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No products found
because this supplier's products are not listed.
Sangeeta Goswami, et al.,
bioRxiv - Immunology 2022
Quote:
... 5X104 GL261 cells were resuspended in 3µl of 70% PBS and 30% Matrigel while 5X104 CT-2A cells were resuspended in 3µl of DMEM media (without FBS) for injection with a stereotactic apparatus (Stoelting) in the cranial window- 2mm posterior and 2mm lateral to the bregma and 3mm deep into the mouse cerebrum.
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No products found
because this supplier's products are not listed.
Nuria Vendrell-Llopis, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Using a rotary micromotor drill (Foredom, Bethel, CT) equipped with a 0.5mm carbon burr (Fine Science Tools, Foster City, CA), a small burr hole was made over the contralateral motor cortex (1.4 mm rostral ...
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No products found
because this supplier's products are not listed.
Francisco J. Martínez-Morcillo, et al.,
bioRxiv - Immunology 2021
Quote:
... were seeded with 105 human foreskin keratinocytes (Ker-CT, ATCC CRL-4048) on the transwells in 300 µL CnT-PR medium (CellnTec) in a 12 well format ...
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No products found
because this supplier's products are not listed.
Jiao Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant human interleukin-12 (IL-2) was gifted from Akron Biotech. Recombinant human interleukin-15 (IL-15 ...
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No products found
because this supplier's products are not listed.
Drew A. Gillett, et al.,
bioRxiv - Immunology 2024
Quote:
Recombinant mouse GPNMB ECD (Aviscera Biosciences, product code 00719-03-10) was diluted in pre-warmed complete RPMI to a final concentration of 0.5ug/ml and 1.0ug/mL and was applied for 4 hours prior to lipopolysaccharide (LPS ...
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No products found
because this supplier's products are not listed.
Alexander Sheh, et al.,
bioRxiv - Microbiology 2020
Quote:
... Hematology analysis was performed by the MIT DCM diagnostic laboratory using a HemaVet 950 veterinary hematology analyzer (Drew Scientific, Oxford, CT). Serum chemistry analysis was performed by Idexx Laboratories (Westbrook ...
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No products found
because this supplier's products are not listed.
Daniel F. Hill, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Briefly, AS4 carbon fibers (Hexel, Stamford, CT) were aspirated into glass capillaries (0.68 mm I.D.; A-M Systems, Inc., Sequim, WA) and a DKI - 700C Vertical Pipette Puller (David Kopf Instruments ...
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No products found
because this supplier's products are not listed.
Jiji Chen, et al.,
bioRxiv - Bioengineering 2020
Quote:
... temperature was set to 37°C using a culture dish heater and temperature control unit (DH-35 and TC-344B, Warner Instruments, Hamden, CT) and an objective heater (Bioptechs, Butler, PA).
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No products found
because this supplier's products are not listed.
Lucas B. Hoffmann, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... 5 µg of sperm DNA from 4 CT and 4 Cort F0 mice were fragmented by centrifugation for 60 s at 7200 rpm using g-TUBES (Covaris, Woburn, MA, USA) to obtain 8 kb-long fragments ...
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No products found
because this supplier's products are not listed.
Sonja Giger, et al.,
bioRxiv - Bioengineering 2021
Quote:
... recombinant human epidermal growth factor (hEGF, 20 ng/ml, Chimerigen Laboratories, CHI-HF-210EGF), recombinant human platelet derived growth factor (hPDGF-AB ...
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No products found
because this supplier's products are not listed.
Joseph de Rutte, et al.,
bioRxiv - Bioengineering 2021
Quote:
... were immunized for a total of 7 times over 28 days with recombinant ovalbumin (Biosearch Technologies) as a model antigen ...
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No products found
because this supplier's products are not listed.
Nandan Haloi, et al.,
bioRxiv - Biophysics 2020
Quote:
... followed by addition of HKII (human recombinant HKII, 60 kU/ml; Genway Biotech, Slan Diego, CA) into the cis chamber ...
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No products found
because this supplier's products are not listed.
Kristin D. Dahl, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... MBP (Myelin Basic Protein, Neuromics Cat # ...
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No products found
because this supplier's products are not listed.
Maya Imbrechts, et al.,
bioRxiv - Immunology 2021
Quote:
... biotinylated recombinant antibodies that were able to bind the antigen were detected with 1/10,000 poly-HRP-conjugated streptavidin (Sanquin) diluted in PTA buffer (100 μL/well ...
