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No products found
because this supplier's products are not listed.
Jasmine R. Anderson, et al.,
bioRxiv - Microbiology 2022
Quote:
... Sialic Acid was quantified using the Sialic Acid Quantitation Kit (Sigma), according to manufacturer’s directions.
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No products found
because this supplier's products are not listed.
Albertas Navickas, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Antibodies: beta-tubulin (Proteintech 66240-1-Ig), HNRNPC (Santa Cruz sc-32308) ...
-
No products found
because this supplier's products are not listed.
Robert G. Mealer, et al.,
bioRxiv - Neuroscience 2020
Quote:
Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Delphine M Depierreux, et al.,
bioRxiv - Microbiology 2021
Quote:
... cell surface sialic acid residues were oxidised and biotinylated using 1 mM sodium periodate (Thermo Scientific), 100 mM aminooxy-biotin (Biotium Inc. ...
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No products found
because this supplier's products are not listed.
Pascal Brandys, et al.,
bioRxiv - Immunology 2022
Quote:
... 5 ml of lectin beads (Galanthus nivalis lectin (GNL)-agarose beads (Vector Labs: AL-1243-5) were added to the filtered transfected supernatant and rocked overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Martin F. Peter, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Sialic acid (Carbosynth) was dissolved in the P-domain standard buffer (50 mM Tris ...
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No products found
because this supplier's products are not listed.
Brea K. Hampton, et al.,
bioRxiv - Genetics 2022
Quote:
... 96-well flat-bottom high-binding plates were coated with anti-Ig antibodies (Southern Biotech) diluted in carbonate buffer for each antibody subtype measured ...
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No products found
because this supplier's products are not listed.
Marzena Stefaniuk, et al.,
bioRxiv - Neuroscience 2021
Quote:
... binge-like drinking (BD group; n = 5), cue reexposure (CR group ...
-
No products found
because this supplier's products are not listed.
Satish Sagar, et al.,
bioRxiv - Microbiology 2020
Quote:
... and Sialic acids from the purified S-Ectodomain was performed by using a Deglycosylation kit (Agilent, CA, USA) as per the manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Aran Singanayagam, et al.,
bioRxiv - Immunology 2019
Quote:
... Mannose binding lectin (MBL)-2 (R&D Systems) and SLPI (R&D Systems).
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No products found
because this supplier's products are not listed.
Helena Hemberger, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... To digest sialic acid: 1.5μL of a2-3,6,8 Neuraminidase (50U/mL, New England Biolabs, NEB) with 1x GlycoBuffer 1 (NEB) ...
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No products found
because this supplier's products are not listed.
Zhengliang L Wu, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... The fluorophore conjugated CMP-sialic acids were then purified on a HiTrap® Q HP (GE Healthcare) column and eluted with a 0-100% gradient of NaCl elution buffer (150 NaCl ...
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No products found
because this supplier's products are not listed.
Marco Zaccaria, et al.,
bioRxiv - Biophysics 2023
Quote:
... 0.5% BSA and RBD-Fc binding was detected with goat-anti-human-Ig-APC (Jackson ImmunoResearch Laboratories, Pennsylvania, USA). Data was acquired using by flow cytometry using an LSRII (BD Biosciences ...
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No products found
because this supplier's products are not listed.
Tháyna Sisnande, et al.,
bioRxiv - Biochemistry 2019
Quote:
... anti-oligomer antibody (anti prefibrillar oligomer-like material; Cat #AB9234, Merck-Millipore) or anti-murine amylin (in-house derived ...
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No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Immunology 2022
Quote:
... PBMCs were first treated with the sialic acid inhibitor P-3FAX-Neu5Ac (Tocris) for 24 hours ...
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No products found
because this supplier's products are not listed.
Kaveri Banerjee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... FITC-Lectin (Bio-Rad) and MENA/ENAH (Sigma) ...
-
No products found
because this supplier's products are not listed.
Chao Gao, et al.,
bioRxiv - Biochemistry 2020
Quote:
... followed by optional sialic acid removal using 0.02 U sialidase (Roche), prior to in-gel trypsin treatment49 ...
-
No products found
because this supplier's products are not listed.
Woojong Lee, et al.,
bioRxiv - Immunology 2020
Quote:
... Trypsin-Like and Caspase-Like Cell-Based Assays (Promega), according to manufacturer’s instructions (Moravec et al. ...
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No products found
because this supplier's products are not listed.
Matthew L. Goodwin, et al.,
bioRxiv - Immunology 2020
Quote:
... and lectin resin (VWR) for gB Domain I ...
