-
No products found
because this supplier's products are not listed.
Maria Mytiliniou, et al.,
bioRxiv - Biophysics 2021
Quote:
... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
-
No products found
because this supplier's products are not listed.
Aleksandra Vuchkovska, et al.,
bioRxiv - Immunology 2022
Quote:
... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
-
No products found
because this supplier's products are not listed.
Thiago J. Borges, et al.,
bioRxiv - Immunology 2023
Quote:
... 5 µg/ml of recombinant mouse Siglec-E-Fc chimera (R&D Systems, Cat. 5806) in 100 μl of buffer (10mM Tris-HCl (pH7.4) ...
-
No products found
because this supplier's products are not listed.
Guilherme Meira Lima, et al.,
bioRxiv - Biochemistry 2023
Quote:
... for all experiments except for experiment with soluble Siglec-7-Fc constructs in which a 5-laser Fortessa X-20 (BD Biosciences) was used instead.
-
No products found
because this supplier's products are not listed.
Dominik Soll, et al.,
bioRxiv - Immunology 2023
Quote:
... FCS was replaced with 5% human serum (Sigma-Aldrich, H6914). Hypersalinity (high NaCl ...
-
No products found
because this supplier's products are not listed.
Ian Tietjen, et al.,
bioRxiv - Microbiology 2021
Quote:
... was incubated with 5 nM HIS-tagged human PD-1 (Sino Biological) in the presence of 5 μg/mL protein A AlphaScreen acceptor bead and 5 μg/mL nickel chelate donor bead in a total volume of 10 μL of 20 mM HEPES (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
P.K Smitha, et al.,
bioRxiv - Molecular Biology 2022
Quote:
IgGFc: Human IgG Fc Sequence (Supplementary Material Figure S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
-
No products found
because this supplier's products are not listed.
Mélanie Bodnar-Wachtel, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... pcDNA3.1-Flag-His-ATM (Addgene 31985), pcDNA3.1-mCherry-NLRP3 ...
-
No products found
because this supplier's products are not listed.
Joanne Durgan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
-
No products found
because this supplier's products are not listed.
Megi Rexhepaj, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Anti-human Fc (Jackson ImmunoResearch) was diluted 1:50,000 in Intercept (TBS ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
-
No products found
because this supplier's products are not listed.
Trang H. Pham, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Myc-Flag-human TEADs (OriGene) and V5-ubiquitin (generated in house ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... anti-human IgA Fc or anti-human IgM Fc (Bethyl Labs, Montgomery, TX) at 2μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Kun Meng, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and 6×His-Flag-Ub was conducted using Ni-NTA agarose (Qiagen), following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Judith F. Ashouri, et al.,
bioRxiv - Immunology 2019
Quote:
... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively.
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Wei-Li Ling, Samuel Ken-En Gan,
bioRxiv - Immunology 2022
Quote:
KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
No products found
because this supplier's products are not listed.
Chao Gao, et al.,
bioRxiv - Biochemistry 2020
Quote:
... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
-
No products found
because this supplier's products are not listed.
Guilherme Meira Lima, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
-
No products found
because this supplier's products are not listed.
Michael A Margreiter, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
-
No products found
because this supplier's products are not listed.
Awital Bar Barroeta, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Medium containing 5% FCS (Lonza) was used to expand and grow cells in a Cell Factory (6320 cm2 ...
-
No products found
because this supplier's products are not listed.
Héctor Castillo, et al.,
bioRxiv - Genomics 2023
Quote:
... 5 µL Tn5 transposase (Illumina Cat FC-121-1030) and 22,5 µL nuclease-free H2O and incubated at 37 °C for 30 mins ...
-
No products found
because this supplier's products are not listed.
Nicole Marquardt, et al.,
bioRxiv - Immunology 2020
Quote:
... Siglec-7 (5-386, Alexa Fluor 488, Bio-Rad), TIM-3 (7D3 ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
No products found
because this supplier's products are not listed.
Aleksandra Vuchkovska, et al.,
bioRxiv - Immunology 2022
Quote:
... To generate a truncated version of Siglec-5 (pME- tSiglec-5) the ITIM and ITSM were excised from the pME-Siglec 5 plasmid with the restriction enzymes BbsI/MfeI (NEB, Ipswich, MA) and blunt ends were generated with DNA Polymerase I ...
-
No products found
because this supplier's products are not listed.
Dawn M. Wenzel, et al.,
bioRxiv - Biochemistry 2022
Quote:
(His)6-GST-SNX15 MIT was bound to cOmplete His-Tag purification beads (5 mL, Roche, Germany, 2h) and washed with 2 L wash buffer ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Catherine M. Mageeney, et al.,
bioRxiv - Microbiology 2020
Quote:
... Primary antibodies (Anti-FLAG: Sigma, F3165; Anti-His: Cell Signaling Technologies, Danver, MA, 2366S) were used at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Rebecca Garnham, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-Siglec-7 (Proteintech, 13939-1-AP, 1:200), anti-CD14 (Proteintech ...
-
No products found
because this supplier's products are not listed.
Annika Bestehorn, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... with modified pCW57.1 target vector in a ratio of 5:1:5:5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966) as transfection reagent ...
-
No products found
because this supplier's products are not listed.
Maeva Dupont, et al.,
bioRxiv - Cell Biology 2019
Quote:
... anti-Siglec-1 (Novus Biologicals) and anti-pSTAT1 (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Marco Di Gioia, et al.,
bioRxiv - Immunology 2023
Quote:
... His-tagged recombinant human AKT1 (BPS Bioscience, Cat# 40003), AKT2 (BPS Bioscience ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Nikita Subedi, et al.,
bioRxiv - Immunology 2022
Quote:
... 5% Human Serum (HS) (Sanquin), and 25 mM HEPES (Gibco ...
-
No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
-
No products found
because this supplier's products are not listed.
Juan A. Perez-Bermejo, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
-
No products found
because this supplier's products are not listed.
Oskar Marin-Bejar, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... FCS Express 5 Image Cytometry v.5.01.0029 (De Novo Software) was used to gate intensity thresholds for subsequent analysis ...
-
No products found
because this supplier's products are not listed.
Thiago J. Borges, et al.,
bioRxiv - Immunology 2023
Quote:
... and mouse IgM anti-mouse Siglec-E (clone F-7, Santa Cruz) were used as 1:200 ...
-
No products found
because this supplier's products are not listed.
Céline Christiansen, et al.,
bioRxiv - Microbiology 2022
Quote:
... 5 µg/ml human holo-transferrin (PAN Biotech) and 1.7 ng/µl sodium selenite (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Christina Vrettou, et al.,
bioRxiv - Immunology 2022
Quote:
... and blotted with anti-human IgG Fc (Jackson labs). (E ...
-
No products found
because this supplier's products are not listed.
Haizhang Chen, et al.,
bioRxiv - Immunology 2020
Quote:
... or a PE-TexasRed-conjugated human IgG-Fc fragment (Rockland) for 1h at 4°C in PBS/3%FCS ...
-
No products found
because this supplier's products are not listed.
Veronica Locher, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Kavita Rawat, Anita Tewari, Claudia V. Jakubzick,
bioRxiv - Cancer Biology 2020
Quote:
... 1 mg of anti-Siglec H (clone 440c Rat IgG2b, BioXcell), 1mg anti-mouse B220 ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...