-
No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Federico Bertoglio, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Fc-tagged ligand versions were labelled indirectly by using goat-anti-mFc-APC (Dianova) or mouse anti-hFcγ-APC (Biolegend ...
-
No products found
because this supplier's products are not listed.
Bastijn J.G. van den Boom, et al.,
bioRxiv - Neuroscience 2022
Quote:
... DBS parameters were programmed in a digital stimulator (DS8000, WPI, USA) and generated by isolators (DLS100 ...
-
No products found
because this supplier's products are not listed.
Wenxiang Xue, et al.,
bioRxiv - Microbiology 2024
Quote:
... Recombinant Human TNF-α (P00029) was purchased from Solarbio, China ...
-
No products found
because this supplier's products are not listed.
Timothy S. Strutzenberg, et al.,
bioRxiv - Biophysics 2019
Quote:
... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
No products found
because this supplier's products are not listed.
Ramakanth Neeli-Venkata, et al.,
bioRxiv - Cell Biology 2020
Quote:
Cell death assays were performed on a wide-field fluorescence microscope (TI-Eclipse; Nikon) operated with Micro-Manager (Open Imaging) ...
-
No products found
because this supplier's products are not listed.
Hritik Chandore, et al.,
bioRxiv - Cell Biology 2020
Quote:
... MCCF-7 cancer cells were examined for viability and cell death using a fluorescent microscope (OLYMPUS, Japan) (Kumar et al. ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Rufo Nicole, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... cells were treated with cell death inducers in presence or absence of 50 μM z-Vad-FMK (Bachem) in medium containing 1 μM sytox green (Thermofisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Augustus J. Lowry, et al.,
bioRxiv - Biophysics 2024
Quote:
... the cells were incubated in AnV buffer (1:175 dilution of the fluorescently tagged AnV (Biotium) in Hank’s balanced salt solution ...
-
No products found
because this supplier's products are not listed.
Le Zhang, et al.,
bioRxiv - Microbiology 2024
Quote:
... It was programmed to measure the OD hourly at 595 nm (Gen5 software, BioTek Instruments Inc., USA). The minimum inhibitory concentration was determined as the concentration of antibiotic where no visible growth was observed.
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No products found
because this supplier's products are not listed.
Maria Steene Eriksen, et al.,
bioRxiv - Biochemistry 2019
Quote:
Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
-
No products found
because this supplier's products are not listed.
Pasquale Valenzisi, et al.,
bioRxiv - Cell Biology 2024
Quote:
... recombinant anti-S9.6 (rabbit-monoclonal, Kerafast; 1:200), anti-RAD51 (rabbit-polyclonal ...
-
Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in...
Cat# LK003265,
5 ea, $50.00
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Meiling Zhang, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Recombinant HIV (Worthington) and HiScript III (Vazyme ...
-
No products found
because this supplier's products are not listed.
Dinesh Devadoss, et al.,
bioRxiv - Physiology 2020
Quote:
Normal Human Bronchial Epithelial Cells (NHBE) cells were obtained from MatTek Incorp (EpiAirway™ ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Maxime Lecerf, et al.,
bioRxiv - Immunology 2020
Quote:
... Biotinylated recombinant human FcRn (Amsbio LLC, Cambridge, MA) was diluted to 5 μg/ml in running buffer - 100 mM Tris pH 6 (adjusted by addition of dry citric acid) ...
-
No products found
because this supplier's products are not listed.
Lamar S. Thomas, Laura C. Cook,
bioRxiv - Microbiology 2022
Quote:
... The Tecan HP Infinite 200 Pro spectrophotometer was programmed by Tecan i-control software for incubation at 37°C and measurement of OD600 every 30 minutes with shaking for 10 secs prior to measurement ...
-
No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Caroline Atyeo, et al.,
bioRxiv - Immunology 2021
Quote:
... Avi-Tagged FcRs (Duke Human Vaccine Institute) were biotinylated using BirA500 kit (Avidity) per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Georgia Balsevich, et al.,
bioRxiv - Neuroscience 2022
Quote:
... recombinant mouse leptin (Cedarlane Laboratories Ltd, CLCYT351-2), and Compound C (Selleck Chemicals ...
-
No products found
because this supplier's products are not listed.
Nawar Naseer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products) for 6 days to promote differentiation into hMDMs ...
-
No products found
because this supplier's products are not listed.
Srividhya Swaminathan, et al.,
bioRxiv - Immunology 2021
Quote:
... the cells were washed and cultured in 48-well plates for 2 weeks supplemented with recombinant interleukin-2 (IL-2, Charles River Labs, USA) at 20 IU / mL every 2–3 days thereafter ...
-
No products found
because this supplier's products are not listed.
Alina Ainbinder, et al.,
bioRxiv - Genetics 2021
Quote:
... cells were washed once with HI media (BioIVT) and siRNAs in HI media were added at a final concentration of 1 μM ...
-
No products found
because this supplier's products are not listed.
