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No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
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No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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No products found
because this supplier's products are not listed.
Panpan Zhou, et al.,
bioRxiv - Immunology 2022
Quote:
... SARS-CoV-2 S protein with Avi-tag was conjugated to streptavidin-BV421 (BD Pharmingen cat.# 563259) and streptavidin-AF488 (Invitrogen cat.# S11223) ...
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No products found
because this supplier's products are not listed.
Artur Louche, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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No products found
because this supplier's products are not listed.
Saroj Khadka, et al.,
bioRxiv - Microbiology 2022
Quote:
... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
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No products found
because this supplier's products are not listed.
Hisashi Nomura, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were incubated with 10 μg of rDsg3-E-His in 100 μL of RPMI on ice for 1 h and stained with anti-E-tag-FITC (Bethyl Laboratories). After excluding dead cells by staining with 7AAD ...
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No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Microbiology 2019
Quote:
... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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No products found
because this supplier's products are not listed.
M. Florencia Sánchez, et al.,
bioRxiv - Biophysics 2022
Quote:
... A nosepiece stage (IX2-NPS, Olympus) was used for z-plane adjustment and drift minimization ...
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No products found
because this supplier's products are not listed.
Brian Belardi, et al.,
bioRxiv - Cell Biology 2019
Quote:
... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
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No products found
because this supplier's products are not listed.
Shenglan Shang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... the nucleoproteins were extracted and detected using the Trans-AM NF-κB kits (Active Motif, Carlsbad, CA, USA).
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No products found
because this supplier's products are not listed.
Robert V. Blair, et al.,
bioRxiv - Pathology 2020
Quote:
Samples collected at preinfection and weekly post-infection until necropsy were tested for detection of binding IgG antibodies against SARS-CoV-2 nucleoprotein (NP) by MFIA COVID-Plex from Charles River Laboratories ...
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SARS-CoV-2 (2019-nCoV), Recombinant Protein, Nucleocapsid linked Spike RBD Fusion Protein.
Cat# VReP-Wyb077,
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Sumit Sen Santara, et al.,
bioRxiv - Immunology 2021
Quote:
... Siglec6-His tag (Creative Biolabs), 4μ8C ...
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No products found
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
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No products found
because this supplier's products are not listed.
Ramona Rica, et al.,
bioRxiv - Immunology 2024
Quote:
... cells were stained with VL-4 rat anti-LCMV nucleoprotein (BioXCell), followed by staining with anti-rat horseradish peroxidase (Jackson Immunoresearch ...
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No products found
because this supplier's products are not listed.
Xi Zhang, et al.,
bioRxiv - Cell Biology 2024
Quote:
... or plastic-bottom (Ibidi: DLD1, HEK293) 96-well plates at densities targeting 60-70% confluence at the time of transfection ...
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No products found
because this supplier's products are not listed.
Wanlu Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
... TAG (triacylglycerol, D5-TAG-Mix, LM-6000/D5-TAG 17:0,17:1,17:1, Avanti Polar Lipids), PA (phosphatidic acid ...
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No products found
because this supplier's products are not listed.
Santosh Phuyal, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 0.5% NP-40) and GFP- trap (ChromoTek, #gta-100) was performed following manufacturer’s protocol.
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No products found
because this supplier's products are not listed.
Matthieu P.M.H. Benoit, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the SNAPf-EGFP-6His tag was replaced with a strep-II tag (IBA Lifesciences GmbH) using Q5 mutagenesis (New England Biolabs Inc. ...
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No products found
because this supplier's products are not listed.
James P Bridges, et al.,
bioRxiv - Cell Biology 2021
Quote:
HEK293 cells were seeded in 384-well plates (Greiner, #781946) at 30 000 cells/well ...
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No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2023
Quote:
... Env trimers with C-terminal avi-tags (Avidity) were biotinylated and conjugated to streptavidin labeled with different fluorochormes ...
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No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
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No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
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No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... anti-His tag (Solarbio Life Sciences, China, K200060M), anti-toll-like receptor-4 (TLR-4 ...
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No products found
because this supplier's products are not listed.
Saejeong Park, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... HEK293 cells were washed with PBS and lysed in NP-40 lysis buffer (Boston Bioproducts) including protease and phosphatase inhibitor (Roche) ...
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No products found
because this supplier's products are not listed.
Elizabeth R. Vanderwall, et al.,
bioRxiv - Immunology 2021
Quote:
... To measure SARS-CoV-2 replication in AEC cultures we used the Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit (Primerdesign®), with duplicate assays of harvested RNA from each SARS-CoV-2-infected AEC experimental condition ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... His-tagged peptide containing the SARS-CoV-2-specific furin motif (EpiGentek), and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
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No products found
because this supplier's products are not listed.
Juan Jauregui-Lozano, et al.,
bioRxiv - Genomics 2021
Quote:
CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
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No products found
because this supplier's products are not listed.
