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No products found
because this supplier's products are not listed.
Tianyi Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... day 0 and day 21) with 5 μg/dose of recombinant SARS-CoV-2 Spike protein (S1+S2 extracellular domain) (Bon Opus Biosciences, #BP040) adjuvanted with 100 μg/dose alhydrogel adjuvant 2% (InvivoGen ...
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No products found
because this supplier's products are not listed.
Chengjin Ye, et al.,
bioRxiv - Microbiology 2022
Quote:
... and SARS-CoV-2 M protein was purchased from ProteoGenix (Catalog# PX-COV-P025).
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No products found
because this supplier's products are not listed.
Anne-Catherine Fluckiger, et al.,
bioRxiv - Immunology 2021
Quote:
The expression of SARS-CoV-2 S protein in eVLP preparations was analyzed by western blotting as described previously [18] using rabbit polyclonal Ab (pAb) anti-spike protein of SARS-CoV-2 (Immune Technology Corp) followed by detection with goat anti-rabbit IgG-Fc horseradish peroxydase-conjugated (Bethyl) ...
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No products found
because this supplier's products are not listed.
Camilla Margaroli, et al.,
bioRxiv - Pathology 2022
Quote:
... Anti-SARS-CoV-2 was conjugated to PE / R-Phycoerythrin (Expedeon Lightning-Link R-PE Conjugation Kit / Abcam ...
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No products found
because this supplier's products are not listed.
Nicholas You Zhi Cheang, et al.,
bioRxiv - Immunology 2023
Quote:
... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
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Magnetofection
diificult to transfect cells
Cat# KC30396,
SilenceMag 200µL + PolyMag 100µL+ PolyMag Neo 100µL + Magnetic Plate MF96000, USD $546.00/KIT
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Shima Shahbaz, et al.,
bioRxiv - Immunology 2020
Quote:
SARS-CoV-2 stocks were pre-incubated with ViroMag transduction reagent (OZ Biosciences) as reported elsewhere31–37,38 ...
-
No products found
because this supplier's products are not listed.
Daniela Bottero, et al.,
bioRxiv - Immunology 2023
Quote:
... LightMix® Modular SARS-CoV (COVID19) RdRp (TIB MOLBIOL), and RNAsa P following the protocol developed by Dr ...
-
No products found
because this supplier's products are not listed.
Mesfin Meshesha, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and SARS-CoV-2 lineage B.1.1.529 (Omicron Variant) culture fluid (UV inactivated, 0810642UV, Zeptometrix LLC, USA) and (heat inactivated ...
-
No products found
because this supplier's products are not listed.
Raphael J. Eberle, et al.,
bioRxiv - Biochemistry 2021
Quote:
SARS-CoV-2 3CLpro activity assay was performed using a fluorogenic substrate DABCYL- KTSAVLQ↓SGFRKME-EDANS (Bachem, Switzerland) in a buffer containing 20 mM Tris pH 7.2 ...
-
No products found
because this supplier's products are not listed.
J. Rafael Ciges-Tomas, et al.,
bioRxiv - Biophysics 2022
Quote:
The synthetic gene for the SARS-CoV-2 NCTD (residues 256 to 364) cloned into the vector pET28a(+) was provided by Biomatik. The insert is in frame with the N-terminal His6-tag ...
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No products found
because this supplier's products are not listed.
Yufei Xiang, et al.,
bioRxiv - Bioengineering 2020
Quote:
The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Elizabeth R. Vanderwall, et al.,
bioRxiv - Immunology 2021
Quote:
... To measure SARS-CoV-2 replication in AEC cultures we used the Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit (Primerdesign®), with duplicate assays of harvested RNA from each SARS-CoV-2-infected AEC experimental condition ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Beibei Wu, et al.,
bioRxiv - Microbiology 2021
Quote:
ELISA was performed on cell culture supernatants from indicated control or SARS-CoV-2-infected cells for detection of IFN-β (PBL Assay Science, #41410) or IL-6 (ThermoFisher Scientific ...
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No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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No products found
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
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No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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No products found
because this supplier's products are not listed.
Ashok Pabbathi, et al.,
bioRxiv - Biophysics 2021
Quote:
... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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No products found
because this supplier's products are not listed.
