-
No products found
because this supplier's products are not listed.
Shuibang Wang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Apoptotic cells (AV+ / PI-) were gated and counted using MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec).
-
No products found
because this supplier's products are not listed.
Michael Notaras, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Reagents applied to synaptic channels were as follows: Cycloheximide (10 μM, Calbiochem), MG132 (10 μM ...
-
No products found
because this supplier's products are not listed.
Yi-Wen Chang, et al.,
bioRxiv - Systems Biology 2020
Quote:
... ATP synthase β subunit (GeneTex), HA-tag (BioLegend ...
-
No products found
because this supplier's products are not listed.
Vaishali Aggarwal, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and PMT voltages were adjusted to predetermined target channels using the seventh peak of 8-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL, Cat. No. RCP-30-5A;) as a reference point ...
-
No products found
because this supplier's products are not listed.
Xiaolu Wang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... slices were incubated in goat anti-cholera toxin B subunit primary antibody (1:15000, List labs, 703) over night at 4 °C and biotinylated horse anti-goat secondary antibody (1:2000 ...
-
No products found
because this supplier's products are not listed.
Pietro Mesirca, et al.,
bioRxiv - Cell Biology 2020
Quote:
... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
-
No products found
because this supplier's products are not listed.
Nikolay S. Ilyinsky, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The emission was measured in CLSM Channel-mode using a 2-channel detector (Carl Zeiss, Germany) set to a 488–523 nm (Channel 1 ...
-
No products found
because this supplier's products are not listed.
Carlo N. G. Giachello, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Whole-cell voltage- and current-clamp recordings were achieved using thick-walled borosilicate glass electrodes (GC100F-10, Harvard Apparatus, Edenbridge, UK) fire polished to resistances of 10-15 MΩ (L3 aCC ...
-
No products found
because this supplier's products are not listed.
Francie Moehring, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Keratinocytes were patch clamped in both voltage clamp and current clamp mode (holding voltage -40 mV) with a borosilicate glass pipette (Sutter Instrument Company, Novato, CA) filled with intracellular normal HEPES solution containing (in mM) ...
-
No products found
because this supplier's products are not listed.
Alena Maul, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recorded voltage signals were amplified (Neuroprobe 1600, A-M Systems), bandpass filtered (0.3-7 kHz) ...
-
No products found
because this supplier's products are not listed.
Claudia Dell’Amico, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Sections were then washed in D-PBS and treated with Sodium Citrate based-R-buffer A (EMS, #62706-10) in 2100-Retriever (EMS ...
-
No products found
because this supplier's products are not listed.
Jordan R. Barrett, et al.,
bioRxiv - Immunology 2023
Quote:
... A coupled lawn of goat anti-human IgG Fc (hFc; 50 µg/mL in 10 mM sodium acetate, pH 4.5) (Jackson ImmunoResearch) was then prepared before quenching with 0.5 M ethanolamine (pH 8.5 ...
-
No products found
because this supplier's products are not listed.
I. Deniz Derman, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Alpha Tubulin (TUBA4A) mouse monoclonal antibody (Origene) and MUC5AC monoclonal antibody (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Vargas Alexandra, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and alpha-tubulin (Active Motif, 39527). Horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibodies were from Invitrogen ...
-
No products found
because this supplier's products are not listed.
Ryan M. Jamiolkowski, et al.,
bioRxiv - Biophysics 2019
Quote:
... Fluorescence intensities in a Cy3 channel (550-620 nm) and a Cy5 channel (660-720 nm) were separated by a Dual View splitter (Photometrics) and recorded with an electron-multiplying charge-coupled device (EMCCD ...
-
No products found
because this supplier's products are not listed.
Sarah V. Paramore, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... The probes were for Mus musculus Fgf10 (channel 2, 446371) and Wnt5a (channel 2, 316791) and the fluorophores were Opal Polaris 520 and Opal 620 (Akoya Biosciences FP1487001KT and FP1495001KT) ...
-
No products found
because this supplier's products are not listed.
Paul B. Finn, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... Four images per well were acquired at 10x magnification in the green channel (200 ms exposure) and the red channel (600 ms exposure) every 2 to 3 hrs using an Incucyte (Sartorius). A cytotoxicity index was calculated by dividing total green fluorescence intensity per mm2 at every time point by the intensity per mm2 at the first time point ...
