-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Matheus F. Sathler, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NIH: HIV-1 IIIB gp120 Recombinant Protein from ImmunoDX, LLC ...
-
No products found
because this supplier's products are not listed.
Thomas Rowe, et al.,
bioRxiv - Microbiology 2024
Quote:
... 180 uL of HEK-λ cells were added to each well containing twenty uL of sample and to serially 1/2-log diluted (0.1 – 1000 ng/mL) recombinant ferret IFNL3 (Kingfisher Biotech, St. Paul, MN, USA). The plates were incubated for 20Hr at 37°C ...
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No products found
because this supplier's products are not listed.
Ahmed Seddek, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Purified recombinant human TOP1 (TopoGEN) was used as positive control.
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Kristoffer Krogerus, Nils Rettberg, Brian Gibson,
bioRxiv - Microbiology 2022
Quote:
... after which spores from the different parental strains were dissected and placed next to each other on YPD agar plates (1 % yeast extract, 2 % peptone, 2 % glucose, and 2 % agar) using a micromanipulator (Singer Instruments, UK). The plates were incubated at 25 °C for 3 days ...
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No products found
because this supplier's products are not listed.
Jeonghwan Youk, et al.,
bioRxiv - Cell Biology 2020
Quote:
... mouse anti-ABCA3 (1:300, Seven Hills Bioreagents, WRAB-ABCA3), and mouse anti-TP63 (1:500 ...
-
No products found
because this supplier's products are not listed.
Hannah L. Bernstein, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Slices were in the chamber for 30 min with sucrose-based ACSF at 1-2 mL/min (#Minpuls 2, Gilson) and then NaCl-based ACSF (125 mM NaCl instead of sucrose ...
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No products found
because this supplier's products are not listed.
Jonathon A.B. Smith, et al.,
bioRxiv - Physiology 2024
Quote:
... and glucose uptake was measured in the same medium by adding 1 uL.mL-1 of 2-[1,2-3H]Deoxy-D-glucose (MT911, Moravek) and 10 μM unlabelled 2-Deoxy-D-glucose in for 15 min [32] ...
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No products found
because this supplier's products are not listed.
Ling Xu, et al.,
bioRxiv - Neuroscience 2021
Quote:
Mice were anesthetized by vaporized isoflurane (induction, 2%; maintenance, 1.5%) and head-fixed in a mouse stereotaxic apparatus (RWD Life Science Co.). A cannula (outside diameter 0.41 mm ...
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No products found
because this supplier's products are not listed.
Charles A. Specht, et al.,
bioRxiv - Immunology 2021
Quote:
... the recombinant proteins were resolved on SDS-PAGE and stained with Coomassie InstantBlue (Expedeon, Ltd.).
-
No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
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No products found
because this supplier's products are not listed.
Caroline Murawski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 2 µm S1818 (Microchem, 5000 rpm, baking at 100°C for 1 min). Patterns were developed for 40 – 50 s in MF319 (Microchem) ...
-
No products found
because this supplier's products are not listed.
Chengyao Wang, et al.,
bioRxiv - Bioengineering 2020
Quote:
Human intestinal epithelial cells Caco-2 (ATCC® HTB-37™) were cultured in a 75 cm2 cell culture flask (Nest Biotechnology) with complete 1X Eagle’s Modified Eagle Medium (EMEM ...
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No products found
because this supplier's products are not listed.
Zhimin Liu, et al.,
bioRxiv - Systems Biology 2020
Quote:
... ∼7.4 × 109 of frozen cells were inoculated into 1.2 L media in a 2 L Delong flask (Bellco). The cells were grown for a total of 21 generations ...
-
No products found
because this supplier's products are not listed.
Franziska Blaeschke, et al.,
bioRxiv - Immunology 2022
Quote:
... T cells were incubated with NY-ESO-1 specific dextramer (Immudex) for 12 min at RT (1:50 dilution) ...
-
No products found
because this supplier's products are not listed.
Pallab Pradhan, et al.,
bioRxiv - Immunology 2020
Quote:
... Anti-CD3/CD28 bead (Dynabeads)-activated PBMCs (MSC/PBMC at 1:2) from healthy volunteers (purchased from Zen-Bio) plated in 200 ul culture media (RPMI 1640 + 10% FBS + 1% PS+ 30 IU/mL IL2 ...
-
No products found
because this supplier's products are not listed.
Maritza Oñate, et al.,
bioRxiv - Neuroscience 2019
Quote:
... brain sections were heated at 80°C for 30 min in 50mM citrate buffer pH 6.0 for antigen retrieval prior overnight incubation with anti-phospho-MLKL (S358) mouse monoclonal antibody (1:200: Signalway antibody). Primary antibody was detected by incubating 1 hour with sheep anti-mouse HRP-linked secondary antibody (General Electric) ...