Kushi Anand, et al.,
bioRxiv - Microbiology 2021
Quote:
... coli cultures were grown in LB medium (HIMEDIA, India). Whenever required, antibiotics were added to the culture medium (for E. coli, 100 μg/ml kanamycin (Amresco, USA) and 150 μg/ml hygromycin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Sajeet Haridas, et al.,
bioRxiv - Genomics 2023
Quote:
... heterostrophus strains sequenced by PacBio (Table S1) included highly backcrossed lab strains C5 (ATCC 48332 ...
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No products found
because this supplier's products are not listed.
Shunsuke Kataoka, et al.,
bioRxiv - Immunology 2021
Quote:
... and then pulsed with 2 μg/ml staphylococcal enterotoxin E (SEE) (Toxin Technology #ET404) for 1hr at 37°C ...
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No products found
because this supplier's products are not listed.
Roberto Espinoza-Corral, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 150 mM NaCl and 0.01% tween-20) overnight at 4°C (anti-PsbA; AS05 084A; anti-PsaB; AS10 695; anti-APC; AS08 277; Agrisera, anti-His tag; TA150087; OriGene). The membrane was washed 3 times in TBST-T at room temperature for 15 minutes each wash followed by incubation with secondary polyclonal anti-rabbit antisera HRP for one hour at room temperature in TBS-T (Jackson ImmunoResearch ...
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No products found
because this supplier's products are not listed.
J. Cody Herron, et al.,
bioRxiv - Systems Biology 2022
Quote:
... supplemented with 2% HI-FBS and 20 μM Rho kinase inhibitor Y-27632 (Hello Bio, HB2297) for 25 min of inhibition during frustrated phagocytosis ...
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No products found
because this supplier's products are not listed.
Tingting Li, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Biotinylated RBD was incubated with streptavidin (Cat 16955, AAT Bioquest) that was chemically labeled by fluorescein ...
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No products found
because this supplier's products are not listed.
Josephine Herbst, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The gRNA oligonucleotides (Table S1) were synthesized by Eurogentec. Both gRNAs were introduced into the entry vector pEn-RZ_Lb-Chimera ...
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No products found
because this supplier's products are not listed.
Kirsi Savijoki, et al.,
bioRxiv - Microbiology 2021
Quote:
... Rifampicin solution (TOKU-E) in water at a final concentration of 400 µg mL-1 corresponding to 64 x MIC (minimum inhibitory concentration ...
-
No products found
because this supplier's products are not listed.
Andrés López-Perrote, et al.,
bioRxiv - Biophysics 2020
Quote:
... coli cells (NZYTech) grown in LB medium ...
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No products found
because this supplier's products are not listed.
Khaled A. Selim, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... coli BTH101 (Euromedex) was used for BACTH assays ...
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No products found
because this supplier's products are not listed.
Nanami Sakata, et al.,
bioRxiv - Plant Biology 2022
Quote:
... L-His (Tokyo Chemical Industry), and L-Lys (Nacalai tesque ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The in vitro kinase assay (HTRF KinEASE-STK S1, CisBio 62ST1PEB) was optimized to the linear reaction range of the enzymes Lats1 (Carna 01 −123 ...
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No products found
because this supplier's products are not listed.
Nathanael A. Cruzado, et al.,
bioRxiv - Neuroscience 2020
Quote:
... The spikes were recorded using epoxy-coated tungsten electrodes (FHC) amplified by a high-input-impedance headstage filtered to extract spiking activity ...
-
No products found
because this supplier's products are not listed.
Sergiy Chornyy, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Elizabethan collars (E-collars, Kent Scientific) were used to protect the rat craniotomies during the first two weeks after the surgery.
-
Anti-Spike RBD Antibody (CR3022) is an antibody specifically targeting and binding to...
Cat# A3146, SKU# A3146-1mg*5,
1mg*5, $1690.00
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Huxley K. Hoffman, Rytis Prekeris,
bioRxiv - Cell Biology 2023
Quote:
... S1 used 100 nM bafilomycin A1 (#S1413, Selleck Chemicals, Houston, TX, USA) in the media for the final 16 hrs ...
