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No products found
because this supplier's products are not listed.
Changlin Du, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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No products found
because this supplier's products are not listed.
Jun Weng, et al.,
bioRxiv - Biochemistry 2021
Quote:
Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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No products found
because this supplier's products are not listed.
S. Marina Casalino-Matsuda, et al.,
bioRxiv - Immunology 2020
Quote:
Human monocytic leukemia THP-1 cells (American Type Culture Collection) were cultured in RPMI media and differentiated to a MØ phenotype by exposure to 5 nM PMA for 48 h (17).
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No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
His-tagged SSRP1 was PCR amplified from SSRP1 pReceiver (GeneCopoeia) using primers encoding an N-terminal His tag and NdeI/NotI cut sites for cloning into pET22b ...
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No products found
because this supplier's products are not listed.
James Mason, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... cells were treated with 1µM of recombinant human A83-01 (TOCRIS), or 20ng/ml recombinant TGF-β (R&D Systems ...
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No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... 10 ng/ml recombinant human TNFα (Cell Applications, cat# RP1111-50) was added for 3 hours ...
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No products found
because this supplier's products are not listed.
Francois Chesnais, et al.,
bioRxiv - Bioengineering 2022
Quote:
... a co-culture of 7.5×103 Red Fluorescent Protein-tagged HUVEC (RFP-HUVEC, 2Bscientific, UK) and 1×105 Normal Human Dermal Fibroblasts (NHDF, Promocell) or Human Mesenchymal Stem cells (MSC ...
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No products found
because this supplier's products are not listed.
Jun-Gu Kang, et al.,
bioRxiv - Microbiology 2019
Quote:
... His-tagged Gn and Gc recombinant proteins were purchased from Immune Technology Co ...
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No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
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No products found
because this supplier's products are not listed.
Emily Clement, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... programmed using MetaMorph software (Molecular Devices, San José, CA, USA). All images were processed using the same filter to reduce noise with ImageJ software ...
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No products found
because this supplier's products are not listed.
Tommy Stormberg, Yuri L. Lyubchenko,
bioRxiv - Molecular Biology 2022
Quote:
... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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No products found
because this supplier's products are not listed.
Xingxing Zhu, et al.,
bioRxiv - Immunology 2024
Quote:
... and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell) and 10 ng/mL recombinant mouse IL-4 ...
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No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
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No products found
because this supplier's products are not listed.
Quinton Smith, Christopher Chen, Sangeeta Bhatia,
bioRxiv - Bioengineering 2021
Quote:
Human intrahepatic biliary epithelial cells (IHCs) passage 1-5 (ScienCell, Carlsbad, CA) were cultured in complete epithelial growth media (ScienCell ...
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No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
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No products found
because this supplier's products are not listed.
Ida Lindeman, et al.,
bioRxiv - Immunology 2023
Quote:
... 3 µg/ml recombinant human TG2 or 2 µg/ml goat anti-human Ig (Southern Biotech) was coated in PBS followed by incubation with culture supernatants diluted in PBS supplemented with 0.1% (v/v ...
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No products found
because this supplier's products are not listed.
Michael TH Ng, et al.,
bioRxiv - Cell Biology 2023
Quote:
... The decloaking chamber is programmed to incubate slides in Citrate solution (Vector Labs, H3300) for 20 minutes reaching 110°C and 6.1 PSI ...
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No products found
because this supplier's products are not listed.
Joseph Atherton, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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No products found
because this supplier's products are not listed.
S. Jordan Kerns, et al.,
bioRxiv - Cancer Biology 2021
Quote:
Human alveolar epithelial cells (Cell Biologics, Accegen) were cultured using SABM medium (Lonza ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
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No products found
because this supplier's products are not listed.
Sanchita Mishra, et al.,
bioRxiv - Immunology 2024
Quote:
... 100ng ml-1 recombinant human TNF-α(Abclonal, RP00001) was added.Tb1 Lu cells were treated with 30µM Z-VAD(OMe)-FMK and 1µM SM-164 2 hours before treatment with 100ng ml-1 TNF-α ...
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No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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No products found
because this supplier's products are not listed.
Rie Asada Kitamura, et al.,
bioRxiv - Genetics 2021
Quote:
Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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No products found
because this supplier's products are not listed.
Pille Hallast, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries using 1.5 M human cells as input were generated with Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) following the manufacturer’s protocol with the following modification ...
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No products found
because this supplier's products are not listed.
Monica Varela, et al.,
bioRxiv - Immunology 2019
Quote:
... For cell death assays (Sytox Green uptake) cells were seeded in μ-Slide 8 well glass bottom (IBIDI) at a density of 1·105 cells/well and infections were performed for 6 hours ...
-
No products found
because this supplier's products are not listed.
Darrell Pilling, et al.,
bioRxiv - Immunology 2022
Quote:
... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Ana F. Ferreira, et al.,
bioRxiv - Neuroscience 2023
Quote:
Human samples: qPCR experiments were conducted using the SensiFAST Probe Hi-ROX Master Mix (Bioline) and the TaqMan Gene Expression Assays (Applied Biosystems ...
-
Cell Systems Cell Freezing Medium is a specialized medium. When used in conjunction with Cell...
Cat# 4Z0-705,
100.0 mL, $135.0
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Changsheng Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Human umbilical vein endothelial cells (HUVECs, Cell System) were cultured in endothelial cell growth medium according to the protocol provided by the manufacturer (VascuLife ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Yukiko Yamaguchi, et al.,
bioRxiv - Immunology 2022
Quote:
... containing 100 U/mL recombinant human IL-2 (rhIL-2, Novartis Oncology) and 0.5 ng/mL recombinant human IL-15 (rhIL-15, CellGenix). For CAR lentiviral transduction ...
-
No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
... and 1 nM of recombinant human G6PD) in a dark 96-well plate (Greiner Bio-one). After 1 min incubation in the dark at 28◦C ...
-
No products found
because this supplier's products are not listed.
Jing-Ping Lin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... prepared from patient donors) or 200μg recombinant human myelin oligodendrocyte glycoprotein (hMOG 1–125, AS-55158-1000, AnaSpec) emulsified in complete (CFA ...
-
No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Amanda L. Photenhauer, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Gautam Mahajan, et al.,
bioRxiv - Microbiology 2022
Quote:
Primary human vaginal epithelial cells (Lifeline Cell Technology, cat ...
-
No products found
because this supplier's products are not listed.
Carole Seguin-Devaux, et al.,
bioRxiv - Immunology 2024
Quote:
... or rabbit anti-His (Bethyl, ImTec Diagnostic NV ...
-
No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
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No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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No products found
because this supplier's products are not listed.
Michael J Abrams, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... and 0.1 mg/mL insulin (human recombinant, MP Biomedicals 0219390080). To introduce the molecular factors ...
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No products found
because this supplier's products are not listed.
Máté Fischer, et al.,
bioRxiv - Bioengineering 2019
Quote:
... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
-
No products found
because this supplier's products are not listed.
Awadalkareem Adam, et al.,
bioRxiv - Immunology 2021
Quote:
... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
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No products found
because this supplier's products are not listed.
Mohammad M. Al-bataineh, et al.,
bioRxiv - Physiology 2022
Quote:
... Secondary HRP-tagged antibodies were from Jackson Labs (dilution 1:10,000). Blots were developed using Bio-Rad Clarity ECL reagent (3 min ...