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No products found
because this supplier's products are not listed.
Arijita Chakraborty, et al.,
bioRxiv - Biochemistry 2022
Quote:
... per reaction were incubated with 200 μl antibody binding buffer [1X PBS/ 0.02% Tween 20] and 5 μg of anti-FMRP (Biolegend) and anti-FXR1(Santa Cruz Biotechnology #sc-374148) ...
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No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 5 nM Insulin-Like Growth Factor 1 (IGF1, Peprotech, 100-11), and 10 μM testosterone (Toronto Research Chemicals ...
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No products found
because this supplier's products are not listed.
Kezia Lizardo, et al.,
bioRxiv - Pathology 2020
Quote:
... Fatty acid binding protein (FABP)-4-specific rabbit monoclonal antibody (1:1000 dilution, D25B3, Cell Signaling), BNIP3 specific rabbit monoclonal antibody (1:1000 dilution ...
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No products found
because this supplier's products are not listed.
Aurelie Gueho, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... A custom-made antibody directed against peptide CKVGEPRGVSPEDMG of the sialic acid synthase (NCBI accession # XP_021429200) was purchased (GenScript U881EER070) and used at a dilution of 1/2000 ...
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No products found
because this supplier's products are not listed.
Åsa Ehlén, et al.,
bioRxiv - Cell Biology 2019
Quote:
The Polo-like binding domain (PBD) of PLK1 (amino acid 326 to amino acid 603) was amplified from the pTK24 plasmid (Addgene) and cloned into a pT7-His6-SUMO expression vector using NEB Gibson assembly (Gibson Assembly Master Mix ...
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No products found
because this supplier's products are not listed.
Alexandra Atalis, et al.,
bioRxiv - Immunology 2022
Quote:
Neutralizing antibodies were quantified like the ELISA method described above in a 384-well UltraCruz® ELISA high-binding plate (Santa Cruz Biotechnology). Diluted spike in 0.05 M carbonate-bicarbonate buffer (1 μg spike/mL ...
-
No products found
because this supplier's products are not listed.
Yoon-Hee Jung, et al.,
bioRxiv - Genomics 2022
Quote:
Adult (2-5 months old) CD-1 IGS mice (Charles River Lab, USA) were used for all experiments ...
-
No products found
because this supplier's products are not listed.
Eri Hirata, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 5-Fluoroorotic acid (5-FOA; ZYMO RESEARCH F9003), or doxycycline (Sigma D9891) ...
-
No products found
because this supplier's products are not listed.
Jeremy R. Egbert, et al.,
bioRxiv - Cell Biology 2019
Quote:
... antibody binding was detected using fluorescent secondary antibodies (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Shanshan Li, et al.,
bioRxiv - Biophysics 2021
Quote:
... Supernatant was collected for 2.5 h binding with Ni-nitrilotriacetic acid resins (Qiagen) at 4°C ...
-
No products found
because this supplier's products are not listed.
Manukumar Honnayakanahalli Marichannegowda, et al.,
bioRxiv - Microbiology 2020
Quote:
HIV-1 specific binding antibody titers were performed by coating high-binding 384 well plates (Corning, Corning, NY) overnight at 4°C with 1086C and Con6 gp120s ...
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No products found
because this supplier's products are not listed.
Claire E. Coupland, et al.,
bioRxiv - Biophysics 2021
Quote:
... and 5 mM valproic acid (Cayman Chemical). For biotinylated proteins ...
-
No products found
because this supplier's products are not listed.
Rasheduzzaman Rashu, et al.,
bioRxiv - Immunology 2023
Quote:
... with 50 μg of the Toll-like receptor (TLR)3 agonist polyinosinic-polycytidylic acid [poly (I:C)] (InvivoGen), 50 μg of the TLR7 agonist imiquimod (InvivoGen) ...
-
No products found
because this supplier's products are not listed.
Doris Roth, et al.,
bioRxiv - Biophysics 2024
Quote:
... samples were mounted upside down and cilia were live-stained with fluorescent-dye conjugated tomato lectin (IVISense™ Tomato Lectin 680, Perkin Elmer) by incubating the sample in a 0.25 µM dilution in PBS for 20 minutes ...
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No products found
because this supplier's products are not listed.
Kaito Nagashima, et al.,
bioRxiv - Immunology 2021
Quote:
... AviTagged Y2 COBRA HA proteins containing the Y98F mutation to reduce sialic acid binding were biotinylated using the BirA biotin-protein ligase in the BirA500 kit (Avidity) and complexed to streptavidin-fluorophores SA-PE (1:500 dilution ...