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No products found
because this supplier's products are not listed.
Isadora Matias, et al.,
bioRxiv - Neuroscience 2021
Quote:
Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore ...
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No products found
because this supplier's products are not listed.
June-Hyung Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... In vitro ubiquitination of Asc was performed by incubating 300 ng of mouse recombinant Asc (CSB-EP861664MO, CUSABIO, Tx, USA), 1 µg of recombinant Mul1 ...
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No products found
because this supplier's products are not listed.
Jialiang S. Wang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples (60 µl well−1) were then added along with a standard curve of murine recombinant Sclerostin (Alpco, Salem, NH) and plates were incubated overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Priyanka Nain, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... the freshly harvested cells containing recombinant ωTAs were resuspended in Ni-binding buffer enriched with 400 µM PLP (TCI America). Cells were ruptured by sonication and the suspension was centrifuged at 17,100 × g for 1 h in an Eppendorf 5430R centrifuge and the cleared supernatant was further filtered using a 0.22 µm syringe filter (Merck Millipore) ...
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Protein Assay Kit (QCPR-500)
Cat# QCPR-500,
1.0 kit, 500 tests, USD $199.0
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Anne L. Rosen, et al.,
bioRxiv - Immunology 2023
Quote:
... Total protein in the urine was measured using Quantichrom Total Protein Assay Kit (CAS: QTPR-100, BioAssay Systems) according to the manufacturer’s instructions with samples detected at a 1:4-1:20 dilution ...
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No products found
because this supplier's products are not listed.
Eric Waltari, et al.,
bioRxiv - Immunology 2019
Quote:
... Blocking solution (sciBLOCK Protein D1M solution, Scienion) was added at 200 μL/well with a multichannel pipet and allowed to incubate without agitation for 1 hour ...
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No products found
because this supplier's products are not listed.
Li-Juan Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and PRRSV nucleocapsid protein (rabbit monoclonal, VMRD) antibodies were used to localize JEV ...
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No products found
because this supplier's products are not listed.
Chun-Che Tseng, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Instant-Bands Protein Stain (EZBiolab, Parsippany, NJ) were used to visualize input and eluted material on a Typhoon FLA 7000 (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Lennard L. Bohlender, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 10 μg of total protein were mixed with one unit of α-L-arabinofuranosidase from either Aspergillus niger or a corresponding recombinant version (E-AFASE or E-ABFCJ, Megazyme, Bray, Ireland) and incubated over night at 40°C ...
-
No products found
because this supplier's products are not listed.
Qiyue Ding, et al.,
bioRxiv - Biochemistry 2020
Quote:
Protein thiol redox states were monitored using the -SulfoBiotics- Protein Redox State Monitoring Kit Plus (Catalog # SB12; Dojindo Molecular Technologies). Samples were labeled with the Protein-SHifter Plus in accordance with the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Valérie Bougault, et al.,
bioRxiv - Physiology 2021
Quote:
... Serum club cell proteins (cc16) and surfactant proteins D (SP-D) were measured by ELISA (Biovendor, LLC, Candler, NC, USA), according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
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No products found
because this supplier's products are not listed.
Yasunori Watanabe, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein was then stained using methylamine tungstate (Nanoprobes) before being allowed to dry at room temperature for 15 minutes ...
-
No products found
because this supplier's products are not listed.
Qianyan Li, et al.,
bioRxiv - Genetics 2022
Quote:
... and the column was washed with lysis buffer to remove unbound proteins before eluting the proteins with 50 μM biotin (Chem-Impex Int’l) in low salt buffer (20mM HEPES-KOH pH 7.2 ...
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
WNT surrogate-Fc Fusion Protein (Wnt-surrogate; ImmunoPrecise Antibodies Ltd. ...
-
No products found
because this supplier's products are not listed.
Isabelle Leduc, et al.,
bioRxiv - Microbiology 2020
Quote:
... the supernatant mixed with protein A/G resin (ExAlpha Biologicals) for two hours at 4°C with mixing ...
-
No products found
because this supplier's products are not listed.
Joanne F. Garbincius, et al.,
bioRxiv - Physiology 2023
Quote:
... Protein concentration was determined by bicinchoninic acid assay (BioWORLD #20831001). 2500µg of cleared mitochondrial lysate were fractionated by gel filtration using fast protein size-exclusion liquid chromatography (AKTA Pure FPLC ...
-
No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
-
No products found
because this supplier's products are not listed.
