-
No products found
because this supplier's products are not listed.
Yasuaki Anami, et al.,
bioRxiv - Bioengineering 2021
Quote:
... free style HEK-293 human embryonic kidney cells (Invitrogen) were transfected with a mammalian expression vector encoding for the human IgG1 kappa light chain and full-length heavy chain sequences (based on variable sequences of cetuximab ...
-
No products found
because this supplier's products are not listed.
Uriel López-Sánchez, et al.,
bioRxiv - Biochemistry 2024
Quote:
Human embryonic kidney 293 (HEK-293) cells (American Type Culture Collection, Manassas, VA) were cultured in growth medium (DMEM supplemented with 10% v/v fetal bovine serum ...
-
No products found
because this supplier's products are not listed.
Amy J Tibbo, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human embryonic kidney (HEK) 293 cells were purchased from ATCC and HEK-293 PDE4A4 stable cell line established by Millipore. HEK-293 and HEK-293 PDE4A4 cells were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Nazmul Haque, et al.,
bioRxiv - Molecular Biology 2022
Quote:
HEK-293 Tet-Off (HEK-293TO) cells (Clontech) and HEK-293 Flp-In T-REx cells (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jeffrey S. Smith, et al.,
bioRxiv - Cell Biology 2019
Quote:
... ‘ΔGsix’ HEK 293 cells were transfected using Fugene 6 (Promega) according to manufacturer specifications.
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No products found
because this supplier's products are not listed.
Prashanth Thevkar-Nagesh, et al.,
bioRxiv - Molecular Biology 2020
Quote:
Human embryonic kidney (HEK) 293 cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Corning) containing 10% fetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
Anani K Afanou, et al.,
bioRxiv - Microbiology 2022
Quote:
Human embryonic kidney (HEK) 293 reporter cells for TLR2 and TLR4 (Invivogen, France) were exposed to the eluted dust samples to study their potential to induce NFkB responses ...
-
No products found
because this supplier's products are not listed.
Aleksandra Vuchkovska, et al.,
bioRxiv - Immunology 2022
Quote:
... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
-
No products found
because this supplier's products are not listed.
Philip Schmassmann, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... live/dead stained and incubated with recombinant human Siglec-9 Fc chimera (#1139-SL-050) or recombinant mouse Siglec-E Fc chimera (#5806-SL-050) both from R&D systems, USA for 20 min on ice ...
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No products found
because this supplier's products are not listed.
Clarissa M Koch, et al.,
bioRxiv - Immunology 2022
Quote:
... Siglec F (BD), Ly6C (eBioscience) ...
-
No products found
because this supplier's products are not listed.
T. Ono, et al.,
bioRxiv - Bioengineering 2020
Quote:
... recombinant from human embryonic kidney (HEK) 293 cells (Sino Biological Inc., China) solution in phosphate buffer solution (10 mM ...
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No products found
because this supplier's products are not listed.
John R Sinnamon, et al.,
bioRxiv - Neuroscience 2020
Quote:
AAV vectors used in the study were produced in human embryonic kidney HEK 293 cells (AAV-293, Agilent, RRID: CVCL_6871) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride (CsCl ...
-
No products found
because this supplier's products are not listed.
Timothy M. Sveeggen, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Human embryonic kidney 293 cells (HEK 293FT) obtained from Lonza were cultured on flasks coated with 20 μg/ml collagen type I ...
-
No products found
because this supplier's products are not listed.
Megi Rexhepaj, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Anti-human Fc (Jackson ImmunoResearch) was diluted 1:50,000 in Intercept (TBS ...
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No products found
because this supplier's products are not listed.
Kinneret Shefer, et al.,
bioRxiv - Genomics 2020
Quote:
... Dharmacon) into HEK 293 cells with TransIT-X2 (mc-MIR-6003, Mirus) according to the manufacturer’s instructions with some modifications ...
-
No products found
because this supplier's products are not listed.
