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No products found
because this supplier's products are not listed.
Shota Yoshida, et al.,
bioRxiv - Immunology 2020
Quote:
... Synthetic SARS-CoV-2 peptides (Appendix Table S2; Peptide Institute Inc., Osaka, Japan) were diluted to a concentration of 10 μg/ml and coated at 500 ng/well ...
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No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... and SARS-CoV-2 smFISH probes were designed using Stellaris smFISH probe designer (Biosearch Technologies) available online at http://www.biosearchtech.com/stellaris-designer ...
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No products found
because this supplier's products are not listed.
Willemieke M. Kouwenhoven, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and finally for 3h with horseradish peroxidase streptavidin (016-030-084, Cedarlane, USA). The DAB reaction was carried out for 15s ...
-
No products found
because this supplier's products are not listed.
David Swygart, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Spike trains were recorded using glass pipettes (2–3MΩ) filled with AMES solution in cell-attached configuration ...
-
No products found
because this supplier's products are not listed.
Veronica Locher, et al.,
bioRxiv - Immunology 2022
Quote:
Cells were incubated in 2% FCS in PBS with 1 mM EDTA with human Fc block (STEMCELL Technologies) and stained with Aqua LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Erica Bello, et al.,
bioRxiv - Neuroscience 2023
Quote:
... coli Spike-in DNA (EpiCypher) was added to each sample to normalise reads to control for experimental variability and sequencing depth ...
-
No products found
because this supplier's products are not listed.
Prasanna Babu Araveti, et al.,
bioRxiv - Microbiology 2022
Quote:
... the fluorescence images of the HEK293 cells were captured using Axio Observer 7 microscope Apotome 2 (Carl Zeiss).
-
No products found
because this supplier's products are not listed.
Tianyi Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... day 0 and day 21) with 5 μg/dose of recombinant SARS-CoV-2 Spike protein (S1+S2 extracellular domain) (Bon Opus Biosciences, #BP040) adjuvanted with 100 μg/dose alhydrogel adjuvant 2% (InvivoGen ...
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No products found
because this supplier's products are not listed.
Christopher Cyrus Kuhn, et al.,
bioRxiv - Cell Biology 2022
Quote:
SARS-CoV-2 S protein (Cube Biotech) was recorded at 0.3 mg/ml ...
-
No products found
because this supplier's products are not listed.
Sangam Kandel, et al.,
bioRxiv - Genomics 2023
Quote:
... Samples were tested for the SARS-CoV-2 using the Aptima® SARS-CoV-2 (Panther® System, Hologic, San Diego, CA) nucleic acid amplification assay ...
-
No products found
because this supplier's products are not listed.
Huanzhou Xu, et al.,
bioRxiv - Microbiology 2020
Quote:
... rabbit anti-SARS-CoV-2 ORF3a antibody (FabGennix, Cat. SARS-COV2-ORF3A-101AP), HRP-conjugated goat anti-mouse IgG(H+L ...
-
No products found
because this supplier's products are not listed.
Chamandi S. Dampalla, et al.,
bioRxiv - Microbiology 2021
Quote:
... SARS-CoV-2 3CLpro complex with compound 2: Index HT screen (Rigaku Reagents) condition G8 (25% (w/v ...
-
No products found
because this supplier's products are not listed.
Dalia Raïch-Regué, et al.,
bioRxiv - Microbiology 2022
Quote:
... The amount of SARS-CoV-2 nucleoprotein released to the supernatant was measured with SARS-CoV-2 nucleocapsid protein High-Sensitivity Quantitative ELISA (ImmunoDiagnostics) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Chamandi S. Dampalla, et al.,
bioRxiv - Microbiology 2021
Quote:
... SARS-CoV 3CLpro complex with compound 2: Index HT screen (Hampton Research) condition H4 (30% (w/v ...
-
Magnetofection
diificult to transfect cells
Cat# KC30396,
SilenceMag 200µL + PolyMag 100µL+ PolyMag Neo 100µL + Magnetic Plate MF96000, USD $546.00/KIT
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Shima Shahbaz, et al.,
bioRxiv - Immunology 2020
Quote:
SARS-CoV-2 stocks were pre-incubated with ViroMag transduction reagent (OZ Biosciences) as reported elsewhere31–37,38 ...
