-
No products found
because this supplier's products are not listed.
Ananth Pallaseni, et al.,
bioRxiv - Genetics 2023
Quote:
... with 15% FCS (ThermoFisher), 0.1 mM beta-mercaptoethanol,100 U/ml penicillin and 100 mg/ml streptomycin (Gibco) ...
-
No products found
because this supplier's products are not listed.
Aleksandra Vuchkovska, et al.,
bioRxiv - Immunology 2022
Quote:
... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
-
No products found
because this supplier's products are not listed.
Philip Schmassmann, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... live/dead stained and incubated with recombinant human Siglec-9 Fc chimera (#1139-SL-050) or recombinant mouse Siglec-E Fc chimera (#5806-SL-050) both from R&D systems, USA for 20 min on ice ...
-
No products found
because this supplier's products are not listed.
Clarissa M Koch, et al.,
bioRxiv - Immunology 2022
Quote:
... Siglec F (BD), Ly6C (eBioscience) ...
-
No products found
because this supplier's products are not listed.
Sophie G Kellaway, et al.,
bioRxiv - Genomics 2020
Quote:
... 15% FCS (Sigma ES-009), 100 units/ml penicillin ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Megi Rexhepaj, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Anti-human Fc (Jackson ImmunoResearch) was diluted 1:50,000 in Intercept (TBS ...
-
No products found
because this supplier's products are not listed.
Ying Fu, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
Nanobody-Fc format were produced through CRO company using HEK293 transient expression system (Sino Biological). Spike protein was expressed and purified using the method published previously (22) ...
-
No products found
because this supplier's products are not listed.
Rachana R. Chandran, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... rabbit anti- Siglec-F (1:200, Abcam), rabbit anti-ADRP (1:200 ...
-
No products found
because this supplier's products are not listed.
P.K Smitha, et al.,
bioRxiv - Molecular Biology 2022
Quote:
IgGFc: Human IgG Fc Sequence (Supplementary Material Figure S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
-
No products found
because this supplier's products are not listed.
George N. Llewellyn, et al.,
bioRxiv - Microbiology 2023
Quote:
... mouse-anti-human IgG Fc-HRP (Southern Biotech) was diluted 1:2000 in blocking buffer and added and plates were incubated for 2 hours at 370C ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Fynn M. Hansen, et al.,
bioRxiv - Systems Biology 2020
Quote:
HEK293 (human, DMSZ, ACC 635) and U2OS (human, American Type Culture Collection [ATCC], HTB-96) cell were cultivated in DMEM (Gibco ...
-
No products found
because this supplier's products are not listed.
Vincent Grass, et al.,
bioRxiv - Microbiology 2021
Quote:
... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
-
No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... anti-human IgA Fc or anti-human IgM Fc (Bethyl Labs, Montgomery, TX) at 2μg/ml in PBS ...
-
No products found
because this supplier's products are not listed.
Connor H. Ludwig, et al.,
bioRxiv - Systems Biology 2022
Quote:
... pooled with 15% PhiX Control v3 (Illumina #FC-110-3001), and sequenced one of the following ways ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Guilherme Meira Lima, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
-
No products found
because this supplier's products are not listed.
Katerina Jerabkova, et al.,
bioRxiv - Cell Biology 2020
Quote:
... human polyclonal CREST (Antibodies Incorporated, 15 234), rabbit polyclonal Aurora B (Abcam ab2254) ...
-
No products found
because this supplier's products are not listed.
Laura Alonso-Herranz, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
-
No products found
because this supplier's products are not listed.
Wei-Li Ling, Samuel Ken-En Gan,
bioRxiv - Immunology 2022
Quote:
KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
No products found
because this supplier's products are not listed.
Sangsin Lee, et al.,
bioRxiv - Neuroscience 2022
Quote:
... including the human IgG1 Fc (Addgene #145165), and mouse IgG1 Fc (Addgene #28216 ...
-
No products found
because this supplier's products are not listed.
Nicole Marquardt, et al.,
bioRxiv - Immunology 2020
Quote:
... Siglec-7 (5-386, Alexa Fluor 488, Bio-Rad), TIM-3 (7D3 ...
-
No products found
because this supplier's products are not listed.
Magdalena Oroń, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
No products found
because this supplier's products are not listed.
Jérôme Wahis, et al.,
bioRxiv - Neuroscience 2020
Quote:
... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
-
No products found
because this supplier's products are not listed.
