-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Suzanne G. Mays, et al.,
bioRxiv - Biochemistry 2021
Quote:
... expressing N-terminal Avi-tagged human LRH-1 and AAV8-GFP (used as a negative control) were obtained from Vector Biolabs (Malvern, PA, USA). C57BL/6J mice were administered 1×1012 genome copies (GC)/mL via femoral vein injection ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Joshua G. Pemberton, et al.,
bioRxiv - Cell Biology 2019
Quote:
... Angiotensin II (Human octapeptide; Bachem) was first dissolved in ethanol at 1mM before being prepared as 100 μM aliquots for storage by dilution with ddH2O water ...
-
No products found
because this supplier's products are not listed.
Muhammad Rizwan, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Recombinant protein G (Cedarlane Labs Inc). All solvents were purchased from Caledon ...
-
No products found
because this supplier's products are not listed.
Lucia Gonzalo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 4°C and the supernatants were incubated overnight with 1/100 RNAPII or HIS antibodies (RNAP II AS11 1804, HIS AS20 4441, Agrisera) and 30 μl SureBeads (BioRad) ...
-
No products found
because this supplier's products are not listed.
Michael J Abrams, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... and 0.1 mg/mL insulin (human recombinant, MP Biomedicals 0219390080). To introduce the molecular factors ...
-
No products found
because this supplier's products are not listed.
Nora-Guadalupe P. Ramirez, et al.,
bioRxiv - Microbiology 2021
Quote:
... 1 x 106 Jurkat cells expressing Strep-tagged ADAP1 or GFP were resuspended in 0.1 mL Mirusbio ingenio (Mirus, MIR50115) solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene ...
-
No products found
because this supplier's products are not listed.
Giuseppe Deganutti, et al.,
bioRxiv - Biochemistry 2021
Quote:
... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
-
No products found
because this supplier's products are not listed.
Liangxi Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... 10 ng/ml recombinant human TNFα (Cell Applications, cat# RP1111-50) was added for 3 hours ...
-
No products found
because this supplier's products are not listed.
Senthil-Kumar Devan, et al.,
bioRxiv - Microbiology 2022
Quote:
... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
-
No products found
because this supplier's products are not listed.
Chao Wang, et al.,
bioRxiv - Cell Biology 2019
Quote:
... recombinant human TNFa (Shanghai Primegene Bi-Tech) was coated onto microtiter plates (Greiner Bio-One) in 0.05M carbonate-bicarbonate buffer overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Krisztina Ötvös, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The purified recombinant protein was used to immunize rabbits by a company (Eurogentec). From the immunserum a crude IgG fraction was isolated by ammonium sulfate precipitation then IgG was further purified on protein gel blots of the antigen ...
-
No products found
because this supplier's products are not listed.
Hsuan Cheng, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The intensity of YFP-FKBP-tagged proteins at centrosomes upon rapamycin treatment was measured by Nikon Elements AR software.
-
No products found
because this supplier's products are not listed.
Xingxing Zhu, et al.,
bioRxiv - Immunology 2024
Quote:
... and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell) and 10 ng/mL recombinant mouse IL-4 ...
-
No products found
because this supplier's products are not listed.
Shivneet K. Gill, et al.,
bioRxiv - Biochemistry 2024
Quote:
Protein toxins were incubated with either human sera (Human Serum age 4-6, Innovative Research) or PBS in a 1:1 ratio ...
-
No products found
because this supplier's products are not listed.
Jiechao Zhou, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Purified human complement proteins (4 µg/mL, Complement Technology) were immobilized and were treated with His-tagged recombinant neuronal pentraxin proteins (4 µg/mL ...
-
No products found
because this supplier's products are not listed.
Asano Watanabe, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GFP-tagged human vWF plasmid (Romani de Wit et al., 2003) was kindly provided by Jan Voorberg (Stichting Sanquin Bloedvoorziening, Netherland) under Materials Transfer Agreement ...
-
No products found
because this supplier's products are not listed.
Kensuke Ninomiya, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... All MS/MS spectra were searched against protein sequences of the RefSeq (NCBI) human protein database (RDB) using the Protein Pilot software package (AB Sciex), and decoy sequences were then selected with a false discovery rate <1%.
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No products found
because this supplier's products are not listed.
Peng Dong, et al.,
bioRxiv - Genomics 2022
Quote:
... Mouse embryonic stem cells were plated on Human recombinant laminin 511-coated coverslips (Electron Microscopy Sciences, Cat. 72196-25). Cells were then fixed using 4% formaldehyde (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Ben Johnson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
-
No products found
because this supplier's products are not listed.
