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No products found
because this supplier's products are not listed.
Samuele Cancellieri, et al.,
bioRxiv - Genetics 2021
Quote:
... and 100 ng ml-1 recombinant human FMS-like Tyrosine Kinase 3 Ligand (Flt3-L) (CellGenix cat# 1415-050). HSPCs were electroporated with 3xNLS-SpCas9:sg1617 RNP or HiFi-3xNLS-SpCas9:sg1617 RNP 24 h after thawing ...
-
No products found
because this supplier's products are not listed.
Changlin Du, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
-
Recombinant Human CD28 (P10747-1) extracellular domain (Met 1-Pro 152), fused with a...
Cat# CD28-3910H,
50ug , USD $298
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Jun Weng, et al.,
bioRxiv - Biochemistry 2021
Quote:
Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
-
No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Ida Lindeman, et al.,
bioRxiv - Immunology 2023
Quote:
... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
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No products found
because this supplier's products are not listed.
Oskar Staufer, et al.,
bioRxiv - Immunology 2022
Quote:
... cells were stained with anti-human ICAM-1-AlexaFluor647 (BioXcell, BE0020-2, 3 µM), anti-human CD58-AlexaFluor647 (BD Bioscience ...
-
No products found
because this supplier's products are not listed.
Esther A. Mozipo, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and human recombinant bFGF-2 (10ng/mL; ABM). DMEM-F12 w/o L-Glutamine w/o Hepes w/o Glucose (Biowest ...
-
No products found
because this supplier's products are not listed.
Jessica Gomez-Garrido, et al.,
bioRxiv - Genomics 2023
Quote:
... and Hi-C (Illumina NovaSeq 6000, 2×150bp) for chromosome-level scaffolding (Fig ...
-
No products found
because this supplier's products are not listed.
Nathan Jariwala, et al.,
bioRxiv - Cell Biology 2023
Quote:
... were cultured in HDF growth media supplemented with FBS (2% v/v) basic fibroblast growth factor (recombinant human; 1ng/ml) and insulin (5 μg/ml) (all from PromoCell). All cells were grown in a humidified environment at 37°C with 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
-
No products found
because this supplier's products are not listed.
Emily Clement, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... programmed using MetaMorph software (Molecular Devices, San José, CA, USA). All images were processed using the same filter to reduce noise with ImageJ software ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... 10 ng/ml recombinant human TNFα (Cell Applications, cat# RP1111-50) was added for 3 hours ...
-
No products found
because this supplier's products are not listed.
Amin Addetia, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
-
No products found
because this supplier's products are not listed.
Ilona Berestjuk, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Human lymphatic fibroblasts (FRC#1 and #2) were purchased from ScienCell Research Laboratories ...
-
No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
-
No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
His-tagged SSRP1 was PCR amplified from SSRP1 pReceiver (GeneCopoeia) using primers encoding an N-terminal His tag and NdeI/NotI cut sites for cloning into pET22b ...
-
No products found
because this supplier's products are not listed.
Sanchita Mishra, et al.,
bioRxiv - Immunology 2024
Quote:
... 100ng ml-1 recombinant human TNF-α(Abclonal, RP00001) was added.Tb1 Lu cells were treated with 30µM Z-VAD(OMe)-FMK and 1µM SM-164 2 hours before treatment with 100ng ml-1 TNF-α ...
-
No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
-
No products found
because this supplier's products are not listed.
Michael TH Ng, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The decloaking chamber is programmed to incubate slides in Citrate solution (Vector Labs, H3300) for 20 minutes reaching 110°C and 6.1 PSI ...
-
No products found
because this supplier's products are not listed.
Monica Varela, et al.,
bioRxiv - Immunology 2019
Quote:
... For cell death assays (Sytox Green uptake) cells were seeded in μ-Slide 8 well glass bottom (IBIDI) at a density of 1·105 cells/well and infections were performed for 6 hours ...
-
No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Efil Bayam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 2% glucose and the appropriate –His dropout mix (CSM-His, MP Biomedicals) or in autophagy induction medium SD-N ...
-
No products found
because this supplier's products are not listed.
Tommy Stormberg, Yuri L. Lyubchenko,
bioRxiv - Molecular Biology 2022
Quote:
... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
-
No products found
because this supplier's products are not listed.
Rie Asada Kitamura, et al.,
bioRxiv - Genetics 2021
Quote:
Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
-
No products found
because this supplier's products are not listed.
Darrell Pilling, et al.,
bioRxiv - Immunology 2022
Quote:
... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
No products found
because this supplier's products are not listed.
Máté Fischer, et al.,
bioRxiv - Bioengineering 2019
Quote:
... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
-
No products found
because this supplier's products are not listed.
Amanda L. Photenhauer, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
-
No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Álvaro Inglés-Prieto, et al.,
bioRxiv - Neuroscience 2020
Quote:
... cells were treated with recombinant human GDNF (Shenandoah Biotechnology) and human GFRα1 (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and 1 nM of recombinant human G6PD) in a dark 96-well plate (Greiner Bio-one). After 1 min incubation in the dark at 28◦C ...
-
Cat# HY-P7996,
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Hui Lin, et al.,
bioRxiv - Cancer Biology 2022
Quote:
Cell death was measured by staining with SYTOX Green (MedChemExpress, United States) to detect the plasma membrane integrity through FCM according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Jing-Ping Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... prepared from patient donors) or 200μg recombinant human myelin oligodendrocyte glycoprotein (hMOG 1–125, AS-55158-1000, AnaSpec) emulsified in complete (CFA ...
-
No products found
because this supplier's products are not listed.
Peng Dong, et al.,
bioRxiv - Genomics 2022
Quote:
... Mouse embryonic stem cells were plated on Human recombinant laminin 511-coated coverslips (Electron Microscopy Sciences, Cat. 72196-25). Cells were then fixed using 4% formaldehyde (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Mohammad M. Al-bataineh, et al.,
bioRxiv - Physiology 2022
Quote:
... Secondary HRP-tagged antibodies were from Jackson Labs (dilution 1:10,000). Blots were developed using Bio-Rad Clarity ECL reagent (3 min ...
-
No products found
because this supplier's products are not listed.
S. Jordan Kerns, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Human alveolar epithelial cells (Cell Biologics, Accegen) were cultured using SABM medium (Lonza ...
-
Cell Systems Cell Freezing Medium is a specialized medium. When used in conjunction with Cell...
Cat# 4Z0-705,
100.0 mL, $135.0
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Changsheng Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Human umbilical vein endothelial cells (HUVECs, Cell System) were cultured in endothelial cell growth medium according to the protocol provided by the manufacturer (VascuLife ...
-
No products found
because this supplier's products are not listed.
Florian Wernert, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Chicken anti-map2 (against residues 2-314 of human map2, 1:1000 for IF) was from Synaptic Systems (#188006). Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin ...
-
No products found
because this supplier's products are not listed.
Gautam Mahajan, et al.,
bioRxiv - Microbiology 2022
Quote:
Primary human vaginal epithelial cells (Lifeline Cell Technology, cat ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...