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No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
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WB,IF,IP,IHC,FC,ELISA
Cat# A5712, SKU# A5712-20ul,
20ul, $47.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Coralie Zangarelli, et al.,
bioRxiv - Genomics 2022
Quote:
A peptide corresponding to PgmL1 amino acid sequence 1 to 266 and carrying a C-terminal His tag was used for guinea pig immunization (Proteogenix). Sera were purified by antigen affinity purification to obtain highly specific α−PgmL1-GP antibodies (0.8 mg/mL) ...
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No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
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No products found
because this supplier's products are not listed.
Aleksandra Luginina, et al.,
bioRxiv - Biophysics 2023
Quote:
... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Mart M. Lamers, et al.,
bioRxiv - Microbiology 2021
Quote:
... and VSV nucleoprotein was stained using mouse-anti-VSV-N (1:1000, Absolute Antibody) followed by infrared-labelled secondary antibodies (1:20,000 ...
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No products found
because this supplier's products are not listed.
Hoa Quynh Do, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and lipid-protein nanodiscs (MSP1D1-His-POPC, MSP1D1-His-DMPG, MSP1D1-His-DMPC, Cube Biotech) were used to solubilize in-situ synthesized PCFT ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
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No products found
because this supplier's products are not listed.
Loyda M. Morales Rodríguez, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
HEK293 cells were plated onto 25mm coverslips (Electron Microscopy Sciences, #50949050) coated with poly-D-lysine (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... 1 μg SARS-CoV-2 Spike protein (antibodies-online, ABIN6952734) per lane was applied onto a 4-12% SDS polyacrylamide gradient gel ...
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No products found
because this supplier's products are not listed.
Bekir Altas, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 1 µM His-Ubiquitin (Boston Biochem, Cat#U-530), 50 nM mouse E1 enzyme ...
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No products found
because this supplier's products are not listed.
Guangda Shi, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 50 μM Phos-Tag reagent (Nard Institute AAL-107), and 100 μM MnCl2 ...
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No products found
because this supplier's products are not listed.
Thomas D. Avery, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
NRF2/ARE luciferase reporter HEK293 cells (SL-0042-NP, Signosis, Santa Clara, USA) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
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No products found
because this supplier's products are not listed.
Jiuzhi Xu, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Nucleoprotein was isolated from cells using a nucleoprotein extraction kit (AR0106, Bosterbio, USA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Satoshi Imanishi, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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No products found
because this supplier's products are not listed.
Sidar Aydin, et al.,
bioRxiv - Immunology 2021
Quote:
... or the (VSV) nucleoprotein peptide (RGYVYQGL, BACHEM, Bubendorf, Switzerland) at 0.1 ng/mL for 30 minutes at 37°C or left unpulsed ...
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No products found
because this supplier's products are not listed.
Frederik Fleissner, et al.,
bioRxiv - Biophysics 2020
Quote:
A plasmid coding for human vimentin containing a C-terminal His-tag (EX-D0114-B31, Tebu-bio, Germany) was transformed into E ...
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No products found
because this supplier's products are not listed.
Biao Zhou, et al.,
bioRxiv - Microbiology 2021
Quote:
... immunofluorescence staining was performed on deparaffinized and rehydrated tissue sections using a rabbit anti-SARS-CoV-2-NP protein antibody together with an AF488-conjugated anti-rabbit IgG (Jackson ImmunoResearch, PA, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Sammy M. Njenga, et al.,
bioRxiv - Epidemiology 2019
Quote:
... and recombinant measles nucleoprotein (MV-N, Meridian Life Sciences, Memphis, TN) [30] were purchased from commercial sources ...
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No products found
because this supplier's products are not listed.
FWN Chow, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Quantification was carried by western blot analysis in comparison to known concentrations of recombinant full length exWAGO containing an N-terminal 3XFlag-His tag using Image Studio Lite (LI-COR) software ...
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No products found
because this supplier's products are not listed.
Ashok Pabbathi, et al.,
bioRxiv - Biophysics 2021
Quote:
... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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No products found
because this supplier's products are not listed.
Kristin S. Fitzpatrick, et al.,
bioRxiv - Immunology 2022
Quote:
... SARS-CoV2YLQ (YLQPRTFLL, Biomatik) and Vaccinia virus B8R (TSYKFESV ...
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Recombinant Human CD28 (P10747-1) extracellular domain (Met 1-Pro 152), fused with a...
