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SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli
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Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Cell doi: 10.1016/j.cell.2020.09.033Quote: SARS-CoV-2 (2019-nCoV) spike Protein (RBD, His Tag) (Sino Biologicals, 40592-V08B), SARS-CoV-2 (2019-nCoV ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Cell doi: 10.1016/j.cell.2020.09.033Quote: ... and SARS-CoV-2 spike protein (ECD, His & Flag Tag) (GenScript Z03481). Proteins were biotinylated using EZ-Link™ Sulfo-NHS-Biotin ... -
Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 genome was amplified by using Ion AmpliSeq SARS-CoV-2 Research Panel (ThermoFisher Scientific, USA) that consists of two pools with amplicons ranging from 125 bp to 275 bp in length and covering >99% of the SARS-CoV-2 genome ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2021Quote: RNA isolation from SARS-CoV and SARS-CoV-2 infected A549-ACE2 cells was performed as described above (Qiagen). 500 ng total RNA was used for reverse transcription with PrimeScript RT with gDNA eraser (Takara) ... -
GE Life Sciences
No products found because this supplier's products are not listed.Cited in An Engineered Antibody with Broad Protective Efficacy in Murine Models of SARS and COVID-19bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences). -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020, published in Nature Microbiology doi: 10.1038/s41564-020-00846-zQuote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2021Quote: ... The SARS-CoV-2 viral load was quantified in culture supernatant using RT-QPCR with primer probes targeting E gene of SARS-CoV-2 using PrimeDirect Probe RT-qPCR Mix (TaKaRa Bio USA, Inc) and Applied Biosystems QuantStudio3 real-time PCR system (Applied Biosystems ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2021Quote: Spike display plasmids incorporate the pre-fusion stabilized SARS-CoV-2 S-6P (“HexaPro”) as the reference sequence for all spike variants (Addgene #154754)37 ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2018.02.016Quote: ... The encoded N-terminal His-tagged fusion protein was overexpressed in Escherichia coli BL21 (DE3) Codon plus RIL (Stratagene) cells at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen) ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (ab273068; Lots GR3356031-1 and 3353172-2; Accession MN908947) was purchased from Abcam. The protein was reconstituted in sterile water for functional studies ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020, published in Cell doi: 10.1016/j.cell.2021.01.035Quote: ... patients with at least one nasopharyngeal swab positive for SARS-CoV-2 as determined by qRT-PCR (Roche LightCycler480 ... -
Genetex
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020, published in Journal of Biological Chemistry doi: 10.1016/j.jbc.2021.100306Quote: SARS-Cov-2 spike protein antibody is from GeneTex (GTX632604). ACE2 antibody is from GeneTex (GTX01160) ... -
Acrobiosystems
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020, published in Analytical Chemistry doi: 10.1021/acs.analchem.0c03173Quote: Recombinant SARS-CoV-2 spike (R683A, R685A, His-tag) protein expressed in HEK 293 cell line was obtained from Acrobiosystems (Newark, DE, USA). Trypsin Gold and Glu-C ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2020Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020Quote: ... 1 x 108 induced cells were stained with 1 µM of directly AF488-labeled SARS-CoV-2 RBD and 0.5 µM anti-HA AF647 (Cell Signaling Technology) antibody ... -
Abbott
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... to confirm absence of SARS-CoV-2 infection and by serology to confirm presence of anti-SARS-COV-2 IgG (Architect SARS-CoV-2 IgG assay, Abbott Ireland ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in Journal of Pharmacology and Experimental Therapeutics doi: 10.1124/jpet.119.261180Quote: ... The Western blot membranes were stained with HRP anti-His tag antibody (Biolegend, #652504) diluted 1:2,000 in 0.5% (w/v ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2020Quote: ... transferred onto nitro cellulose membranes and blotted with 1:1000 Anti-His-HRP (6xHis Epitope TAG, Cat. no. sc-8036 HRP, Santa Cruz Inc) and 1:500 anti-actin-HRP (cat ... -
Mabtech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: The effect of neutralization capacity of Multi-DARPin was evaluated by exposing serial dilutions of the DARPin candidates to increasing titers of SARS-CoV-2 and determining cell protection by CellTiter-Glo assay (Promega, Madison, USA). Serial dilution of DARPin candidates were prepared in 96 well plates in 100 μl cell culture medium (2%-FBS-MEM + HSA ... -
Bioss
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... An anti-mouse SARS-CoV-2 nucleocapsid protein (Clone 1C7, Bioss Antibodies) primary antibody solution was prepared at 1 mg/mL in PBS + 1% non-fat milk and added to all wells for one hour at room temperature ... -
Invivogen
No products found because this supplier's products are not listed.bioRxiv - Immunology 2017Quote: ... 1 μg/ml E-coli DNA (dsDNA E-coli Invivogen), or 0.5 μg/ml cff-DNA ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020, published in Nature doi: 10.1038/s41586-020-2280-2Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... PBMCs were unstimulated (DMSO) or stimulated with SARS-CoV-2 spike (S) peptide pools (5 mg/mL) in presence of GolgiPlug (BD Biosciences, San Jose, CA) for 6 hours ... -
