-
No products found
because this supplier's products are not listed.
Pawel Stocki, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 100 µl of either a 1:5,000 peroxidase-conjugated anti-human Fc antibody or 1:20,000 streptavidin Poly-HRP40 Conjugate (Fitzgerald) diluted in 0.5% BSA in PBST was added and incubated for 1 hr ...
-
No products found
because this supplier's products are not listed.
Emma T Crooks, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by alkaline phosphatase labeled anti-human Fc conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT (Sigma).
-
No products found
because this supplier's products are not listed.
Jiachen Huang, et al.,
bioRxiv - Immunology 2021
Quote:
... 25 µl of secondary antibody (goat anti-human IgG Fc; Meridian Life Science) at a 1:4,000 dilution in blocking buffer was applied to each well for 1 hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Darya V. Voronina, et al.,
bioRxiv - Immunology 2022
Quote:
... [48] fused with human IgG1 Fc-fragment and Llama IgG2b hinge were purchased from Evrogen (Russia). CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio ...
-
No products found
because this supplier's products are not listed.
Bjoern Traenkle, et al.,
bioRxiv - Immunology 2021
Quote:
... an alpaca (Vicugna pacos) was immunized with the purified extracellular domains of human CD4 (aa26-390) recombinantly produced in HEK293 cells (antibodies-online GmbH, Germany). After initial priming with 1 mg ...
-
No products found
because this supplier's products are not listed.
Colten Lankford, Jon Houtman, Sheila Baker,
bioRxiv - Biochemistry 2021
Quote:
HEK293 cells were briefly washed with cold PBS prior to incubation with 2 mg/mL Sulfo-NHS-SS-Biotin (APExBIO cat. # A8005) in cold PBS for thirty minutes on ice ...
-
No products found
because this supplier's products are not listed.
Agnes Ulfig, et al.,
bioRxiv - Biochemistry 2019
Quote:
α2-Macroglobulin was purified from human plasma (obtained from Zen-Bio, Inc. ...
-
This Fibronectin solution has been purified from human plasma where it is found as a dimer and...
Cat# 5050-1MG,
1 mg, USD $135.0
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Lauren J. Lahey, et al.,
bioRxiv - Biochemistry 2020
Quote:
HEK293 cell lines were seeded in PurCol-coated (Advanced BioMatrix) 6-well plates at 300,000 total cells in 2 mL media one day before transfection ...
-
No products found
because this supplier's products are not listed.
Rasmus Riemer Jakobsen, et al.,
bioRxiv - Microbiology 2021
Quote:
The human colon adenocarcinoma cell line Caco-2 (ATTC HTB-37, LGC standards, Middlesex, UK) at passage 53 was grown in Dulbecco’s Modified Eagles Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Shuangfen Tao, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The ELISA for Bcl-2 was performed using a human Bcl-2 ELISA kit (LS-F4134, LSbio Lifespan Biosciences Inc, Seattle, WA, USA).
-
No products found
because this supplier's products are not listed.
Silvia De Cicco, et al.,
bioRxiv - Neuroscience 2020
Quote:
NFAT Reporter - HEK293 Cell Line (PKC/ Ca2+ Pathway) cell lines were purchased from Tebu-Bio and cultured in Dulbecco’s MEM (Merck ...
-
No products found
because this supplier's products are not listed.
Djem U. Kissiov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... In all cases medium contained 5% FCS (Omega Scientific), 0.2 mg/mL glutamine (Sigma) ...
-
No products found
because this supplier's products are not listed.
Franziska Nadler, et al.,
bioRxiv - Biochemistry 2022
Quote:
Transient siRNA-mediated knockdown was performed by transfecting HEK293 cells with 33 nM siRNA oligonucleotides (Eurogentec) targeting the transcript of interest (see Supplementary Table S1 ...
-
No products found
because this supplier's products are not listed.
Siyi Gu, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK293 cells expressing Flag-SNAP-CCR5 were seeded onto 10-mm glass-bottom dishes (FluoroDish, FD3510, WPI) previously coated with 5 μg/ml fibronectin ...
-
No products found
because this supplier's products are not listed.
Apirat Chaikuad, et al.,
bioRxiv - Biochemistry 2019
Quote:
... the 5 uL kinase reaction was performed with 2 ug/mL recombinant human ULK1 protein (1-649, SignalChem #U01-11G) and 80 ug/mL myelin basic protein (MBP ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human wild-type or mutant (K4M) histone H3.1 were cloned into pCDH-EF1-MCS-IRES-RFP (System Biosciences, CD531A-2). To express sgRNAs ...
-
No products found
because this supplier's products are not listed.