Yang Xu, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
-
No products found
because this supplier's products are not listed.
Pablo Lazcano, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Corresponding europium-tagged secondary antibodies (Molecular Devices) were used at a 1:5000 dilution and 1:20,000 for actin ...
-
No products found
because this supplier's products are not listed.
Emanuel Schmid-Siegert, et al.,
bioRxiv - Genomics 2023
Quote:
... Samples were sequenced with Sequel II Sequencing Kit 2.0 on PacBio Sequel II (PacBio).
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
-
No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
-
No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
-
No products found
because this supplier's products are not listed.
Francesca Curreli, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... Various concentrations of biotinylated recombinant SARS-CoV-2 Spike RBD protein (Bioss Antibodies, Woburn, MA) dissolved in water were manually printed onto the Chip with Biodo bioprinting at 40% humidity via biotin-avidin conjugation ...
-
No products found
because this supplier's products are not listed.
Chieh-Yu Liang, et al.,
bioRxiv - Immunology 2024
Quote:
Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Belinda Wang, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 15 μg of Strep-tagged plasmids was combined with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid:transfection reagent ...
-
No products found
because this supplier's products are not listed.
Moumita Sarkar, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... pCDNA-T292A/S294A and pCDNA-Cnot3 Δ651-751 constructs were in vitro transcribed/translated as described above and the resultant V5 tagged proteins were purified using V5-tagged protein purification kit ver.2 (MBL International, USA). Purified proteins were used as substrate and incubated with 80 ng of GST-Aurora B (ProQuinase ...
-
No products found
because this supplier's products are not listed.
Lorenz Thurner, et al.,
bioRxiv - Immunology 2021
Quote:
... or by biotinylated anti-human IgG antibody (Dianova#109-066-097) followed by Strep/Pox 1:15000 (Roche#11089153001 ...
-
No products found
because this supplier's products are not listed.
Huang Zhu, Dan S. Kaufman,
bioRxiv - Immunology 2019
Quote:
... 15 % heat-inactivated human AB serum (Valley Biomedical, Catalog # HP1022 HI),1 % P/S ...
-
No products found
because this supplier's products are not listed.
Nawar Naseer, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 50 ng/ml recombinant human M-CSF (Gemini Bio-Products) for 6 days to promote differentiation into hMDMs ...
-
No products found
because this supplier's products are not listed.
Hugo Girão, et al.,
bioRxiv - Cell Biology 2019
Quote:
... using NZYColour Protein Marker II (NZYTech). Blotting was performed with an iBlot Gel Transfer System (Invitrogen) ...
-
No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
-
No products found
because this supplier's products are not listed.
Yoji Tsugawa, et al.,
bioRxiv - Microbiology 2024
Quote:
... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Patrik Risteski, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and human anti-centromere protein antibody (Antibodies Incorporated). The secondary antibodies used were donkey anti-mouse IgG-Alexa Fluor 488 (Abcam) ...
-
No products found
because this supplier's products are not listed.
Jakob Berner, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 1% Pen/strep (Pan Biotech), 1 mM sodium pyruvate (Pan Biotech) ...
-
No products found
because this supplier's products are not listed.
Inga V. Hochheiser, et al.,
bioRxiv - Immunology 2021
Quote:
The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
-
No products found
because this supplier's products are not listed.
Ella F.J. Halcrow, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HeLa cells expressing the different GFP-tagged proteins were plated on an open μ-Slide with 8 wells (Ibidi, 80826) in complete medium containing 0.5 μM SiR-DNA dye (SpiroChrome) ...
-
No products found
because this supplier's products are not listed.
Anne Mohrholz, et al.,
bioRxiv - Plant Biology 2019
Quote:
... the equal content of GFP-tagged fusion protein in the crude extracts was adjusted according to the GFP fluorescence using a fluorescence reader (TECAN Safire).
-
No products found
because this supplier's products are not listed.
Kevin P. Foley, et al.,
bioRxiv - Physiology 2020
Quote:
... Human insulin (Mercodia) and human C-peptide (Millipore ...
-
No products found
because this supplier's products are not listed.
Federico Pelisch, et al.,
bioRxiv - Cell Biology 2019
Quote:
... a Cascade II camera (Photometrics), spinning-disk head (CSU-X1 ...
-
No products found
because this supplier's products are not listed.
Sisi Zhang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... under a Zeiss Axioskop II microscope (Carl Zeiss). The sections were counterstained with the Mayer’s hematoxylin ...