-
No products found
because this supplier's products are not listed.
Youssouf Sereme, et al.,
bioRxiv - Microbiology 2020
Quote:
... The virus-infected MRC5 cells were collected and centrifuged at 700 × g for 15 minutes (Eppendorf Centrifuge 5810R). Supernatant was filtered by serial filtration steps to eliminate cells and cellular debris ...
-
No products found
because this supplier's products are not listed.
Roman Praschberger, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 5 μg of head lysate or 5 μg of Human Normal Brain GenLysate (G-Biosciences) was subjected to standard SDS-PAGE using a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Zhifen Cui, et al.,
bioRxiv - Microbiology 2021
Quote:
... A pathology disease index of respiratory disease was created using semi-quantitative scoring on digitized lung sections (Aperio AT2, Leica Biosystems) with Imagescope (Leica Biosystems ...
-
No products found
because this supplier's products are not listed.
Gary Baisa, et al.,
bioRxiv - Immunology 2020
Quote:
... an RBD-Fc fragment (319-541) with a C-terminal human Fc-tag was purchased from BPS Bioscience (San Diego, CA). Recombinant biotinylated ACE2 was purchased from Acro Biosystems (Newark ...
-
No products found
because this supplier's products are not listed.
Jerry Yan, et al.,
bioRxiv - Bioengineering 2023
Quote:
... plated at 800,000 splenocytes/well and restimulated with 10 μg rabies virus glycoprotein peptide (AnaSpec) for 24h at 37 °C ...
-
No products found
because this supplier's products are not listed.
Lisa Dettinger, et al.,
bioRxiv - Microbiology 2021
Quote:
... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ...
-
No products found
because this supplier's products are not listed.
Haizhang Chen, et al.,
bioRxiv - Immunology 2020
Quote:
... or a PE-TexasRed-conjugated human IgG-Fc fragment (Rockland) for 1h at 4°C in PBS/3%FCS ...
-
No products found
because this supplier's products are not listed.
Jui-Hsien Chang, et al.,
bioRxiv - Neuroscience 2020
Quote:
... AFL was labeled at the Fc portion of human IgG immunoglobulin (goat polyclonal anti-human IgG Fc antibody, NB7446, Novus Biologicals, 1:200 at 4C overnight). Stainings were followed by a corresponding Alexa Fluor-conjugated secondary antibody (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Fangzhou Lou, et al.,
bioRxiv - Immunology 2020
Quote:
... Normal Human Epidermal Keratinocytes (NHEKs; Lifeline Cell Technology, cat. FC-0007) stored in liquid nitrogen were defrosted and cultured in serum-free basal medium with growth factors (Lifeline Cell Technology ...
-
No products found
because this supplier's products are not listed.
Aske Krogsgaard, et al.,
bioRxiv - Neuroscience 2022
Quote:
... we monitored end-tidal respiratory CO2 levels (Type 340 capnograph; Harvard Apparatus) and arterial blood pressure via a femoral artery catheter (BP-1 Monitor ...
-
No products found
because this supplier's products are not listed.
Pascal Brandys, et al.,
bioRxiv - Immunology 2022
Quote:
... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
-
No products found
because this supplier's products are not listed.
Simona Selberg, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
-
No products found
because this supplier's products are not listed.
CL Esposito, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Amanda M. Travis, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and C-terminal MYC tag was placed in front of human NPHP3 (GeneCopoeia GC-H2370). All mutations to replace the lipidated cysteine with alanine were made using PCR mutagenesis (Weiner et al. ...
-
No products found
because this supplier's products are not listed.
Johannes Oppermann, et al.,
bioRxiv - Biophysics 2023
Quote:
... Virus suspensions were slowly injected (100 nl min−1) using a 34 G beveled needle (Nanofil syringe, World Precision Instruments). After injection ...
-
No products found
because this supplier's products are not listed.
David Andruszewski, et al.,
bioRxiv - Neuroscience 2023
Quote:
... resuspended in PBS/FCS supplemented with Fc-block (BioXCell) and incubated for 15min on ice ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Jamin Liu, et al.,
bioRxiv - Microbiology 2022
Quote:
... 293T cells were transfected with arenavirus glycoprotein expression plasmids using TransIT-LT1 (Mirus Bio). Cells were transduced the following day with VSV-ΔG-GFP (Kerafast)(45 ...
