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Recombinant Mouse CD8a Antigen Alpha Chain His tagged
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Creative Biomart Sponsored
Recombinant Mouse Cd8a (NP_001074579.1) extracellular domain (Met 1-Tyr 196), fused with a...Cat# Cd8a-2283M, 20.0 µg, USD $399.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2019, published in Molecular Biology of the Cell doi: 10.1091/mbc.E19-07-0362Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... 1 µg recombinant His-tagged mouse IgSF8 (Sino Biological) was incubated in 1 ml binding buffer (10 mM HEPES pH 7.4 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.02328Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.Cited in Pathobionts from chemically disrupted gut microbiota induce insulin-dependent diabetes in micebioRxiv - Microbiology 2022Quote: ... Anti-mouse-CD8a (551162, BD Pharmingen), Anti-mouse-F4/80 (12-4801-82 ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ... -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml. -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Alpha Diagnostic
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Goat Anti-Monkey IgG (alpha-chain specific)-HRP conjugate (1:5,000 dilutions, Alpha Diagnostic) and were used ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in mAbs doi: 10.1080/19420862.2020.1714371Quote: The recombinant biotinylated A33-His antigen was immobilized on streptavidin (StreptaWells, Roche) or avidin coated wells (Avidin ... -
New England Biolabs
No products found because this supplier's products are not listed.Cited in Biochemical analysis of deacetylase activity of rice sirtuin OsSRT1, a class IV member in plantsbioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ... -
Origene Technologies
No products found because this supplier's products are not listed.Cited in Destabilization of β-cell FIT2 by saturated fatty acids contribute to ER stress and diabetesbioRxiv - Biochemistry 2021Quote: ... transiently co-transfected with pcDNA3.1-FIT2/V5-His and GFP-tagged MARCH6 (BC059190) Mouse Tagged ORF Clone (Origene), were pre-treated with 10 µM MG132 (Cell Signaling Technology ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2022Quote: ... mouse CD8a (Cell Signaling Technology, 98941), and human CD8a (Biolegend ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... and anti-mouse CD8a/APC (clone 53-6.7, Miltenyi Biotec). -
Acrobiosystems
No products found because this supplier's products are not listed.Cited in SARS-CoV-2 Beta variant infection elicits potent lineage-specific and cross-reactive antibodiesbioRxiv - Immunology 2021Quote: His-tagged recombinant RBD Beta protein was produced in HEK cells (ACROBiosystems, SPD-C52Hp) and covalently labeled using CruzFluor488 (Santa Cruz Biotechnology ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... His-tagged proteins were detected by a mouse anti-His antibody (sc-8036, 1:3000, Santa Cruz Biotechnology). GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138 ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... before low pH antigen retrieval and staining with CD8a and Ki67 (Supplementary Table 7) antibodies and secondary antibody (EnVision+ HRP-rabbit, Dako, catalog #K400311-2) using the EnVision DuoFlex system (Dako) ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ... -
The Native Antigen Company
bioRxiv - Microbiology 2021Quote: ... and recombinant SARS-CoV S1 subunits-His tag (The Native Antigen Company, UK) diluted in phosphate-buffered saline (PBS ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and Goat anti-human IgA (alpha chain specific) (1/20000) (all from Jackson Immunoresearch). Secondary antibodies were incubated for 30 minutes at room temperature ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
Promega
No products found because this supplier's products are not listed.Cited in Orchestration of Chlorophyll and Carotenoid Biosynthesis by ORANGE Family Proteins in PlantbioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ... -
Proteintech
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... alpha-crystallin B chain (CRYAB, ProteinTech #15808-1-AP, 1:500), peripheral myelin protein 2 (PMP2 ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... plates were pre-incubated with either 25 nM or 50 nM recombinant ricin B chain (Vector Laboratories, USA) for 2 h before addition of 50 nM of rLA3490 or t3490 and a final 2 h incubation ... -
RayBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... recombinant human His-tagged ACE-2 protein (RayBiotech) was incubated on cells before adding any antibodies ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
Novus Biologicals
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Novus biological DM1A), Rabbit anti-calnexin (abcam 22595) ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ... -
SouthernBiotech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... and incubated with chicken IgY anti-DMPO (20μg/mL) and rat IgY anti-mouse CD8a (1:250, SouthernBiotech). DMPO adducts were detected with Alexa Fluor 488–conjugated goat anti-chicken IgY secondary antibody (1:400 ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-020-0380-1Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... TNF alpha (0.5ng/mL final concentration in PBS, human recombinant, Stemcell Tech) was added to half of the wells ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.Cited in The Ndc80-Cdt1-Ska1 complex constitute a minimal processive kinetochore-microtubule coupling unitbioRxiv - Cell Biology 2022Quote: ... HRP-tagged mouse/rabbit antibodies (Jackson laboratories) were used at 1:10,000 dilution to detect the bait-prey complex and blot was developed by Super signal west pico chemiluminescent substrate (Thermofisher). -
PBL Assay Science
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... Interferon alpha was measured by ELISA (Mouse IFN Alpha All Subtype ELISA Kit, High Sensitivity, PBL Assay Science). Blood was left at room temperature for 20 min and after centrifugation serum was frozen at -20°C until further analysis ... -
Bethyl
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG h+l HRP-tagged antibody (Bethyl Laboratories) diluted 1:20000 ... -
MyBioSource
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... Recombinant mouse HEXB protein (His Tag) was obtained from MyBioSource (San Diego, CA). Recombinant proteins alcohol dehydrogenase (ADH ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2022Quote: ... and CD8 alpha antigen retrieval was performed with BOND Epitope Retrieval 2 – ER2 (AR9640, Leica) for 40 ... -
Bio X Cell
No products found because this supplier's products are not listed.Cited in Dual blockade of IL-6 and CTLA-4 regresses pancreatic tumors in a CD4+ T cell-dependent mannerbioRxiv - Cancer Biology 2020Quote: ... anti-mouse CD8a (Clone 2.43) and anti-mouse CD4 (Clone GK1.5) depleting antibodies were purchased from BioXcell. -
Rockland
No products found because this supplier's products are not listed.bioRxiv - Genomics 2022Quote: ... and goat anti-human IgA (alpha chain) DyLight800 (Rockland Immunochemicals) antibodies at a final concentration of 0.1 μg/ml and 1 μg/ml ... -
Active Motif
No products found because this supplier's products are not listed.Cited in G9a/GLP methyltransferases inhibit autophagy by methylation-mediated ATG12 protein degradationbioRxiv - Molecular Biology 2021Quote: Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: Ten recombinant CD antigens were diluted to 0.5 mg/mL in Phosphate Buffered Saline (Corning Cellgro), or concentrated up to 0.5 mg/mL using 10K MWCO Amicon Ultra-15 centrifugal filters (Millipore) ...