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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
Lieve E.H. van der Donk, et al.,
bioRxiv - Immunology 2021
Quote:
... SARS-CoV-2 pseudovirus productions were quantified by p24 ELISA (Perkin Elmer Life Sciences).
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No products found
because this supplier's products are not listed.
Vania Passos, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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No products found
because this supplier's products are not listed.
Chenjian Gu, et al.,
bioRxiv - Microbiology 2020
Quote:
... pCAGGS-SARS-CoV-2-S that encodes the SARS-CoV-2 Spike gene was generated by GENEWIZ (Suzhou, China). Recombinant adenovirus expressing human ACE2 (Ad5-hACE2 ...
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No products found
because this supplier's products are not listed.
B Balakrishnan, K Lai,
bioRxiv - Biochemistry 2021
Quote:
... anti-SARS-CoV-2 Spike Protein was purchased from Origene Technologies Inc ...
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No products found
because this supplier's products are not listed.
Annalisa Tito, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were then infected with SARS-CoV-2 Spike-Pseudotyped Lentivirus (Firefly Luciferase SARS-CoV-2 lentiviral particles-GeneCopoeia) and the control VSV-G protein pseudotyped Lentivirus (HLUC-Lv201 Firefly luciferase − eGFP lentifect-GeneCopoeia ...
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No products found
because this supplier's products are not listed.
Guillermo Valenzuela Nieto, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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No products found
because this supplier's products are not listed.
Stéphanie Thébault, et al.,
bioRxiv - Microbiology 2022
Quote:
... Biotinylated SARS-CoV-2 RBD or S1 (4 µg/mL) were loaded on streptavidin SA (18-0009) biosensors (Pall ForteBio) for 1 min ...
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No products found
because this supplier's products are not listed.
Tanay Bhatt, et al.,
bioRxiv - Pathology 2022
Quote:
About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
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No products found
because this supplier's products are not listed.
Romain Rouet, et al.,
bioRxiv - Immunology 2020
Quote:
... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Biao Zhou, et al.,
bioRxiv - Microbiology 2021
Quote:
... immunofluorescence staining was performed on deparaffinized and rehydrated tissue sections using a rabbit anti-SARS-CoV-2-NP protein antibody together with an AF488-conjugated anti-rabbit IgG (Jackson ImmunoResearch, PA, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Maud Dupont, et al.,
bioRxiv - Microbiology 2023
Quote:
... HA-tag (Proteintech, 66006-2-ig), Gaussia luciferase (New England Biolabs ...
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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2/COVID-19/2019-nCoV) Spike...
Cat# abx600065-100UG,
100 µg USD $826.5
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Bálint Kiss, et al.,
bioRxiv - Biophysics 2020
Quote:
... 100 μl of 10 μg/ml SARS-CoV-2 Spike Glycoprotein Antibody (#abx376478, Abbexa Ltd, Cambridge, UK) was then added ...
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
... Human IgM anti-SARS-CoV-2 Spike (S1) Antibody CR3022 (Native Antigen, Oxford, UK) and anti-Spike RBD (SARS-CoV-2/COVID 19) human monoclonal antibody (eEnzyme, Gaithersburg, USA) were used as positive controls in ELISA ...
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No products found
because this supplier's products are not listed.
Hoai Thi Thu Tran, et al.,
bioRxiv - Microbiology 2022
Quote:
SARS-CoV-2 Spike B.1.1.529 (Omicron) variant pseudotyped lentivirus particles were purchased from Biomol (Hamburg, Germany). The transduction assay was performed as described previously [12] ...
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No products found
because this supplier's products are not listed.
Niayesh Razi, et al.,
bioRxiv - Microbiology 2023
Quote:
The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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No products found
because this supplier's products are not listed.
Wael Bahnan, et al.,
bioRxiv - Immunology 2021
Quote:
... making them permissive to SARS-CoV-2 infection (Jackson laboratories). One day after infection ...
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No products found
because this supplier's products are not listed.
Gabriela Mattoso Coelho, et al.,
bioRxiv - Microbiology 2023
Quote:
... washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000) (MP Biomedicals, Irvine, CA, USA) or the in-house anti-SARS-CoV-2 mAb (1/400) ...
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No products found
because this supplier's products are not listed.
Bennett J. Davenport, et al.,
bioRxiv - Immunology 2021
Quote:
... and probed with 1 μg/mL of chimeric human anti-SARS-CoV spike antibody (CR3022, Absolute Antibody, Ab01680) in Perm Wash (1X PBS/0.1% saponin/0.1% BSA ...
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No products found
because this supplier's products are not listed.
Kyung Ku Jang, et al.,
bioRxiv - Cell Biology 2022
Quote:
SARS-CoV-2-infected monolayers were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 3 hrs at RT ...
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No products found
because this supplier's products are not listed.
Emanuele Andreano, et al.,
bioRxiv - Immunology 2021
Quote:
... NTD or S2 SARS-CoV-2 protein diluted in carbonate-bicarbonate buffer (E107, Bethyl laboratories) were coated in 384-well plates (microplate clear ...
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No products found
because this supplier's products are not listed.
Alexandria N. Miller, et al.,
bioRxiv - Biophysics 2022
Quote:
... purified SARS-CoV-2 Orf3a was reconstituted in soybean polar lipid extract (Avanti Polar Lipids) at 1:10 and 1:100 wt:wt protein to lipid ratios ...
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No products found
because this supplier's products are not listed.
Krishna Gupta, Elisha Krieg,
bioRxiv - Synthetic Biology 2024
Quote:
... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
-
No products found
because this supplier's products are not listed.