Annalisa Tito, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were then infected with SARS-CoV-2 Spike-Pseudotyped Lentivirus (Firefly Luciferase SARS-CoV-2 lentiviral particles-GeneCopoeia) and the control VSV-G protein pseudotyped Lentivirus (HLUC-Lv201 Firefly luciferase − eGFP lentifect-GeneCopoeia ...
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No products found
because this supplier's products are not listed.
Harold Marcotte, et al.,
bioRxiv - Immunology 2021
Quote:
... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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No products found
because this supplier's products are not listed.
Haijing Guo, Jen-Hsuan Wei, Joachim Seemann,
bioRxiv - Cell Biology 2020
Quote:
... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
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No products found
Wei Dong, et al.,
bioRxiv - Cell Biology 2020
Quote:
HEK293 cells were cultured in glass bottom dishes (In Vitro Scientific) and were transfected with DNA using X-treme Gene 9 DNA transfection reagent (Sigma Cat# 6365787001) ...
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No products found
because this supplier's products are not listed.
Jack M. O’Shea, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... and mouse anti-mCherry-Tag Monoclonal (Elabscience) at a dilution of 1:1000 for SGR58 ...
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No products found
because this supplier's products are not listed.
Lisa Dettinger, et al.,
bioRxiv - Microbiology 2021
Quote:
... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ...
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No products found
because this supplier's products are not listed.
Martina Sundqvist, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and Tag-lite SNAP-Lumi4-Tb from Cisbio Bioassays (Codolet ...
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No products found
because this supplier's products are not listed.
Stéphane Pillet, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV NP primary antibody (EastCoast Bio; ME, USA) and a Goat Anti-Mouse IgG conjugate antibody (Fitzgerald ...
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No products found
because this supplier's products are not listed.
The Nhu Nguyen, et al.,
bioRxiv - Microbiology 2023
Quote:
Antibody responses against IAV-S nucleoprotein (NP) were measured by using a commercial blocking ELISA (IDEXX, Montpellier, France), following the manufacturer’s recommendation ...
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No products found
because this supplier's products are not listed.
Renata Varnaitė, et al.,
bioRxiv - Immunology 2020
Quote:
... SARS-CoV-2 specific IgM antibodies were detected using EDI Novel Coronavirus COVID-19 IgM ELISA kit (Epitope Diagnostics), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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No products found
because this supplier's products are not listed.
Anil Verma, et al.,
bioRxiv - Immunology 2023
Quote:
... were labeled with biotinylated anti-His tag monoclonal antibody (ThermoScientific) and used to capture His-tagged clade C gp120 Du151 protein (Immune Technologies). The gp120-expressing beads were then incubated with triplicate 5-fold dilutions of heat-inactivated serum samples in V-bottom plates for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
Brenda M. Calderon, et al.,
bioRxiv - Microbiology 2019
Quote:
... IAV nucleoprotein monoclonal antibody (HT-103; catalog no. EMS010, Kerafast) at 1:1000 dilution ...
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No products found
because this supplier's products are not listed.
Dora Pinto, et al.,
bioRxiv - Immunology 2020
Quote:
... His-tagged RBD of SARS-CoV or SARS-CoV-2 were loaded at 3 µg/ml in KB for 15 minutes onto anti-HIS (HIS2) biosensors (Molecular Devices, ForteBio). Association of mAb and Fab was performed in KB at 15 μg/ml and 5 μg/ml respectively for 5 minutes ...
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No products found
because this supplier's products are not listed.
Julian A. Harris, et al.,
bioRxiv - Biochemistry 2021
Quote:
... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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No products found
because this supplier's products are not listed.
Liu He, et al.,
bioRxiv - Biochemistry 2022
Quote:
... coli strain BL21 (DE3) with an N-terminal (His)6-SUMO tag from a pE-SUMO-pro expression vector (LifeSensors). Cells were grown in 2YT media at 24°C to an OD600 of 0.8 and then induced with 0.1 mM isopropyl β-D-thiogalactopyranoside (IPTG ...
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No products found
because this supplier's products are not listed.
Shreya Ahuja, Iulia M. Lazar,
bioRxiv - Immunology 2021
Quote:
... TGFβ1 (HEK293 derived) and TGFβ2 (HEK293 derived) were acquired from Peprotech (Rocky Hill, NJ). Primary antibody [mouse monoclonal IgG anti-Vimentin (V9)] ...
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No products found
because this supplier's products are not listed.
Peter Vandeberg, et al.,
bioRxiv - Immunology 2020
Quote:
Anti-SARS-CoV-2 IgG titers were determined using Human Anti-SARS-CoV-2 Virus Spike 1 (S1) IgG assay (Alpha Diagnostic). hIVIG batches were tested using multiple serial dilutions and a curve constructed by plotting the log of the optical density as a function of the log of the dilution ...
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No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...