-
No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
-
No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Samuele Cancellieri, et al.,
bioRxiv - Genetics 2021
Quote:
... and 100 ng ml-1 recombinant human FMS-like Tyrosine Kinase 3 Ligand (Flt3-L) (CellGenix cat# 1415-050). HSPCs were electroporated with 3xNLS-SpCas9:sg1617 RNP or HiFi-3xNLS-SpCas9:sg1617 RNP 24 h after thawing ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
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No products found
because this supplier's products are not listed.
Lamar S. Thomas, Laura C. Cook,
bioRxiv - Microbiology 2022
Quote:
... The Tecan HP Infinite 200 Pro spectrophotometer was programmed by Tecan i-control software for incubation at 37°C and measurement of OD600 every 30 minutes with shaking for 10 secs prior to measurement ...
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No products found
because this supplier's products are not listed.
Bastijn J.G. van den Boom, et al.,
bioRxiv - Neuroscience 2022
Quote:
... DBS parameters were programmed in a digital stimulator (DS8000, WPI, USA) and generated by isolators (DLS100 ...
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No products found
because this supplier's products are not listed.
Yu Rebecca Miao, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and mouse anti-HIS Hilyte Fluor 488 (Anaspec).
-
No products found
because this supplier's products are not listed.
Priyadharshini Devarajan, et al.,
bioRxiv - Immunology 2022
Quote:
... and recombinant Mouse IL-15 (Tonbo Biosciences) were suspended and mixed in PBS ...
-
No products found
because this supplier's products are not listed.
Denise Jahn, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant mouse Cgrp (Bachem #H-2265, 10-7M).
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No products found
because this supplier's products are not listed.
Ramakanth Neeli-Venkata, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cell death assays were performed on a wide-field fluorescence microscope (TI-Eclipse; Nikon) operated with Micro-Manager (Open Imaging) ...
-
No products found
because this supplier's products are not listed.
Christopher D. Greer, et al.,
bioRxiv - Immunology 2021
Quote:
Mouse IL-2 (MABTECH, 3441-4APW-2), IL-4 (MABTECH ...
-
No products found
because this supplier's products are not listed.
Waipan Chan, et al.,
bioRxiv - Genomics 2020
Quote:
BMDM or RAW cells were stimulated with TLR4 ligand Kdo2-Lipid A (KLA, Avanti Polar Lipids). Ligand addition was staggered so that for all time points ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Quynh T. Phan, et al.,
bioRxiv - Microbiology 2021
Quote:
... mouse liver endothelial cells (Cell Biologics), and hTert-immortalized human microvascular endothelial cells (American Type Culture Collection ...
-
No products found
because this supplier's products are not listed.
Amit Bachar, et al.,
bioRxiv - Microbiology 2019
Quote:
... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
K. Melnattur, B. Zhang, P.J. Shaw,
bioRxiv - Neuroscience 2019
Quote:
... mouse anti TH (1:500, Immunostar), mAb nc82 (1:400 ...
-
No products found
because this supplier's products are not listed.
Arun Prasath Damodaran, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... mouse anti-His tag (HIS.H8 / EH158, Covalab, 1:2500), mouse anti-FLAG tag (clone M2-F1804 ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... Cells were then treated with 10 ng/mL recombinant mouse TNFα (Cell Applications, cat# RP2031-20) for 45 min in basal Endothelial Cell Growth Media MV2 (PromoCell ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and human recombinant bFGF-2 (10ng/mL; ABM). DMEM-F12 w/o L-Glutamine w/o Hepes w/o Glucose (Biowest ...
-
No products found
because this supplier's products are not listed.
Gabriel Ozorowski, et al.,
bioRxiv - Biophysics 2020
Quote:
The ChemBridge ligand library (1.3M compounds [https://www.chembridge.com/] (accessed February 2019)] ...
-
No products found
because this supplier's products are not listed.
Shutang Tan, et al.,
bioRxiv - Plant Biology 2019
Quote:
... membranes were incubated for 2 h at room temperature with an anti-His HRP-(horseradish peroxidase) conjugated antibody (1:4000; Agrisera) or an anti-GST HRP-conjugated antibody (1:4000 ...
-
No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... neurons were seeded with 10 µM recombinant Aβ42 fibrils (rPeptide A-1163-2). Preformed fibrils were sonicated for ten minutes in a water bath sonicator system before seeding ...
-
No products found
because this supplier's products are not listed.
Albert Chun Shuo Huang, et al.,
bioRxiv - Immunology 2022
Quote:
... and anti-receptor activator of nuclear factor-kappa B ligand (RANKL) (dilution ratio: 1:400) (Bioss, Woburn, MA). After deparaffinization and rehydration ...
-
No products found
because this supplier's products are not listed.
Callum J. Fraser, et al.,
bioRxiv - Microbiology 2022
Quote:
1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
-
No products found
because this supplier's products are not listed.
Yinghui Li, et al.,
bioRxiv - Pathology 2022
Quote:
... and cell death (blue coloration) were examined in the leaf tissues under a stereo microscope Zeiss Axio Imager M2 (Carl Zeiss, Germany). All microscopic experiments were repeated thrice ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
-
No products found
because this supplier's products are not listed.