-
No products found
because this supplier's products are not listed.
Thusitha K. Karunarathna, et al.,
bioRxiv - Microbiology 2023
Quote:
Binding of virus and sialic receptor analogous was measured using an Octet Red biolayer interferometer (Pall FortéBio) as previously described (37) ...
-
No products found
because this supplier's products are not listed.
Stefanie Reuter, et al.,
bioRxiv - Immunology 2021
Quote:
... Additional to the NF-κB binding motifs NF-κB response element and a TATA-like sequence from pNFκB-Luc vector (Clontech Cat. No. 631904) were added to the Firefly luciferase gene and introduced into pCDH-CMV-EF1-Puro.
-
No products found
because this supplier's products are not listed.
Filip Brázdovič, et al.,
bioRxiv - Genomics 2023
Quote:
... eluted with 2 x 100 μl of 70 % (v/v) acetonitrile in 0.5 % (v/v) trifluoroacetic acid into low-binding microcentrifugation tubes (Protein LoBind, Eppendorf), and vacuum-dried in Vacufuge Concentrator Plus (Eppendorf) ...
-
No products found
because this supplier's products are not listed.
Simon J Moore, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... low-binding plate (Greiner). Reactions were measured as a triplicate technical repeat and at least repeated with cell-extracts prepared from two separate days ...
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Sialic acid Assay Kit (DSLA-100)
Cat# DSLA-100,
1.0 kit, 100 tests, USD $419.0
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Kaori Kobayashi, et al.,
bioRxiv - Immunology 2021
Quote:
The concentrations of total sialic acid (TSA) and free sialic acid (FSA) in saliva were measured using a sialic acid assay kit (Bioassay Systems). The concentration of protein-bound sialic acid (BSA ...
-
No products found
because this supplier's products are not listed.
Alexandra Lucas, et al.,
bioRxiv - Neuroscience 2021
Quote:
... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
-
No products found
because this supplier's products are not listed.
Juan J. Apiz-Saab, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Antigen-antibody binding was detected using Bond polymer refine detection (Leica Biosystems, DS9800). The tissue sections were counter stained with hematoxylin and covered with cover glasses.
-
No products found
because this supplier's products are not listed.
Miriam Kuhlmann, et al.,
bioRxiv - Immunology 2023
Quote:
Blocking of unspecific antibody binding was achieved with 2.4G2 (BioXCell) and dead cells were stained using the Zombie NIR™ Fixable Viability Kit (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Maricarmen Rojas-Lopez, et al.,
bioRxiv - Microbiology 2022
Quote:
... the secondary antibody used was TrueBlot horseradish peroxidase-conjugated anti-mouse Ig (Rockland, 18-8817-31). For detecting β-actin ...
-
No products found
because this supplier's products are not listed.
Lindsay Moritz, et al.,
bioRxiv - Biochemistry 2021
Quote:
... PNA-Lectin (GeneTex) was used to stain acrosomes and DAPI was used as a nuclear counterstain ...
-
No products found
because this supplier's products are not listed.
Huan Peng, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 5-bromosalicylic acid (5-BAA) (>98.0%; TCI), isopropyl β-D-1- thiogalactopyranoside (IPTG ...
-
No products found
because this supplier's products are not listed.
David Hoffmann, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
-
No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... anti-Insulin Like 3 (INSL3, Novus Biologicals, NBP1-81223) 1:500 ...
-
No products found
because this supplier's products are not listed.
Annette B. Vogel, et al.,
bioRxiv - Immunology 2020
Quote:
... Binding analysis of captured murine IgG antibodies to S1-His or RBD-His (Sino Biological) was performed using a multi-cycle kinetic method with concentrations ranging from 25 to 400 nM or 1.5625 to 50 nM ...
-
No products found
because this supplier's products are not listed.
Magdalena Kasendra, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Ascorbic Acid and 5% fetal bovine serum (Lonza Cat. no. CC-3202). At this time the medium supplying the epithelial channel was switched to differentiation medium ...
-
No products found
because this supplier's products are not listed.
Emily Hsieh, et al.,
bioRxiv - Microbiology 2022
Quote:
... for magnetic separation as described on the protocols.io website (https://doi.org/10.17504/protocols.io.bgztjx6n).Samples were then suspended in antibody binding buffer and split for overnight incubation with antibodies specific to panH4Ac (Active Motif; 39925), BRD4 (Cell Signaling ...