-
No products found
because this supplier's products are not listed.
Raphael J. Eberle, et al.,
bioRxiv - Biochemistry 2021
Quote:
SARS-CoV-2 3CLpro activity assay was performed using a fluorogenic substrate DABCYL- KTSAVLQ↓SGFRKME-EDANS (Bachem, Switzerland) in a buffer containing 20 mM Tris pH 7.2 ...
-
No products found
because this supplier's products are not listed.
Ross N. England, et al.,
bioRxiv - Immunology 2023
Quote:
... diproline-modified spike protein from the SARS-CoV-2 Wuhan-Hu-1 strain were produced as previously described15,16 with m1Ψ-5-triphosphate (TriLink) instead of UTP and capped cotranscriptionally using the trinucleotide cap1 analog ...
-
No products found
because this supplier's products are not listed.
Chunhua Shi, et al.,
bioRxiv - Pathology 2020
Quote:
... Lipopolysaccharide (LPS; from E. coli O111:B4, Cedarlane;) the principal component of Gram-negative bacteria that activates the innate immune system via TLR4 ...
-
No products found
because this supplier's products are not listed.
Satoshi Imanishi, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
-
No products found
because this supplier's products are not listed.
Mu-Sen Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The His6-MBP-Cas9 fusion protein was cleaved to remove the His6-MBP tag by adding the TEV protease to the dialysis tubing (Spectrum labs) during dialysis against buffer containing 20 mM HEPES pH 7.5 ...
-
No products found
because this supplier's products are not listed.
Stefan Dannenmaier, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Polyclonal antibodies were raised against the fusion protein (Eurogentec). Immuno-reactive complexes were detected using horseradish-peroxidase coupled anti-rabbit or anti-mouse antibodies (Sigma-Aldrich/Merck ...
-
No products found
because this supplier's products are not listed.
Patricia Álvarez-Campos, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... (E,E)-methylfarnesoate (MF; Echelon Biosciences) 100 nM stock was prepared by adding 1 μl of pure hormone in 33.6 ml of DMSO in a glass container to prevent potential hydrophobic binding of the hormone to plastic ...
-
No products found
because this supplier's products are not listed.
Bob J. Ignacio, et al.,
bioRxiv - Cell Biology 2022
Quote:
... bacteria were collected by centrifugation (5,000 g, 3 min). E. coli B834 (a gift from S. van Kasteren, Leiden University) were incubated in SelenoMet medium (Molecular Dimensions) at 37 °C for 30 min to deplete intracellular methionine stores ...
-
No products found
because this supplier's products are not listed.
Simon P. Fraessle, et al.,
bioRxiv - Immunology 2022
Quote:
... 2×104 Target cells were seeded into 96 well E-Plate (ACEA Biosciences Inc.) and rested overnight ...
-
Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF)...
Cat# L-08006-001,
1 roll, USD $305.00/ea
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Azita Gorji, et al.,
bioRxiv - Cell Biology 2019
Quote:
Separated proteins were transferred to PVDF membrane in 1x FLASHBlot Transfer Buffer (Advansta) for 80 minutes at 90V at 4°C and blocked with 3% BSA for 1h at room temperature ...
-
No products found
because this supplier's products are not listed.
Hemakumar M. Reddy, et al.,
bioRxiv - Genomics 2021
Quote:
... Trypsin digested spots were processed to obtain the protein tags by MS analysis on Hybrid Quadrupole TOF mass spectrometer (API QSTAR PULSAR i, PE SCIEX).
-
No products found
because this supplier's products are not listed.
Cecilia Blikstad, et al.,
bioRxiv - Biochemistry 2023
Quote:
Final carboxysome samples and as well as the lysate were analyzed for presence of CsoSCA or sfGFP-fusion protein by SDS-page (4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad)) ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and SARS-CoV-2 Spike protein S1 (Icosagen OÜ, Estonia, cat# P-305-100) were used in this study.
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
-
Magnetofection
diificult to transfect cells
Cat# KC30396,
SilenceMag 200µL + PolyMag 100µL+ PolyMag Neo 100µL + Magnetic Plate MF96000, USD $546.00/KIT
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Shima Shahbaz, et al.,
bioRxiv - Immunology 2020
Quote:
SARS-CoV-2 stocks were pre-incubated with ViroMag transduction reagent (OZ Biosciences) as reported elsewhere31–37,38 ...