-
No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
-
No products found
because this supplier's products are not listed.
Ashot Sargsyan, et al.,
bioRxiv - Physiology 2021
Quote:
... Crosslinking was stopped by Quenching Buffer E (Covaris). After washing ...
-
No products found
because this supplier's products are not listed.
Ana Zúñiga, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... into 500μL of Azure Hi-Def medium (Teknova, 3H5000) supplemented with 0.4% of glycerol ...
-
No products found
because this supplier's products are not listed.
Veronica Martini, et al.,
bioRxiv - Immunology 2020
Quote:
... Inhale/Exhale ratio (I/E) 1/3) or a 20 kg pig (150 ml Vt, 25 BMP, I/E ratio=1/3). Drug deposition on a filter ...
-
No products found
because this supplier's products are not listed.
Antonios Apostolopoulos, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... (e-Myco VALiD Mycoplasma PCR Detection Kit, iNtRON Biotechnology).
-
No products found
because this supplier's products are not listed.
Jason Derks, Nikolai Slavov,
bioRxiv - Systems Biology 2023
Quote:
... and mTRAQ-Δ8 mass tags (SciEx, 4440015, 4427698 and 4427700), respectively ...
-
No products found
because this supplier's products are not listed.
Kenneth W. Latimer, et al.,
bioRxiv - Neuroscience 2019
Quote:
... For S1 recordings pipettes (1.5 mm outer diameter, 0.86 inner diameter, BF150-86-10, Sutter instruments) were pulled on a PC-10 vertical puller (Narashige ...
-
No products found
because this supplier's products are not listed.
Andrew J. Stout, et al.,
bioRxiv - Bioengineering 2022
Quote:
... or Beefy-R (Hi-Def B8 supplemented with 0.4 mg/mL RPI). Beefy-9 and Beefy-R were prepared immediately before use ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Immunology 2023
Quote:
... Heat Inactivated fetal bovine serum (HI-FBS) was purchased from Atlanta Biologicals. IFN-α cytokine enzyme-linked immunosorbent assay (ELISA ...
-
No products found
because this supplier's products are not listed.
Lara K. Krüger, et al.,
bioRxiv - Cell Biology 2021
Quote:
... an TIRF-E motorized TIRF illuminator and an EMCCD Evolve camera (Photometrics). For excitation 491 nm 42 mW and 642 nm 480 mW (Gataca Systems ...
-
No products found
because this supplier's products are not listed.
Annette B. Vogel, et al.,
bioRxiv - Immunology 2020
Quote:
Purified RBD-foldon in 4 μL was applied to a glow-discharged copper grid overlaid with formvar and amorphous carbon (Ted Pella). Negative staining was performed with Nano-W organotungstate stain (Nanoprobes ...
-
No products found
because this supplier's products are not listed.
S. ter Horst, et al.,
bioRxiv - Microbiology 2022
Quote:
2’-Fluoro-2’-deoxycytidine (2’-FdC) (Biosynth-Carbosynth) was dissolved in 100% DMSO (VWR Chemicals) ...
-
No products found
because this supplier's products are not listed.
Shirsendu Ghosh, et al.,
bioRxiv - Biophysics 2022
Quote:
His-PGK-Ru was attached to the glass surface of a glass-bottom Petri dish (MatTek Corporation ...
-
No products found
because this supplier's products are not listed.
Bader Al Alwan, et al.,
bioRxiv - Biophysics 2020
Quote:
The rh E-selectin-deposited microfluidic chambers were connected to a syringe pump (Harvard Apparatus, PHD Ultra) using a silicone tubing (inner diameter of 0.8 mm ...
-
No products found
because this supplier's products are not listed.
Meagan N. Esbin, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The media was then replaced with 100µLper well supplemented F12-K medium with 500nM JF646 Halo-tag ligand and 1:1000 MembraneSteady-488nm (Biotium).
-
No products found
because this supplier's products are not listed.
Osvaldo Artimagnella, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 2% FBS (Euroclone), 1X Pen/Strept (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Vilasagaram Srinivas, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... containing 2 mm zirconia beads (#11079124Zzx, Biospec). Tissues were homogenized for 1-2 min (Mini Bead beater ...