-
No products found
because this supplier's products are not listed.
Clarissa M Koch, et al.,
bioRxiv - Immunology 2022
Quote:
... Siglec F (BD), Ly6C (eBioscience) ...
-
No products found
because this supplier's products are not listed.
Dmitri I. Kotov, et al.,
bioRxiv - Immunology 2022
Quote:
... Siglec F (S17007L, BioLegend), CSF1R (AFS98 ...
-
No products found
because this supplier's products are not listed.
Seung Kuk Park, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 15 mL 6x His-Tag Monoclonal antibody (MA121315; Invitrogen) diluted 1:1500 in blocking solution supplemented with 0.1% NaN3 was added to the membrane and incubated at 4°C on an orbital shaker at 55 rpm for 14-16 h ...
-
No products found
because this supplier's products are not listed.
Caitlin M. MacCarthy, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... cynomolgus and porcine fibroblasts were cultured in high-glucose DMEM (Sigma) supplemented with 15% FBS ...
-
No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Recombinant cynomolgus IL 11 (cIL11, 90925-CNCE, Sino Biological), human IL11 (hIL11 ...
-
No products found
because this supplier's products are not listed.
Maya Shofa, et al.,
bioRxiv - Microbiology 2023
Quote:
... and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB ...
-
No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and cynomolgus IL11 were measured on Biacore T200 (GE Healthcare). VVB011 was immobilised on an anti-mouse capture chips and a concentration range of each of the analytes (hIL11 ...
-
No products found
because this supplier's products are not listed.
Philip Schmassmann, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... live/dead stained and incubated with recombinant human Siglec-9 Fc chimera (#1139-SL-050) or recombinant mouse Siglec-E Fc chimera (#5806-SL-050) both from R&D systems, USA for 20 min on ice ...
-
No products found
because this supplier's products are not listed.
Lisa Leib, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... 15 µl Hi-PerFect Transfection Reagent (Qiagen; #301705) and 60 µl of siRNA mixture (1 µM ...
-
No products found
because this supplier's products are not listed.
Rachana R. Chandran, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... rabbit anti- Siglec-F (1:200, Abcam), rabbit anti-ADRP (1:200 ...
-
No products found
because this supplier's products are not listed.
Krystian A. Kozek, et al.,
bioRxiv - Genetics 2020
Quote:
HEK293 cells were grown in alpha MEM (#15-012-CV, Corning) with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Pedro P. Cunha, et al.,
bioRxiv - Immunology 2022
Quote:
... 5×106 HEK293 cells were plated in 15 cm petri dishes and transfected the day after with 50 μL FuGENE (E2311, Promega), 10 μg CAR-encoding vectors and 3.3 μg of each 3rd generation lentivirus helper vectors (CART-027CL ...
-
No products found
because this supplier's products are not listed.
Rosemary A. Bamford, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and 1x penicillin/streptomycin (pen/strep; PAA) and distributed in 6-well plates with HEK293 cells transfected with Neo1.a-AP-His (Addgene #71963) or pcDNA3.1-CNTN4-FLAG ...
-
No products found
because this supplier's products are not listed.
K.K. Khoo, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
-
No products found
because this supplier's products are not listed.
Kyle Symonds, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Equivalent aliquots (15 µL) of each fraction were analysed by SDS-PAGE and immunoblotting using anti-His tag antibody (GenScript) overnight at 4°C as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Amy Q. Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and male Cynomolgus monkey plasma (BioIVT) using a Pierce Rapid Equilibration Dialysis (RED ...
-
No products found
because this supplier's products are not listed.
Ravi Jagasia, et al.,
bioRxiv - Neuroscience 2022
Quote:
NPCs derived from cynomolgus macaque (Cips_BC671A_NSC line generated at Roche) were differentiated in NPC differentiation medium (42) ...
-
No products found
because this supplier's products are not listed.
Rebecca Garnham, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-Siglec-7 (Proteintech, 13939-1-AP, 1:200), anti-CD14 (Proteintech ...
-
No products found
because this supplier's products are not listed.
Adrian Westhaus, et al.,
bioRxiv - Bioengineering 2022
Quote:
Cynomolgus and Rhesus macaque primary hepatocytes were purchased from Lonza (Cat# MKC118 and Cat# MKR103). Human hepatocytes were also purchased from Lonza (Cat# HUM181971 ...
