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No products found
because this supplier's products are not listed.
Matthew J. Stevenson, et al.,
bioRxiv - Cancer Biology 2022
Quote:
DNA sequences flanked by XhoI and NdeI restriction sites and encoding for N-terminal GST-tagged-SIX1 or N-terminal GST-tagged-SIX1-Q177R homeodomains were synthesized by Integrative DNA Technologies (IDT) as gBlocks (Supplemental Table 2) ...
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No products found
because this supplier's products are not listed.
Omnia Reda, et al.,
bioRxiv - Microbiology 2023
Quote:
... The amount of p24 Gag in the concentrated virus was quantified using an HIV-1 p24 antigen enzyme-linked immunosorbent assay (ELISA) kit (ZeptoMetrix, Cat# 0801002) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Natasha Ting Lee, et al.,
bioRxiv - Neuroscience 2023
Quote:
Recombinant mouse t-PA (0.9 mg/kg; Molecular Innovations, MI, USA) or saline (equi-volume ...
-
No products found
because this supplier's products are not listed.
Ekta Makhija, et al.,
bioRxiv - Biophysics 2022
Quote:
... Type II Collagen (Col 2) content was measured using a captured enzyme-linked immunosorbent assay (Chondrex, Redmond, WA). Absorbance at 490 nm was measured and the concentration of Col 2 was extrapolated from a standard curve generated using a Col 2 standard ...
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No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... Purified recombinant viral proteins were solubilized in PBS and biotinylated chemically with sulfo-NHS-LC-biotin (sulfo-NHS-LC-biotin, Proteochem, USA) at a molar ratio of 1:1 ...
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No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were dispensed at various volumes into human ASC-coated 1,536-well plates using contactless dispensing with an ECHO 555 Acoustic Dispenser (Labcyte). Thereby ...
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magnetofection
difficult to transfect cells
Cat# KC30296,
PolyMag 100µL+PolyMag Neo100µL+ CombiMag 100µL+Magnetic Plate MF96000, USD $640.63/KIT
Ask
Alexandra Grubman, et al.,
bioRxiv - Neuroscience 2019
Quote:
... were transfected for 48 h with dox-inducible GFP-tagged Gateway-generated Piggybac expression constructs with inserted cDNA encoding human HIF1A and/or ELF3 open reading frames using Glial Mag transfection kit (Oz Biosciences, #GL00500) according to manufacturer’s instructions ...
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Cat# EP295-1,
USD $450.0/mg
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Hanieh Ghassabian, et al.,
bioRxiv - Microbiology 2020
Quote:
... α-UL44 mAb (Virusys Corporation, #P1202-1; 1:100); α-pp65 mAb (Virusys Corporation ...
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No products found
because this supplier's products are not listed.
Katy R. McCarron, et al.,
bioRxiv - Cell Biology 2023
Quote:
... anti-LC3 (1:200 WB, 1:200 IF, Nanotools; 5F10), anti-p62 (1:1,000 WB ...
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No products found
because this supplier's products are not listed.
Sarah L. Olguin, et al.,
bioRxiv - Neuroscience 2020
Quote:
... NbActive-1 medium (BrainBits) supplemented with 100 U/ml of Penicillin-Streptomycin (Life Technologies ...
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No products found
because this supplier's products are not listed.
Dinh Thi Nguyen, et al.,
bioRxiv - Cell Biology 2021
Quote:
... EthD-1 (2μM) and Hoechst 33342 (1 μg/ml, Dojindo Molecular and Technologies Inc.) in regular medium ...
-
No products found
because this supplier's products are not listed.
Kerstin K. Schwickert, et al.,
bioRxiv - Cell Biology 2024
Quote:
... anti-dsRNA (SCICONS, 1:200), anti-pORF2 (HCD3K129 ...
-
No products found
because this supplier's products are not listed.
Wenwei Li, et al.,
bioRxiv - Microbiology 2021
Quote:
... and 1 mM dithiothreitol) and 50 μL of 1 mM D-luciferin potassium salt (Prolume). The neutralization half-maximal inhibitory dilution (IC50 ...
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No products found
because this supplier's products are not listed.
Hammam Antar, Stephan Gruber,
bioRxiv - Microbiology 2023
Quote:
... Equal volumes of each solution were mixed together (protein:ligand 1:1) using BenchSmart 96 (Rainin) dispenser robot and mixed through pipetting ...
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No products found
because this supplier's products are not listed.
