-
No products found
because this supplier's products are not listed.
Ronja Wieboldt, et al.,
bioRxiv - Immunology 2023
Quote:
... Siglec-9 blocking antibody (Clone 191240, R&D Systems) was added at a final concentration of 10 µg/mL ...
-
No products found
because this supplier's products are not listed.
Alexandra Tsirigotaki, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
-
No products found
because this supplier's products are not listed.
Clarissa M Koch, et al.,
bioRxiv - Immunology 2022
Quote:
... Siglec F (BD), Ly6C (eBioscience) ...
-
No products found
because this supplier's products are not listed.
Filip Mihalic, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
-
No products found
because this supplier's products are not listed.
Dmitri I. Kotov, et al.,
bioRxiv - Immunology 2022
Quote:
... Siglec F (S17007L, BioLegend), CSF1R (AFS98 ...
-
No products found
because this supplier's products are not listed.
Yunlong Zhao, et al.,
bioRxiv - Immunology 2019
Quote:
... 3 nM mouse PD-L1-His/9 nM mouse CD80-His (Sino Biological, catalog 50446-M08H) combined ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Alexander Kuzin, et al.,
bioRxiv - Genomics 2021
Quote:
... human Hek293 DNA was purchased from Genscript. S ...
-
No products found
because this supplier's products are not listed.
Rosemary A. Bamford, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 1x penicillin/streptomycin (pen/strep; PAA) and distributed in 6-well plates with HEK293 cells transfected with Neo1.a-AP-His (Addgene #71963) or pcDNA3.1-CNTN4-FLAG ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Rebecca Garnham, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The following primary antibodies were used: anti-Siglec-9 (Proteintech,13377-1-AP, 1:200) anti-Siglec-7 (Proteintech ...
-
No products found
because this supplier's products are not listed.
Weihua Qin, et al.,
bioRxiv - Cell Biology 2022
Quote:
... His- tagged human UHRF1 was purified using Ni-NTA sepharose resin (Qiagen). Recombinant E1 (His-UBE1) ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
No products found
because this supplier's products are not listed.
Nicole Marquardt, et al.,
bioRxiv - Immunology 2020
Quote:
... Siglec-7 (5-386, Alexa Fluor 488, Bio-Rad), TIM-3 (7D3 ...
-
No products found
because this supplier's products are not listed.
Katarzyna Z Haza, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 5% CO2 for 24 h before transfection with a 4:1 DNA ratio of pCMV6-Affimer-tGFP and FLAG-ERK plasmids using Lipofectamine 2000 (SW620, HEK293, NCI-H460) or X-tremeGENE 9 (Roche; Panc10.05). After 24 h cells were serum starved for 1 h ...
-
No products found
because this supplier's products are not listed.
Kazuhiro Yamamoto, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human HMGB2 (C-terminal His tag, TP720732) from ORIGENE (Rockville, MD); anti-rabbit alkaline phosphatase-linked antibody ...
-
No products found
because this supplier's products are not listed.
Zachary R. Crook, et al.,
bioRxiv - Bioengineering 2024
Quote:
... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
-
No products found
because this supplier's products are not listed.
Tzu-Hsuan Chang, et al.,
bioRxiv - Immunology 2023
Quote:
Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
-
No products found
because this supplier's products are not listed.
Nilabhra Mitra, Sanghamitra Dey,
bioRxiv - Plant Biology 2021
Quote:
Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
-
No products found
because this supplier's products are not listed.
Maeva Dupont, et al.,
bioRxiv - Cell Biology 2019
Quote:
... anti-Siglec-1 (Novus Biologicals) and anti-pSTAT1 (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Thiago J. Borges, et al.,
bioRxiv - Immunology 2023
Quote:
... and mouse IgM anti-mouse Siglec-E (clone F-7, Santa Cruz) were used as 1:200 ...
-
No products found
because this supplier's products are not listed.
Chloe G Myers, et al.,
bioRxiv - Cell Biology 2024
Quote:
Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Bashir A. Ali, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or anti-His (Cell Signaling #2366 His-Tag 27E8) primary antibodies ...
-
No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
-
No products found
because this supplier's products are not listed.
Matthew Wortham, et al.,
bioRxiv - Physiology 2024
Quote:
Overnight recovered mouse or human islets were cultured with 0.1% DMSO (vehicle) or 9 µM AGK2 (Tocris), 25 µM AK-1 (EMD-Millipore) ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Pengfei Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
No products found
because this supplier's products are not listed.
Sam C. Nalle, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Siglec fusion proteins and control human IgG1 were assessed for glycan binding on the Glycan-300 (RayBiotech). The staining and analysis were performed as a service by RayBiotech using the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Takushi Shimomura, et al.,
bioRxiv - Biophysics 2019
Quote:
... and pClamp 9 software (Molecular Devices). The pipette solution contained 130 mM KCl ...
-
No products found
because this supplier's products are not listed.
Nicholas J. Silva, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
John P Stone, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... Human sC5b-9 (Complement Technology) at concentration ranging from 5 μg/mL to 0.01 μg/mL was used to generate a standard curve ...
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
Laura Pasetto, et al.,
bioRxiv - Neuroscience 2021
Quote:
The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
-
No products found
because this supplier's products are not listed.
Shuai Yan, et al.,
bioRxiv - Physiology 2023
Quote:
... 9-PAHSA (20µM) and 9-OAHSA (20µM) (Cayman Chemical), or Vehicle (DMSO ...
-
No products found
because this supplier's products are not listed.
Sara Cavallaro, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
-
No products found
because this supplier's products are not listed.
Matteo Guardamagna, et al.,
bioRxiv - Neuroscience 2021
Quote:
9 male C57BL6/J mice (Charles River) were used in this study ...
-
No products found
because this supplier's products are not listed.
Stephania Assimopoulos, et al.,
bioRxiv - Neuroscience 2021
Quote:
... and Shank3 (Δexon 4-9) (Jackson Laboratory #017890) (45 ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Jinwook Choi, et al.,
bioRxiv - Cell Biology 2020
Quote:
Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
-
No products found
because this supplier's products are not listed.
Lindsey A. Smith, et al.,
bioRxiv - Neuroscience 2021
Quote:
... TgF344-AD males harboring the amyloid precursor protein Swedish (APPswe) and delta exon 9 mutant human presenilin-1 (PS1∆E9) transgenes were bred to wildtype (Wt) F344 females (Envigo, Indianapolis, IN), as done previously in our lab (Smith and McMahon ...