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No products found
because this supplier's products are not listed.
Mu-Sen Liu, et al.,
bioRxiv - Biochemistry 2019
Quote:
... The His6-MBP-Cas9 fusion protein was cleaved to remove the His6-MBP tag by adding the TEV protease to the dialysis tubing (Spectrum labs) during dialysis against buffer containing 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Yuan Zhang, Zhigang Liu, Bin Zhang,
bioRxiv - Cell Biology 2022
Quote:
... FV antigen was quantified by ELISA using matched-pair antibody set for human FV antigen from Affinity Biologicals. All assays were performed according to manufacturers’ instructions.
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No products found
because this supplier's products are not listed.
Shaun M. Christie, et al.,
bioRxiv - Biophysics 2021
Quote:
... Recombinant human Semaphorin 3A (CX65, Bon Opus Biosciences, Milburn, NJ) contains residues 21-771 and is >95% pure ...
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No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
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No products found
because this supplier's products are not listed.
Jianwen Zhou, et al.,
bioRxiv - Cell Biology 2022
Quote:
GST and GST-fusion proteins were expressed in Escherichia coli strain SoluBL21 (DE3) (Genlantis, #C700200). Protein expression was induced by adding 50 g/ml isopropyl β-D-1- thiogalactopyranoside (IPTG) ...
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No products found
because this supplier's products are not listed.
Kinya Nomura, et al.,
bioRxiv - Plant Biology 2023
Quote:
... AD and BD fusion proteins were detected with a chicken HA epitope antibody (Aves Labs) and a rabbit LexA binding domain antibody (Clontech) ...
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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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No products found
because this supplier's products are not listed.
Yanzhao Zhang, et al.,
bioRxiv - Microbiology 2020
Quote:
... The p24 antigen levels in viral supernatants were measured by an HIV-1 p24 antigen capture ELISA (XpressBio). Transfection efficiencies were normalized to the luciferase activity ...
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No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Inc., Cat# CKH113).
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No products found
because this supplier's products are not listed.
Georgina S.F. Anderson, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... THE™ NWSHPQFEK Tag-biotin (Cambridge Bioscience), anti-CD69-APC (clone FN50 ...
-
No products found
because this supplier's products are not listed.
Megha Rajendran, et al.,
bioRxiv - Biophysics 2022
Quote:
... FCS measurements were made in eight-well cover-glass slides (Grace Biolabs) pretreated with Sigmacoat (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Wiktoria Ogrodzińska, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
-
No products found
because this supplier's products are not listed.
Marcin Poreba, et al.,
bioRxiv - Biochemistry 2019
Quote:
... Fluorescent tags (Cyanine-5 NHS and Cyanine-7 NHS) were purchased from Lumiprobe (Hannover, Germany). Diazomethane was generated according to the Aldrich Technical Bulletin (AL-180 ...
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No products found
because this supplier's products are not listed.
Gila Lustig, et al.,
bioRxiv - Microbiology 2019
Quote:
... Supernatant containing released virus was harvested two days post-transfection and filtered through a 0.45 micron filter(GVS) and stored in 0.5ml aliqouts at −80°C ...
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No products found
because this supplier's products are not listed.
Herman KH Fung, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Confocal volume estimation was carried out by ten 1-min FCS measurements of 10 nM Atto488 (ATTO-TEC) in water ...
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No products found
because this supplier's products are not listed.
Nadine R King, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 2 mg/ml Human Serum Albumin (HSA; Irvine Scientific), 10 μg/ml insulin (Sigma) ...
-
No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Crystallisation of recombinant Cys232Ser α1-antitrypsin was carried out in 2-well MRC crystallisation plates with a Mosquito robot (TTP Labtech) using 100nl protein solution and 100nl well solution ...
-
No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
-
No products found
because this supplier's products are not listed.
Sofia Rossini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... while recombinant human IDO1 protein was obtained by Giotto Biotech. Construct expressing murine Src was obtained from Origene.