Alexandra V. Vylegzhanina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 1 hour at room temperature or 2) rabbit anti-human ORF1p polyclonal serum in 1% non-fat dry milk (RPI) in TBST in dilution 1:1,000 ...
-
No products found
because this supplier's products are not listed.
Francesca Curreli, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... Blot 1 and Blot 2 were immuno-detected with a human anti-ACE2 mAb (AC384) (Adipogen Life Sciences, San Diego, CA). The ECL Mouse IgG ...
-
No products found
because this supplier's products are not listed.
Niko Schwenzer, et al.,
bioRxiv - Cell Biology 2024
Quote:
HEK293 cells with constitutive expression of CaV subunits β3 and α2δ1 and inducible expression of α1D (Charles River Laboratories CT6232) were cultured in DMEM/F12 medium containing selection antibiotics and 0.6 µM isradipine (Sigma I6658) ...
-
No products found
because this supplier's products are not listed.
Alaullah Sheikh, et al.,
bioRxiv - Microbiology 2022
Quote:
... in vitro grown differentiated polarized monolayers of human ileal enteroid samples as well as mouse intestinal biopsy samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Ted Pella, Inc., Redding, CA) in 100 mM sodium cacodylate buffer ...
-
No products found
because this supplier's products are not listed.
Indira Wu, Hee Shin Kim, Tuval Ben-Yehezkel,
bioRxiv - Genomics 2019
Quote:
... while human liver total RNA and human blood total RNA were purchased from Zyagen. LoopSeq Transcriptome kit was obtained from Loop Genomics ...
-
No products found
because this supplier's products are not listed.
Marin Boutonnet, et al.,
bioRxiv - Neuroscience 2023
Quote:
... human angiotensin II (HelloBio®); N-Methyl-D-Aspartate (NMDA ...
-
No products found
because this supplier's products are not listed.
Damian Dudka, R. Brian Akins, Michael A. Lampson,
bioRxiv - Cell Biology 2023
Quote:
... Centromeres were labeled with CREST (human anti-human Anti-Centromere Antibody, 1:200, Immunovision, HCT-0100) and an Alexa Fluor 594–conjugated goat anti-human secondary antibody (ThermoFisher ...
-
No products found
because this supplier's products are not listed.
Md Ehtashamul Haque, et al.,
bioRxiv - Bioengineering 2021
Quote:
... in human plasma (Cambridge Bioscience, UK) to make samples of 1 ...
-
No products found
because this supplier's products are not listed.
S.G. Badman, et al.,
bioRxiv - Microbiology 2020
Quote:
... Human genomic DNA purchased from Bioline Australia (Cat No ...
-
No products found
because this supplier's products are not listed.
Sherwin P. Montano, et al.,
bioRxiv - Biochemistry 2021
Quote:
... [Ta6Br12]2+ * 2 Br– (Jena Bioscience) for 1 to 5 days ...
-
No products found
because this supplier's products are not listed.
Yan Tang, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Human and mouse MDK ELISA assays were performed using Human Midkine ELISA Kit PicoKine™ (EK1235, BOSTER) and Mouse MDK / Midkine (Sandwich ELISA ...
-
No products found
because this supplier's products are not listed.
Katharina Hoette, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Isolation of organoids from the embedding matrix (human hepatic organoids: Matrigel, Corning; human pancreatic organoids: Cultrex BME2, Amsbio) for fixation and whole-mount staining was performed with slight modifications of protocols published by Broutier et al ...
-
No products found
because this supplier's products are not listed.
Matthew D. J. Dicks, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Human coagulation Factor X (hFX) (Haematologic Technologies) was added to diluted vectors at a final concentration of 8 μg/mL ...
-
No products found
because this supplier's products are not listed.
Joelle P. Straehla, et al.,
bioRxiv - Bioengineering 2021
Quote:
Human iPS-ECs (Fujifilm Cellular Dynamics, 11713), human brain PCs and ACs (ScienCell) ...
-
No products found
because this supplier's products are not listed.
Yuliya Kurlishchuk, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and 20 ng/ml human EGF (Biomol). All cells were kept at 5% CO2 ...
-
No products found
because this supplier's products are not listed.
José Miguel Arcas, et al.,
bioRxiv - Neuroscience 2024
Quote:
Whole-cell voltage- and current-clamp recordings were obtained from mouse DRG neurons or transiently transfected HEK293 cells with borosilicate glass patch-pipettes (Sutter Instruments, 4-8 MΩ resistance) and were performed simultaneously with temperature recordings ...
-
No products found
because this supplier's products are not listed.