-
No products found
because this supplier's products are not listed.
Diana E. Mitchell, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 1:100 anti-BKbeta4 K+ channel (Antibodies Inc 75-086) and anti-GFP 1:500 (Rockland 600-101-215). Incubations were performed for 14h at 4° C followed by incubation with secondary antibodies ...
-
No products found
because this supplier's products are not listed.
Michael L. Beshiri, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
-
No products found
because this supplier's products are not listed.
Florian Gaertner, et al.,
bioRxiv - Immunology 2022
Quote:
... Interferon alpha was measured by ELISA (Mouse IFN Alpha All Subtype ELISA Kit, High Sensitivity, PBL Assay Science). Blood was left at room temperature for 20 min and after centrifugation serum was frozen at -20°C until further analysis ...
-
No products found
because this supplier's products are not listed.
Andrea A. Perreault, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 100uM alpha-thioglycerol (MP Biomedicals #155723). Terminal erythroid differentiation of purified proerythroblasts was induced by the addition of 0.4 U/ml human recombinant Epo (10kU/ml Epogen by Amgen ...
-
No products found
because this supplier's products are not listed.
Dylan M. McCormick, et al.,
bioRxiv - Microbiology 2021
Quote:
... coli DH5 Alpha Competent Cells (Zymo Research) per the manufacturer’s instructions and isolated using a QIAprep Spin Miniprep Kit (QIAGEN) ...
-
No products found
because this supplier's products are not listed.
Thai Le,
bioRxiv - Synthetic Biology 2021
Quote:
... 2021: Escherichia coli DH5 alpha (Takara (Korea)) ...
-
No products found
because this supplier's products are not listed.
Theofilus A. Tockary, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Alpha MEM with nucleosides (StemCell Technologies, Vancouver, Canada) were purchased for cell culture purposes ...
-
No products found
because this supplier's products are not listed.
Aaron R. Massey, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 2015) Mice transgenic for human alpha-synuclein and deficient for murine alpha-synuclein (TgSNCAWT;Snca-/-) were obtained from Jackson Laboratories (#10710) and crossed with mice heterozygous for murine alpha synuclein (Snca+/-) ...
-
No products found
because this supplier's products are not listed.
Shulin Li, et al.,
bioRxiv - Cell Biology 2021
Quote:
... operated at a voltage of 200 kV and K2 direct electron detector (Gatan, CA). Cellular organelles of interest were recorded in counting mode at a nominal magnification of 32,000 x ...
-
No products found
because this supplier's products are not listed.
Yu Du, et al.,
bioRxiv - Bioengineering 2019
Quote:
... then the channel was filled with mounting medium containing DAPI (Vector Laboratories). Images were acquired using a SCTR Leica confocal microscope and Leica application suite (LAS X ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Zane Kliesmete, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... Primary antibodies (chicken alpha-GFP, Aves Labs: GFP-1010 and rabbit alpha-Ki67 ...
-
No products found
because this supplier's products are not listed.
Álvaro González Cid, Ignacio Giménez, Neil Duncan,
bioRxiv - Physiology 2023
Quote:
Standard Argyrosomus regius gonadotropin subunit amino acidic sequences (alpha common, beta-FSH and beta-LH) were deduced from RNAseq (Illumina and Nanopore) analysis of mRNA extracted from meagre pituitaries ...
-
No products found
because this supplier's products are not listed.
Philipp Jung, et al.,
bioRxiv - Biophysics 2021
Quote:
... The following antibodies or reagents were used: anti-Integrin alpha-L (ITGAL) antibody (Antibodies-online), mouse anti-human CD28 antibody (BD Pharmingen) ...
-
No products found
because this supplier's products are not listed.
Pierluigi Di Chiaro, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... anti-Collagen IV alpha 2 (3 μg/ml, Atlas Antibodies, #HPA069337), anti-LAMA5 (5 μg/ml ...
-
No products found
because this supplier's products are not listed.
Julia Bär, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Spray voltage applied on a metal-coated PicoTip emitter (10 μm tip size, New Objective, Woburn, Massachusetts, US) was 1.7 kV ...
-
No products found
because this supplier's products are not listed.