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No products found
because this supplier's products are not listed.
Ujjayini Ghosh, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and then incubated with 20 µl of a 1 mM solution of azide-PEG-maleimide and azide-PEG-methoxyl (5 and 2 kDa molecular weight, respectively, Nanocs) at a 1:9 ratio for 6 hours ...
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No products found
because this supplier's products are not listed.
Yeranuhi Hovhannisyan, et al.,
bioRxiv - Pathology 2023
Quote:
... and CW30318 (Control 2, Fuji Cellular Dynamics) derived from healthy individuals without any cardiac pathology ...
-
No products found
because this supplier's products are not listed.
Bee Hui Liu, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... varying concentrations of compounds 1-80 were added to cells with multichannel electronic pipettes (Rainin). Cells were then incubated for 72 hrs at 37°C in a humidified atmosphere of 5% CO2 before 10 μl of CellTiter-Glo reagent was added to the wells with the MultiFlo Microplate Dispenser (BioTek) ...
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No products found
because this supplier's products are not listed.
M. Chighizola, et al.,
bioRxiv - Biophysics 2020
Quote:
... and plated in low concentration of 4.000 cells/cm2 (to guarantee the presence of single separated cells) on Ø 40 mm glass-bottom dishes for cell culture (Willco Wells) the day before the experiment ...
-
No products found
because this supplier's products are not listed.
Alan Shaw, et al.,
bioRxiv - Biophysics 2023
Quote:
... Ligand for Broccoli RNA aptamer BI (LuceRNA) was prepared in 50mM DMSO and further diluted in water ...
-
No products found
because this supplier's products are not listed.
Natasha Ting Lee, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant mouse t-PA (0.9 mg/kg; Molecular Innovations, MI, USA) or saline (equi-volume ...
-
No products found
because this supplier's products are not listed.
June-Hyung Kim, et al.,
bioRxiv - Immunology 2019
Quote:
... In vitro ubiquitination of Asc was performed by incubating 300 ng of mouse recombinant Asc (CSB-EP861664MO, CUSABIO, Tx, USA), 1 µg of recombinant Mul1 ...
-
No products found
because this supplier's products are not listed.
Mitchell R. Lewis, Tara L. Deans,
bioRxiv - Synthetic Biology 2023
Quote:
Mouse embryonic stem cells (harvested from a TARGATT mouse, Applied StemCell) (see Note 1).
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No products found
because this supplier's products are not listed.
Hyunjoon Kim, Soohyun Jang, Young-suk Lee,
bioRxiv - Molecular Biology 2021
Quote:
... cells were selected with medium containing 1∼2 μg/ml puromycin (AG Scientific #P-1033) until non-transduced cells became completely dead ...
-
No products found
because this supplier's products are not listed.
Shiri Levy, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 25 ng/ml human recombinant FGF4 (Reliatech), 2 ng/ml human recombinant TGF-ß1 (PeproTech) ...
-
No products found
because this supplier's products are not listed.
Uriel López-Sánchez, et al.,
bioRxiv - Biochemistry 2024
Quote:
... All ligands were dissolved in frogs Ringer’s solution and applied using an automated programmable perfusion system (Bioscience Tools). Desensitization rate constants (τdes ...
-
No products found
because this supplier's products are not listed.
Jan-Renier A.J. Moonen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Flag-tagged KLF4 (#VH829440, Vigene Biosciences) or GFP control (AVP004, GenTarget Inc) for 12 h after which cells were allowed to recover for 90 h before being harvested for subsequent experiments.
-
No products found
because this supplier's products are not listed.
Chetan Kumar Arya, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and Wizard Classic 1 and 2 (Rigaku Japan). Crystals of DMFase were obtained by mixing 200 nl of protein in buffer A with equal volumes of precipitant ...
-
No products found
because this supplier's products are not listed.
Mari Akiyma,
bioRxiv - Cell Biology 2020
Quote:
... Alkaline phosphatase-tagged antibody was visualized with PermaRed/AP (K049, Diagnostic BioSystems, CA, USA). Incubation with mouse monoclonal antibodies for osteocalcin (diluted 1:100 ...
-
No products found
because this supplier's products are not listed.
Xiaowei Sun, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Recombinant human insulin (M9194) was purchased from AbMole (Houston, USA). The ARF1 inhibitor Golgicide A (HY-100540 ...
-
No products found
because this supplier's products are not listed.
Atanas Radkov, et al.,
bioRxiv - Biochemistry 2022
Quote:
... PG ligand was dialyzed against water using a float dialysis membrane device with a 100-500 Da cut-off (Spectrum Laboratories, Inc) and lyophilized before preparation of a concentrated stock solution in NMR buffer ...
