-
No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
-
No products found
because this supplier's products are not listed.
Bhaskar Saha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... All GST-tagged proteins were expressed in Escherichia coli SoluBL21 (Genlantis). GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01) ...
-
No products found
because this supplier's products are not listed.
Rebekah B. Stuart, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant proteins were produced in Escherichia coli BL21 (DE3) cells (Bioline) as described previously (LaCourse ...
-
No products found
because this supplier's products are not listed.
Marisa Oliveira, et al.,
bioRxiv - Immunology 2020
Quote:
... and chicken interferon alpha (Yeast-derived Recombinant Protein, Kingfisher Biotech, Inc) were diluted in nuclease-free water (Ambion ...
-
No products found
because this supplier's products are not listed.
Pehuén Pereyra Gerber, et al.,
bioRxiv - Microbiology 2021
Quote:
... incubated for 30 min with a PE-labeled anti–human IgG-Fc antibody (Leinco/Biotrend), washed again ...
-
No products found
because this supplier's products are not listed.
Luisa Diomede, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... FLAG-tagged ACE2 (AdipoGen) was captured on the chip by a previously immobilized anti-FLAG antibody (Merck Life Science S.r.l) ...
-
No products found
because this supplier's products are not listed.
Matthew R. McFarland, et al.,
bioRxiv - Systems Biology 2020
Quote:
... HA-tagged Gln4 protein was detected using an anti-HA antibody (HA.11 clone 16B12, Cambridge Biosciences) and SuperSignal West Femto substrate (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Charles A Berdan, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
-
No products found
because this supplier's products are not listed.
Alex Hunt, et al.,
bioRxiv - Microbiology 2019
Quote:
... The shortCAP recombinant protein was used to immunize female New Zealand white rabbits (Covalab, Cambridge, United Kingdom) for generation of polyclonal antibodies.
-
No products found
because this supplier's products are not listed.
Anne-Cathrine Vogt, et al.,
bioRxiv - Immunology 2022
Quote:
Recombinant SARS-CoV-2 spike S1 B.1.1.529-Omicron RBD protein was purchased from (antibodies-online GmbH), recombinant SARS-CoV-2 Spike RBD and recombinant SARS-CoV-2 Spike RBD B.1.617.2 L452R T478K were purchased from R&D systems ...
-
No products found
because this supplier's products are not listed.
Paul A. Rowley, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
Human brain and testes tissue total protein lysates were purchased from ProSci Incorporated (catalogue numbers 1303 and 1313 ...
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Inc., Cat# CKH113).
-
No products found
because this supplier's products are not listed.
Andrea Di Francesco, et al.,
bioRxiv - Genetics 2023
Quote:
... Antibodies including Fc block (clone 2.42, Leinco Technologies) were added and incubated for 30 minutes at 4°C ...
-
No products found
because this supplier's products are not listed.
Firyal Ramzan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkyne-tagged fatty acid analogs (alkyne-myristate (13-tetradecylnoic acid; 13-TDYA; Click Chemistry Tools 1164) or alkyne-palmitate (15-hexadecynoic acid ...
-
No products found
because this supplier's products are not listed.
Ok-Hee Kim, et al.,
bioRxiv - Physiology 2019
Quote:
... and human intact FGF23 (Elabscience) were measured using enzyme-linked immunosorbent assay ...
-
No products found
because this supplier's products are not listed.
Jack A. Bryant, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
-
No products found
because this supplier's products are not listed.
Nicholas B. Karabin, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Pooled human plasma was acquired from Zen-Bio Inc ...
-
No products found
because this supplier's products are not listed.
Lars Gruber, et al.,
bioRxiv - Biochemistry 2023
Quote:
Monoculture and biculture spheroids of CCD-1137Sk human fibroblasts and HT-29 human colon cancer cells (both LGC Standards, Wesel, Germany) were prepared ...
-
No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
-
No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
-
No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
-
No products found
because this supplier's products are not listed.
Lien D. Nguyen, et al.,
bioRxiv - Neuroscience 2022
Quote:
Total protein was extracted using RIPA buffer (Boston Bioproducts) supplemented with Complete ...
-
No products found
because this supplier's products are not listed.
Emma T Crooks, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by alkaline phosphatase labeled anti-human Fc conjugate (Accurate Chemicals) and were developed using SigmaFast BCIP/NBT (Sigma).
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
WNT surrogate-Fc Fusion Protein (Wnt-surrogate; ImmunoPrecise Antibodies Ltd. ...
-
No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Reuben A. Saunders, et al.,
bioRxiv - Biophysics 2020
Quote:
... our ssrA-tagged titin protein was reacted at room temperature with three equivalents of ATTO 575Q maleimide (ATTO-TEC; GMBH) for 30 min in buffer A supplemented with 5 mM Tris (pH 8.0) ...
-
No products found
because this supplier's products are not listed.
Clàudia Salat-Canela, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
-
No products found
because this supplier's products are not listed.
