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No products found
because this supplier's products are not listed.
Xiaowei Sun, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Recombinant human insulin (M9194) was purchased from AbMole (Houston, USA). The ARF1 inhibitor Golgicide A (HY-100540 ...
-
No products found
because this supplier's products are not listed.
Jiao Wang, et al.,
bioRxiv - Immunology 2020
Quote:
... Recombinant human interleukin-12 (IL-2) was gifted from Akron Biotech. Recombinant human interleukin-15 (IL-15 ...
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
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No products found
because this supplier's products are not listed.
Nandan Haloi, et al.,
bioRxiv - Biophysics 2020
Quote:
... followed by addition of HKII (human recombinant HKII, 60 kU/ml; Genway Biotech, Slan Diego, CA) into the cis chamber ...
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No products found
because this supplier's products are not listed.
Sandro Roier, et al.,
bioRxiv - Immunology 2023
Quote:
... The protein vaccines were alum-adjuvanted by adsorption of the recombinant fusion proteins to aluminum hydroxide (Alhydrogel®; Croda, Frederikssund, Denmark) as previously described22 ...
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No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Nanami Sakata, et al.,
bioRxiv - Plant Biology 2022
Quote:
... L-His (Tokyo Chemical Industry), and L-Lys (Nacalai tesque ...
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No products found
because this supplier's products are not listed.
Kelly M. Hennessey, et al.,
bioRxiv - Cell Biology 2020
Quote:
... the C terminus of GL50803_8445 was tagged with an 11-amino acid flexible linker and the fluorescent protein mNeonGreen (Allele Biotechnology). The mNG-N11-Neo vector was constructed for this purpose by amplifying mNeonGreen using the primers listed in Table S1 ...
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No products found
because this supplier's products are not listed.
Md. Abu Sayeed, et al.,
bioRxiv - Immunology 2022
Quote:
The purified recombinant ICP1 bacteriophage proteins were used to raise mAbs via a commercial vendor (ProMab Biotechnologies, Inc.) using a conventional hybridoma technique (28) ...
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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Cat# PR-29bt,
500 micrograms,USD $420.0
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Yang Su, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Biotinylated M25 IgG1 and Streptavidin-ZAP (Advanced Targeting Systems) were mixed at a molar ratio of 1:1 on ice for 30 min and added to cells at final concentration of SA-ZAP at 0 ...
-
No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Recombinant mouse TNFSF11 (IBI Scientific, Indiana), also known as RANKL ...
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No products found
Ekaterina V. Tarasovetc, et al.,
bioRxiv - Cell Biology 2020
Quote:
... blocked with 2 mM biotinylated dPEG (2.5 kDa, Quanta BioDesign), washed extensively by centrifuging three times at 2,000 g ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... hPC antigen (hPC:Ag) was measured using the Human Protein C AssayMaxTM ELISA Kit (Assaypro, St. Charles, MO) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
David Steinbrecht, et al.,
bioRxiv - Systems Biology 2024
Quote:
Three independently passaged biological replicates of mESCs (∼3.5 × 107 cells per replicate) were cultured in “2i + LIF” ES medium supplemented with 1µM flavopiridol (HY-10005-10mg, Biotrend) to block the transcription ...
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No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
-
No products found
because this supplier's products are not listed.
Peter J. Bosch, et al.,
bioRxiv - Neuroscience 2023
Quote:
Images that were collected on the Leica Sp5 microscope were collected as .lif files and imported directly into Imaris Microscopy Image analysis software (Oxford Instruments). Filaments were traced along basilar dendrites beginning at least 20 µm from the soma by an experimenter blinded to treatment conditions ...
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No products found
because this supplier's products are not listed.
Ankit Rai, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Biotinylated poly(l-lysine)-[g]-poly(ethylene glycol) (PLL-PEG-biotin) was obtained from Susos AG ...
-
No products found
because this supplier's products are not listed.
Huan Ma, et al.,
bioRxiv - Immunology 2022
Quote:
... The recombinant vectors were transiently transfected into HEK293F cells with polyethyleneimine (Polyscience). After three days of expression ...
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No products found
because this supplier's products are not listed.
Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical ...
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No products found
because this supplier's products are not listed.
Xinyu Xie, et al.,
bioRxiv - Immunology 2024
Quote:
... media containing PBS or recombinant IL-1β (10 ng/ml) (Abbkine, PRP100051, USA) was added to the basal chambers for 3 days.
-
No products found
because this supplier's products are not listed.
Alessandro Gori, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The detector antibody (biotinylated CD9, CD63, CD81 antibodies by Ancell or anti-band 3 from Santa Cruz) solutions (0.3 µg/ml ...
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No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
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No products found
because this supplier's products are not listed.
Antje Neeb, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... panBAG-1 (human, rabbit monoclonal, RM356, RevMAb), panBAG-1 (mouse ...
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No products found
because this supplier's products are not listed.
John C. Newman, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Primary antibodies used were biotinylated 82E1 (1:1000; Immuno-Biological Laboratories) for Aβ plaques detection and rabbit anti-NPY (1:2000; Immunostar). NPY Primary antibodies were detected with biotinylated donkey anti-rabbit (1:500 ...
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No products found
because this supplier's products are not listed.