-
No products found
because this supplier's products are not listed.
Veena Padmanaban, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... mouse anti-dsRNA (1:200, Axxora, JBS-RNT-SCI-10010200) and phalloidin (1:200 ...
-
No products found
because this supplier's products are not listed.
Shuo Yang, et al.,
bioRxiv - Microbiology 2022
Quote:
... anti-SARS-CoV-2-ORF3a (1:250; 101AP, FabGennix International Inc), anti-Actin (1:500 ...
-
No products found
because this supplier's products are not listed.
Lucia Leitner, et al.,
bioRxiv - Developmental Biology 2024
Quote:
Total RNA was extracted from single cell suspensions of 500,000 CSCs or CB-derived cells using the FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit (Favorgen, FABRK001-2), eluted with 40μl RNase-free ddH2O and the RNA concentration was measured with a Nanodrop 2000c spectrophotometer (Thermo Scientific) ...
-
PDCD1LG2 is a recombinant protein.
Cat# abx261348-25UG,
25 µg USD $377.0
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Isabel Brandão, et al.,
bioRxiv - Physiology 2023
Quote:
... or after the primary antibody was incubated with recombinant Human Peroxidasin Homolog Protein (Abbexa; catalog # abx068474), in equimolar (1:1 ...
-
No products found
because this supplier's products are not listed.
Eric Vallabh Minikel, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Samples were injected (1 to 2 µg) onto a 75 um ID PicoFrit column (New Objective) packed to 20 cm with Reprosil-Pur C18 AQ 1.9 um media (Dr ...
-
No products found
because this supplier's products are not listed.
Pinja Kettunen, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 2 μM Xav939 (BioGems).
-
No products found
because this supplier's products are not listed.
Simone Kurz, et al.,
bioRxiv - Biochemistry 2020
Quote:
... mouse brain (Pel-Freez Biologicals), or HEK293 cells (kind gift from Dr ...
-
No products found
because this supplier's products are not listed.
Annelien Morlion, et al.,
bioRxiv - Genomics 2021
Quote:
... gDNA heat-and-run removal was performed by adding 1 μl HL-dsDNase (ArcticZymes #70800-202, 2 U/μl) and 0.68 μl reaction buffer (ArcticZymes #66001 ...
-
No products found
because this supplier's products are not listed.
Marta Bermejo-Jambrina, et al.,
bioRxiv - Immunology 2023
Quote:
... SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
-
No products found
because this supplier's products are not listed.
Chao Wang, et al.,
bioRxiv - Cell Biology 2019
Quote:
... anti-mouse-HRP antibody (ImmunoVision Technologies) was added and the cells were incubated with the secondary antibody for 2 hrs ...
-
No products found
because this supplier's products are not listed.
B. van de Kooij, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... was used for MDA-MB-436 and mouse targeting shEXO1 (see Table 1 for sequence, cloned in pRSITEP-U6Tet-sh-EF1-TetRep-2A-Puro from Cellecta Catalog #: SVSHU6T16-L) was used for MEFs.
-
No products found
because this supplier's products are not listed.
Jutamas Uttagomol, et al.,
bioRxiv - Cell Biology 2019
Quote:
... cells were plated and grown for 1∼2 days on collagen-coated BioFlex 6-well culture plates with flexible silicone elastomer bottoms (BF-3001C, Flexcell® International Corporation). Each plate was placed over the loading station containing 6 planar faced posts ...
-
No products found
because this supplier's products are not listed.
Lijun Cong, et al.,
bioRxiv - Microbiology 2021
Quote:
... prior to cell supernatants being collected for quantification of virus production by HIV-1 p24 ELISA (XpressBio).
-
No products found
because this supplier's products are not listed.
John B. McManus, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... Reactions were distributed by electronic pipette and TetR dilutions were distributed into cell-free reactions in volumes of 1 µL using an Echo 525 acoustic liquid handler (Labcyte Inc.).
-
No products found
because this supplier's products are not listed.
Shunsuke Kataoka, et al.,
bioRxiv - Immunology 2021
Quote:
... and then pulsed with 2 μg/ml staphylococcal enterotoxin E (SEE) (Toxin Technology #ET404) for 1hr at 37°C ...
-
No products found
because this supplier's products are not listed.
Liz M. Florez, et al.,
bioRxiv - Plant Biology 2020
Quote:
... PCR amplification products were visualised following gel electrophoresis in a 2% (w/v) agarose gel in 1x Tris Acetate-EDTA (TAE) buffer with RedSafe™ (Intron Biotechnology, SEL, Korea), at the manufacturer’s recommended concentration ...