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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
Guillermo Valenzuela Nieto, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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No products found
because this supplier's products are not listed.
Lieve E.H. van der Donk, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV-2 pseudovirus productions were quantified by p24 ELISA (Perkin Elmer Life Sciences).
-
No products found
because this supplier's products are not listed.
Edward Sullivan, et al.,
bioRxiv - Microbiology 2021
Quote:
... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
-
No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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No products found
because this supplier's products are not listed.
Takeshi Katsuda, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and 1 μl E-Coli spike-in DNA (0.5 ng/μl) (EpiCypher) to each tube ...
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No products found
because this supplier's products are not listed.
Vania Passos, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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No products found
because this supplier's products are not listed.
Chenjian Gu, et al.,
bioRxiv - Microbiology 2020
Quote:
... pCAGGS-SARS-CoV-2-S that encodes the SARS-CoV-2 Spike gene was generated by GENEWIZ (Suzhou, China). Recombinant adenovirus expressing human ACE2 (Ad5-hACE2 ...
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No products found
because this supplier's products are not listed.
B Balakrishnan, K Lai,
bioRxiv - Biochemistry 2021
Quote:
... anti-SARS-CoV-2 Spike Protein was purchased from Origene Technologies Inc ...
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No products found
because this supplier's products are not listed.
Brett Stern, Peter Monteleone, Janet Zoldan,
bioRxiv - Bioengineering 2022
Quote:
Viral spike protein (either SARS-CoV-2 Spike Protein, S1 Subunit, RayBiotech, or MERS-CoV-1 Spike Protein, S1 Subunit, BioVision) was added to endothelial culture media of CD34+ iPSC-EP-laden hydrogels at a concentration of 10 μg/mL either one or five days after cell encapsulation ...
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No products found
because this supplier's products are not listed.
Annalisa Tito, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were then infected with SARS-CoV-2 Spike-Pseudotyped Lentivirus (Firefly Luciferase SARS-CoV-2 lentiviral particles-GeneCopoeia) and the control VSV-G protein pseudotyped Lentivirus (HLUC-Lv201 Firefly luciferase − eGFP lentifect-GeneCopoeia ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Niayesh Razi, et al.,
bioRxiv - Microbiology 2023
Quote:
The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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No products found
because this supplier's products are not listed.
Anwen Shao, Joseph L. Kissil, Chen-Ming Fan,
bioRxiv - Developmental Biology 2023
Quote:
... Brightfield microscope (Nikon Eclipse E 800), fluorescence microscope (Nikon E800) ...
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No products found
because this supplier's products are not listed.
Romain Rouet, et al.,
bioRxiv - Immunology 2020
Quote:
... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Alexandria N. Miller, et al.,
bioRxiv - Biophysics 2022
Quote:
... purified SARS-CoV-2 Orf3a was reconstituted in soybean polar lipid extract (Avanti Polar Lipids) at 1:10 and 1:100 wt:wt protein to lipid ratios ...
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No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 5 ng/mL human recombinant Platelet-Derived Growth Factor-AA (PDGF-AA, Peprotech, 100-13A) and 5 ng/mL FGF2 were added ...
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No products found
because this supplier's products are not listed.
Kevin W. Ng, et al.,
bioRxiv - Immunology 2021
Quote:
... mice were injected intraperitoneally with 25 µg recombinant SARS-CoV-2 S2 (CV2006; LifeSensors) in monophosphoryl lipid A adjuvant (Sigma Adjuvant System ...
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No products found
because this supplier's products are not listed.
Gabriela Mattoso Coelho, et al.,
bioRxiv - Microbiology 2023
Quote:
... washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000) (MP Biomedicals, Irvine, CA, USA) or the in-house anti-SARS-CoV-2 mAb (1/400) ...
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No products found
because this supplier's products are not listed.
Hoai Thi Thu Tran, et al.,
bioRxiv - Microbiology 2022
Quote:
SARS-CoV-2 Spike B.1.1.529 (Omicron) variant pseudotyped lentivirus particles were purchased from Biomol (Hamburg, Germany). The transduction assay was performed as described previously [12] ...
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No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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No products found
because this supplier's products are not listed.
Mehdi Moustaqil, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... coli C41(DE3) cells were transformed with the SARS-CoV-2 pET-28a-nsp5 plasmid (IDT DNA). Single colonies were used to inoculate LB media supplemented with kanamycin (50 μg/mL) ...
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No products found
because this supplier's products are not listed.
Tanay Bhatt, et al.,
bioRxiv - Pathology 2022
Quote:
About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
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No products found
because this supplier's products are not listed.
Dora Pinto, et al.,
bioRxiv - Immunology 2020
Quote:
... His-tagged RBD of SARS-CoV or SARS-CoV-2 were loaded at 3 µg/ml in KB for 15 minutes onto anti-HIS (HIS2) biosensors (Molecular Devices, ForteBio). Association of mAb and Fab was performed in KB at 15 μg/ml and 5 μg/ml respectively for 5 minutes ...
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No products found
because this supplier's products are not listed.
Bennett J. Davenport, et al.,
bioRxiv - Immunology 2021
Quote:
... and probed with 1 μg/mL of chimeric human anti-SARS-CoV spike antibody (CR3022, Absolute Antibody, Ab01680) in Perm Wash (1X PBS/0.1% saponin/0.1% BSA ...
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No products found
because this supplier's products are not listed.
Kristin Metzdorf, et al.,
bioRxiv - Immunology 2022
Quote:
... 3 μm thick FFPE sections were stained for SARS-CoV-2 with mouse-anti-nucleocapsid CoV-1/2 (Synaptic Systems, HS-452 11, clone 53E2, subtype: IgG2a) and for macrophages with rat-anti-mouse-MAC2 (Biozol Diagnostica/CEDARLANE,CL8942AP ...
