-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Fangzhou Lou, et al.,
bioRxiv - Immunology 2020
Quote:
... Normal Human Epidermal Keratinocytes (NHEKs; Lifeline Cell Technology, cat. FC-0007) stored in liquid nitrogen were defrosted and cultured in serum-free basal medium with growth factors (Lifeline Cell Technology ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Jakub Ziak, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 5 nM Sema3A of control Fc was applied distally and axons were photographed by Leica DMI6000 microscope every 10 minutes during 14 hours interval ...
-
No products found
because this supplier's products are not listed.
Mina Ogawa, et al.,
bioRxiv - Cell Biology 2021
Quote:
... for 1-5 min exposures on the Li-Cor Odyssey Fc (LI-COR Biosciences, Lincoln, NE). Relative expression level of CFTR proteins were quantified by densitometry of immunoblots ...
-
No products found
because this supplier's products are not listed.
Harman Malhi, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-Human Fc biosensors (ForteBio, catalog #18-0015) were submerged in wells of black 96-well microplates (Greiner, catalog #655209) containing 250 μL of kinetics buffer (PBS ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Flag-PFDN2 and Flag-VIM) and the bacterial expression vectors pReceiver-B11 (His-NSMCE2 and His-PFDN2) were purchased from Genecopoeia, Inc ...
-
No products found
because this supplier's products are not listed.
Sumona P. Dhara, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Reactions were quenched in heat inactivated fetal calf serum (HI-FCS; Gemini Bio-Products) in DMEM/F12 (Gibco ...
-
No products found
because this supplier's products are not listed.
Jinwook Choi, et al.,
bioRxiv - Cell Biology 2020
Quote:
Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
Salim Benlefki, et al.,
bioRxiv - Neuroscience 2022
Quote:
... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
-
No products found
because this supplier's products are not listed.
Arne Praznik, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
5 x 104 HEK293-T cells per well were seeded in an 8-well chamber slide (ibidi). At a confluence of 5070% cells were transfected with a mixture of DNA and PEI (8 μl PEI/1,000 ng DNA) ...
-
No products found
because this supplier's products are not listed.
Allen G. Sanderlin, et al.,
bioRxiv - Microbiology 2022
Quote:
... and precipitated culture supernatants (equivalent to 2 mL of culture supernatant prior to precipitation) were analyzed by western blot using rabbit anti-FLAG and HRP-conjugated mouse anti-His (ABclonal #AE028).
-
No products found
because this supplier's products are not listed.
Erik Procko,
bioRxiv - Biochemistry 2020
Quote:
Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
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Recombinant Human IL3RA (NP_002174.1) extracellular domain (Met 1-Arg 305), fused with the...
Cat# IL3RA-3248H,
20ug , USD $298
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Chang-Hoon Kim, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ...
-
No products found
because this supplier's products are not listed.
Siou-Luan He, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme) ...
-
No products found
because this supplier's products are not listed.
Hongwen Chen, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the complex was eluted with 5 CVs of 3×Flag peptide (0.1 mg/ml; ApexBio). The eluted protein was further purified by gel filtration using a Superose 6 Increase 10/300 GL column (Cytiva ...
-
No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 10% FCS (PromoCell, Germany). HEK-NF-κB_luc were supplemented for routine growth with 50 µg/mL Hygromycin B (Invivogen ...
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Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1...
Cat# B26102, SKU# B26102-5 mL,
5 mL, $999.00
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Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... Immunoprecipitation was performed with anti-His or Flag magnetic beads (Bimake, Texas, US), according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jordi Rodon, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 5% fetal calf serum (FCS; EuroClone), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Lupeng Ye, et al.,
bioRxiv - Bioengineering 2023
Quote:
... supplied with 5 % human AB serum (MP Biomedicals) and 400U/mL human IL-2 (BioLegend) ...
-
No products found
because this supplier's products are not listed.
Julianne B. Riggs, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
because this supplier's products are not listed.