-
No products found
because this supplier's products are not listed.
Ghada Elfayres, et al.,
bioRxiv - Microbiology 2023
Quote:
HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
-
No products found
because this supplier's products are not listed.
Daniela Bottero, et al.,
bioRxiv - Immunology 2023
Quote:
... LightMix® Modular SARS-CoV (COVID19) RdRp (TIB MOLBIOL), and RNAsa P following the protocol developed by Dr ...
-
No products found
because this supplier's products are not listed.
Carmine Varricchio, et al.,
bioRxiv - Microbiology 2022
Quote:
RNA was extracted from isolated clinical SARS-CoV-2 with E.Z.N.A total RNA (Omega Bio-Tek). Maxima H Minus cDNA Synthesis (Thermofisher ...
-
No products found
because this supplier's products are not listed.
Mesfin Meshesha, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and SARS-CoV-2 lineage B.1.1.529 (Omicron Variant) culture fluid (UV inactivated, 0810642UV, Zeptometrix LLC, USA) and (heat inactivated ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
J. Rafael Ciges-Tomas, et al.,
bioRxiv - Biophysics 2022
Quote:
The synthetic gene for the SARS-CoV-2 NCTD (residues 256 to 364) cloned into the vector pET28a(+) was provided by Biomatik. The insert is in frame with the N-terminal His6-tag ...
-
No products found
because this supplier's products are not listed.
Jun Liu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... USA. β-Galactosidase (E. coli) was purchased from Abnova (catalog #P5269). Goat anti-rabbit IgG H&L-AF488 (Catalog #ab150077 ...
-
No products found
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
-
No products found
because this supplier's products are not listed.
Oh Sung Kwon, et al.,
bioRxiv - Cell Biology 2020
Quote:
... To repress protein synthesis 100 μg/ml of cycloheximide (TOKU-E) and 300 μM of puromycin (InVivoGen ...
-
No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
-
No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... Complexes of NCR-Fc fusion proteins (1 to 2 μg per staining) and PE-labeled goat anti-hIgG Fc (Dianova; 1:100 in FACS buffer) were allowed to form for 30 min before being added to cells for 60 min on ice ...
-
No products found
because this supplier's products are not listed.
Kushi Anand, et al.,
bioRxiv - Microbiology 2021
Quote:
... coli cultures were grown in LB medium (HIMEDIA, India). Whenever required, antibiotics were added to the culture medium (for E. coli, 100 μg/ml kanamycin (Amresco, USA) and 150 μg/ml hygromycin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Iliana Georgana, et al.,
bioRxiv - Microbiology 2023
Quote:
... Proteins were transferred to 0.45 μm nitrocellulose membranes (GVS) using a Trans-Blot semi-dry transfer unit (Bio-Rad) ...
-
No products found
because this supplier's products are not listed.
Shunsuke Kataoka, et al.,
bioRxiv - Immunology 2021
Quote:
... and then pulsed with 2 μg/ml staphylococcal enterotoxin E (SEE) (Toxin Technology #ET404) for 1hr at 37°C ...
-
No products found
because this supplier's products are not listed.
Claire A. Ruddiman, et al.,
bioRxiv - Physiology 2022
Quote:
... Protein was transferred to a nitrocellulose membrane (Genesee, 84-876) at 100V for 1 hour ...
-
No products found
because this supplier's products are not listed.
Andrés López-Perrote, et al.,
bioRxiv - Biophysics 2020
Quote:
... coli cells (NZYTech) grown in LB medium ...
-
No products found
because this supplier's products are not listed.
Khaled A. Selim, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... coli BTH101 (Euromedex) was used for BACTH assays ...
-
No products found
because this supplier's products are not listed.
Nanami Sakata, et al.,
bioRxiv - Plant Biology 2022
Quote:
... L-His (Tokyo Chemical Industry), and L-Lys (Nacalai tesque ...
-
No products found
because this supplier's products are not listed.
Jie Ding, et al.,
bioRxiv - Physiology 2022
Quote:
... and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen). All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences ...