-
No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... hPC antigen (hPC:Ag) was measured using the Human Protein C AssayMaxTM ELISA Kit (Assaypro, St. Charles, MO) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Emma T Crooks, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by alkaline phosphatase labeled anti-human Fc conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT (Sigma).
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No products found
because this supplier's products are not listed.
Joanna M. Cooper, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and IV Fc chimera proteins were produced by Molecular Innovations. His-tagged recombinant human tau variants 2N4R ...
-
No products found
because this supplier's products are not listed.
Mahima Sharma, et al.,
bioRxiv - Neuroscience 2023
Quote:
... periodic monitoring was conducted of respiratory and heart rates (Physio Suite, Kent Scientific), muscle tone ...
-
No products found
because this supplier's products are not listed.
Cian Schmitt-Ulms, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Spliceosome proteins for fusion experiments were ordered as eBlocks from IDT DNA. An expression backbone originally containing wild-type Cas7-11 (pDF0506 ...
-
No products found
because this supplier's products are not listed.
Pehuén Pereyra Gerber, et al.,
bioRxiv - Microbiology 2021
Quote:
... incubated for 30 min with a PE-labeled anti–human IgG-Fc antibody (Leinco/Biotrend), washed again ...
-
No products found
because this supplier's products are not listed.
Nandan Haloi, et al.,
bioRxiv - Biophysics 2020
Quote:
... followed by addition of HKII (human recombinant HKII, 60 kU/ml; Genway Biotech, Slan Diego, CA) into the cis chamber ...
-
No products found
because this supplier's products are not listed.
Katrin Manske, et al.,
bioRxiv - Bioengineering 2023
Quote:
20,000 Human Embryonic Kidney cells (HEK293-CD19+) expressing the CD19 antigen were seeded on a 96-well E-plate (ACEA Biosciences) over night ...
-
No products found
because this supplier's products are not listed.
Shahrnaz Kemal, et al.,
bioRxiv - Neuroscience 2023
Quote:
... All vectors contain an N-terminal 6x-His-tag, C terminal Strep-Tag II tag, with or without a N or C terminal fluorophore (EGFP, mNeonGreen (Allele Biotech), or mRuby2).
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No products found
because this supplier's products are not listed.
Mirjam C van der Net, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... was recloned in a pDONR221 backbone and the Myc-tag was replaced with a Flag-tag (Epoch Life Science). The sequence 5’-CAATGCGGAATATCAATCCCAGCACAGCAAATTCTCCA AAATGTCAGG-3’ was added between base pairs 982 and 983 of the YAP1 gene ...
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Human IgG1 isotype control is suitable for use as a non-targeting isotype control in various in...
Cat# A2051, SKU# A2051-1mg*5,
1mg*5, $279.00
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Allen L. Pan, et al.,
bioRxiv - Neuroscience 2023
Quote:
... human SCF (20 ng/mL) and 10 μM Rho-associated protein kinase inhibitor (ROCKi, Selleck Chemicals). Embryoid bodies were fed every day from day 1 to day 3 ...
-
No products found
because this supplier's products are not listed.
Lindsey R. Conroy, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Recombinant PNGaseF Prime was obtained from Bulldog Bio, Inc ...
-
No products found
because this supplier's products are not listed.
Djem U. Kissiov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... In all cases medium contained 5% FCS (Omega Scientific), 0.2 mg/mL glutamine (Sigma) ...
-
No products found
Yuanyuan He, et al.,
bioRxiv - Microbiology 2023
Quote:
Imaging plates for virus quantification were prepared by coating coverslip-bottom wells (Cellvis) with 0.18 mg/ml BSA-biotin in PBS overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Balaji Karthick Subramanian, et al.,
bioRxiv - Pathology 2019
Quote:
... Human primary podocytes from Celprogen Inc ...
