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No products found
because this supplier's products are not listed.
Moumita Sarkar, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... pCDNA-T292A/S294A and pCDNA-Cnot3 Δ651-751 constructs were in vitro transcribed/translated as described above and the resultant V5 tagged proteins were purified using V5-tagged protein purification kit ver.2 (MBL International, USA). Purified proteins were used as substrate and incubated with 80 ng of GST-Aurora B (ProQuinase ...
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No products found
Daniel M. Sapozhnikov, Moshe Szyf,
bioRxiv - Molecular Biology 2020
Quote:
... and dCas9 recombinant protein (Applied Biological Materials Inc.). After 10 minutes at room temperature ...
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No products found
because this supplier's products are not listed.
Kanak Raina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
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No products found
because this supplier's products are not listed.
Yoji Tsugawa, et al.,
bioRxiv - Microbiology 2024
Quote:
... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
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No products found
because this supplier's products are not listed.
Hsuan Cheng, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The intensity of YFP-FKBP-tagged proteins at centrosomes upon rapamycin treatment was measured by Nikon Elements AR software.
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No products found
because this supplier's products are not listed.
Linda J. Olson, et al.,
bioRxiv - Biochemistry 2020
Quote:
... an Avi tag and XbaI and used to generate Baculovirus using the BestBac method (Expression Systems). Spodoptera frugiperda (Sf9 ...
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No products found
because this supplier's products are not listed.
Minee L. Choi, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and biotinylated cardiolipin (Echelon Biosciences) were obtained as a lyophilized powder ...
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No products found
because this supplier's products are not listed.
Izumi Kurata-Sato, et al.,
bioRxiv - Immunology 2023
Quote:
... biotinylated NP-BSA (Biosearch Technologies) was incubated with APC-conjugated streptavidin (BioLegend ...
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No products found
because this supplier's products are not listed.
Yue Zhu, Seyit Yuzuak, Xiaoyan Sun, De-Yu Xie,
bioRxiv - Plant Biology 2023
Quote:
The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
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No products found
because this supplier's products are not listed.
Ella F.J. Halcrow, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HeLa cells expressing the different GFP-tagged proteins were plated on an open μ-Slide with 8 wells (Ibidi, 80826) in complete medium containing 0.5 μM SiR-DNA dye (SpiroChrome) ...
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No products found
because this supplier's products are not listed.
Miriam R. Fein, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Fc receptor blocker (Innovex Biosciences), and finally avidin/biotin blocking buffer (Vector Laboratories ...
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No products found
because this supplier's products are not listed.
Robin Hoeven, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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No products found
because this supplier's products are not listed.
Neil G. Rumachik, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... FRG mice were maintained on irradiated high-fat low-protein mouse chow (Envigo Teklad Global 16% Protein Cat#2916) ad libitum to decrease flux through the tyrosine pathway ...
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No products found
because this supplier's products are not listed.
Kazuki Hattori, et al.,
bioRxiv - Bioengineering 2022
Quote:
... The device was presoaked in FC-40 (3M) for more than 30 min and placed in a stage top incubator (STXG-WSKMX ...
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No products found
because this supplier's products are not listed.
Danila Boytsov, et al.,
bioRxiv - Biophysics 2022
Quote:
... the FCS unit (Confocor3, Carl Zeiss, Jena, Germany) of a commercial laser scanning microscope (LSM 510 ...
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No products found
because this supplier's products are not listed.
Wanqiu Ding, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
Total protein was extracted from the mouse brain using RIPA lysis buffer (Solarbio R0020) supplemented with 1 mM PMSF and complete protease inhibitor cocktail ...
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No products found
because this supplier's products are not listed.
Florian Noack, et al.,
bioRxiv - Genomics 2020
Quote:
... sheared and biotinylated fully methylated pUC19 (Zymo Research, Cat. N: D5017) and unmethylated lambda DNA (Promega ...
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No products found
because this supplier's products are not listed.
Tyler B. Waltz, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant rat protein p11 (S100A10 Recombinant Protein, Aviva Systems Biology, OPCD06771) was dissolved in distilled water to obtain a final concentration of 100ug/mL and stored at -80C for less than one month ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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No products found
because this supplier's products are not listed.
Hiroshi Ichise, et al.,
bioRxiv - Immunology 2021
Quote:
... Recombinant mouse thrombin was obtained from antibodies-online GmbH (Aachen ...
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No products found
because this supplier's products are not listed.
Denise Jahn, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant mouse Cgrp (Bachem #H-2265, 10-7M).
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No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
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No products found
because this supplier's products are not listed.
Yi-Nan Zhang, et al.,
bioRxiv - Immunology 2021
Quote:
... Recombinant mouse Flt3 ligand (Flt3L) and mouse SCF were purchased from Shenandoah Biotech (Warwick, PA). Cells were stained with appropriate concentrations of mAbs ...
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No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
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No products found
because this supplier's products are not listed.
Jennifer H. Kong, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Biotinylated proteins from clarified supernatants were captured on a streptavidin agarose resin (TriLink Biotechnologies), washed once with Biotinylation Lysis Buffer A ...
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No products found
because this supplier's products are not listed.