Jonathon A.B. Smith, et al.,
bioRxiv - Physiology 2024
Quote:
... and glucose uptake was measured in the same medium by adding 1 uL.mL-1 of 2-[1,2-3H]Deoxy-D-glucose (MT911, Moravek) and 10 μM unlabelled 2-Deoxy-D-glucose in for 15 min [32] ...
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No products found
because this supplier's products are not listed.
Marta Urbanska, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1 mM EDTA) using 1 ml Float-A-Lyzer G2 Dialysis Device with molecular cutoff of 1000 kDa (G235037, Spectrum Labs). The dialysis buffer was exchanged 5 times with 4–16 h intervals.
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No products found
because this supplier's products are not listed.
D.E. Selley, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... Bilateral tissue punches 1–1.5 mm in length were taken with a 1 mm internal diameter corer (Fine Science Tools; Foster City, CA) and placed in Eppendorf tubes kept on dry ice and then stored at −80° C ...
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No products found
because this supplier's products are not listed.
Hyun Ji Kim, et al.,
bioRxiv - Genomics 2022
Quote:
... The particle concentration of each plasma fraction was determined by converting the blockade frequency of each plasma fraction using Izon Control Suite (v3.3.2.2001, Izon Science). Plasma protein absorbance was measured at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific ...
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No products found
because this supplier's products are not listed.
Siamak Salavatian, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 1 µL of an enzyme solution containing 1 U glutamate oxidase (Cosmo Bio USA, Carlsbad, CA, USA), 13.7 mg bovine serum albumin (Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Jennyfer M. Mitchell, Scott A. Nichols,
bioRxiv - Evolutionary Biology 2019
Quote:
... His-EmFAK and GST-EmITGB1 (Syd Labs) recombinant proteins ...
-
No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
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No products found
because this supplier's products are not listed.
Madelon M.E. de Jong, et al.,
bioRxiv - Immunology 2023
Quote:
... slides were incubated with anti-human CD15 (1:10, C3D-1, Diagnostic BioSystems) for 1 hour at 37°C ...
-
No products found
because this supplier's products are not listed.
Theresa E. Mackey, et al.,
bioRxiv - Physiology 2020
Quote:
... Plasma cortisol levels were quantified using an enzyme-linked immunosorbent assay (ELISA; 1:50 dilution; Neogen Corporation, Lexington, Kentucky, USA), previously validated for use in other salmonids (e.g. ...
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No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
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No products found
because this supplier's products are not listed.
Tetsuro Yamamoto, et al.,
bioRxiv - Immunology 2023
Quote:
... HRP-human IgG antibody (EY Laboratories, USA), and BT IgE antibody (Bio-Rad Laboratories ...
-
No products found
because this supplier's products are not listed.
Yuichi Mitsui, et al.,
bioRxiv - Immunology 2022
Quote:
Human PBMCs were purchased from Precision for Medicine, Inc ...
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No products found
because this supplier's products are not listed.
M Azharuddin, et al.,
bioRxiv - Immunology 2021
Quote:
... or anti-human IgA-HRP (Nordic BioSite, Täby, Sweden) was added to separate wells with diluted serum samples and incubated 90 min ...
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No products found
because this supplier's products are not listed.
Ava P. Soleimany, et al.,
bioRxiv - Bioengineering 2020
Quote:
Fresh frozen human PCa TMAs (BioChain Institute, Inc.; T6235201) were air dried and fixed in ice-cold acetone for 10 minutes ...
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No products found
because this supplier's products are not listed.
Takao Fujisawa, et al.,
bioRxiv - Cell Biology 2023
Quote:
Recombinant proteins or ZnCl2 solution for normalization was incubated with 10 µM ZnAF-2 (Goryo Chemical, SK2001-01) in TBS for 30 min at room temperature ...
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No products found
because this supplier's products are not listed.
Disha Sood, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... Hyaluronan and chondroitin sulfate present within the samples were digested using the following enzymes: chondroitinase ABC (Seikagaku 25mU/ml) and hyaluronidase SD (Seikagaku 2.5mU/ml) ...
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No products found
because this supplier's products are not listed.
Xuan He, et al.,
bioRxiv - Immunology 2021
Quote:
... The plates were washed with coulter buffer and incubated for 2 h with Rabbit polyclonal anti-human IFN-γ Biotin from U-Cytech (1 μg/mL). The plates are washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase antibody from Southern Biotechnology (1 μg/mL) ...
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No products found
because this supplier's products are not listed.