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No products found
because this supplier's products are not listed.
Krishna Gupta, Elisha Krieg,
bioRxiv - Synthetic Biology 2024
Quote:
... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19/2019-nCoV) Spike...
Cat# abx600065-100UG,
100 µg USD $826.5
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Bálint Kiss, et al.,
bioRxiv - Biophysics 2020
Quote:
... 100 μl of 10 μg/ml SARS-CoV-2 Spike Glycoprotein Antibody (#abx376478, Abbexa Ltd, Cambridge, UK) was then added ...
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SARS-CoV, Recombinant Protein, Spike protein.
Cat# VReP-Wyb111,
1.0 case, Inquiry
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Xiaotian Tan, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337). The recombinant CR3022 therapeutic antibody was purchased from Creative Biolabs (MRO-1214LC) ...
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No products found
because this supplier's products are not listed.
Christopher J. Emig, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... SARS-CoV-2 Delta Variant pseudovirus (eEnzyme) was diluted 1:2 in DMEM complete media to a pseudoviral particle concentration of 5e7/ml ...
-
No products found
because this supplier's products are not listed.
Mariana F. Tioni, et al.,
bioRxiv - Immunology 2021
Quote:
Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Irina A. Favorskaya, et al.,
bioRxiv - Immunology 2021
Quote:
The RBD nucleotide sequence of SARS-CoV-2 Wuhan-Hu-1 isolate (Genbank accession number MN908947, from 319 to 545 aa) was synthesized (Evrogen, Russia) and cloned into the pCEP4 mammalian expression vector (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Wael Bahnan, et al.,
bioRxiv - Immunology 2021
Quote:
... making them permissive to SARS-CoV-2 infection (Jackson laboratories). One day after infection ...
-
No products found
because this supplier's products are not listed.
Kyung Ku Jang, et al.,
bioRxiv - Cell Biology 2022
Quote:
SARS-CoV-2-infected monolayers were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 3 hrs at RT ...
-
No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... and SARS-CoV-2 smFISH probes were designed using Stellaris smFISH probe designer (Biosearch Technologies) available online at http://www.biosearchtech.com/stellaris-designer ...
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No products found
because this supplier's products are not listed.
Bin Zhou, et al.,
bioRxiv - Microbiology 2020
Quote:
... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
-
No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
-
No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
-
No products found
because this supplier's products are not listed.
Mohamed Fareh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
-
Supplied as a dried powder purified from E. coli Type B cells (ATCC#11303) as described by...
Cat# LS004451,
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M. Alejandra Tortorici, et al.,
bioRxiv - Immunology 2021
Quote:
... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
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No products found
because this supplier's products are not listed.
Alexander L. Nielsen, et al.,
bioRxiv - Biochemistry 2020
Quote:
... from BPS Biosciences (San Diego, CA). SIRT5 (aa 37–310 with N-terminal His-tag, ≥95% purity; cat. #BML-SE555-0050) from Enzo Life Sciences (Farmingdale, NY). Purities were based on SDS-PAGE and Coomassie blue stain according to the supplier and all enzyme concentrations given were based on the stock concentrations determined by the supplier ...
-
No products found
because this supplier's products are not listed.
Meenakshi Kar, et al.,
bioRxiv - Immunology 2024
Quote:
... The impact of CD8+ T cell depletions on SARS-CoV-2 infection was also evaluated using anti-CD8β (clone 53-5.8, BioXCell) and isotype mAb IgG2b (clone LTF-2 ...
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No products found
because this supplier's products are not listed.
Anna C. Fagre, et al.,
bioRxiv - Microbiology 2020
Quote:
... Horizontal whole-lung slides of consecutive planes of SARS-CoV-2-infected and antibody-treated lungs were digitalized by Olympus histoslide scanner to create image data files that can be used for further digital image analysis (DIA) ...
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No products found
because this supplier's products are not listed.
Sakshi Gera, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... LPS-B5 (Lipopolysaccharide E. coli 055:B5, Calbiochem, Catalog # 437625,), LPS-B4 (LPS-EB ...
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No products found
because this supplier's products are not listed.
Biao Zhou, et al.,
bioRxiv - Microbiology 2021
Quote:
... immunofluorescence staining was performed on deparaffinized and rehydrated tissue sections using a rabbit anti-SARS-CoV-2-NP protein antibody together with an AF488-conjugated anti-rabbit IgG (Jackson ImmunoResearch, PA, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2021
Quote:
... and human anti-SARS-CoV-2 N mAb (N18) followed by IRDye® 680RD Goat anti-Human IgG Secondary Ab (LI-COR # 926-68078).
-
No products found
because this supplier's products are not listed.
Travis C. Glenn, et al.,
bioRxiv - Genomics 2019
Quote:
... coli genomic DNA to 400-600 bp using a Covaris S2 (Covaris, Woburn, MA, USA), and we fragmented genomic DNA (normalized to 23 ng/µL ...
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No products found
because this supplier's products are not listed.
Tobias Kletter, et al.,
bioRxiv - Systems Biology 2021
Quote:
R1/E mESCs were imaged on a Zeiss LSM 800 system (Carl Zeiss Microscopy, Jena, Germany) (sampling in xy ...
-
No products found
because this supplier's products are not listed.
Tao Wang, et al.,
bioRxiv - Neuroscience 2022
Quote:
... AggreWell 800 (STEMCELL Technologies) plates containing 300 microwells were used to generate uniformly sized spheroids ...