Temitayo T. Bamgbose, et al.,
bioRxiv - Immunology 2023
Quote:
... and treated with recombinant mouse IL-4 (Leinco technologies, Inc. I-207) at a 40ng/ml concentration for 6 hours ...
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No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Fc receptors were blocked for 30 min with Protein A (MP Biomedicals, OH) in Stain Buffer (554656 ...
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No products found
because this supplier's products are not listed.
Francois Chesnais, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) were cultured on fibronectin-coated (PromoCell) plates up to passage 7 and maintained in Endothelial Growth Medium 2 (EGM2 ...
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No products found
because this supplier's products are not listed.
Luisa Diomede, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... FLAG-tagged ACE2 (AdipoGen) was captured on the chip by a previously immobilized anti-FLAG antibody (Merck Life Science S.r.l) ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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Recombinant protein produced in Pichia pastoris. Free of animal derived components, RNases, and...
Cat# LS006361,
10 ku, $200.00
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Meiling Zhang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
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No products found
because this supplier's products are not listed.
Yang Xu, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
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No products found
because this supplier's products are not listed.
Erica R. Vander Mause, et al.,
bioRxiv - Bioengineering 2022
Quote:
... a C-terminal AviTag was added to the pSANG10 expression constructs (pSANG10-Avi) and scFvs transformed into BL21[DE3] cells (Lucigen) containing the pBirAcm plasmid (Avidity).
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No products found
because this supplier's products are not listed.
Logan K. Smith, Kareem Fawaz, Bebhinn Treanor,
bioRxiv - Immunology 2020
Quote:
... were mixed with 2.5 ×10−4% biotinylated liposomes (N-Biotinylated Cap PE: Avanti Polar Lipids, Inc.). Alexa Fluor 633-streptavidin (1:1000 v/v ...
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No products found
because this supplier's products are not listed.
Xiaoling Qiang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant proteins were tested for LPS content by the chromogenic Limulus amebocyte lysate assay (Endochrome; Charles River), and the endotoxin content was less than 0.01 U per microgram of recombinant proteins.
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No products found
because this supplier's products are not listed.
Pablo Lazcano, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Corresponding europium-tagged secondary antibodies (Molecular Devices) were used at a 1:5000 dilution and 1:20,000 for actin ...
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No products found
because this supplier's products are not listed.
Yashwanth Ashok, et al.,
bioRxiv - Microbiology 2019
Quote:
... contained 50 nM rPtxS1 proteins and 500 nM rGαi in 50 mM sodium phosphate (pH 7.0) and 1 μM biotinylated NAD+ (Trevigen, 4670-500-01). The reactions were carried at room temperature for 40 minutes ...
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No products found
because this supplier's products are not listed.
Peng Dong, et al.,
bioRxiv - Genomics 2022
Quote:
... Mouse embryonic stem cells were plated on Human recombinant laminin 511-coated coverslips (Electron Microscopy Sciences, Cat. 72196-25). Cells were then fixed using 4% formaldehyde (Thermo Fisher Scientific ...
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No products found
because this supplier's products are not listed.
Megha Subramanian, et al.,
bioRxiv - Genomics 2022
Quote:
... TTR protein levels were measured with mouse prealbumin kit from ALPCO, 41-PALMS-E01 (serum diluted 1:4000) ...
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No products found
because this supplier's products are not listed.
Iwona M. Pranke, et al.,
bioRxiv - Cell Biology 2022
Quote:
... proteins were detected with appropriate antibodies: K8 – mouse monoclonal ab (61038, Progen), Sel1 (sc-377350 ...
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No products found
because this supplier's products are not listed.
Rosa Pipitone, et al.,
bioRxiv - Plant Biology 2020
Quote:
... and PsaC was performed according to Agrisera instructions and using recombinant proteins (PsbA AS01 0116S, PetC AS08 330S, and PsaC AS04 042S; Agrisera, Vännäs, SWEDEN). Three respective calibration curves for the three recombinant proteins were created ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
A. Louise Hunter, et al.,
bioRxiv - Physiology 2020
Quote:
... Here the raw data was searched against the mouse Swissprot database (release October 2017) using the paragon algorithm on Protein-Pilot (Version 5.0.1, AB SCIEX). A total of 33847 proteins were searched ...
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No products found
because this supplier's products are not listed.
Mohammad Saeed, et al.,
bioRxiv - Immunology 2022
Quote:
... Fetal calf serum (FCS) and antibiotics were obtained from American Type Culture Collection (ATCC). Trypan blue (Life technologies ...
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No products found
because this supplier's products are not listed.
Peixiang Zhang, et al.,
bioRxiv - Physiology 2022
Quote:
... mice were maintained on mouse chow (diet D1001 containing 10 kcal% fat, 20 kcal% protein and 70 kcal% carbohydrate; Research Diets, New Brunswick, NJ) or chow containing simvastatin (0.1 g/Kg body weight in mouse chow ...
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No products found
because this supplier's products are not listed.
Mitsugu Shimobayashi, et al.,
bioRxiv - Physiology 2020
Quote:
Plasma Leptin levels were measured by mouse mouse Leptin ELISA kit (Crystal Chem) according to the manufacturer’s instructions.