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Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction...
Cat# PBCA1047,
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Pragya D Yadav, et al.,
bioRxiv - Microbiology 2021
Quote:
... Pseudovirus (an HIV-based luciferase expressing lentivirus pseudotyped with SARS-CoV-2 full length S protein) was obtained from Creative Biogene. One step luciferase assay kit from BPS Bioscience was used for detection ...
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Mariève D. Boulanger, et al.,
bioRxiv - Bioengineering 2021
Quote:
... A volume of 100 μL of horseradish peroxidase (HRP)-conjugated IgY anti-protein G secondary antibody (HRP anti-protein G, OAIA00498, Aviva systems biology) solution (0.02 μg/mL of HRP anti-protein G diluted in rinsing solution with 1% BSA ...
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No products found
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Mustafa G. Aydogan, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2) The mCherry tag was replaced with either mNeonGreen (Shaner et al., 2013) (Allele Biotechnology) or mKate2 (Shcherbo et al. ...
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No products found
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Emily Ann McClure, et al.,
bioRxiv - Microbiology 2019
Quote:
... Sheep blood with heparin anticoagulant was purchased from Lampire Biological Laboratories (Pipersville ...
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No products found
because this supplier's products are not listed.
Mirjam C van der Net, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... was recloned in a pDONR221 backbone and the Myc-tag was replaced with a Flag-tag (Epoch Life Science). The sequence 5’-CAATGCGGAATATCAATCCCAGCACAGCAAATTCTCCA AAATGTCAGG-3’ was added between base pairs 982 and 983 of the YAP1 gene ...
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No products found
because this supplier's products are not listed.
Alexander B. Coley, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Human embryonic kidney (HEK293) cell line was obtained from GenLantis (San Diego, CA) and cultured in MEM (Mediatech ...
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No products found
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Satoshi Imanishi, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
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No products found
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Emma T Crooks, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by alkaline phosphatase labeled anti-human Fc conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT (Sigma).
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No products found
because this supplier's products are not listed.
Joseph R. Francica, et al.,
bioRxiv - Immunology 2021
Quote:
... and eosinophils by staining for eosinophil peroxidase (rabbit polyclonal, Atlas Antibodies). Inflammation was scored on the following scale based on % tissue affected ...
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No products found
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Deepti Ramachandran, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2) followed by development with the femtoLUCENT™ PLUS-HRP Chemiluminescent kit (G-Biosciences) for the anti-GDF3 blot and the SuperSignal™ West Pico Chemiluminescent kit (Thermo Fisher ...
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Histone Demethylase ( HDM ) Fluorescent assay Kit
Cat# K010-F1,
1.0 ea, USD $395.0
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Deyana Ivanova, et al.,
bioRxiv - Neuroscience 2022
Quote:
Blood samples were collected for CORT measurement were processed using a commercially available enzyme immunoassay (EIA) kit (sheep polyclonal antibody specific for corticosterone, AB_2877626; DetectX® Corticosterone Enzyme Immunoassay Kit, K014; Arbor Assays, Michigan, USA), as described previously (3).
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No products found
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Benjamin Orris, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 2-14C labeled 2’-deoxythymidine-5’-triphosphate (2-14C-dTTP) was obtained from Moravek biochemicals ...
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No products found
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Shirin Fatma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and 2’,2’-cGAMP were purchased from Axxora. All synthetic cyclic dinucleotides were further HPLC purified ...
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No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Stock solutions of recombinant Aβ peptides were prepared by solubilizing lyophilized monomers (Aβ38, rPeptide A-1078-2; Aβ40:rPeptide A-1153-2; and Aβ42: rPeptide A-1163-2) in 1% NH4OH at 200 µM concentration as recommended by manufacturer ...
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No products found
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Yuta Koganezawa, et al.,
bioRxiv - Genetics 2021
Quote:
... we spin-coated SU8-2 (MicroChem) on the wafer with the target height of 1.2 µm ...
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No products found
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Huiqiao Pan, et al.,
bioRxiv - Microbiology 2023
Quote:
... point style #2 needles (Hamilton Company) were used to inject 100 µL headspace samples into TRACE 1300/ISQ 7000 GC-MS equipped with a TracePLOT TG-BOND Q+ column (30 m x 0.32 mm x 10 µm ...
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No products found
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Lihong Chen, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... 2 mg monoclonal antibody to collagen II (Chondrex) in 50 ul of PBS was administered by intraperitoneal injection ...
