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No products found
because this supplier's products are not listed.
Iva Ganeva, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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No products found
because this supplier's products are not listed.
Sophie-Marie Aicher, et al.,
bioRxiv - Microbiology 2021
Quote:
... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Eden Yifrach, et al.,
bioRxiv - Cell Biology 2021
Quote:
Fluorescein isothiocyanate (FITC) labeled peptides corresponding to the carboxyl-terminal 10 amino acids of Yhl045w (FITC-RKRVLGVAYL, Genscript) and Idp3 (FITC-YEDKKGMCKL ...
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No products found
because this supplier's products are not listed.
Federico Giannitti, et al.,
bioRxiv - Pathology 2022
Quote:
... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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No products found
because this supplier's products are not listed.
Viorica L. Lastun, et al.,
bioRxiv - Cell Biology 2021
Quote:
Embryonic fibroblasts were generated from RHBDL4 WT and RHBDL4 KO E14.5 embryos and immortalized using lentiviral transduction of SV40 virus large T antigen (Ef1a_Large T-antigen_Ires_Puro, Addgene plasmid 18922), as described by Christova et al ...
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No products found
because this supplier's products are not listed.
Fakhriedzwan Idris, et al.,
bioRxiv - Microbiology 2024
Quote:
... hepatitis delta virus (HDV) ribozyme and simian virus 40 (SV40) poly-A sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) at 50 °C for 60 min ...
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No products found
because this supplier's products are not listed.
Mainak Das Gupta, et al.,
bioRxiv - Biochemistry 2022
Quote:
... with the corresponding antigen tumor necrosis factor (TNFα; Abcam plc), as well as with the human FcR I (CD64 ...
-
No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... C-terminal peptides (Biomatik) were dissolved in 200 mM ammonium acetate (pH 6.9) ...
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No products found
because this supplier's products are not listed.
Jason Neidleman, et al.,
bioRxiv - Immunology 2020
Quote:
... or Influenza Virus Control Peptide Pool (Anaspec). As a positive control for cytokine detection ...
-
No products found
because this supplier's products are not listed.
Minghua Nie, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and SV40 large T antigen (LT) were introduced using the QuikChange Site-Directed Mutagenesis kit (Agilent Technologies) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Edward Armstrong, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... TYR or Mart-1/MLANA PepTivator peptide pools (15-mer peptide sequences with 11 amino acids overlap, Miltenyi Biotec) for 60 min at 37°C ...
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No products found
because this supplier's products are not listed.
Federico Giannitti, et al.,
bioRxiv - Pathology 2022
Quote:
... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
-
No products found
because this supplier's products are not listed.
Enrico Radaelli, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Tumor Antigen p53 (p53, Leica/Novocastra NCL-L-p53-CM5p ...
-
No products found
because this supplier's products are not listed.
Shawn A. Hallett, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... tetracycline-controlled transactivator (tTA) and the SV40 large T antigen polyadenylation signal (Takara Bio, Mountain View, CA), into B6SJLF1 fertilized eggs ...
-
No products found
because this supplier's products are not listed.
Jessica Brown, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Four μg of Tau12 (N-terminal [epitope = amino acids 6-18], BioLegend), HT7 (mid-region [epitope = amino acids 159-163] ...
-
No products found
because this supplier's products are not listed.
Elena Gómez-Abenza, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... the tumor cell suspensions were passed through a 40 µm filter (BD) into a clean 50 ml tube ...
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No products found
because this supplier's products are not listed.
Telmo A. Catarino, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... heat-mediated antigen retrieval was performed for 35-40 min with citrate-based antigen retrieving solution (cat. no. H-3300, Vector Laboratories), for cleaved-caspase 3 ...
-
No products found
because this supplier's products are not listed.
Martin Joseph Lett, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Screen for virus progeny production was done with SARS-CoV-2 antigen quick-test (Roche, 9901-NCOV-01G) (or CPE in E2T ...
-
No products found
because this supplier's products are not listed.