Su Z. Hong, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Viral injection of the recombinant adeno-associated virus expressing Cre recombinase under the control of human synapsin promoter (rAAV-hSyn-Cre; from SignaGen Laboratories, Rockville, MD) was done by bulk regional viral injection to the visual cortical area of neonatal Cre dependent Gs-DREADD mice at p1 ...
-
No products found
because this supplier's products are not listed.
Dorothea Höpfner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... samples containing 100 ng recombinant protein were run over a 5 μm Jupiter C4 300Å LC column (Phenomenex, Torrance, California) using the 1260 Infinity LC system (Agilent Technologies ...
-
No products found
because this supplier's products are not listed.
Oktawia Nilsson, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and cholesterol (FC) (Avanti Polar Lipids) were dissolved in 3:1 chloroform:methanol ...
-
No products found
because this supplier's products are not listed.
Mariano Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
The full-length PfS1 recombinant purified protein was used to immunized rabbits subcutaneously following a two-months standard procedure (Proteogenix, France). Briefly ...
-
No products found
because this supplier's products are not listed.
Jan-Renier A.J. Moonen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Flag-tagged KLF4 (#VH829440, Vigene Biosciences) or GFP control (AVP004, GenTarget Inc) for 12 h after which cells were allowed to recover for 90 h before being harvested for subsequent experiments.
-
No products found
because this supplier's products are not listed.
Djem U. Kissiov, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... In all cases medium contained 5% FCS (Omega Scientific), 0.2 mg/mL glutamine (Sigma) ...
-
No products found
because this supplier's products are not listed.
Lindsay P. Winkenbach, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... from Stellaris LGC (Biosearch Technologies, BNS-5082 and FC-1065, respectively). Secondary ...
-
No products found
because this supplier's products are not listed.
Megha Rajendran, et al.,
bioRxiv - Biophysics 2022
Quote:
... FCS measurements were made in eight-well cover-glass slides (Grace Biolabs) pretreated with Sigmacoat (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron. In vitro transcribed single guide RNAs (sgRNAs ...
-
No products found
because this supplier's products are not listed.
Yueyuan Shi, et al.,
bioRxiv - Microbiology 2021
Quote:
... All cells were supplemented with 10% (v/v) fetal calf serum (FCS) (ABI) at 37 °C in 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Uri Elia, et al.,
bioRxiv - Immunology 2020
Quote:
... pseudouridine-substituted Fc-conjugated RBD mRNA (331-524 aa) was purchased from TriLink Bio Technologies (San Diego ...
-
No products found
because this supplier's products are not listed.
Emilie L. Cerezo, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Human EGF (Euromedex, Cat# HC88823), LJH685 (Selleck Chemicals ...
-
No products found
because this supplier's products are not listed.
Hazel Stewart, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Pacific Blue-conjugated anti-CD71 (Exbio, MEM-75, FC 1 μg / 100 μl / 106 cells), StrepMAB-Classic (IBA LifeSciences ...
-
No products found
because this supplier's products are not listed.
Jiongqin Liu, Xiao Han, Fei Tao, Ping Xu,
bioRxiv - Bioengineering 2023
Quote:
The recombinant plasmids were extracted using the TIANprep Rapid Mini Plasmid Kit (TIANGEN, Beijing, China). Genomic DNAs of bacteria were extracted using the Wizard Genomic DNA Purification Kit (Promega ...
-
No products found
because this supplier's products are not listed.
Veronica Jové, et al.,
bioRxiv - Cancer Biology 2023
Quote:
The open reading frame DNA sequences of human USP18(AA16-372) (Uniprot Q9UMW8) and human pro-ISG15(AA1-165)C78S (Uniprot P05161) were synthesized by GENEWIZ. C78S was substituted to stabilize ISG15 for purification (Narasimhan ...
-
No products found
because this supplier's products are not listed.
Khairunnisa Mentari Semesta, et al.,
bioRxiv - Cell Biology 2020
Quote:
sgRNA transduced cells seeded on 6-well plates at ~60% confluency were transfected with 2 μg flag-tagged β2-AR for 48 hr using JetPrime Transfection reagent (Genesee, Cat #55134) following manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Nadine R King, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 2 mg/ml Human Serum Albumin (HSA; Irvine Scientific), 10 μg/ml insulin (Sigma) ...
-
No products found
because this supplier's products are not listed.
Qinghui Wang, et al.,
bioRxiv - Immunology 2022
Quote:
... Naïve CD3 human T cells were purchased from HemaCare (Lot #21068415). N/TERT-1 cells were a gift from the Rheinwald Lab (Dickson et al. ...
-
No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
-
No products found
because this supplier's products are not listed.
Aloysius Wong, et al.,
bioRxiv - Plant Biology 2019
Quote:
The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
-
No products found
because this supplier's products are not listed.
Yeongjoon Lee, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Claire S. Chung, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... protein samples were spotted on carbon-coated Formvar grid (Ted Pella). The samples were stained with nanoW/uranyl acetate before air drying ...