-
No products found
because this supplier's products are not listed.
Sebastian Fiedler, et al.,
bioRxiv - Biochemistry 2020
Quote:
Anti-SARS-CoV-2 seropositive human serum samples (convalescent) were obtained from BioIVT. BioIVT sought informed consent from each subject ...
-
No products found
because this supplier's products are not listed.
Mohamed Fareh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Kristin Metzdorf, et al.,
bioRxiv - Immunology 2022
Quote:
... 3 μm thick FFPE sections were stained for SARS-CoV-2 with mouse-anti-nucleocapsid CoV-1/2 (Synaptic Systems, HS-452 11, clone 53E2, subtype: IgG2a) and for macrophages with rat-anti-mouse-MAC2 (Biozol Diagnostica/CEDARLANE,CL8942AP ...
-
No products found
because this supplier's products are not listed.
Ross N. England, et al.,
bioRxiv - Immunology 2023
Quote:
... diproline-modified spike protein from the SARS-CoV-2 Wuhan-Hu-1 strain were produced as previously described15,16 with m1Ψ-5-triphosphate (TriLink) instead of UTP and capped cotranscriptionally using the trinucleotide cap1 analog ...
-
No products found
because this supplier's products are not listed.
Anastasia Polycarpou, et al.,
bioRxiv - Immunology 2023
Quote:
UV inactivation of virus was performed by exposure of SARS-CoV-2 (England 02/2020) at a maximal depth of 1.5mm in 6-well plates (Sarstedt) placed on ice ...
-
No products found
because this supplier's products are not listed.
J. Rafael Ciges-Tomas, et al.,
bioRxiv - Biophysics 2022
Quote:
The synthetic gene for the SARS-CoV-2 NCTD (residues 256 to 364) cloned into the vector pET28a(+) was provided by Biomatik. The insert is in frame with the N-terminal His6-tag ...
-
No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
-
No products found
because this supplier's products are not listed.
Ghada Elfayres, et al.,
bioRxiv - Microbiology 2023
Quote:
HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
-
No products found
because this supplier's products are not listed.
Yufei Xiang, et al.,
bioRxiv - Bioengineering 2020
Quote:
The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Robert V. Blair, et al.,
bioRxiv - Pathology 2020
Quote:
Samples collected at preinfection and weekly post-infection until necropsy were tested for detection of binding IgG antibodies against SARS-CoV-2 nucleoprotein (NP) by MFIA COVID-Plex from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
Irina A. Favorskaya, et al.,
bioRxiv - Immunology 2021
Quote:
The RBD nucleotide sequence of SARS-CoV-2 Wuhan-Hu-1 isolate (Genbank accession number MN908947, from 319 to 545 aa) was synthesized (Evrogen, Russia) and cloned into the pCEP4 mammalian expression vector (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Diego Y. Grinman, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or horseradish peroxidase (HRP) anti-rabbit (RMR 622-G, Biocare) and developed with 3,3’-diaminobenzidine (DAB ...
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No products found
because this supplier's products are not listed.
Winston Chiu, et al.,
bioRxiv - Immunology 2022
Quote:
... the plates were transferred to the BSL-3+ Caps-It robotics system where 30 µL SARS-CoV-2 was added using a noncontact liquid handling system (Tecan, EVO 100) to achieve a multiplicity of infection (MOI ...
-
No products found
because this supplier's products are not listed.
Rodney G King, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
-
No products found
because this supplier's products are not listed.
Elizabeth R. Vanderwall, et al.,
bioRxiv - Immunology 2021
Quote:
... To measure SARS-CoV-2 replication in AEC cultures we used the Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit (Primerdesign®), with duplicate assays of harvested RNA from each SARS-CoV-2-infected AEC experimental condition ...
-
No products found
because this supplier's products are not listed.
DaoFei Song, Lei Yin, Chang Wang, XiuYing Wen,
bioRxiv - Molecular Biology 2019
Quote:
... Horseradish peroxidase-conjugated goat anti-rabbit IgG was purchased from Bioworld Technology ...
-
No products found
because this supplier's products are not listed.
Nicholas You Zhi Cheang, et al.,
bioRxiv - Immunology 2023
Quote:
... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Haijing Guo, Jen-Hsuan Wei, Joachim Seemann,
bioRxiv - Cell Biology 2020
Quote:
... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
-
No products found
because this supplier's products are not listed.
