-
No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
-
No products found
because this supplier's products are not listed.
Robert Bucki, et al.,
bioRxiv - Biophysics 2022
Quote:
... Recombinant human protein arginine deiminase 4 (PAD4) was from Cayman Chemical, Inc.
-
No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
-
No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Ming Wen, et al.,
bioRxiv - Genomics 2022
Quote:
... The Hi-C library was amplified using PE primers (Illumina) with 10 PCR amplification cycles ...
-
No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
-
No products found
because this supplier's products are not listed.
Jason S Presnell, William E Browne,
bioRxiv - Developmental Biology 2021
Quote:
... Recombinant Cas9 protein (PNA Bio) and sgRNAs were injected at concentrations of 400 ng/µl of Cas9 protein and 100 ng/µl for each sgRNA ...
-
No products found
because this supplier's products are not listed.
Noémie Aurine, et al.,
bioRxiv - Cell Biology 2019
Quote:
... or universal IFN-type (a recombinant human IFN-alpha protein hybrid; Pbl Assay Science, 11200-2). Bat cells seeded in 12-well plates at 80% confluence were treated for 6 h at 37°C with either poly IC (0–1000 ng/mL ...
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No products found
because this supplier's products are not listed.
Simone Caielli, et al.,
bioRxiv - Immunology 2023
Quote:
... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Chongyang Li, et al.,
bioRxiv - Cancer Biology 2019
Quote:
The bacterial expression vectors pReceiver-B11 for His-NSMCE2 and His-PFDN2 recombinant protein expression were purchased from Genecopoeia, Inc (Rockville ...
-
No products found
because this supplier's products are not listed.
Yoshiaki Kamiyama, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Both MBP-tagged and GST-tagged recombinant proteins were further purified with a Nanosep® 30-kDa size-exclusion column (PALL, NY). In addition ...
-
No products found
because this supplier's products are not listed.
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
-
No products found
because this supplier's products are not listed.
Olga Mitrofanova, et al.,
bioRxiv - Bioengineering 2023
Quote:
... 10 nM gastrin, 50 ng/ml recombinant human EGF (R&D, 236-EG) and 500 nM A83-01 (Tocris). Organoids were passaged every 4 days by physical dissociation using fire-polished Pasteur pipettes.
-
No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
-
No products found
because this supplier's products are not listed.
Michelle E. Poling, et al.,
bioRxiv - Physiology 2022
Quote:
... Recombinant GFP protein (Cat# MB-0752, Vector labs), diluted in S2R+ cell growth media (64ng/μl to 4 ng/μl) ...
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No products found
because this supplier's products are not listed.
Alfred Kihoon Lee, et al.,
bioRxiv - Neuroscience 2022
Quote:
... r-peptide) and biotin-tagged Aβ (1–42) (Cat# AS-23523-05, 0.5 mg, Anaspec) were used to make oligomeric forms as described in our previous study (Naito et al. ...
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No products found
because this supplier's products are not listed.
Kevin W. Ng, et al.,
bioRxiv - Immunology 2020
Quote:
... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
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No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
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No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
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No products found
because this supplier's products are not listed.
Nicholas W. Chavkin, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Ror1/2fl/fl + Ubc-CreERT2 mice were generated by combining Ror1 flox (Jackson Labs #018353,40), Ror2 flox (Jackson Labs #018354,40) ...
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No products found
because this supplier's products are not listed.
Christian Hentrich, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and freshly reduced proteins were mixed with malemide-labeled PE (Expedeon) or HRP (Thermo-Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Melanie Werner-Klein, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... PE-labeled streptavidin (Dianova, Germany) was used as secondary staining reagent ...
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No products found
because this supplier's products are not listed.
Timothy S. Strutzenberg, et al.,
bioRxiv - Biophysics 2019
Quote:
... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
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No products found
because this supplier's products are not listed.
