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Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged
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Creative Biomart Sponsored
Recombinant Mouse Fzd1 extracellular domain (Met 1-His 248) (NP_067432.2), fused with a...Cat# Fzd1-2292M, 50.0 µg, USD $429.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular Biology of the Cell doi: 10.1091/mbc.E19-07-0362Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... 1 µg recombinant His-tagged mouse IgSF8 (Sino Biological) was incubated in 1 ml binding buffer (10 mM HEPES pH 7.4 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.02328Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ... -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: 6×His-tagged VHH-mCherry (Addgene #109421) was transformed into BL21DE3 E.coli cells ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and 1 U/ml recombinant mouse IFNγ (Peprotech) at 33°C ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2021Quote: ... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in Biochemical analysis of deacetylase activity of rice sirtuin OsSRT1, a class IV member in plantsbioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01905-18Quote: ... His-tagged proteins were detected with mouse anti-His tag (27E8) antibody at a dilution of 1:1000 (Cell Signaling, Danvers, MA) and an anti-mouse IgG HRP linked antibody at a dilution of 1:2000 (Cell Signaling ... -
Origene Technologies
No products found because this supplier's products are not listed.Cited in Destabilization of β-cell FIT2 by saturated fatty acids contribute to ER stress and diabetesbioRxiv - Biochemistry 2021Quote: ... transiently co-transfected with pcDNA3.1-FIT2/V5-His and GFP-tagged MARCH6 (BC059190) Mouse Tagged ORF Clone (Origene), were pre-treated with 10 µM MG132 (Cell Signaling Technology ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... His-tagged proteins were detected by a mouse anti-His antibody (sc-8036, 1:3000, Santa Cruz Biotechnology). GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138 ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... and sonic hedgehog homolog (Shh; 1:500, 20697-1-AP; Proteintech) overnight at 4°C ... -
Acrobiosystems
No products found because this supplier's products are not listed.Cited in SARS-CoV-2 Beta variant infection elicits potent lineage-specific and cross-reactive antibodiesbioRxiv - Immunology 2021Quote: His-tagged recombinant RBD Beta protein was produced in HEK cells (ACROBiosystems, SPD-C52Hp) and covalently labeled using CruzFluor488 (Santa Cruz Biotechnology ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
Active Motif
No products found because this supplier's products are not listed.Cited in G9a/GLP methyltransferases inhibit autophagy by methylation-mediated ATG12 protein degradationbioRxiv - Molecular Biology 2021Quote: Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ... -
Promega
No products found because this supplier's products are not listed.Cited in Orchestration of Chlorophyll and Carotenoid Biosynthesis by ORANGE Family Proteins in PlantbioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ... -
RayBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
Abnova
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... recombinant protein of the human homologs of GLAST and GLT-1 (Slc1a3, Cat# H00006507-P02; Slc1a2, Cat# H00006506-P01; Abnova, Taipei, Taiwan) were serially diluted in phosphate buffered saline (PBS ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-020-0380-1Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019Quote: ... Soluble mouse recombinant IL10 and IFNγ were procured from BD Biosciences (San Diego ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2017, published in Neuropsychopharmacology doi: 10.1038/s41386-018-0088-7Quote: ... followed by Cy3-tagged donkey antimouse antibody (1:500; Jackson ImmunoResearch). EYFP positive cells were identified using chicken anti-EGFP antibody (1:1000 ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Genetics 2021Quote: ... and (d) FITC tagged anti-mouse antibody (1:200 dilution; Vector Labs). After each incubation ... -
Genetex
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Journal of Thrombosis and Haemostasis doi: 10.1111/jth.15095Quote: ... 6 ng/mL recombinant mouse IL3 and 10 ng/mL recombinant human IL-6 (all from Stem Cell Technologies). On Day 0 ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... Recombinant Mouse Transglutaminase 2 was from Novus Biologicals, USA ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: Male Drosophila Schneider-2 (S2) cells were maintained in Schneider’s Drosophila Medium (Lonza) supplemented with 10% FCS and 1% penicillin-streptomycin at 28°C. -
BPS Bioscience
No products found because this supplier's products are not listed.Cited in Suramin potently inhibits binding of the mammalian high mobility group protein AT-hook 2 to DNAbioRxiv - Biochemistry 2019Quote: ... His-tagged BRD4-BD1 was purchased from BPS Bioscience (#31042). Pre-acetylated Biotin-Histone 4 Peptide was purchased from AnaSpec (#64989-025) ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ... -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ... -
LI-COR
No products found because this supplier's products are not listed.Cited in PARP7 mono-ADP-ribosylates the Agonist Conformation of the Androgen Receptor in the NucleusbioRxiv - Biochemistry 2021Quote: ... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... 1 μl of each primer tagged with Nextera XT adapter (Illumina, San Diego, USA) and 1ul of DNA template ... -
Abclonal Science
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... mouse anti-His (ABclonal AE003). -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ... -
Genesee Scientific
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: Drosophila species were cohabitated during dialect training in standard Drosophila bottles (Genesee catalog number 32-130) containing 50 mL standard Drosophila media ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Genetics 2018, published in PLOS Genetics doi: 10.1371/journal.pgen.1007658Quote: Mice carrying the murine homolog of the FVL mutation [29] (F5L; also available from Jackson Laboratories stock #004080) or the TFPI Kunitz domain deletion (Tfpi- ... -
Synaptic Systems
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse Gephyrin (1:1000, Synaptic Systems, catalog #147021), Neuroligin-1 (1:300 ...