Chop Yan Lee, et al.,
bioRxiv - Systems Biology 2023
Quote:
... For each passage 1-2×106 cells were seeded in T25 flasks (Sarstedt). Then ...
-
No products found
because this supplier's products are not listed.
Jiah Kim, Nimish Khanna, Andrew S. Belmont,
bioRxiv - Cell Biology 2019
Quote:
Cells were plated on 35mm dishes with a #1 1/2 thickness glass coverslip bottom (MatTek, P35G-1.5-14-C) 48 hrs before imaging ...
-
No products found
because this supplier's products are not listed.
Senthilvelrajan Kaniyappan, et al.,
bioRxiv - Neuroscience 2020
Quote:
... covered with either 2 nm amorphous carbon (Quantifoil, R2/1+2 nm C) or graphene were used for sample preparation ...
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Michael S. Balzer, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 10 U/ml recombinant mouse interferon-□ (Cell Sciences, Canton, MA) at 33 °C (permissive conditions ...
-
No products found
because this supplier's products are not listed.
Huilei Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... An adenovirus used to overexpress C-terminal His-tagged LOXL2 was purchased from Vector Biolabs. Vitamin E (α-Tocopherol ...
-
No products found
because this supplier's products are not listed.
Désirée König, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Mouse anti-desmoplakin 1/2 (1:50; Progen); Rabbit anti-tp63 (1:500 ...
-
No products found
because this supplier's products are not listed.
Tia A. Tummino, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
Ligands were sourced from Enamine (‘4042) or Sigma-Aldrich (CP-55,940 ...
-
No products found
because this supplier's products are not listed.
Yue Li, Edmund Hollis II,
bioRxiv - Neuroscience 2021
Quote:
... the DREADD ligand JHU37160 (J60, Hello Bio) was administered via intraperitoneal injection at a dose of 1.0 mg/kg 30 minutes prior to rotarod training.
-
No products found
because this supplier's products are not listed.
Harry C. Tjondro, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Degranulated MPO (Dg-MPO) and cell death were monitored longitudinally in supernatants using ELISA (ICL LAB) and lactate dehydrogenase (LDH ...
-
No products found
because this supplier's products are not listed.
Mohamed Ibrahim, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and Ligand-Friendly Screen (LFS) from Molecular Dimensions, were used for crystallization using a Crystal Phoenix robot (Art Robbins) ...
-
Denosumab (anti-RANK ligand) (AMG-162, Prolia, Xgeva) is a fully human monoclonal antibody that...
Cat# A2020, SKU# A2020-1mg,
1mg, $239.00
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Jonuelle Acosta, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Drugs or cell death inhibitors are used at the following concentrations: Necrostatin-1s (10μg/mL, Selleck Chemicals), Ferrostatin-1 (2μM ...
-
No products found
because this supplier's products are not listed.
Nitin Hingankar, et al.,
bioRxiv - Immunology 2021
Quote:
... The avi-tagged SARS-CoV-2 RBD antigen was first labeled with biotin (Avidity, BirA500) was subsequently coupled to streptavidin-PE and streptavidin-APC (BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Romain Durand-de Cuttoli, et al.,
bioRxiv - Animal Behavior and Cognition 2023
Quote:
... Rewards were delivered using a custom-built 3D-printed automated pellet dispenser that was triggered by a computer running the task programmed in ANY-Maze (Stoelting), run under dim lighting.
-
12 well plate with tissue culture treated #1.5 glass-like polymer cover slip (0.175±0.010mm)....
Cat# P12-1.5P,
20/case, $221.00
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Minghui Di, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HeLa cells were transfected with DNA (1 ug) expressing mCherry-tagged proteins cultured on 29-mm glass-bottom dish (Cellvis); plasmids (1 ug ...
-
No products found
because this supplier's products are not listed.
Anniek Stokkermans, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... Recombinant Cas9 protein (900 ng μl−1; PNA Bio, #CP01-20) was co-injected with both sgRNAs (20μM each ...
-
No products found
because this supplier's products are not listed.
Albert Herms, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant R-Spondin 1 protein (500 ng/m, AMSBIO, AMS.RS1-H4221), recombinant Noggin protein (0.1 μg/ml ...
-
No products found
because this supplier's products are not listed.
Abishek Chandrashekar, et al.,
bioRxiv - Microbiology 2022
Quote:
... followed by mouse Mach-2 HRP-Polymer (Biocare) for 30 min and then counterstained with hematoxylin followed by bluing using 0.25% ammonia water ...
-
No products found
because this supplier's products are not listed.
Mariana F. Tioni, et al.,
bioRxiv - Immunology 2021
Quote:
Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 1% fibroblasts growth supplement-2 (FGS-2, 2382, ScienCell) and 1% penicillin-streptomycin (P/S ...
-
No products found
because this supplier's products are not listed.
Joonyong Lee, et al.,
bioRxiv - Physiology 2021
Quote:
... Serum IGF2 levels were quantified using a 1/6 dilution with the Mouse IGF-2 ELISA Kit (Boster Biological Technology, EK0381) as per directions by the manufacturer and read with the SpectraMax M2e spectrophotometer using the SoftMax Pro 6 program.