Liguo Zhang, et al.,
bioRxiv - Genomics 2020
Quote:
Cells subject to SARS-CoV-2 infection were grown in μ-Slide 8 Well (#1.5 polymer, Ibidi 80826) and fixed with 4% paraformaldehyde/CMF-PBS at room temperature (RT ...
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No products found
because this supplier's products are not listed.
Meenakshi Kar, et al.,
bioRxiv - Immunology 2024
Quote:
... The impact of CD8+ T cell depletions on SARS-CoV-2 infection was also evaluated using anti-CD8β (clone 53-5.8, BioXCell) and isotype mAb IgG2b (clone LTF-2 ...
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No products found
because this supplier's products are not listed.
Denise Haslwanter, et al.,
bioRxiv - Microbiology 2021
Quote:
Competitive binding of mAbs to recombinant SARS-CoV-2 RBD with hACE2 was evaluated using the ForteBio Octet HTX instrument (Molecular Devices) as described previously (34) ...
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No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2021
Quote:
... and human anti-SARS-CoV-2 N mAb (N18) followed by IRDye® 680RD Goat anti-Human IgG Secondary Ab (LI-COR # 926-68078).
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No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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No products found
because this supplier's products are not listed.
Jesus Silvas, et al.,
bioRxiv - Microbiology 2021
Quote:
... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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No products found
because this supplier's products are not listed.
Hui-Hsuan Kuo, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and SARS-CoV-2 Spike protein S1 (Icosagen OÜ, Estonia, cat# P-305-100) were used in this study.
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No products found
because this supplier's products are not listed.
Nataly Stylianou, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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No products found
because this supplier's products are not listed.
Mariana F. Tioni, et al.,
bioRxiv - Immunology 2021
Quote:
Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
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No products found
because this supplier's products are not listed.
Bin Zhou, et al.,
bioRxiv - Microbiology 2020
Quote:
... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
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Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity...
Cat# LS04070,
10 ku, $56.00
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M. Alejandra Tortorici, et al.,
bioRxiv - Immunology 2021
Quote:
... MLV-SARS-CoV-2 pseudovirus was activated with 10 µg/ml TPCK treated-Trypsin (Worthington Biochem) for 1 h at 37 °C ...
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No products found
because this supplier's products are not listed.
Mehdi Moustaqil, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... coli C41(DE3) cells were transformed with the SARS-CoV-2 pET-28a-nsp5 plasmid (IDT DNA). Single colonies were used to inoculate LB media supplemented with kanamycin (50 μg/mL) ...
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No products found
because this supplier's products are not listed.
Luiz Gustavo Teixeira Alves, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2 isolate was grown on Vero E6 cells in minimal essential medium (MEM; PAN Biotech) with 10% fetal bovine serum (PAN Biotech ...
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No products found
because this supplier's products are not listed.
Anna C. Fagre, et al.,
bioRxiv - Microbiology 2020
Quote:
... Horizontal whole-lung slides of consecutive planes of SARS-CoV-2-infected and antibody-treated lungs were digitalized by Olympus histoslide scanner to create image data files that can be used for further digital image analysis (DIA) ...
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No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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No products found
because this supplier's products are not listed.
Bert Schepens, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... spiked controls and diluted samples were mixed with 3 nM VHH72-h1 (S56A)-Flag3-His3 and 2.5 nM biotinylated SARS-CoV-2 RBD protein in white low binding 384-well microtitre plates (F-bottom, Greiner Cat nr 781904). After an incubation for 1 hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Eriko Ohgitani, et al.,
bioRxiv - Microbiology 2020
Quote:
Neutralizing assay was performed using SARS-CoV-2 Neutralizing Antibody Detection ELISA Kit (Cayman Chemical, Ann Arbor, MI, USA; Item No. 502070) according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
David Swygart, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Spike trains were recorded using glass pipettes (2–3MΩ) filled with AMES solution in cell-attached configuration ...
-
No products found
because this supplier's products are not listed.
Matthew L. Goodwin, et al.,
bioRxiv - Immunology 2020
Quote:
... CMV glycoprotein–specific antibody binding was detected with phycoerythrin-conjugated goat anti-human IgG (2 μg/mL, Southern Biotech). Beads were washed and acquired on a Bio-Plex 200 instrument (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Richard A. Guyer, et al.,
bioRxiv - Neuroscience 2022
Quote:
... supplemented with 1% N2 supplement and 2% NeuroCult S1 supplement (STEMCELL Technologies, Vancouver, BC). After one day media was removed and replaced with a thin layer of phosphate buffered saline ...
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No products found
because this supplier's products are not listed.
Juan C. Burckhardt, et al.,
bioRxiv - Microbiology 2022
Quote:
... defibrinated sheep blood (Hemostat Laboratories) was added to the media at a final volume of 5% (v/v) ...
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No products found
because this supplier's products are not listed.
Mykhailo Y. Batiuk, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 2 were prepared using FCS Express 7 Plus v7.04.0014 (De Novo Software).
-
No products found
because this supplier's products are not listed.
Prasanna Babu Araveti, et al.,
bioRxiv - Microbiology 2022
Quote:
... the fluorescence images of the HEK293 cells were captured using Axio Observer 7 microscope Apotome 2 (Carl Zeiss).
-
No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... 10% of heat-inactivated fetal calf serum (FCS; Pan-Biotek, Aidenbach, Germany), supplemented with 50 ng/ml macrophage colony stimulating factor (M-CSF ...
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No products found
because this supplier's products are not listed.
Sebastian Tacke, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... plain grids (Quantifoil 2/2) were transferred to the microscope ...