Robin Hoeven, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
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No products found
because this supplier's products are not listed.
Soumya Mukherjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Commercial pooled human plasma was purchased from Affinity Biologicals (Ancaster, ON). Purified human neutrophils ...
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No products found
because this supplier's products are not listed.
Aaron M. Rosado, et al.,
bioRxiv - Biophysics 2022
Quote:
... freshly isolated human RBCs were biotinylated with biotin-PEG3500-NHS (Jenkem Technology) and then incubated with nystatin in N2 buffer (265.2 mM KCl ...
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No products found
because this supplier's products are not listed.
Joe Jongpyo Lim, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The tubes with the livers and enzyme mix were incubated at 37 °C for 40 minutes using a Roto-Therm H2020 incubator (Benchmark Scientific Inc., Sayreville, NJ). After the incubation ...
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No products found
because this supplier's products are not listed.
Qing Zhao, et al.,
bioRxiv - Immunology 2023
Quote:
... and then incubated with Dylight 550 Goat anti-human IgA (ImmunoReagents, Cat # GtxHu-001-E550NHSX) and Dylight 650 Donkey anti-human IgG (Invitrogen ...
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No products found
because this supplier's products are not listed.
Sudeep Sarma, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Peptides SA1-SA4 for functional testing on human gut epithelium were synthesized on Wang resin pre-loaded with the first amino acid on an Initiator+ Alstra (Biotage, Uppsala, Sweden) following the Fmoc/tBu protecting strategy ...
-
No products found
because this supplier's products are not listed.
Nicholas N. Foster, et al.,
bioRxiv - Neuroscience 2020
Quote:
... fluorogold (FG, 1%; Fluorochrome); and cholera toxin subunit B-AlexaFluor 647 conjugate (CTB ...
-
No products found
because this supplier's products are not listed.
Akash D. Chakraborty, et al.,
bioRxiv - Physiology 2023
Quote:
... SERCA2 (1:5000, Badrilla), CSQ2 (1:3000 ...
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No products found
because this supplier's products are not listed.
Sangeeta Ghuwalewala, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Mice were fed with doxy chow (1 g doxy/1 kg, Bio-serv) for the indicated chase periods ...
-
No products found
because this supplier's products are not listed.
Nicholas D. LeBlond, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 1 L (Bioshop Canada - CCL402.1), Chloroquine (Sigma-Aldrich - C6628-25G) ...
-
No products found
because this supplier's products are not listed.
Tiphaine Péresse, et al.,
bioRxiv - Cell Biology 2019
Quote:
... 1% antibiotics (Zell Shield, Minerva Biolabs) and were incubated at 37°C in a 5% CO2 humidified atmosphere ...
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No products found
because this supplier's products are not listed.
Wenyang Yi, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Sheep anti VSX2 (1:400, Exalpha Biologicals), Sheep anti ONECUT2 (1:40 ...
-
No products found
because this supplier's products are not listed.
Serena R.G. Page, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 1 mL/L of B5 vitamins (Phytotechnology Laboratories), and 0.5 μM TDZ (Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Francesco Pesce, et al.,
bioRxiv - Biophysics 2022
Quote:
... 1 mM DTT) and cells lysed by 1 cycle of French Press at 25 kPsi (Constant Systems Ltd MC Cell Disrupter). The lysate was centrifuged at 4 °C and 20000 g for 30 min and the supernatant applied to a gravity flow column with 4 mL pre-equilibrated Ni-NTA Sepharose resin (GE Healthcare) ...
-
No products found
because this supplier's products are not listed.
Nicholas E. Albrecht, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cysteamine hydrochloride (MEA, 1 M, Chem-Impex International #02839), and 2-mercaptoethanol (BME ...
-
No products found
because this supplier's products are not listed.
Taylor Anthony Stevens, et al.,
bioRxiv - Biochemistry 2023
Quote:
... vented cap (1 L roller bottle; Celltreat, cat. no. 229383)
-
No products found
because this supplier's products are not listed.
Caroline Murawski, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and 2 µm S1818 (Microchem, 5000 rpm, baking at 100°C for 1 min). Patterns were developed for 40 – 50 s in MF319 (Microchem) ...
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No products found
because this supplier's products are not listed.
Eric Vallabh Minikel, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Samples were injected (1 to 2 µg) onto a 75 um ID PicoFrit column (New Objective) packed to 20 cm with Reprosil-Pur C18 AQ 1.9 um media (Dr ...