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No products found
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Virginia Hargest, et al.,
bioRxiv - Microbiology 2019
Quote:
... homogenized using 2-mm zirconium oxide beads (Next Advance) beads for 4 minutes on speed setting 4 (Next Advance air cooling bullet blender) ...
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No products found
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Cato Prince, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and 2 mM MgCl2 and 200 U of mSAN nuclease (Arcticzymes catalog #70950-150). Cell lysates were incubated at 37°C for 1 hour ...
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No products found
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Tetsuro Yamamoto, et al.,
bioRxiv - Immunology 2023
Quote:
... horseradish peroxidase (HRP)-human IgG (EY Laboratories, Inc), and BT IgE antibodies (Bio-Rad Laboratories ...
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Cat# H2V220-1,
USD $1050.0/mg
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Akihisa Kato, et al.,
bioRxiv - Microbiology 2023
Quote:
... glycoprotein B (gB) (H1817; Virusys), Flag (M2 ...
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No products found
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Jacqueline M. Tokarew, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A 0.4 % horseradish peroxidase solution was prepared using HRP-linked anti-rabbit secondary antibody diluted in Stabilizyme solution (SurModics SZ02). Each read was set up in triplicate on a white polystyrene 96-well plate (ThermoFisher 236105 ...
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No products found
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Muttarin Lothong, et al.,
bioRxiv - Microbiology 2022
Quote:
Rabbit polyclonal antibody (pAb) against PRRSV envelop glycoprotein GP5 (Biorbyt Ltd., Cambridge, UK; dilution 1:100) was used to detect PRRSV protein ...
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No products found
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Fabian Braun, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Peroxidase was blocked (Ultra Vision Hydrogen Peroxidase Block, Medac-diagnostica, Germany) and a pretreatment with citrate buffer at pH6 followed (PT modul Buffer 1 ...
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No products found
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Kishor Dnyaneshwar Ingole, et al.,
bioRxiv - Plant Biology 2020
Quote:
... cDNAs of SUM1AA or SUM3AA were cloned as EcoRI-SalI fragment into pASK-IBA16 vector (N-terminal Strep-tag II affinity tag; Neuromics). For in vitro isoform influence on SUMOylation assays SUM1GG or SUM3GG were cloned as EcoRI-SalI fragment into pASK-IBA16 vector ...
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No products found
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Can Tan, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... cleared with FocusClear (CelExplorer Labs #FC-101) and mounted on slides in mounting medium.
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No products found
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Lauren A. Todd, et al.,
bioRxiv - Immunology 2021
Quote:
... an exogenous miRNA (cel-miR-39 Spike-In kit; Norgen Biotek Corp., Thorold, Canada) was spiked in (99 fmol ...
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No products found
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Silvia De Cicco, et al.,
bioRxiv - Neuroscience 2020
Quote:
NFAT Reporter - HEK293 Cell Line (PKC/ Ca2+ Pathway) cell lines were purchased from Tebu-Bio and cultured in Dulbecco’s MEM (Merck ...
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No products found
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Kanae Tsubotani, et al.,
bioRxiv - Biochemistry 2021
Quote:
... FITC-labelled lysozyme (LS1-FC-1, Nanocs Inc., USA) instead of lysozyme was mixed with ovalbumin in 5mM Tris buffer (pH 7.4) ...
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No products found
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Megha Rajendran, et al.,
bioRxiv - Biophysics 2022
Quote:
... FCS measurements were made in eight-well cover-glass slides (Grace Biolabs) pretreated with Sigmacoat (Sigma Aldrich ...
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No products found
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Robert M. Schilke, et al.,
bioRxiv - Immunology 2020
Quote:
... Data analysis was performed using FCS express (Denovo Software) and NovoExpress (Acea Biosciences).
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No products found
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Isaac L. Scott, et al.,
bioRxiv - Biophysics 2023
Quote:
... supplemented with 10% fetal calf serum (FCS) (Atlas Biologicals, Fort Collins, CO, USA) and 1x L-glutamine (Hyclone ...
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No products found
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Mareike Möller, et al.,
bioRxiv - Genetics 2020
Quote:
... Genomic DNA was fragmented with a COVARIS S2 and an Illumina-compatible library was prepared with the NEXTflex Bisulfite Library Prep Kit for Illumina Sequencing (Bioo Scientific/PerkinElmer, Austin, Texas, U.S.A). Bisulfite conversion was performed using the EZ DNA Methylation Gold Kit (Zymo Research ...