-
No products found
because this supplier's products are not listed.
Eugen Widmeier, et al.,
bioRxiv - Genetics 2019
Quote:
... and then subjected to spectrophotometric analysis for isolated respiratory chain complex activities at 37°C using a spectrophotometer (PerkinElmer). Complex II activity was measured at 600 nm (ε = 19.1 mmol-1cm-1 ...
-
No products found
Konstantina Nikolatou, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... V5-Tag (ABM, G189). Following three PFS washes ...
-
No products found
because this supplier's products are not listed.
Harman Malhi, et al.,
bioRxiv - Immunology 2022
Quote:
... Anti-Human Fc biosensors (ForteBio, catalog #18-0015) were submerged in wells of black 96-well microplates (Greiner, catalog #655209) containing 250 μL of kinetics buffer (PBS ...
-
No products found
because this supplier's products are not listed.
Zhenhui Zhong, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Anti-HA tag antibodies (ABclonal) were coupled to magnetic beads by Dynabeads antibody coupling kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Matthew Gagne, et al.,
bioRxiv - Immunology 2022
Quote:
... SARS-CoV-2 S-pseudotyped virus was prepared by transfection in 293T/17 cells (human embryonic kidney cells in origin; obtained from American Type Culture Collection, #CRL-11268) using a lentivirus backbone vector ...
-
No products found
because this supplier's products are not listed.
Martina Hauke, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 10% FCS (PromoCell, Germany). HEK-NF-κB_luc were supplemented for routine growth with 50 µg/mL Hygromycin B (Invivogen ...
-
No products found
because this supplier's products are not listed.
Mallory Genest, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
-
No products found
because this supplier's products are not listed.
Takahiro Yamada, et al.,
bioRxiv - Microbiology 2019
Quote:
... 2 % FCS (MP Biomedicals, Santa Ana, CA), 100 U/mL penicillin ...
-
No products found
because this supplier's products are not listed.
Anwesha Sanyal, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and a 50% resin solution (24 g Embed 812, 9 g DDSA, 15 g NMA, 1.2 g BDMA; 14121; Electron Microscopy Sciences) in propylene oxide ...
-
No products found
because this supplier's products are not listed.
Yusha Wang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
The plasmid was interrupted with g-TUBE (Covaris g-TUBE520079), and the plasmid was repaired with New England Biolabs PreCR® Repair MixM0309L ...
-
No products found
because this supplier's products are not listed.
Imke M.E. Schuurmans, et al.,
bioRxiv - Neuroscience 2024
Quote:
... RevitaCell™ and 2.5% AM FCS (ScienCell®; 0010). Then medium of the neurons was refreshed with neurobasal medium supplemented with B-27 ...
-
No products found
because this supplier's products are not listed.
Philip T. Coleman, et al.,
bioRxiv - Neuroscience 2023
Quote:
Adult male (275-300 g) and female (225-250 g) Sprague–Dawley rats (Envigo) were pair-housed on a 12-h light–dark cycle (lights on at 0700 h ...
-
No products found
because this supplier's products are not listed.
Angela Ma, et al.,
bioRxiv - Microbiology 2022
Quote:
... adenovirus (D3Ultra Respiratory Virus Screening & Identification kit, Quidel, San Diego, CA), and cytomegalovirus (D3 DFA Cytomegalovirus Immediate Early Antigen Identification kit ...
-
No products found
because this supplier's products are not listed.
Jieqiong Qu, et al.,
bioRxiv - Microbiology 2023
Quote:
... CHIKV virus stock (5 × 108 TCID50/mL) and full human blood (Sanquin) was 1:2 mixed to make the infectious blood meal with a final virus titer of 1.7× 108 TCID50/ml ...
-
No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... sapiens-wt) glycoprotein from Advanced Biomart (San Gabriel, CA, USA) and the anti-Ebola surface glycoprotein [KZ52] from Absolute Antibody (Oxford, GB). The obtained EBOV-GP was expressed with a polyhistidine-tag at the C-terminus and consists of 629 amino acids ...
-
No products found
because this supplier's products are not listed.