-
No products found
because this supplier's products are not listed.
Shreya Ahuja, Iulia M. Lazar,
bioRxiv - Immunology 2021
Quote:
... TGFβ1 (HEK293 derived) and TGFβ2 (HEK293 derived) were acquired from Peprotech (Rocky Hill, NJ). Primary antibody [mouse monoclonal IgG anti-Vimentin (V9)] ...
-
No products found
because this supplier's products are not listed.
Ronald L. Shanderson, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
-
No products found
because this supplier's products are not listed.
Nicole Marquardt, et al.,
bioRxiv - Immunology 2020
Quote:
... Siglec-7 (5-386, Alexa Fluor 488, Bio-Rad), TIM-3 (7D3 ...
-
No products found
because this supplier's products are not listed.
Vinicius Martins de Oliveira, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
-
No products found
because this supplier's products are not listed.
Thiago J. Borges, et al.,
bioRxiv - Immunology 2023
Quote:
... and mouse IgM anti-mouse Siglec-E (clone F-7, Santa Cruz) were used as 1:200 ...
-
No products found
because this supplier's products are not listed.
Bashir A. Ali, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or anti-His (Cell Signaling #2366 His-Tag 27E8) primary antibodies ...
-
No products found
because this supplier's products are not listed.
Min He, et al.,
bioRxiv - Genomics 2024
Quote:
... The Hi-C libraries were amplified for 11–15 cycles with Q5 master mix (NEB, M0492L) following the operation manual ...
-
No products found
because this supplier's products are not listed.
Noelle Anderson, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... and sequencing (Illumina Hi-Seq) were performed by Iridian Genomes ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Benoît Henry, et al.,
bioRxiv - Physiology 2022
Quote:
... qPCRs were carried out in 15 μl in a 384-well plate using SensiFASTTM SYBR Hi-ROX kit (Bioline), 0·5 μM of each forward and reverse primer and 2·5 μl of template DNA ...
-
No products found
because this supplier's products are not listed.
Yoshifumi Abe, et al.,
bioRxiv - Neuroscience 2023
Quote:
... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
-
No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
-
No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
ACE2-HEK293s (BPS Bioscience) were cultured and transduced in opaque 96-well white plates (Corning® ...
-
No products found
because this supplier's products are not listed.
Kevin Michalski, et al.,
bioRxiv - Neuroscience 2019
Quote:
HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
-
No products found
because this supplier's products are not listed.
Krishnendu Chakraborty, et al.,
bioRxiv - Immunology 2021
Quote:
... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
-
No products found
because this supplier's products are not listed.
Brian L. Hie, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... S1-His or RBD-His were labelled using Monolith NTTM His-Tag Labeling Kit RED-tris-NTA (Nanotemper) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
-
No products found
because this supplier's products are not listed.
William J Dower, et al.,
bioRxiv - Immunology 2023
Quote:
... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
-
No products found
because this supplier's products are not listed.
Maeva Dupont, et al.,
bioRxiv - Cell Biology 2019
Quote:
... anti-Siglec-1 (Novus Biologicals) and anti-pSTAT1 (Cell Signaling Technology) ...
-
No products found
because this supplier's products are not listed.
Lie Wang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
No products found
because this supplier's products are not listed.
Jinwook Choi, et al.,
bioRxiv - Cell Biology 2020
Quote:
Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
-
No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
-
No products found
because this supplier's products are not listed.
Xiao Ma, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... HEK293/GC-A+ and HEK293/GC-B+ cell lines were generated from HEK293 parental cell transfected with plasmids (OriGene, Rockville, MD) containing either human GC-A or GC-B cDNA sequences ...
-
No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
-
No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
No products found
because this supplier's products are not listed.
Matthew R. Marunde, et al.,
bioRxiv - Genomics 2022
Quote:
... pAG-MNase (EpiCypher #15-1016) was added / activated and CUT&RUN enriched DNA purified using the Monarch DNA Cleanup kit (NEB #T1030S) ...
-
Leibovitz L-15 media powder, a component of the NCIS kit. Reconstitute entire contents of...
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Erin W. Meermeier, et al.,
bioRxiv - Immunology 2022
Quote:
... elastase (15 μg/mL, Worthington), trypsin I (1.5 μg/mL ...