Cat# CD28-3910H,
50ug , USD $298
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Jonathan H. Shrimp, et al.,
bioRxiv - Biochemistry 2020
Quote:
Recombinant Human TMPRSS2 protein expressed from Yeast (human TMPRSS2 residues 106-492, N-terminal 6x His-tag) (Cat # TMPRSS2-1856H) was acquired from Creative BioMart (Shirley, NY). Peptides obtained from Bachem include ...
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No products found
because this supplier's products are not listed.
Sharon R. Stevens, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR(Ho et al. ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Martin P. Schwalm, et al.,
bioRxiv - Biochemistry 2023
Quote:
HEK293 cells were cultured in DMEM (PAN Biotech). All media were supplemented in with 10% FBS (PAN Biotech ...
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No products found
because this supplier's products are not listed.
Maria Kuzikov, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Inhibition of SARS-CoV PLpro was measured using 1 nM of SARS-CoV PLpro (Biomol, #SBB-DE0024) and 0.25 µM ISG15-AMC substrate ...
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No products found
because this supplier's products are not listed.
Lyra O. Randzavola, et al.,
bioRxiv - Immunology 2022
Quote:
... HEK293-F were transfected with polyethylenimine (Polyscience Europe GmbH) at a ratio of 1:3 DNA:polyethylenimine (Tom et al. ...
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No products found
because this supplier's products are not listed.
Maali AlAhmad, et al.,
bioRxiv - Cell Biology 2024
Quote:
... HEK293-TRPM2tet cells grown in 96-well plates (Sarstedt) were preloaded with 1 µM Fura-2-AM/ 0.02% Pluronic® F127 (P-3000MP ...
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No products found
because this supplier's products are not listed.
Alena Salašová, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cells were lysed for 30min shaking in NP lysis buffer (3M Tris-HCl pH8 ...
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No products found
because this supplier's products are not listed.
Hans-Georg Sprenger, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... MITO-Tag mice (The Jackson Laboratory #032290) were described earlier 16 and to generate whole body-expressing MITO-Tag mice ...
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No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
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No products found
because this supplier's products are not listed.
Jonathan M. Goodwin, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were plated on 35 mm glass-bottomed dishes (Mattek, Ashland, MA). Images were acquired every 2 minutes using a spinning disk confocal microscope ...
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No products found
because this supplier's products are not listed.
Camilla Margaroli, et al.,
bioRxiv - Pathology 2022
Quote:
... Anti-SARS-CoV-2 was conjugated to PE / R-Phycoerythrin (Expedeon Lightning-Link R-PE Conjugation Kit / Abcam ...
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No products found
Vivian Tam, et al.,
bioRxiv - Systems Biology 2020
Quote:
... AF and NP were then digested by 0.1% type II collagenase (Worthington Biochemical) for 14 hours and 0.05% type II collagenase for 8 hours ...
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No products found
because this supplier's products are not listed.
Lea Gaucherand, et al.,
bioRxiv - Microbiology 2022
Quote:
... 1% NP-40) supplemented with 50 μg/ml phenylmethylsulfonyl fluoride (PMSF; G-Biosciences) and cOmplete protease cocktail inhibitor (Roche) ...
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No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
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No products found
because this supplier's products are not listed.
Qiannan Zhang, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... and MBP (PurKine MBP-Tag Dextrin Resin 6FF, Abbkine) in turn ...
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No products found
because this supplier's products are not listed.
Satyaveni Malasala, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
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No products found
because this supplier's products are not listed.
Jason Derks, Nikolai Slavov,
bioRxiv - Systems Biology 2023
Quote:
... and mTRAQ-Δ8 mass tags (SciEx, 4440015, 4427698 and 4427700), respectively ...
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No products found
because this supplier's products are not listed.
Kui K. Chan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C ...
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No products found
because this supplier's products are not listed.
Kari H. Ecklund, et al.,
bioRxiv - Cell Biology 2021
Quote:
Anti-His-coated 0.44 μm microbeads (PSS4; Spherotech; prepared as described previously72) were incubated with purified 6His-GFP-3HA-GST-dynein331-HALO in dynein trapping buffer (30 mM HEPES pH 7.2 ...
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No products found
because this supplier's products are not listed.
Somboon Wankanit, et al.,
bioRxiv - Molecular Biology 2024
Quote:
A total of 350,000 HEK293-T cells were seeded in each well of 6-well plates (#EP0030720113, Eppendorf). Transfection was performed the day after at 40-50% cell confluence ...
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No products found
because this supplier's products are not listed.
Zhiqiang Zheng, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S-expressing plasmids were codon-optimized and generated by gene synthesis (Bio Basic Asia Pacific) according to GenBank accession QHD43416.1 ...