Sophie D. Adams, et al.,
bioRxiv - Cell Biology 2020
Quote:
... lentivirus was produced by transfecting HEK-293 cells with LV-RFP (Addgene 26001), pMD2.G (Addgene ...
-
No products found
because this supplier's products are not listed.
Sarah E. Brnich, et al.,
bioRxiv - Genetics 2020
Quote:
... and FCS Express 6 (De Novo Software) software.
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No products found
because this supplier's products are not listed.
Pablo Ranea-Robles, et al.,
bioRxiv - Physiology 2021
Quote:
... HEK-293 cells were cultured and treated with 2 µg/mL of tunicamycin (ab120296, Abcam) for 6 hours ...
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No products found
because this supplier's products are not listed.
Wiebke Ehrlich, Frank J.P. Kühn,
bioRxiv - Cell Biology 2023
Quote:
... Transfection of HEK-293 cells was performed with FuGene 6 transfection reagent (Roche, Mannheim, Germany) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Kari Salokas, et al.,
bioRxiv - Systems Biology 2021
Quote:
HEK 293 cells were cultivated in DMEM (GE Healthcare), supplemented with 10% foetal bovine serum (FBS ...
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No products found
because this supplier's products are not listed.
Vera Vorstandlechner, et al.,
bioRxiv - Cell Biology 2020
Quote:
... FBs were then stimulated with 10ng/ml TGFβ1 (HEK-293-derived, Peprotech, Rocky Hill, NJ, USA), and with or without DPP4-inhibitor Sitagliptin (10μM ...
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No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... anti-human IgA Fc or anti-human IgM Fc (Bethyl Labs, Montgomery, TX) at 2μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Sarah H Needs, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK 293 cells plated in 12 well plates (Greiner) at a density of 80 000 cells per well were transfected with ddVENUS plasmid DNA (2 μg per well ...
-
No products found
because this supplier's products are not listed.
Evan R. Semenza, et al.,
bioRxiv - Neuroscience 2021
Quote:
HEK 293 cells were transfected using PolyFect Transfection Reagent (Qiagen) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Nour Zeaiter, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or 10 μg human ACSS2-overexpressing HEK-293 cell lysate (ref. LY412981, Origene, MD ...
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No products found
because this supplier's products are not listed.
Sierra D. Kauer, et al.,
bioRxiv - Neuroscience 2021
Quote:
... HEK 293 cells were cultured in ten 15 cm plates and polyethylenimine (Polysciences) transfected with an AAV plasmid overexpressing a candidate gene ...
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No products found
because this supplier's products are not listed.
Inês Caldeira Brás, et al.,
bioRxiv - Neuroscience 2021
Quote:
... HEK 293 cells were seeded in culture plates (Corning, Merck, Darmstadt, Germany) and incubated in DMEM (PAN Biotech ...
-
No products found
because this supplier's products are not listed.
Duško Lainšček, et al.,
bioRxiv - Immunology 2020
Quote:
Recombinant human ACE2-Fc (Genscript) at concentration of 2 μg/ml in phosphate buffer saline (PBS ...
-
No products found
because this supplier's products are not listed.
Inês Caldeira Brás, et al.,
bioRxiv - Neuroscience 2021
Quote:
Human embryonic kidney (HEK) 293 cells (ATTC, VA, USA) were maintained in DMEM medium (PAN Biotech, Aidenbach, Germany) supplemented with 10% FBS (Anprotec ...
-
No products found
because this supplier's products are not listed.
Judith F. Ashouri, et al.,
bioRxiv - Immunology 2019
Quote:
... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively.
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No products found
because this supplier's products are not listed.
Wei-Li Ling, Samuel Ken-En Gan,
bioRxiv - Immunology 2022
Quote:
KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
No products found
because this supplier's products are not listed.
Chao Gao, et al.,
bioRxiv - Biochemistry 2020
Quote:
... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
-
No products found
because this supplier's products are not listed.