Johann Aleith, et al.,
bioRxiv - Immunology 2022
Quote:
... 10 mM HEPES and 1 mM sodium pyruvate (PAN Biotech). After determination of vital cells using a hemocytometer and trypan blue (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Shannon K. Rashid, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Fluorescence signal was separated into green channel and red channel using a dichroic mirror (T565lpxr, Chroma Technology) and emission filters (green ...
-
No products found
because this supplier's products are not listed.
Adam Institoris, et al.,
bioRxiv - Neuroscience 2022
Quote:
... a voltage isolation unit and a concentric bipolar electrode (FHC). We softly positioned the electrode onto the surface of the slice by a micro-manipulator ∼250μm lateral to the penetrating arteriole ...
-
No products found
because this supplier's products are not listed.
Samat Amat, et al.,
bioRxiv - Microbiology 2021
Quote:
... Images were obtained using a JEOL JSM-7600F scanning electron microscope at an accelerating voltage of 2 kV or a JEOL JSM-6490LV scanning electron microscopy at an accelerating voltage of 15 kV (JEOL USA Inc., Peabody, MA, USA).
-
No products found
because this supplier's products are not listed.
Ben F. Brian IV, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Secondary incubations included horseradish-peroxidase-conju-gated rabbit anti-goat IgG (H+L) (Southern Biotech 6164-05), Streptavidin-HRP (Cell Signaling Tech #3999) ...
-
No products found
because this supplier's products are not listed.
Jessica Ciesla, Isreal Moreno Jr., Joshua Munger,
bioRxiv - Microbiology 2022
Quote:
... A 10 uL Neon™ pipette tip was used to extract 10 uL of the cell/gRNA/Cas9 solution which was then electroporated using the Neon™ Transfection System (voltage: 1650 V, width: 10 ms, pulses: 3) and transferred to a prepared 6-well dish (Greiner #82050-842) containing growth medium ...
-
No products found
because this supplier's products are not listed.
Maris-Johanna Tahk, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... from Bright-field and RFP channels (LED light source with excitation filter 531(40) nm and emission filter 593(40) nm for RFP channel (BioTek Instruments) with the following parameters for bright-field ...
-
No products found
because this supplier's products are not listed.
Kiwon Park, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... and sodium lauroyl sarcosinate in RSB buffer and were sonicated for 10 min (Diagenode Bioruptor). Extracts were then diluted (1:4 in RSB + T buffer ...
-
No products found
because this supplier's products are not listed.
Ben James, et al.,
bioRxiv - Neuroscience 2022
Quote:
... threshold relative to resting potential and ion channel conductances (Abbott, 1999 ...
-
No products found
because this supplier's products are not listed.
Li Wang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
-
No products found
because this supplier's products are not listed.
Yicheng Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... sodium dithionite (S0562, TCI), D-[2-3H]mannose (ART0120A ...
-
No products found
because this supplier's products are not listed.
Xiuran Yin, et al.,
bioRxiv - Microbiology 2019
Quote:
... or 1 mM unlabeled methanol and 10 mM13C-labeled sodium bicarbonate (13C-labeled substrates provided by Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Harihar Milaganur Mohan, et al.,
bioRxiv - Neuroscience 2021
Quote:
... were maintained in DMEM (HyClone™ DMEM/High Glucose +L-Glutamine, +Glucose, -Sodium Pyruvate, Cytiva Life Sciences) supplemented with 10% fetal bovine serum (Atlanta Biologicals) and 1X Penicillin/Streptomycin (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Linlin You, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... β subunit overexpression was induced by adding IPTG (Gold Biotechnology) to 1 mM and cell growth was monitored until apparent OD600 reached 0.9 ...
-
No products found
because this supplier's products are not listed.
Laurence Abrami, et al.,
bioRxiv - Cell Biology 2020
Quote:
... L-alpha-phosphatidylserine (Avanti polar lipids 840032C), and L-alpha-phosphatidylethanolamine (Avanti polar lipids 840026C ...
-
Cat# HY-15736-5 mg,
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Xiaohui Ju, et al.,
bioRxiv - Microbiology 2020
Quote:
... Monensin sodium (MedChemExpress, HY-N0150), Salinomycin (MedChemExpress ...
-
No products found
because this supplier's products are not listed.
Austin J. Graham, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
Sodium hyaluronate (72 kDa, Lifecore Biomedical), methacrylic anhydride (Sigma-Aldrich ...