Alam García-Heredia, et al.,
bioRxiv - Microbiology 2020
Quote:
... lipid-linked peptidoglycan precursors were biotinylated by subjecting organic extracts to an in vitro PBP4-mediated exchange reaction with biotin-D-lysine (Chem-Impex International ...
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No products found
because this supplier's products are not listed.
Justine Cristante, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or pTer1 control vector (pTer1 cells)16 were authenticated by CellCheck human 16 Plus Test (9 human STR marker profile + 7 additional Human STR marker, Inter-species contamination Test and STAT-mycoplasma testing) (IDEXX Analytics). They were grown in Dulbecco’s Modified Eagle Medium:F-12 (DMEM/F-12 Glutamax ...
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No products found
because this supplier's products are not listed.
M Azharuddin, et al.,
bioRxiv - Immunology 2021
Quote:
... or anti-human IgA-HRP (Nordic BioSite, Täby, Sweden) was added to separate wells with diluted serum samples and incubated 90 min ...
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No products found
because this supplier's products are not listed.
Astrid Hendriks, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human neutrophils were freshly isolated using Polymorphprep (Alere technologies) per manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Jorge Gomez-Deza, et al.,
bioRxiv - Neuroscience 2023
Quote:
The Human Genome-Wide CRISPRi Dual-sgRNA Library (Cellecta KIDHGW-105K-P ...
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MCAT (180 aa) recombinant protein +GST-tag at NT.
Cat# HRP1007,
Inquiry
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Jared M. Fischer, et al.,
bioRxiv - Bioengineering 2024
Quote:
... A375-Luc/iRFP (human melanoma, Creative Biogene CSC-RR0254), MCF7 (human breast cancer ...
-
No products found
because this supplier's products are not listed.
Neetu Singh Kushwah, et al.,
bioRxiv - Molecular Biology 2021
Quote:
AAE3 protein sequences from Medicago truncatula (GenBank ...
-
No products found
because this supplier's products are not listed.
Helene Jahn, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Protein concentration was determined by SDS-PAGE (Coomassie or Fluorescence Protein gel stain (Lamda Biotech)) in comparison with standards ...
-
No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
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No products found
because this supplier's products are not listed.
Soumya Mukherjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Commercial pooled human plasma was purchased from Affinity Biologicals (Ancaster, ON). Purified human neutrophils ...
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No products found
because this supplier's products are not listed.
Jean-Louis A. Parmasad, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 500 µL 20 mg/mL human insulin (Wisent Bioproducts, 511-016-CM), 2.5 mL 1M HEPES (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Raeann Goering, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... with protein ladder (Gel Company FPL-008). Gels were imaged with a Sapphire molecular imager (Azure Biosystems ...
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No products found
because this supplier's products are not listed.
Yu-Yi Kuo, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The supernatants were collected and the protein concentration was quantified by the Bradford protein assay (BioShop Canada Inc., Burlington, Ontario, Canada). Protein samples were stored at −80°C for subsequent western blotting assessments.
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No products found
because this supplier's products are not listed.
Duilio M. Potenza, et al.,
bioRxiv - Physiology 2024
Quote:
... and human cardiac fibroblasts was extracted with Trizol Reagent (TR-118, Molecular Research Center) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Moon Kee Meang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein bands were visualized using ECL reagent (Abfrontier, Korea) and Uvitec HD9 (Uvitec ...
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No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
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No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Human B cell proliferation was measured by 3[H]-thymidine incorporation (1 μCi/mL) (American Radiolabeled Chemicals).
-
No products found
because this supplier's products are not listed.
Shivani Ahuja, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the protein was mixed 2:3 with monoolein (Nu-Chek Prep) and then 70 nl of this material was deposited on a glass slide (Molecular Dimensions) ...
-
No products found
because this supplier's products are not listed.
Adam Beck, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The antibodies and protein standards are commercially available from EnCor Biotechnology Inc.
-
No products found
because this supplier's products are not listed.
MaryAnn Martin, Irene L.G. Newton,
bioRxiv - Microbiology 2023
Quote:
Proteins were separated on 4-20% Tris-Glycine NB precast minigels (NuSep) and transferred to PVDF membrane in Tris-Glycine transfer buffer with 15% methanol at 40v on ice for 3 hours ...
-
Fluorescent Method
Cat# FPRO200,
FluoProdige fluorescent Dye (10ml) + Dilution Buffer (100ml) + BSA(500µg) lyoph, USD $229.00/KIT
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
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No products found
because this supplier's products are not listed.
Lennart Schada von Borzyskowski, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... all protein digests were desalted using C18 microspin columns (Harvard Apparatus, Holliston, USA) according to the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Marijn Knip, Emy Latul, Frank LW Takken,
bioRxiv - Plant Biology 2023
Quote:
Proteins were isolated from six leaf discs (5mm Ø World Precision Instruments (WPI)) ...
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No products found
because this supplier's products are not listed.
Paweł P. Knejski, et al.,
bioRxiv - Biochemistry 2023
Quote:
... purified proteins were diluted to 0.05 mg/mL and applied onto plasma-cleaned (Gatan Solarus) copper grids with a continuous carbon layer (Electron Microscopy Sciences) ...
-
No products found
because this supplier's products are not listed.
Hagit Hak, et al.,
bioRxiv - Plant Biology 2024
Quote:
... and images of protein bands were acquired and quantified using the Alliance Q9 software (UVITEC).