Heather M. Forsythe, et al.,
bioRxiv - Biophysics 2019
Quote:
... The plasmid containing the gene for human γS WT was engineered into the pE-SUMO (Small Ubiquitin-like Modifier) vector containing a N-terminal 6XHis tagged fusion protein (LifeSensors Inc., Malvern, PA). The γS N14D and γS N76D were generated via site-directed mutagenesis using QuikChangeXL Kit (Agilent Technologies ...
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No products found
because this supplier's products are not listed.
Francois Chesnais, et al.,
bioRxiv - Bioengineering 2022
Quote:
... a co-culture of 7.5×103 Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) and 1×105 Normal Human Dermal Fibroblasts (NHDF, Promocell) or Human Mesenchymal Stem cells (MSC ...
-
No products found
because this supplier's products are not listed.
Tommy Stormberg, Yuri L. Lyubchenko,
bioRxiv - Molecular Biology 2022
Quote:
... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
-
No products found
because this supplier's products are not listed.
Min Zhang, et al.,
bioRxiv - Biophysics 2022
Quote:
... Alexa Fluor 647 labeled HI was excited by 640nm solid state laser lines (Olympus). We found the optimal ratio of labeled to unlabeled insulin for REPLOM to be ca ...
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No products found
because this supplier's products are not listed.
Rebekah B. Stuart, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant proteins were produced in Escherichia coli BL21 (DE3) cells (Bioline) as described previously (LaCourse ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
No products found
because this supplier's products are not listed.
James Kaminski, et al.,
bioRxiv - Immunology 2023
Quote:
... and recombinant human IL-2 (rhIL2,50 U/mL; R&D Systems/CellGenix)) with or without 1% penicillin/streptomycin/l-glutamine (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
David Cluet, et al.,
bioRxiv - Biochemistry 2019
Quote:
... HA tagged proteins were labeled overnight at 4°C with the mouse HA.11 Clone 16B12 Monoclonal Antibody (Eurogentec) 1/2000 in PBS + tween 0.2% (v/v ...
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No products found
because this supplier's products are not listed.
Advika Kamatar, et al.,
bioRxiv - Biophysics 2023
Quote:
... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
-
No products found
because this supplier's products are not listed.
Sabal Chaulagain, et al.,
bioRxiv - Microbiology 2020
Quote:
... and imaged with FluorChem R (Protein Simple). Densitometric analysis was performed using ImageJ software and all readings were normalized to GAPDH values.
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No products found
because this supplier's products are not listed.
Jenna Kropp Schmidt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... was conjugated to Alexa 647 using the Biotium Mix-n-Stain R-PE(PE) Antibody Labeling kit (Biotium, cat no: 92298) following the manufacturers protocol ...
-
No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
No products found
because this supplier's products are not listed.
Pablo Martinez, et al.,
bioRxiv - Plant Biology 2019
Quote:
Taxol stabilized rhodamine labeled microtubules and HIS-TAN1-GFP-Atto488 were visualized on an inverted Nikon Ti stand (Nikon) with a W1 spinning disk (Yokogawa ...
-
No products found
because this supplier's products are not listed.
Hye-Young Lee, et al.,
bioRxiv - Plant Biology 2020
Quote:
Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
-
No products found
because this supplier's products are not listed.
Firdousi Parvez, et al.,
bioRxiv - Biophysics 2023
Quote:
... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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No products found
because this supplier's products are not listed.
Melanie Ernst, et al.,
bioRxiv - Biophysics 2023
Quote:
Lissamine RhodamineB (RhB, acceptor) and 7-nitro-2-1,3-benzoxadiazol-4-yl-(NBD, donor) labeled PE lipids (Avanti Polar Lipids #810150 & #810145) were spiked in while preparing EPL liposomes at 1:40 for NBD-PE and 1:8 for RhB-PE ...
-
No products found
because this supplier's products are not listed.
Inga V. Hochheiser, et al.,
bioRxiv - Immunology 2021
Quote:
The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
-
No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...