Chao Gao, et al.,
bioRxiv - Biochemistry 2020
Quote:
... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
-
No products found
because this supplier's products are not listed.
Ivan K. Domingo, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... HEK293-Null2 cells were also co-transfected with a human MD-2 expression clone (OriGene, RC204686). JetPRIME (Polyplus ...
-
No products found
because this supplier's products are not listed.
Timo Vögtle, et al.,
bioRxiv - Biochemistry 2019
Quote:
... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
-
No products found
because this supplier's products are not listed.
Veronica Locher, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Pengfei Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
-
No products found
because this supplier's products are not listed.
Maeva Dupont, et al.,
bioRxiv - Cell Biology 2019
Quote:
... anti-Siglec-1 (Novus Biologicals) and anti-pSTAT1 (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Christina Vrettou, et al.,
bioRxiv - Immunology 2022
Quote:
... and blotted with anti-human IgG Fc (Jackson labs). (E ...
-
No products found
because this supplier's products are not listed.
Irene Fasciani, et al.,
bioRxiv - Cell Biology 2024
Quote:
HEK293 cells were seeded in 15 cm plates and transfected after 24h according to manufacturer protocols (Effectene, Qiagen) using 5 µg of plasmid DNA ...
-
No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Biochemistry 2023
Quote:
HEK293 cells were cultured in DMEM (PAN Biotech). All media were supplemented in with 10% FBS (PAN Biotech ...
-
No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
... HEK293 cells expressing human Angiotensin converting enzyme 2 (ACE2) (BPS Biosciences, Cat #79951) were cultured in EMEM containing 10% FBS and 5 mg/mL Puromycin to select for ACE2-expressing cells ...
-
No products found
because this supplier's products are not listed.
Fangzhou Lou, et al.,
bioRxiv - Immunology 2020
Quote:
... Normal Human Epidermal Keratinocytes (NHEKs; Lifeline Cell Technology, cat. FC-0007) stored in liquid nitrogen were defrosted and cultured in serum-free basal medium with growth factors (Lifeline Cell Technology ...
-
No products found
because this supplier's products are not listed.
Christopher D. Greer, et al.,
bioRxiv - Immunology 2021
Quote:
... The membrane was then washed again 3×15 minutes in PBST before visualizing on the Odyssey Fc imaging system (LI-COR) using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
M. Kröger, et al.,
bioRxiv - Biophysics 2021
Quote:
Peripheral blood monocytes were isolated from human blood using 15 Ml Ficoll-Paque (VWR, Darmstadt, Germany) centrifugation gradient ...
-
No products found
because this supplier's products are not listed.
Harman Malhi, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-Human Fc biosensors (ForteBio, catalog #18-0015) were submerged in wells of black 96-well microplates (Greiner, catalog #655209) containing 250 μL of kinetics buffer (PBS ...
-
No products found
because this supplier's products are not listed.
Jinwook Choi, et al.,
bioRxiv - Cell Biology 2020
Quote:
Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
-
No products found
because this supplier's products are not listed.
Shengyun Ma, et al.,
bioRxiv - Immunology 2022
Quote:
... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Masako Kohyama, et al.,
bioRxiv - Microbiology 2022
Quote:
... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Berislav Bošnjak, et al.,
bioRxiv - Immunology 2022
Quote:
... FCS files were analyzed using FCS Express V7 (De Novo Software) or FlowJo V10 (BD).
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... Human recombinant ACE2-Fc-tag (Raybiotech) was then added at 1 μg/mL and incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Kavita Rawat, Anita Tewari, Claudia V. Jakubzick,
bioRxiv - Cancer Biology 2020
Quote:
... 1 mg of anti-Siglec H (clone 440c Rat IgG2b, BioXcell), 1mg anti-mouse B220 ...
-
No products found
because this supplier's products are not listed.
Thiago J. Borges, et al.,
bioRxiv - Immunology 2023
Quote:
... and mouse IgM anti-mouse Siglec-E (clone F-7, Santa Cruz) were used as 1:200 ...
-
No products found
because this supplier's products are not listed.
Xaria X. Li, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... HEK293 cells were transiently transfected with β-arrestin 2-Renilla luciferase 8 (Rluc8) and human C5aR2-Venus constructs using XTG9 (Roche, Sydney, Australia) for 24 h ...
-
No products found
because this supplier's products are not listed.
Kuan-Ting Huang, et al.,
bioRxiv - Biochemistry 2022
Quote:
Fluorescence stereomicroscope (e.g., Leica M165 FC)