-
No products found
because this supplier's products are not listed.
Sara Y. Brucker, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and the desired amount of cell suspension mixed with Basement Membrane Extract (Type 2, 3533-001-2, Trevigen; BME) at a ratio of 65% BME to 35% cell suspension ...
-
No products found
because this supplier's products are not listed.
Daichi Kamiyama, et al.,
bioRxiv - Neuroscience 2022
Quote:
... controlled with Fusion software (Oxford Instruments).
-
No products found
because this supplier's products are not listed.
Mohammed Abdullahel Amin, et al.,
bioRxiv - Cell Biology 2022
Quote:
... anti-CENP-E (Boster, M04553-1) for WB at 1:1000 ...
-
No products found
because this supplier's products are not listed.
Erika Cecon, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Lumi4-Tb-labelled ACE2 cells were incubated with d2-labelled anti-FLAG tag antibody (2 μg/mL, 1h at room temperature; 61FG2DLF, Cisbio Bioassays), followed by addition of non-labelled RBD (5 nM ...
-
No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
-
Mitochondrial fusion promoter M1 is a mitochondrial dynamic modulator that increases the...
Cat# S3375, SKU# S3375-25mg,
25mg, $290.00
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Juliane Tschuck, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Guggulsterone E&Z (FXR antagonist, Selleck Chemicals), iFSP1 (FSP1 inhibitor ...
-
No products found
because this supplier's products are not listed.
Joy Twentyman, et al.,
bioRxiv - Immunology 2023
Quote:
... Membranes were blocked with 2% w/v milk (Research Products International, Mt. Prospect, IL, #M17200-500.0) and 2% w/v bovine serum albumin (Sigma-Aldrich #A7906-100G ...
-
No products found
because this supplier's products are not listed.
Ana Zúñiga, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... into 500μL of Azure Hi-Def medium (Teknova, 3H5000) supplemented with 0.4% of glycerol ...
-
No products found
because this supplier's products are not listed.
Ranjan Sengupta, et al.,
bioRxiv - Cell Biology 2019
Quote:
Cell pellets (2-3 μl) were loaded onto copper membrane carriers (1mm x 0.5 mm; Ted Pella Inc.) and cryofixed using the EM PACT2 high pressure freezer (Leica) ...
-
No products found
because this supplier's products are not listed.
Anil Kumar Ganga, et al.,
bioRxiv - Cell Biology 2020
Quote:
Coverslips with fixed NIH-3T3 and RPE cells were imaged using a Nikon Eclipse Ti-2 widefield microscope equipped with a CMOS camera (Photometrics Prime BSI or Hamamatsu Orca-Fusion), a 60✕ PlanApo oil objective (NA 1.40 ...
-
No products found
because this supplier's products are not listed.
Or Sharon, et al.,
bioRxiv - Plant Biology 2022
Quote:
... PCR reactions for plant samples were supplemented with a custom peptide nucleic acid (PNA) blocker (ITS1PNABlk: 50-O-E–E-GTCGTGTGGATTAAA-30; PNA BIO INC, Thousand Oaks, CA) to block host plant ITS sequences ...
-
No products found
because this supplier's products are not listed.
Brett Horr, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Most of our fusions were subsequently performed using electrofusion (BTX, Harvard apparatus) using 10 million splenocytes and 10 to 30 million SP20 per fusion to obtain a similar number of hybridomas (approximately 1000) ...
-
No products found
because this supplier's products are not listed.
Finola E. Cliffe, et al.,
bioRxiv - Bioengineering 2022
Quote:
... A membrane (adhesive layer of breath easy polyurethane (PU) membrane from Diversified Biotech, Dedham ...
-
No products found
because this supplier's products are not listed.
Alexandra C. Nowlan, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Recording depth was measured by a piezoelectric micromanipulator (Sutter Instrument SOLO/E-116). All neurons were found from 300 μm – 1200 μm from the cortical surface ...
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
-
No products found
because this supplier's products are not listed.
Aditi Sharma, et al.,
bioRxiv - Immunology 2020
Quote:
... following exposure to e-cig vapor infused media using the STX2 electrodes with digital readout by EVOM2 (WPI). For automated assessments ...