BPS Bioscience
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2021Quote: The ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (BPS Bioscience #79936) was purchased and measured the binding of ACE2 and SARS-CoV-2 Spike in the presence and absence of inhibitors ... -
Lucigen
No products found because this supplier's products are not listed.bioRxiv - Genomics 2016, published in Nature Methods doi: 10.1038/nmeth.4177Quote: ... coli cells (Lucigen #60242-2) using pre-chilled 1 mm electroporation cuvettes (BioRad #1652089 ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: The ACCESS SARS-CoV-2 CLIA (Beckman Coulter Inc., California, USA) was used for semiquantitative detection of IgG directed against S protein RBD using serum obtained from venipuncture blood ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... Anti-His-Tag (Proteintech, 66005-1-Ig), Anti-βIII tubulin (Thermo Scientific Pierce ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Pathology 2021Quote: Spike RBD proteins were labeled using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies) following manufacturer protocol at 2:1 protein to dye ratio ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ... -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2017, published in Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1802233115Quote: ... or (2) epifluorescence microscopy (Nikon Ti-E) (Fluorescence microscopy) ... -
RayBiotech
No products found because this supplier's products are not listed.bioRxiv - Pathology 2020, published in Vascular Pharmacology doi: 10.1016/j.vph.2020.106823Quote: ... or the recombinant SARS-CoV-2 spike protein RBD (RayBiotech) that only contains the RBD region (Arg319 - Phe541 ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: Anti-SARS-CoV-2 IgG titers were determined using Human Anti-SARS-CoV-2 Virus Spike 1 (S1) IgG assay (Alpha Diagnostic). hIVIG batches were tested using multiple serial dilutions and a curve constructed by plotting the log of the optical density as a function of the log of the dilution ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2021Quote: ... and a SARS-CoV-2 S peptide pool (Miltenyi Biotec, Germany) for immune reaction together with 10 μg/ml brefeldin A ... -
Molecular Devices
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020, published in Nature doi: 10.1038/s41586-020-2349-yQuote: ... His-tagged RBD of SARS-CoV or SARS-CoV-2 were loaded at 3 µg/ml in KB for 15 minutes onto anti-HIS (HIS2) biosensors (Molecular Devices, ForteBio). Association of mAb and Fab was performed in KB at 15 μg/ml and 5 μg/ml respectively for 5 minutes ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and 1% P/S were incubated with 25 µl of SARS-CoV-2 PSV at 37°C for 1 h in 96-well half-well plates (Corning, 3688). After the incubation ... -
Sartorius
No products found because this supplier's products are not listed.Cited in An Engineered Antibody with Broad Protective Efficacy in Murine Models of SARS and COVID-19bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ... -
LI-COR
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0195356Quote: ... for 10-His tag on Med17 and scanning with an Odyssey infrared imaging system (LI-COR Biosciences). Bands were quantified using ImageJ software package [47] ... -
Pall
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018Quote: ... The proteins were electro-transferred to PVDF membrane (Pall). Primary antibodies were incubated at 4°C overnight and the HRP-conjugated secondary antibodies were incubated at room temperature for 1 hr ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018, published in G3: Genes|Genomes|Genetics doi: 10.1534/g3.118.200814Quote: ... The libraries of one affected puppy and his dam (dog A and E) were sequenced on an Illumina NextSeq500 (Illumina, San Diego, CA, USA) using the 2×150bp in paired-end mode ... -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Life Science Alliance doi: 10.26508/lsa.201800108Quote: Co-IPs of GFP-fusion or Strep-tag fusion proteins were done with GFP-Trap_A (Chromotek) or Strep-Tactin affinity chromatography using Strep-Tactin sepharose beads (IBA) ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ... -
Avanti Polar Lipids
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in eLife doi: 10.7554/eLife.53479Quote: ... E coli polar lipids (Avanti Polar Lipids, Alabaster, AL) in chloroform were dried under argon in a round-bottomed flask ... -
MP Biomedicals
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019Quote: Filter membranes were aseptically removed from the Sterivex cartridge and transferred to 2 ml Lysing Matrix E tubes (SKU 116914100, MP Biomedicals, USA) and DNA extraction and purification were performed in a Maxwell® 16 DNA extraction system (Promega ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018, published in genesis doi: 10.1002/dvg.23259Quote: An Amotl1 expression construction encoding an AMOTL1-myc fusion protein tag was purchased from Origene (#RC207693, Rockville, MD). Site directed mutagenesis to recreate the R157C variants was performed with QuikChange Lightning Site-Directed Mutagenesis kit (Agilent ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2020, published in Langmuir doi: 10.1021/acs.langmuir.0c00130Quote: ... coli constitutively expressing the Green Fluorescent protein present on biofilms was examined by laser scanning confocal microscopy (Leica TCS SP8) using oil immersion at 40X magnification ...