Maurice Michel, et al.,
bioRxiv - Biochemistry 2024
Quote:
... goat anti-human IgG-Fc Alexa488 (Dianova, 1:400) or goat antihuman IgM Alexa 594 (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Djem U. Kissiov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... In all cases medium contained 5% FCS (Omega Scientific), 0.2 mg/mL glutamine (Sigma) ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Sharon Wei Ling Lee, et al.,
bioRxiv - Systems Biology 2020
Quote:
... supplemented with 5% human serum (Innovative Research, IPLA-SER) and 1% 1X Penicillin-Streptomycin (hereafter referred to as “cIMDM”) ...
-
No products found
because this supplier's products are not listed.
Kaitavjeet Chowdhary, et al.,
bioRxiv - Immunology 2023
Quote:
... Cells were sorted into DMEM + 5% FCS in DNA Lo-Bind tubes (Eppendorf, cat # 022431021). After spinning down for 5 min at 500g in a refrigerated centrifuge at 4°C ...
-
No products found
Kirandeep K. Deol, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 Expressing Rpn11-HTBH (Applied Biological Materials, T6007)
-
No products found
because this supplier's products are not listed.
Quinton Smith, Christopher Chen, Sangeeta Bhatia,
bioRxiv - Bioengineering 2021
Quote:
Human intrahepatic biliary epithelial cells (IHCs) passage 1-5 (ScienCell, Carlsbad, CA) were cultured in complete epithelial growth media (ScienCell ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
-
No products found
because this supplier's products are not listed.
Mukundan Attur, et al.,
bioRxiv - Cell Biology 2019
Quote:
... supplemented with 10% FCS (Atlanta Biologicals). Cells at 50-70% confluency were transfected by overnight incubation with the indicated cDNAs in complete growth medium using TransIT®-LT1 - Transfection Reagent according to the manufacturer’s instructions (Mirus Bio ...
-
No products found
because this supplier's products are not listed.
Simon J. Cleary, et al.,
bioRxiv - Immunology 2024
Quote:
... These sequences were codon-optimized and antibodies were expressed as chimeric hIgG1 with or without Fc point mutations in a HEK293 cell system and purified using protein A and buffer exchange (Absolute Antibody).
-
Cat# HY-P71023-10 μg,
10 μg, USD $80.0
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Yang Meng, et al.,
bioRxiv - Microbiology 2022
Quote:
... 100 μg/ml or 200 μg/ml 5-FC (MedChemExpress), 0.5 μg/ml or 1.0 μg/ml Amp B (MedChemExpress ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Kenji Yamaguchi, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
-
No products found
because this supplier's products are not listed.
Nathalie Lecat-Guillet, et al.,
bioRxiv - Biophysics 2022
Quote:
The pcDNA plasmid encoding human mGlu2 with N-terminal FLAG- and SNAP-tags was a gift from Cisbio Bioassays (Perking Elmer ...
-
No products found
because this supplier's products are not listed.
Jessie Altieri, Klemens J. Hertel,
bioRxiv - Molecular Biology 2021
Quote:
... A metabolic labeling protocol was followed[6] and the experimental HEK293 cells were incubated in the presence of 40µM unphosphorylated 4-thiouridine (Cayman Chemical, CAY16373-5). Total RNA was harvested from the cells using Trizol (Ambion ...
-
No products found
because this supplier's products are not listed.
Nathalie P. de Wagenaar, et al.,
bioRxiv - Genetics 2023
Quote:
... 1% PS and 5ng/ml human recombinant TGF-β1 (Biovision, 4342-5). This medium was changed every 4 days and after 14 days ...
-
No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
-
No products found
because this supplier's products are not listed.
Natalia Sanchez de Groot, et al.,
bioRxiv - Systems Biology 2019
Quote:
... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
-
No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
-
No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
-
No products found
because this supplier's products are not listed.
Chen-yu Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... (5) VWF+gcEVs and 5 μg of human rADAMTS-13 (MyBioSource, USA); and (6 ...
-
No products found
because this supplier's products are not listed.
Jia Xuan Leong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
-
No products found
because this supplier's products are not listed.
Jonathan M. Goodwin, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were plated on 35 mm glass-bottomed dishes (Mattek, Ashland, MA). Images were acquired every 2 minutes using a spinning disk confocal microscope ...