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No products found
because this supplier's products are not listed.
Paul-Henri Romeo, et al.,
bioRxiv - Cell Biology 2023
Quote:
... SARS-CoV-2 Spike antibody (Active Motif, clone AM001414) was used for neutralization at 10nM.
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No products found
because this supplier's products are not listed.
Edward Sullivan, et al.,
bioRxiv - Microbiology 2021
Quote:
... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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No products found
because this supplier's products are not listed.
Shiho Tanaka, et al.,
bioRxiv - Immunology 2021
Quote:
... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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No products found
because this supplier's products are not listed.
Lok Man John Law, et al.,
bioRxiv - Microbiology 2021
Quote:
CHO cells stably expressing recombinant RBD of SARS-CoV-2 Spike (aa. 319-591) (GenBank accession no. AAP13567.1) were propagated in ProCho5 media (Lonza) containing glutamine ...
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No products found
because this supplier's products are not listed.
Lieve E.H. van der Donk, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV-2 pseudovirus productions were quantified by p24 ELISA (Perkin Elmer Life Sciences).
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No products found
because this supplier's products are not listed.
Akiko Yoshihara, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Goat anti-rabbit IgG antibody conjugated with horseradish peroxidase (SA00001-2, Proteintech) was secondarily reacted with the anti-LPOR antibody and detected using a chemiluminescence reagent (Immobilon ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
Georg OM Bobkov, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Secondary antibodies coupled to horseradish peroxidase (Dianova) were used at 1:10000.
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No products found
because this supplier's products are not listed.
B Balakrishnan, K Lai,
bioRxiv - Biochemistry 2021
Quote:
... anti-SARS-CoV-2 Spike Protein was purchased from Origene Technologies Inc ...
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No products found
because this supplier's products are not listed.
Brett Stern, Peter Monteleone, Janet Zoldan,
bioRxiv - Bioengineering 2022
Quote:
Viral spike protein (either SARS-CoV-2 Spike Protein, S1 Subunit, RayBiotech, or MERS-CoV-1 Spike Protein, S1 Subunit, BioVision) was added to endothelial culture media of CD34+ iPSC-EP-laden hydrogels at a concentration of 10 μg/mL either one or five days after cell encapsulation ...
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No products found
because this supplier's products are not listed.
Annalisa Tito, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were then infected with SARS-CoV-2 Spike-Pseudotyped Lentivirus (Firefly Luciferase SARS-CoV-2 lentiviral particles-GeneCopoeia) and the control VSV-G protein pseudotyped Lentivirus (HLUC-Lv201 Firefly luciferase − eGFP lentifect-GeneCopoeia ...
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No products found
because this supplier's products are not listed.
Youjia Guo, et al.,
bioRxiv - Microbiology 2020
Quote:
... followed by incubation with horseradish peroxidase (HRP) conjugated sheep anti-mouse secondary antibody (MP Biomedicals) 1:5000 diluted in blocking buffer for 30 min at room temperature ...
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No products found
because this supplier's products are not listed.
Breanne E. Haskins, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-horseradish peroxidase (BioXCell) prior to and after infection on various days ...
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No products found
because this supplier's products are not listed.
Romain Rouet, et al.,
bioRxiv - Immunology 2020
Quote:
... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Vania Passos, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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No products found
because this supplier's products are not listed.
Cristina Marí-Carmona, et al.,
bioRxiv - Plant Biology 2021
Quote:
Horseradish peroxidase-conjugated anti-rabbit (Agrisera) and anti-mouse (Agrisera ...
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No products found
because this supplier's products are not listed.
Chenjian Gu, et al.,
bioRxiv - Microbiology 2020
Quote:
... pCAGGS-SARS-CoV-2-S that encodes the SARS-CoV-2 Spike gene was generated by GENEWIZ (Suzhou, China). Recombinant adenovirus expressing human ACE2 (Ad5-hACE2 ...
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No products found
because this supplier's products are not listed.