Yumi Itoh, et al.,
bioRxiv - Microbiology 2023
Quote:
... The relevant oligonucleotide of SV40 large T antigen NLS (PPKKKRKVED) was ligated into the pGEX2T vector (GE Healthcare) carrying the GFP gene at the C-terminus of the multi-cloning site to produce GST-SV40NLS-GFP protein (referred to as SV40 NLS) ...
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No products found
because this supplier's products are not listed.
Samantha K. Murphy, Michelle M. Arnold,
bioRxiv - Microbiology 2019
Quote:
... Simian MA104 cells were cultured in Medium 199 (M199; Corning) supplemented with 5% FBS ...
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No products found
because this supplier's products are not listed.
Michelle A. Baird, et al.,
bioRxiv - Cell Biology 2023
Quote:
... then large scale purified (Qiagen) for experimental use ...
-
No products found
because this supplier's products are not listed.
Michelle M. Bertke, et al.,
bioRxiv - Genomics 2019
Quote:
Gam1 carries a single (10 amino acid) N-terminal myc tag that was detected using c-myc antibody (sc-40, Santa Cruz Biotechnology) at 1:500 dilution ...
-
No products found
because this supplier's products are not listed.
Alan Rodriguez Carvajal, et al.,
bioRxiv - Biochemistry 2020
Quote:
... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
-
No products found
because this supplier's products are not listed.
Tsun Ki Jerrick To, et al.,
bioRxiv - Immunology 2022
Quote:
... Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen) at 37°C for 1.25 hours and then washed twice.
-
No products found
because this supplier's products are not listed.
Chuan Xu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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Native Antigen
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Subhasis Mahari, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Human Immunodeficiency Virus (HIV) and Avian Influenza Virus (AIV) Ag were obtained from The Native Antigen Company (Oxford, UK). Aurdino software has been used in the in-house built device and the hardware include a printed Circuit Board (PCB) ...
-
No products found
because this supplier's products are not listed.
Jashodeep Datta, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Tumor lysates were quantified and 40 μg protein loaded for analysis of GzB levels by ELISA (R&D Systems).
-
No products found
because this supplier's products are not listed.
Belinda J Hernandez, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and chicken anti-Sendai virus antigen (SeV, 1:5000, Charles River, 10100648).
-
No products found
because this supplier's products are not listed.
Bardees M. Foda, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Digested tumors were filtered using a 40 μm cell strainer (VWR# 352340), and the digestion reaction was stopped by adding 0.5 μM EDTA ...
-
No products found
because this supplier's products are not listed.
J.J. Patten, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus antigen was stained with SARS-CoV-2 specific antibody MM05 (Sino Biologicals, Beijing, China) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Tsukasa Oikawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
... HMLE cells were generated by introducing SV40 large T-antigen and human telomerase reverse transcriptase into a primary culture of normal mammary epithelial cells (Lonza). HMLE cells were cultured in MEGM ...
-
No products found
because this supplier's products are not listed.
Reza Nejat, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Z-RLRGG-7-amino-4-methyl-courmarin (peptide-AMC) was purchased from Bachem. Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC ...
-
No products found
because this supplier's products are not listed.
Kevin B. Weyant, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... Proteins and peptides bearing C-terminal 6xHis tags were detected with mouse anti-6xHis antibody clone AD1.1.10 (BioRad; Cat # MCA1396GA) while detection of glycosylated CRM197-FtO-PS was with anti-F ...
-
No products found
because this supplier's products are not listed.
Junzuo Liao, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... large tumor suppressor kinase 1 (LATS1) (1:300, Proteintech), phosphorylated (p)-YAP (1:1,000 ...
-
No products found
because this supplier's products are not listed.
Diego Cantoni, et al.,
bioRxiv - Microbiology 2020
Quote:
A 16 amino acids peptide (nh2-CMHRDYNHDMSDKHRA −conh2) was designed by Eurogentec based on the provide sequence of AOX of Naegleria gruberi (XP_002672918 ...
