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Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged
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Creative Biomart Sponsored
Recombinant Mouse Fzd1 extracellular domain (Met 1-His 248) (NP_067432.2), fused with a...Cat# Fzd1-2292M, 50.0 µg, USD $429.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular Biology of the Cell doi: 10.1091/mbc.E19-07-0362Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.02328Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: The following His-tagged recombinant proteins were purchased from Sino Biologicals: B.1.1.7 rS1-SARS-CoV-2 spike S1 [Δ 69-70 ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in ACS Infectious Diseases doi: 10.1021/acsinfecdis.9b00412Quote: ... BSA (24 - 48 h at 4°C in rotation) for HIS-tagged rPtxS1 and rGαi proteins with mouse monoclonal anti-HIS (1:1000) (R&D Systems, MAB050), for Gαi with mouse monoclonal anti-Gαi (1:500 ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
abcam
No products found because this supplier's products are not listed.Cited in The complement system supports normal postnatal development and gonadal function in both sexesbioRxiv - Developmental Biology 2017Quote: ... rb α-mouse vasa homolog (MVH) (Abcam 13840, 1:1,000), donkey α −gt CF594 (Biotium ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018Quote: Recombinant mouse TNF was from Peprotech and recombinant mouse IL-3 was from R&D ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01905-18Quote: ... His-tagged proteins were detected with mouse anti-His tag (27E8) antibody at a dilution of 1:1000 (Cell Signaling, Danvers, MA) and an anti-mouse IgG HRP linked antibody at a dilution of 1:2000 (Cell Signaling ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Nature Chemical Biology doi: 10.1038/s41589-019-0304-8Quote: Recombinant Flag-tagged RNF114 was used as bait to precipitate pure recombinant p21 (Origene Technologies Inc. ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in mAbs doi: 10.1080/19420862.2020.1714371Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in Nature Communications doi: 10.1038/s41467-018-07251-5Quote: ... His-tagged proteins were immobilized on Ni-NTA (Merck Millipore), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019Quote: ... and sonic hedgehog homolog (Shh; 1:500, 20697-1-AP; Proteintech) overnight at 4°C ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018Quote: The recombinant protein His-Syncytin-1 was expressed in Escherichia coli BL21-codonplus (DE3)-RIPL competent cells (Agilent Technologies), according to standard protocol ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... His-tagged RanQ69L (4 mM) was preincubated on ice with MagnetHis™ Ni-Particles (Promega) in phosphate binding buffer with 150 mM NaCl and 3 mM β-mercaptoethanol for 30 mins ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018, published in The Plant Cell doi: 10.1105/tpc.19.00150Quote: ... the following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:600; Santa Cruz Biotechnology); mouse monoclonal anti-HA clone HA-7 (1:3000 ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
Active Motif
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019Quote: ... Soluble mouse recombinant IL10 and IFNγ were procured from BD Biosciences (San Diego ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2017, published in Neuropsychopharmacology doi: 10.1038/s41386-018-0088-7Quote: ... followed by Cy3-tagged donkey antimouse antibody (1:500; Jackson ImmunoResearch). EYFP positive cells were identified using chicken anti-EGFP antibody (1:1000 ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-020-0380-1Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018Quote: ... and (d) FITC tagged anti-mouse antibody (1:200 dilution; Vector Labs). After each incubation ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ... -
Abnova
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Journal of Cell Biology doi: 10.1083/jcb.201806195Quote: ... diluted 1:1 in PBS for 1 hour at RT and probed with rabbit-His (MBL, 1:1000) and mouse-KBP (Abnova, 1:1000) overnight at 4°C ... -
Genetex