Hoai Thi Thu Tran, et al.,
bioRxiv - Microbiology 2022
Quote:
SARS-CoV-2 Spike B.1.1.529 (Omicron) variant pseudotyped lentivirus particles were purchased from Biomol (Hamburg, Germany). The transduction assay was performed as described previously [12] ...
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No products found
because this supplier's products are not listed.
Niayesh Razi, et al.,
bioRxiv - Microbiology 2023
Quote:
The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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No products found
because this supplier's products are not listed.
Bennett J. Davenport, et al.,
bioRxiv - Immunology 2021
Quote:
... and probed with 1 μg/mL of chimeric human anti-SARS-CoV spike antibody (CR3022, Absolute Antibody, Ab01680) in Perm Wash (1X PBS/0.1% saponin/0.1% BSA ...
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No products found
because this supplier's products are not listed.
Cong Xu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... biotinylated SARS-CoV-2 S trimer protein was loaded onto streptavidin (SA) biosensors (Pall FortéBio). S-trimer-bound biosensors were dipped into wells containing varying concentrations of ACE2 protein and the interactions were monitored over a 500-sec association period ...
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No products found
because this supplier's products are not listed.
Krishna Gupta, Elisha Krieg,
bioRxiv - Synthetic Biology 2024
Quote:
... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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No products found
because this supplier's products are not listed.
Zixuan Yuan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Horseradish peroxidase and choline oxidase were purchased from VWR. Methylbetacyclodextrin (MβCD ...
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SARS-CoV-2 Spike Glycoprotein is a recombinant protein expressed in Mammalian cells.
Cat# abx620076-50UG,
50 µg USD $333.5
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Bálint Kiss, et al.,
bioRxiv - Biophysics 2020
Quote:
... 100 μl of 10 μg/ml SARS-CoV-2 Spike Glycoprotein Antibody (#abx376478, Abbexa Ltd, Cambridge, UK) was then added ...
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No products found
because this supplier's products are not listed.
Kevin W. Ng, et al.,
bioRxiv - Immunology 2021
Quote:
... mice were injected intraperitoneally with 25 µg recombinant SARS-CoV-2 S2 (CV2006; LifeSensors) in monophosphoryl lipid A adjuvant (Sigma Adjuvant System ...
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No products found
because this supplier's products are not listed.
Nataly Stylianou, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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No products found
because this supplier's products are not listed.
Mariana F. Tioni, et al.,
bioRxiv - Immunology 2021
Quote:
Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
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No products found
because this supplier's products are not listed.
Tanay Bhatt, et al.,
bioRxiv - Pathology 2022
Quote:
About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
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No products found
because this supplier's products are not listed.
Hang Ma, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the binding of antibodies with SARS-CoV-2 antigens were visualized by adding 100 μL peroxidase substrate TMB Single-Component Substrate solution (Solarbio) and incubating for 15 min in dark ...
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No products found
because this supplier's products are not listed.
Kyung Ku Jang, et al.,
bioRxiv - Cell Biology 2022
Quote:
SARS-CoV-2-infected monolayers were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 3 hrs at RT ...
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No products found
Lisa M Mehlmann, et al.,
bioRxiv - Cell Biology 2019
Quote:
... or WesternBright Sirius horseradish peroxidase substrate (Advansta), using a charge-coupled device camera (G:box Chemi XT4 ...
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No products found
because this supplier's products are not listed.
Liguo Zhang, et al.,
bioRxiv - Genomics 2020
Quote:
Cells subject to SARS-CoV-2 infection were grown in μ-Slide 8 Well (#1.5 polymer, Ibidi 80826) and fixed with 4% paraformaldehyde/CMF-PBS at room temperature (RT ...
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No products found
because this supplier's products are not listed.
Denise Haslwanter, et al.,
bioRxiv - Microbiology 2021
Quote:
Competitive binding of mAbs to recombinant SARS-CoV-2 RBD with hACE2 was evaluated using the ForteBio Octet HTX instrument (Molecular Devices) as described previously (34) ...