Jianfang Li, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Human C-peptide levels in isolated plasma were quantified using the STELLUX Chemi Human C-peptide ELISA kit (ALPCO Diagnostics) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Veronica Jové, et al.,
bioRxiv - Cancer Biology 2023
Quote:
The open reading frame DNA sequences of human USP18(AA16-372) (Uniprot Q9UMW8) and human pro-ISG15(AA1-165)C78S (Uniprot P05161) were synthesized by GENEWIZ. C78S was substituted to stabilize ISG15 for purification (Narasimhan ...
-
No products found
because this supplier's products are not listed.
Tingting Lan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2%DMSO (Amresco), 200μM Butylated hydroxyanisole (Sigma-Aldrich) ...
-
Pinatuzumab (Anti-Siglec-2 / CD22)a humanized IgG1 monoclonal antibody targeting CD22. It has...
Cat# A3059, SKU# A3059-1mg*25,
1mg*25, $5070.00
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Georgios Kotsaris, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... cells were starved for 5h in high glucose DMEM with 0% FCS and then cells were pre-treated for 1h with 0.5 μM SB431542 (Selleck chemicals). SB431542 medium was aspirated ...
-
No products found
because this supplier's products are not listed.
Lisa M. Røst, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 1,213C,2- or U13C6-glucose (2 g/l, 99%, Cambridge Isotope Laboratories) and incubated over night before treatment ...
-
No products found
because this supplier's products are not listed.
Du-Hwa Lee, et al.,
bioRxiv - Plant Biology 2022
Quote:
... anti-GAPC1/2 (Agrisera, AS15 2894 ...
-
No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
-
No products found
because this supplier's products are not listed.
Udoka C. Nwangwu, et al.,
bioRxiv - Zoology 2024
Quote:
... 15 ml Fetal Calf Serum (FCS) 3% and 5ml Amphotericin B) and 500ml of Zirconia beads (Firma Biospec: 2.0 mm, Cat. No 1107912). The contents were macerated using the Qiagen Tissuelyser LT for 10 min and then centrifuged at 4,500 x g for 15 min ...
-
No products found
because this supplier's products are not listed.
Marie Tolkiehn, Simon R. Schultz,
bioRxiv - Neuroscience 2019
Quote:
... and anaesthetised with isoflurane (2% for induction, 1 - 2% for surgery, 1% for electrophysiology in 1.2% O2, Harvard Apparatus, UK). To maintain clear airways and avoid tracheal secretions ...
-
No products found
because this supplier's products are not listed.
Alexander B. Coley, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Human embryonic kidney (HEK293) cell line was obtained from GenLantis (San Diego, CA) and cultured in MEM (Mediatech ...
-
No products found
because this supplier's products are not listed.
Pavel Shekhtmeyster, et al.,
bioRxiv - Neuroscience 2021
Quote:
... This vector was co-transfected into HEK293-AAV cells (Vector Biolabs) along with a pAdeno-helper vector and a pRC-AAV9 rep-cap plasmid ...
-
No products found
because this supplier's products are not listed.
Amrita Sule, et al.,
bioRxiv - Cancer Biology 2021
Quote:
YFP-ATM was immunoprecipitated from stably transfected HEK293 cells by GFP-TRAP (Chromotek). The immunoprecipitates were suspended in kinase assay buffer containing 2 μCi of [γ-32P] ATP ...
-
No products found
because this supplier's products are not listed.
Anselmo Canciani, et al.,
bioRxiv - Biochemistry 2020
Quote:
All NT and Agrin constructs were produced via transient transfection of SFM-HEK293 (Expression Systems) cell suspension cultures adapted to grow in a serum-free medium (ESF ...
-
No products found
because this supplier's products are not listed.
Lindsay P. Winkenbach, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... from Stellaris LGC (Biosearch Technologies, BNS-5082 and FC-1065, respectively). Secondary ...
-
No products found
because this supplier's products are not listed.
Alexa Schuettenberg, et al.,
bioRxiv - Immunology 2022
Quote:
... normal human IgG (ProSci #5503) on each plate as a positive control ...
-
No products found
because this supplier's products are not listed.
Vignesh Kasinath, et al.,
bioRxiv - Biophysics 2020
Quote:
... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
-
No products found
because this supplier's products are not listed.
Evangelos Stefanidis, et al.,
bioRxiv - Bioengineering 2023
Quote:
... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
-
No products found
because this supplier's products are not listed.
Weiwei Yang, et al.,
bioRxiv - Microbiology 2023
Quote:
... 2-amino-2′deoxyadenosine was purchased from Toronto Research Chemicals (North York, ON, Canada). Analytical reverse-phase HPLC was performed on an Agilent 1200 series LC/MS system equipped with a G1315D diode array UV-Vis detector and a 6120 quadrupole LC/MS detector in both positive (+ESI ...