-
No products found
because this supplier's products are not listed.
Nicolas Lamassiaude, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Recombinant constructs were purified using EZNA Plasmid DNA Mini kit (Omega Bio-Tek) and sequence-checked (Eurofins Genomics) ...
-
No products found
because this supplier's products are not listed.
Yanhua Du, et al.,
bioRxiv - Epidemiology 2019
Quote:
The genomes of SFTS virus isolates were compiled using the SeqMan program in the LaserGene software package (DNAStar). The percentage similarities of nucleotide identity or amino acid identity were calculated using the ClustalX software[16] ...
-
No products found
because this supplier's products are not listed.
JF Sturgill, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AAV virus (300nL volume) was then pressure injected 100nL/min via a glass pipette pulled (P-97 Sutter Instruments) from borosilicate capillaries (Drummond calibrated 5ul ...
-
No products found
because this supplier's products are not listed.
Hazel Stewart, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Pacific Blue-conjugated anti-CD71 (Exbio, MEM-75, FC 1 μg / 100 μl / 106 cells), StrepMAB-Classic (IBA LifeSciences ...
-
No products found
because this supplier's products are not listed.
Jia Wang, Xincheng Zhong, Hang Yin,
bioRxiv - Immunology 2023
Quote:
Recombinant TRAF6(50-350) and other variants were labeled with Cyanine 3 (AAT Bioquest), UBE2N ...
-
No products found
because this supplier's products are not listed.
K.M. Tharp, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... OTI CD8+ T cells were isolated and co-cultured with PyMT-mCherryOVA tumor cells 61 that display the model antigen chicken ovalbumin (SIINFEKL) in 25 mM glucose DMEM (Gen Clone, 25-500) supplemented with or without L-ornithine (RPI, 020040).
-
No products found
because this supplier's products are not listed.
M. Julhasur Rahman, et al.,
bioRxiv - Microbiology 2021
Quote:
The mCherry-E3L integration sites in the purified HGT virus genomes were located by inverse PCR amplification and by PacBio sequencing ...
-
No products found
because this supplier's products are not listed.
Alicja Przybyszewska-Podstawka, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... TGFβ (human TGFβ1, Biorbyt, Cambridge, United Kingdom), Gibson Assembly® Master Mix (NEB) ...
-
No products found
because this supplier's products are not listed.
Danya Abazari, et al.,
bioRxiv - Neuroscience 2022
Quote:
Protein palmitoylation assay was performed using CAPTUREome S-palmitoylated protein kit (Badrilla, Leeds, UK), as described by manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
-
No products found
because this supplier's products are not listed.
Ida M. Modvig, et al.,
bioRxiv - Physiology 2019
Quote:
... Radioactive labeled rat and human secretin was obtained from Phoenix Pharmaceuticals, Inc (cat no ...
-
No products found
because this supplier's products are not listed.
Qinghui Wang, et al.,
bioRxiv - Immunology 2022
Quote:
... Naïve CD3 human T cells were purchased from HemaCare (Lot #21068415). N/TERT-1 cells were a gift from the Rheinwald Lab (Dickson et al. ...
-
No products found
because this supplier's products are not listed.
Udoka C. Nwangwu, et al.,
bioRxiv - Zoology 2024
Quote:
... 15 ml Fetal Calf Serum (FCS) 3% and 5ml Amphotericin B) and 500ml of Zirconia beads (Firma Biospec: 2.0 mm, Cat. No 1107912). The contents were macerated using the Qiagen Tissuelyser LT for 10 min and then centrifuged at 4,500 x g for 15 min ...
-
No products found
because this supplier's products are not listed.
Heon Shin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
DNA methyltransferase activities of endogenous DNMT1 and recombinant DNMT1 were measured by EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (P-3139-48, EpiGentek, Farmingdale, NY, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... gels were either stained with Coomassie (Protein Ark) or transferred to PVDF membranes for Western blot analysis.