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No products found
because this supplier's products are not listed.
Arkaprabha Basu, et al.,
bioRxiv - Biophysics 2021
Quote:
... The membranes were then incubated in TBST containing 5% BSA for 1 hour with horseradish peroxidase-conjugated goat anti-mouse IgG and horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (LI-COR Biosciences, Lincoln, NE). After that ...
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No products found
because this supplier's products are not listed.
Yan Wang, Meiling Lian, Liping Song, Shengzhou Wu,
bioRxiv - Neuroscience 2020
Quote:
... goat anti-Rabbit horseradish peroxidase conjugated IgG(Boster Biological Technology). The following reagents or cell lines were used in this study:the Dicer1 siRNA duplex and the negative control(NC ...
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No products found
because this supplier's products are not listed.
Hui-Hsuan Kuo, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ...
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No products found
because this supplier's products are not listed.
Xinhai Ye, et al.,
bioRxiv - Genomics 2020
Quote:
... the DNA firstly sheared by Covaris S2 system (Covaris, Inc. Woburn, MA, USA), then DNA fragments were end polished ...
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No products found
because this supplier's products are not listed.
Christopher J. Emig, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... SARS-CoV-2 Delta Variant pseudovirus (eEnzyme) was diluted 1:2 in DMEM complete media to a pseudoviral particle concentration of 5e7/ml ...
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No products found
because this supplier's products are not listed.
Guillermo Valenzuela Nieto, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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No products found
because this supplier's products are not listed.
Alexandria N. Miller, et al.,
bioRxiv - Biophysics 2022
Quote:
... purified SARS-CoV-2 Orf3a was reconstituted in soybean polar lipid extract (Avanti Polar Lipids) at 1:10 and 1:100 wt:wt protein to lipid ratios ...
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No products found
because this supplier's products are not listed.
Bin Zhou, et al.,
bioRxiv - Microbiology 2020
Quote:
... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
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A soluble, dialyzed, lyophilized powder. RZ ≥1.0.
Cat# LS002561,
Bulk, Inquire
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M. Alejandra Tortorici, et al.,
bioRxiv - Immunology 2021
Quote:
... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
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No products found
because this supplier's products are not listed.
Mehdi Moustaqil, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... coli C41(DE3) cells were transformed with the SARS-CoV-2 pET-28a-nsp5 plasmid (IDT DNA). Single colonies were used to inoculate LB media supplemented with kanamycin (50 μg/mL) ...
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No products found
because this supplier's products are not listed.
Luiz Gustavo Teixeira Alves, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2 isolate was grown on Vero E6 cells in minimal essential medium (MEM; PAN Biotech) with 10% fetal bovine serum (PAN Biotech ...
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No products found
because this supplier's products are not listed.
Anna C. Fagre, et al.,
bioRxiv - Microbiology 2020
Quote:
... Horizontal whole-lung slides of consecutive planes of SARS-CoV-2-infected and antibody-treated lungs were digitalized by Olympus histoslide scanner to create image data files that can be used for further digital image analysis (DIA) ...
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No products found
because this supplier's products are not listed.
Bert Schepens, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... spiked controls and diluted samples were mixed with 3 nM VHH72-h1 (S56A)-Flag3-His3 and 2.5 nM biotinylated SARS-CoV-2 RBD protein in white low binding 384-well microtitre plates (F-bottom, Greiner Cat nr 781904). After an incubation for 1 hour at room temperature ...
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No products found
because this supplier's products are not listed.
Eriko Ohgitani, et al.,
bioRxiv - Microbiology 2020
Quote:
Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.
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No products found
because this supplier's products are not listed.
Juan C. Burckhardt, et al.,
bioRxiv - Microbiology 2022
Quote:
... defibrinated sheep blood (Hemostat Laboratories) was added to the media at a final volume of 5% (v/v) ...
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No products found
because this supplier's products are not listed.
Mykhailo Y. Batiuk, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 2 were prepared using FCS Express 7 Plus v7.04.0014 (De Novo Software).