No products found because this supplier's products are not listed.Cited in “Hemicellulose Degradation and Utilization by a Synthetic Saccharomyces cerevisiae Consortium”bioRxiv - Bioengineering 2018Quote: ... were pelleted then re-suspended in 250 μL of PBS containing 1mg/mL bovine serum albumin and 0.5 μg of 1°-Anti-6X His tag mouse antibody (Genetex Inc., CA, USA) for 4 h with occasional mixing ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Genomics 2019, published in G3: Genes|Genomes|Genetics doi: 10.1534/g3.119.400922Quote: Raw Nanopore and Hi-C (Illumina) reads have been deposited in the NCBI SRA and are under the BioProject (PRJNA565796) ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular and Cellular Biology doi: 10.1128/MCB.00158-19Quote: ... and recombinant mouse M-CSF (Calbiochem) at a final concentration of 10ng/mL for seven days ... -
LI-COR
No products found because this supplier's products are not listed.Cited in Secretion of an Argonaute protein by a parasitic nematode and the evolution of its siRNA guidesbioRxiv - Molecular Biology 2019, published in Nucleic Acids Research doi: 10.1093/nar/gkz142Quote: ... Quantification was carried by western blot analysis in comparison to known concentrations of recombinant full length exWAGO containing an N-terminal 3XFlag-His tag using Image Studio Lite (LI-COR) software ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018, published in PLOS Genetics doi: 10.1371/journal.pgen.1007658Quote: Mice carrying the murine homolog of the FVL mutation [29] (F5L; also available from Jackson Laboratories stock #004080) or the TFPI Kunitz domain deletion (Tfpi- ... -
Synaptic Systems
No products found because this supplier's products are not listed.Cited in Neuroligins mediate presynaptic maturation through brain-derived neurotrophic factor signalingbioRxiv - Neuroscience 2018Quote: ... mouse anti Synaptotagmin-1 (Synaptic Systems) 1:200 ... -
Biovision
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... 100 µl of mouse anti-His-tag antibody (1:1000, BioVision, USA) was incubated at 37□ for 2 h ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ... -
Rockland
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Experimental Biology and Medicine doi: 10.1177/1535370219862282Quote: Primary antibodies used in Drosophila cells were: anti-γH2Av (1:1000, Rockland, 600-401-914); anti-GFP (1:1000 Aves Lab ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... and RNF34-HIS (Novus Biologicals) fusion proteins in a total volume of 30μl ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... subsequently diluted in water (1:4) and mixed with SensiFAST SYBR Hi-Rox (Bioline) and the appropriate primers at a final concentration of 10 μM ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007848Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ... -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... GFP tagged proteins was detected with GFP-booster coupled with Atto488 (1:500; Chromotek). DNA was stained by DAPI (0.2 µg/ml ... -
SignalChem
No products found because this supplier's products are not listed.Cited in Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 proteinbioRxiv - Neuroscience 2017, published in eLife doi: 10.7554/eLife.24779Quote: ... Immunoprecipitated myc-PER2 were incubated with GST-tagged recombinant human SIK3 (aa.1-307) (SignalChem) in kinase assay buffer (50 mM Tris-HCl ... -
Dianova
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... About 5 µg of purified protein was used for immunoblot analysis using mouse α-His (1:2000) (Dianova, Germany) and Alexa488 antibodies. -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in eLife doi: 10.7554/eLife.52009Quote: Drosophila S2 cells were maintained in Insect-Xpress medium (Lonza) at 25°C ... -
Leica
No products found because this supplier's products are not listed.Cited in Biodistribution and inflammatory response to intracranial delivery of scintillating nanoparticlesbioRxiv - Bioengineering 2019Quote: ... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ... -
SouthernBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2017Quote: ... and purified recombinant antibodies were added to wells and detected with alkaline phosphatase-conjugated isotype-specific goat anti-mouse polyclonal serum (Southern Biotech). 2H1 antibodies were validated by indirect immunofluorescence with C ... -
Bio X Cell
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... 1000 U/mL recombinant IL-2 (eBioscience) was incubated with 50 μg/mL S4B6-1 (BioXcell) for 30 minutes at room temperature ...