Reshmi Mukherjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Synthetic 2′-FL (s2′-FL) was purchased from Carbosynth (www.carbosynth.com). The c2′-FL contains 94.1 % 2′-FL ...
-
No products found
because this supplier's products are not listed.
Oscar J. Ortiz-Arrazola, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Anti-IgG (rabbit conjugated to HRP (horseradish peroxidase) (Enzo Life Sciences, Inc., Farmingdale, USA). USA ...
-
No products found
because this supplier's products are not listed.
Robert W. Cross, et al.,
bioRxiv - Microbiology 2020
Quote:
... Wells then were incubated for 1 hour with goat anti-human IgG conjugated to horseradish peroxidase (Fitzgerald Industries International) at a 1:20,000 dilution ...
-
No products found
because this supplier's products are not listed.
Audra R. Fullen, et al.,
bioRxiv - Microbiology 2022
Quote:
... the plates were incubated with wheat germ agglutinin (WGA) conjugated to horseradish peroxidase (HRP) (Biotium Inc. Hayward, CA, USA) at a dilution of 1:1000 ...
-
No products found
because this supplier's products are not listed.
Denise Pajonczyk, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
ERK1/2 phosphorylation in HEK293 FPR cell systems was measured with the HTRF immunoassay from Cisbio, as described previously [37] ...
-
No products found
because this supplier's products are not listed.
James R. Byrnes, et al.,
bioRxiv - Immunology 2021
Quote:
... beads were collected and glycoproteins reduced with 5 mM TCEP (Calbiochem) for 30 minutes at 37°C and alkylated with 11 mM iodoacetamide (Sigma ...
-
No products found
because this supplier's products are not listed.
Navid Paknejad, Vinay Sapuru, Richard K. Hite,
bioRxiv - Biophysics 2022
Quote:
... Membrane proteins were solubilized from 2.4 L of pelleted HEK293S GnTI-cells expressing wild-type hIP3R3 for 2 hours by rotation in 2% lauryl maltose neopentyl glycol (LMNG; Anatrace Cat# NG310), 150 mM sodium chloride (NaCl) ...
-
No products found
because this supplier's products are not listed.
Till Stephan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Spot tag (ChromoTek, Planegg-Martinsried, Germany), TOMM22 (Merck ...
-
No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 10% FCS (PromoCell, Germany). HEK-NF-κB_luc were supplemented for routine growth with 50 µg/mL Hygromycin B (Invivogen ...
-
No products found
because this supplier's products are not listed.
Valentina Fajner, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... fly ovaries were dissected in Drosophila S2 medium with 10 % of FBS (Euroclone) and incubated with 2.5% 1,6-hexanediol on an orbital shaker at room temperature for 5 minutes (Fig ...
-
No products found
because this supplier's products are not listed.
Abdulbasit Amin, et al.,
bioRxiv - Physiology 2022
Quote:
... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
-
No products found
because this supplier's products are not listed.
Ashley A. Johnson, et al.,
bioRxiv - Physiology 2021
Quote:
... Spectra were measured from HEK293 cells using a 60x objective with NA 1.45 (Nikon) and an inverted microscope (Nikon TE-2000) ...
-
No products found
because this supplier's products are not listed.
Siyi Gu, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK293 cells expressing Flag-SNAP-CCR5 were seeded onto 10-mm glass-bottom dishes (FluoroDish, FD3510, WPI) previously coated with 5 μg/ml fibronectin ...
-
No products found
because this supplier's products are not listed.
Binayak Sarkar, et al.,
bioRxiv - Microbiology 2024
Quote:
... while DAGs/TAGs were resolved using a Luna 5U C-5 column (Phenomenex) using established solvent systems.
-
No products found
because this supplier's products are not listed.
Jaigeeth Deveryshetty, et al.,
bioRxiv - Biochemistry 2019
Quote:
Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
-
No products found
because this supplier's products are not listed.
Alba Ventos-Alfonso, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... the bisulfite conversion ratio was calculated using spike-in controls provided by Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode) (supplementary Table S2 ...
-
No products found
because this supplier's products are not listed.
Sebastian Tacke, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plain grids (Quantifoil 2/2) were transferred to the microscope ...