ridolfi, et al.,
bioRxiv - Biophysics 2022
Quote:
... anti-HBB (1:1000 Abnova, Jhouzih St., Taipei, Taiwan). For hCPC-EV samples ...
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No products found
because this supplier's products are not listed.
Bradley E. Poulsen, et al.,
bioRxiv - Microbiology 2024
Quote:
... Lysate was incubated with 0.05% N-Dodecyl-β-D-maltoside (Anatrace) for 1 hour at 4°C with rocking and then spun down at 20,000g for 20 minutes at 4°C to remove cell debris ...
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No products found
because this supplier's products are not listed.
Ke-Ming Xie, et al.,
bioRxiv - Microbiology 2024
Quote:
Total nucleic acids were extracted using the HP Virus DNA/RNA Kit (R6873; Omega Bio-Tek, Norcross, USA), and carrier RNA was not used during the process to avoid potential interference with sequencing results ...
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No products found
because this supplier's products are not listed.
Shawn Freed Jr, Nancy D. Hanson,
bioRxiv - Microbiology 2023
Quote:
... Whole cell lysates were collected by bead-beating (Next Advance Bullet Blender) and protein concentrations determined using a Bicinchoninic Acid Assay (Pierce BCA Protein Assay ...
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No products found
because this supplier's products are not listed.
Thomas H Mahood, et al.,
bioRxiv - Systems Biology 2020
Quote:
... CA) while tissue lysate samples were loaded onto a separate C18 column (Phenomenex) offline ...
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No products found
because this supplier's products are not listed.
Wei Qin, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The lysates were reacted with 100 μM azide-PEG3-biotin (Click Chemistry Tools), 500 μM CuSO4 ...
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No products found
because this supplier's products are not listed.
Nicholas J. Gunn, et al.,
bioRxiv - Microbiology 2023
Quote:
... the lysate was treated with the photoreactive DNA-binding dye PMAxx (Biotium, CA, USA). Briefly ...