-
No products found
because this supplier's products are not listed.
Caleb Homiski, et al.,
bioRxiv - Microbiology 2023
Quote:
... Primary antibodies used were Ab101 (against large T-antigen) and Phospho-(Ser-Glu/Thr-Glu) ATM/ATR substrate antibody (pSQ/TQ) (Cell signaling Technology # 2851). Image J (NIH ...
-
No products found
because this supplier's products are not listed.
Camile C. Fontelles, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Antigen retrieval was performed by immersing the tissue sections at 98°C for 40 minutes in 1X Diva Decloaker (Biocare). Tissue sections were treated with 3% hydrogen peroxide and 10% normal goat serum for 10 minutes and were incubated with the primary antibody ...
-
No products found
because this supplier's products are not listed.
Candler Paige, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and 40 µM D(−)-2-amino-5-phosphonovaleric acid (APV; Tocris) and 50 nM of rat CGRP or vehicle ...
-
No products found
because this supplier's products are not listed.
Xiangyu Zhou, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin (Suc-LLVY-MCA; Peptide Institute, Cat#3120-v) in 100 mM Tris-HCl (pH 8.0 ...
-
No products found
because this supplier's products are not listed.
Anya Bauer, et al.,
bioRxiv - Microbiology 2022
Quote:
... The virion content of viral stocks was quantified using the Simian Immunodeficiency Virus (SIV) p27 Antigen Elisa kit from Zeptometrix (# 0801169) following the manufacturer’s recommendations.
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No products found
because this supplier's products are not listed.
Georgij P. Arapidi, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The mixture of peptides was separated from large proteins by centrifugation on Vivaspin 500 concentrators (Sartorius, Germany) with a PES membrane resistant to high concentrations of salts and detergents (10 kDa molecular weight cut-off) ...
-
No products found
because this supplier's products are not listed.
Giulio Matteucci, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Image acquisition and stitching of a large field of 7×7 mm with 40% overlap was handled by Nikon NIS-Elements AR 4.0 software ...
-
No products found
because this supplier's products are not listed.
BI Reinfeld, et al.,
bioRxiv - Cancer Biology 2020
Quote:
PyMT GEMM mice were bred by crossing male transgenic mice expressing the polyoma virus middle T antigen (PyMT) oncoprotein under the MMTV-LTR (Jackson Laboratory 022974) with wildtype females on a similar B6/FVB mixed background ...
-
No products found
because this supplier's products are not listed.
Yuan Gu, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... The percentages of Ki67-positive proliferating and cleaved casp-3-positive apoptotic tumor cells were quantified in 12 ROIs of each section at 40× magnification with a BX-60 microscope (Olympus).
-
No products found
because this supplier's products are not listed.
Sahar Shahamatdar, et al.,
bioRxiv - Pathology 2022
Quote:
... TruSight Tumor 15 (Illumina, USA), and in-house pyrosequencing [43] ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Yuko Hasegawa, Jason D. Lieb, Kevin Struhl,
bioRxiv - Molecular Biology 2019
Quote:
... Large fragments were removed using 0.4 × AMPure beads (Beckman Coulter). Libraries were sequenced on NextSeq 500.
-
No products found
because this supplier's products are not listed.
Stijn De Munter, et al.,
bioRxiv - Immunology 2023
Quote:
... and purified using Zymoclean Large Fragment DNA Recovery Kit (Zymo) according to manufacture instructions ...
-
No products found
because this supplier's products are not listed.
Paul B. Finn, et al.,
bioRxiv - Biochemistry 2020
Quote:
... (R)-2,4-Fmoc-Dab(Boc)-OH (α-amino-GABA turn) was purchased from Peptides International. Monomers were synthesized as previously described [32] ...
-
No products found
because this supplier's products are not listed.
Yunlong Cao, et al.,
bioRxiv - Immunology 2022
Quote:
... G*ΔG-VSV virus (VSV G pseudotyped virus, Kerafast) is used to infect 293T cells (American Type Culture Collection [ATCC] ...