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No products found
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Rui Sun, et al.,
bioRxiv - Systems Biology 2022
Quote:
... AKT1-2 inhibitor MK-2206 (Targetmol, 1032350-13-2), and everolimus (Targetmol ...
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No products found
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Jessica T. Stieglitz, James A. Van Deventer,
bioRxiv - Synthetic Biology 2021
Quote:
... (S)-2-amino-6-((2-azidoethoxy)carbonylamino)hexanoic acid (Iris Biotech), (S)-2-amino-6-(((prop-2-yn-1-yloxy)carbonyl)amino)hexanoic acid (AstaTech) ...
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No products found
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Nicole M. Gaudelli, et al.,
bioRxiv - Genomics 2020
Quote:
... and 250IU/mL of recombinant human interleukin-2 (rhIL-2, CellGenix GmbH). Cells were activated with soluble human anti-CD3 (clone OKT3 ...
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No products found
because this supplier's products are not listed.
Ian W. McCahill, et al.,
bioRxiv - Plant Biology 2024
Quote:
... 2 media (Caisson Labs). Aqueous solutions of paclobutrazol and GA3 were freshly prepared and diluted to 0.1 µM and 10 µM respectively in hydroponic media ...
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No products found
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Pascale Lemieux, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... and 2% DMSO (Bioshop Canada)) to an OD of 0.1 ...
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No products found
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Katherine Williams, et al.,
bioRxiv - Genomics 2021
Quote:
... and 2% Ultrasor G (Crescent Chemical Co.). Day 0 cells were kept at low confluency to prevent spontaneous differentiation ...
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No products found
because this supplier's products are not listed.
Prabhu S. Arunachalam, et al.,
bioRxiv - Immunology 2021
Quote:
... Alum (Alhydrogel 2%) was purchased from Croda Healthcare (Batch #0001610348) ...
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No products found
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Gayani Wijegunawardena, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 2-chlorotrityl resin was purchased from Chempep Inc ...
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No products found
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Glenn F. W. Walpole, et al.,
bioRxiv - Cell Biology 2021
Quote:
... PtdIns(3,4)P2 (Echelon Biosciences P-3416-2); PtdIns(3,4,5)P3 (Echelon Biosciences P-3916-2) ...
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No products found
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Lina Freage, et al.,
bioRxiv - Biochemistry 2020
Quote:
... while OSJ-T3-OMe was synthesized by using the 2’-OMe-Ac-C-CE and 2’-OMe-U-CE phosphoramidites (Glen Research) to modify the 40th and 41st bases (Glen Research) ...
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No products found
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Rudolf O. Schlechter, et al.,
bioRxiv - Microbiology 2023
Quote:
... gas permeability of 0.6 m3 m−2 day−1 and water loss of 1 g m−2 day−1; Brooks Life Sciences, UK), and incubated at 30°C with shaking ...
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No products found
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Ting Zhu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 2 μl of Aβ1-42-555 was distributed into DG area of the hippocampus (AP −2 mm; ML −1.5 mm; DV 2 mm) using stereotaxic equipment (RWD Life Science Co., Ltd). After recovery from surgery ...
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No products found
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Alison C. Leonard, et al.,
bioRxiv - Biochemistry 2021
Quote:
... at 2000V using a 2 mm electroporation cuvette (Bulldog Bio) and Eppendorf electroporator and then plated on yeast extract peptone dextrose plus sorbitol plates (YPDS ...
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No products found
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Ziqiang Lin, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Sections were incubated in Histoclear (2×3 min; National Diagnostics, USA) and cover-slipped with mounting medium (Sigma Aldrich ...
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No products found
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Shengyun Ma, et al.,
bioRxiv - Immunology 2022
Quote:
... cells were cross-linked with 2 mM disuccinimidyl glutarate (DSG, ProteoChem) and 1% (vol/vol ...
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No products found
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Hope I Needs, et al.,
bioRxiv - Cell Biology 2022
Quote:
... cracked in a cell disruptor (Constant Systems; 2 cycles at 25 kpsi), and clarified by centrifugation (45 min ...
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No products found
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Anicca Harriot, et al.,
bioRxiv - Pathology 2023
Quote:
... Anesthetized mice (2-3% isoflurane) were placed in supine position on the temperature maintained (Deltaphase Isothermal Pad, Braintree Scientific) platform of an Aurora 3100 with the knee stabilized and foot affixed on the footplate of the torque transducer ...