Radhika Gudi, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Unconjugated EGF ligand was purchased from Tonbo biosciences, Alexa fluor-555 conjugated EGF (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Waipan Chan, et al.,
bioRxiv - Genomics 2020
Quote:
BMDM or RAW cells were stimulated with TLR4 ligand Kdo2-Lipid A (KLA, Avanti Polar Lipids). Ligand addition was staggered so that for all time points ...
-
No products found
because this supplier's products are not listed.
Ryan J. Garrigues, et al.,
bioRxiv - Biochemistry 2022
Quote:
... and 2% normal human serum (NHS; Innovative Research) in GHB++ (10 mM HEPES (pH 7.3) ...
-
No products found
because this supplier's products are not listed.
Liangbo Qi, et al.,
bioRxiv - Biophysics 2019
Quote:
... 3.5 μl aliquots of the recombinant human TIM22 complex (7 mg ml-1) were dropped onto glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Yinghui Li, et al.,
bioRxiv - Pathology 2022
Quote:
... and cell death (blue coloration) were examined in the leaf tissues under a stereo microscope Zeiss Axio Imager M2 (Carl Zeiss, Germany). All microscopic experiments were repeated thrice ...
-
No products found
because this supplier's products are not listed.
Amit Bachar, et al.,
bioRxiv - Microbiology 2019
Quote:
... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... beads were incubated with varying amount of the recombinant his-tagged proteins and corresponding MFIs were compared to reference beads of know AlexFluor molecule densities (Bangs Laboratories, IN).
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
-
No products found
because this supplier's products are not listed.
Anne-Marinette Cao, et al.,
bioRxiv - Biophysics 2021
Quote:
The pcDNA plasmid encoding SNAP-tagged human mGluR2 was a gift from Cisbio Bioassays (Codolet ...
-
No products found
because this supplier's products are not listed.
Gabriel Ozorowski, et al.,
bioRxiv - Biophysics 2020
Quote:
The ChemBridge ligand library (1.3M compounds [https://www.chembridge.com/] (accessed February 2019)] ...
-
No products found
because this supplier's products are not listed.
Shutang Tan, et al.,
bioRxiv - Plant Biology 2019
Quote:
... membranes were incubated for 2 h at room temperature with an anti-His HRP-(horseradish peroxidase) conjugated antibody (1:4000; Agrisera) or an anti-GST HRP-conjugated antibody (1:4000 ...
-
No products found
because this supplier's products are not listed.
Stefania Di Blasio, et al.,
bioRxiv - Immunology 2019
Quote:
... supplemented with 2% human serum (HS, Sanquin). Autologous or allogenic CD3+ T cells used in T cell co-culture experiments were isolated using the Pan T cell isolation kit (Miltenyi Biotec ...
-
Denosumab (anti-RANK ligand) (AMG-162, Prolia, Xgeva) is a fully human monoclonal antibody that...
Cat# A2020, SKU# A2020-1mg,
1mg, $239.00
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Jonuelle Acosta, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Drugs or cell death inhibitors are used at the following concentrations: Necrostatin-1s (10μg/mL, Selleck Chemicals), Ferrostatin-1 (2μM ...
-
No products found
because this supplier's products are not listed.
Rasmus Riemer Jakobsen, et al.,
bioRxiv - Microbiology 2021
Quote:
The human colon adenocarcinoma cell line Caco-2 (ATTC HTB-37, LGC standards, Middlesex, UK) at passage 53 was grown in Dulbecco’s Modified Eagles Medium (DMEM ...
-
No products found
because this supplier's products are not listed.
Luisa Diomede, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... FLAG-tagged ACE2 (AdipoGen) was captured on the chip by a previously immobilized anti-FLAG antibody (Merck Life Science S.r.l) ...
-
No products found
because this supplier's products are not listed.
Martin H. Berryer, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Starting on day 2 human induced neural progenitor-like cells were harvested with Accutase (Innovative Cell Technology, Inc., AT104-500) and re-plated at 15,000 cells.cm−2 in Astrocyte Medium (ScienCell ...
-
12 well plate with tissue culture treated #1.5 glass-like polymer cover slip (0.175±0.010mm)....
Cat# P12-1.5P,
20/case, $221.00
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Minghui Di, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HeLa cells were transfected with DNA (1 ug) expressing mCherry-tagged proteins cultured on 29-mm glass-bottom dish (Cellvis); plasmids (1 ug ...
-
No products found
because this supplier's products are not listed.
Jan Fischer, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
-
No products found
because this supplier's products are not listed.
Bettina P Mihalas, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated in human polyclonal anti-centromere protein antibody (1:50; Antibodies Incorporated, 15-235), rabbit polyclonal anti-SGOL2 antibody (1:200 ...
-
No products found
because this supplier's products are not listed.
Chop Yan Lee, et al.,
bioRxiv - Systems Biology 2023
Quote:
... For each passage 1-2×106 cells were seeded in T25 flasks (Sarstedt). Then ...
-
No products found
because this supplier's products are not listed.
Manuel Albanese, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human primary B cells were prepared from adenoidal mononuclear cells by Ficoll Hypaque (PAN Biotech) gradient centrifugation (as described in Albanese et al. ...