Audrey Cochard, et al.,
bioRxiv - Cell Biology 2021
Quote:
... HeLa cells and HEK-293 cells were cultured on 22×22mm glass coverslips (VWR) in 6-well plates (Falcon) ...
-
No products found
because this supplier's products are not listed.
Jinwook Choi, et al.,
bioRxiv - Cell Biology 2020
Quote:
Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
-
No products found
because this supplier's products are not listed.
Carolina Franco Nitta, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Human embryonic kidney HEK-293 cells were cultured in Minimum Essential Medium (MEM) with 10% fetal bovine serum (FBS; Atlanta Biologicals), 2 mM L-glutamine ...
-
No products found
because this supplier's products are not listed.
Florian Koban, Michael Freissmuth,
bioRxiv - Neuroscience 2021
Quote:
HEK-293 cells in different culture formats were transfected with 3 target-specific siRNAs against human MAD2 (sc-35837, Santa Cruz Biotechnology) or siRNA against Luciferase (Dharmacon ...
-
No products found
because this supplier's products are not listed.
Christina Vrettou, et al.,
bioRxiv - Immunology 2022
Quote:
... and blotted with anti-human IgG Fc (Jackson labs). (E ...
-
No products found
because this supplier's products are not listed.
Ketty Kessler, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... at 6 pM with 15% PhiX control DNA v3 (#FC-110-3001, Illumina) and sequenced on a MiSeq System (Illumina).
-
No products found
because this supplier's products are not listed.
Shigenori Inagaki, et al.,
bioRxiv - Neuroscience 2020
Quote:
The luciferase assay with HEK 293 cells (293AAV cell, Cell Biolabs) was performed as described previously (Tsuboi et al. ...
-
No products found
because this supplier's products are not listed.
Alexander P. Clark, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human embryonic kidney cells 293 stably expressing human hyperpolarization-gated cyclic nucleotide-sensitive cation channel 1 (HEK-HCN1) were obtained from Charles River (CT6114). Cells were cultured and maintained according to the online protocol by Charles River ...
-
No products found
because this supplier's products are not listed.
Eduardo U. Anaya, et al.,
bioRxiv - Immunology 2020
Quote:
HEK-293 cells were plated at 25,000 cells in a 35 mm (MatTEK dishes) 48h prior to imaging ...
-
No products found
because this supplier's products are not listed.
Veronica Locher, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Giulia Ferri, et al.,
bioRxiv - Pathology 2022
Quote:
Human embryonic kidney (HEK 293T) cells (ATCC Promocell; CRL-11268) were cultured using Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (Biochrom) ...
-
No products found
because this supplier's products are not listed.
Juan A. Ortega, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Image acquisition for HEK-293 experiments was performed on a Leica DMI4000B laser scanning confocal microscope (Leica, Buffalo Grove, IL) or with Leica DMi8 microscope (Leica ...
-
No products found
because this supplier's products are not listed.
Marina Vortmeyer-Krause, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
-
No products found
because this supplier's products are not listed.
Nicole Marquardt, et al.,
bioRxiv - Immunology 2020
Quote:
... Siglec-7 (5-386, Alexa Fluor 488, Bio-Rad), TIM-3 (7D3 ...
-
No products found
because this supplier's products are not listed.
Rebecca Garnham, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-Siglec-7 (Proteintech, 13939-1-AP, 1:200), anti-CD14 (Proteintech ...
-
No products found
because this supplier's products are not listed.
Parishmita Sarma, et al.,
bioRxiv - Biochemistry 2024
Quote:
... cells (HEK-293) were plated in 8-well µ-slides (iBidi, Germany) and transfected using Lipofectamine 3000 (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Rogger P. Carmen-Orozco, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and HEK-293 cells were extracted using Monarch Total RNA Miniprep Kit (New England BioLabs, T2010S). To prepare RNA-Seq libraries ...