Juliane Tschuck, et al.,
bioRxiv - Cell Biology 2022
Quote:
Compounds and proteins for apoptosis and necroptosis assays: Staurosporine (Stauro, TargetMol), Z-VAD-FMK (zVAD ...
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No products found
because this supplier's products are not listed.
Lucia Grenga, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein was subsequently detected with either 1/2000 anti-polyglutamate chain (AdipoGen) or 1/200 anti-RpsF (Dundee Cell Products ...
-
No products found
because this supplier's products are not listed.
Bevin C. English, et al.,
bioRxiv - Immunology 2022
Quote:
... proteins were extracted from samples collected in TRI Reagent (Molecular Research Center) according to a modified protocol (65) ...
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Fluorescent Method
Cat# FPRO200,
FluoProdige fluorescent Dye (10ml) + Dilution Buffer (100ml) + BSA(500µg) lyoph, USD $229.00/KIT
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
-
No products found
because this supplier's products are not listed.
Daniele Merico, et al.,
bioRxiv - Genetics 2019
Quote:
... PVDF membrane was cut at 75 kDa according to protein ladder (BlueElf, FroggaBio). The higher molecular weight portion of the membrane was incubated with ATP7B antibody (Abcam ...
-
No products found
because this supplier's products are not listed.
Kazuma Nakatani, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and the bound proteins were visualized using a chemiluminescence-based detection kit (ImmunoStar Zeta ...
-
No products found
because this supplier's products are not listed.
Yi Xiao Jiang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... or Anti TAR DNA-Binding Protein 43 phospho-Ser409/410 mAb (Cosmo Bio USA, Catalog No ...
-
No products found
because this supplier's products are not listed.
Katherine J. Turner, et al.,
bioRxiv - Neuroscience 2022
Quote:
Rabbit anti-green fluorescent protein (GFP; Torrey Pines Biolabs, Cat# TP401, dilution 1:1000), rat anti-GFP (Nacalai Tesque ...
-
No products found
because this supplier's products are not listed.
Hagit Hak, et al.,
bioRxiv - Plant Biology 2024
Quote:
... and images of protein bands were acquired and quantified using the Alliance Q9 software (UVITEC).
-
No products found
because this supplier's products are not listed.
Shanshan He, et al.,
bioRxiv - Genomics 2022
Quote:
The breast cancer biopsy used for protein assay was CTRL301 tissue microarray supplied by US Biomax, Inc ...
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No products found
because this supplier's products are not listed.
Weigao Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 300 μL pure protein solution was added to a 1 mm pathlength quartz cuvette (Starna Cells, USA). The scanning wavelength starts from 300 nm and ends at 190 nm ...
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No products found
because this supplier's products are not listed.
Amanda L. Peiffer, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Active protein was quantified by titrating in the known active site protease inhibitor FPR-CMK (Haematologic Technologies). To determine the KM the initial fluorescence data ...
-
No products found
because this supplier's products are not listed.
Takanori Eguchi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Overexpressed proteins in the chromatin fraction was analyzed by western blotting using anti-MZF1 antibody (Assay Biotechnology) and anti-SCAND1 antibody (ab64828 ...
-
No products found
because this supplier's products are not listed.
Venkatesh Mallikarjun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Proteins from cell pellets were solubilised by bead-beating using six 1.6 mm steel beads (Next Advance) at 4 °C in 30 µL 1.1% (w/v ...
-
No products found
because this supplier's products are not listed.
Justin Judd, Jonathan Lovas, Guo N. Huang,
bioRxiv - Developmental Biology 2019
Quote:
... Proteins were then transferred to PVDF-Plus membranes (Spectrum Chemical) and blocked in 5% milk in TBST (0.1% Tween 20) ...
-
No products found
because this supplier's products are not listed.
Laure Nicolas Annick Ries, et al.,
bioRxiv - Microbiology 2021
Quote:
Total cellular proteins were extracted as described previously(65) and isocitrate lyase (ICL) activity was measured and calculated as described previously(65) ...
-
No products found
because this supplier's products are not listed.
Karen M. Dunkerley, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Protein phosphorylation was monitored to completion using Phos-Tag AAL™ (NARD Institute Ltd) SDS–PAGE ...
-
No products found
because this supplier's products are not listed.
Catherine S. Millar-Haskell, et al.,
bioRxiv - Bioengineering 2022
Quote:
ECM proteins were covalently attached to PAA using the crosslinker Sulfo-SANPAH (Covachem; 13414-100). To begin ...