Siti Naqiah Amrun, et al.,
bioRxiv - Immunology 2019
Quote:
... virus inoculum was removed and replaced with IMDM with 10% human serum (HS; Innovative Research Inc). Cells were incubated at 37°C and harvested at time-points 0 ...
-
No products found
because this supplier's products are not listed.
José-María Díez, Carolina Romero, Rodrigo Gajardo,
bioRxiv - Immunology 2020
Quote:
... Human Anti-SARS-CoV-2 Virus Spike 1 [S1] IgG ELISA Kit (Alpha Diagnostic Intl. Inc.), against S1 subunit spike protein ...
-
No products found
because this supplier's products are not listed.
Salim Benlefki, et al.,
bioRxiv - Neuroscience 2022
Quote:
... enhancer for ligand and recombinant human Fas-Fc were purchased from Enzo Life Sciences. z-IETD-fmk ...
-
No products found
because this supplier's products are not listed.
James R. Byrnes, et al.,
bioRxiv - Immunology 2021
Quote:
... beads were collected and glycoproteins reduced with 5 mM TCEP (Calbiochem) for 30 minutes at 37°C and alkylated with 11 mM iodoacetamide (Sigma ...
-
No products found
because this supplier's products are not listed.
Paola Munoz-Tello, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
-
No products found
because this supplier's products are not listed.
Allison M. Owen, et al.,
bioRxiv - Immunology 2022
Quote:
ROS production by BMDMs (1X105 events) was assessed using a commercially available kit (Respiratory Burst Assay, Cayman Chemical) according to the manufacturer’s protocol with the exception of stimulating the cells with PMA ...
-
No products found
because this supplier's products are not listed.
Juan Jauregui-Lozano, et al.,
bioRxiv - Genomics 2021
Quote:
CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
-
No products found
because this supplier's products are not listed.
Yu-Feng Chien, et al.,
bioRxiv - Bioengineering 2020
Quote:
... a TAG lens (TAG lens 2.5β, TAG Optics) was placed directly on top of an objective (UPLFLN 10XP, Olympus) to enable high-speed axial scan of the focus ...
-
No products found
because this supplier's products are not listed.
Ellen Van Gulck, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 48h post transfection virus was collected and concentrated with PEG Virus Precipitation Kit (BioVision Inc, USA) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
José A. González-Feliciano, et al.,
bioRxiv - Biochemistry 2020
Quote:
... The 2G12 and PG16 bNAbs in 0.5X Kinetics Buffer (PBS with 0.02% Tween and 0.05 mg/mL BSA) were loaded onto an anti-Human IgG Fc Capture sensor (Pall ForteBio Corp). Binding kinetics were determined using serial dilutions of CHO-K1-produced and Expi293F-produced gp145 ...
-
No products found
because this supplier's products are not listed.
Julianne B. Riggs, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were subjected to Fc receptor blocking using Fc Shield (Tonbo Biosciences) for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Matthieu P.M.H. Benoit, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the SNAPf-EGFP-6His tag was replaced with a strep-II tag (IBA Lifesciences GmbH) using Q5 mutagenesis (New England Biolabs Inc. ...
-
No products found
because this supplier's products are not listed.
James Brett Case, et al.,
bioRxiv - Microbiology 2020
Quote:
... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
-
No products found
because this supplier's products are not listed.
Till Stephan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Spot tag (ChromoTek, Planegg-Martinsried, Germany), TOMM22 (Merck ...
-
No products found
because this supplier's products are not listed.
Nicolas Rose, et al.,
bioRxiv - Bioengineering 2021
Quote:
... PLL-g-PEG (SuSoS Technology) was added to the chip surface for 1 h and rinsed three times with Phosphate-Buffered Saline (PBS) ...
-
No products found
because this supplier's products are not listed.
Anthony A. Ruberto, et al.,
bioRxiv - Genomics 2021
Quote:
... primary human hepatocytes (BioIVT) were seeded 2-3 days prior to infection with 15,000-20,000 sporozoites per well ...
-
No products found
because this supplier's products are not listed.
Redouane Aherrahrou, et al.,
bioRxiv - Genetics 2023
Quote:
... Human aortic SMCs (Cell Applications, Inc. ...