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No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
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No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
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No products found
because this supplier's products are not listed.
Matthew R. McFarland, et al.,
bioRxiv - Systems Biology 2020
Quote:
... HA-tagged Gln4 protein was detected using an anti-HA antibody (HA.11 clone 16B12, Cambridge Biosciences) and SuperSignal West Femto substrate (Thermo Scientific ...
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No products found
Minghui Di, et al.,
bioRxiv - Molecular Biology 2023
Quote:
HeLa cells were transfected with DNA (1 ug) expressing mCherry-tagged proteins cultured on 29-mm glass-bottom dish (Cellvis); plasmids (1 ug ...
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No products found
because this supplier's products are not listed.
Alex Hunt, et al.,
bioRxiv - Microbiology 2019
Quote:
... The shortCAP recombinant protein was used to immunize female New Zealand white rabbits (Covalab, Cambridge, United Kingdom) for generation of polyclonal antibodies.
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No products found
because this supplier's products are not listed.
Clàudia Salat-Canela, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
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No products found
because this supplier's products are not listed.
José Ursic-Bedoya, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and protein quantification was assayed using human FGF19 ELISA kit (Biovendor, RD191107200R). Protein was then diluted 1:2 in PBS+0.2%BSA and stored at - 20°C.
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No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit, Cell Sciences, Inc., Cat# CKH113).
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No products found
because this supplier's products are not listed.
Su Z. Hong, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Viral injection of the recombinant adeno-associated virus expressing Cre recombinase under the control of human synapsin promoter (rAAV-hSyn-Cre; from SignaGen Laboratories, Rockville, MD) was done by bulk regional viral injection to the visual cortical area of neonatal Cre dependent Gs-DREADD mice at p1 ...
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No products found
because this supplier's products are not listed.
Dorothea Höpfner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... samples containing 100 ng recombinant protein were run over a 5 μm Jupiter C4 300Å LC column (Phenomenex, Torrance, California) using the 1260 Infinity LC system (Agilent Technologies ...
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No products found
because this supplier's products are not listed.
Mariano Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
The full-length PfS1 recombinant purified protein was used to immunized rabbits subcutaneously following a two-months standard procedure (Proteogenix, France). Briefly ...
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Recombinant Antigen
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Fred D. Mast, et al.,
bioRxiv - Biochemistry 2021
Quote:
Recombinant Fc-tagged SARS-CoV-2 Spike S1 and S2 proteins purified from HEK293 cells were used for llama immunization (The Native Antigen Company REC31806 and REC31807). For affinity isolation ...
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No products found
because this supplier's products are not listed.
Jan-Renier A.J. Moonen, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Flag-tagged KLF4 (#VH829440, Vigene Biosciences) or GFP control (AVP004, GenTarget Inc) for 12 h after which cells were allowed to recover for 90 h before being harvested for subsequent experiments.
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No products found
because this supplier's products are not listed.
Xiaoxuan Su, et al.,
bioRxiv - Microbiology 2021
Quote:
... Full length RNase L gene was synthesized and subcloned into pGEX-4T-3 vector (pGEX-4T-RNaseL-GST) by GENEWIZ as previously described (27).
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No products found
because this supplier's products are not listed.
Jeanette M. Metzger, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Nuclear localization signal (NLS)-tagged Streptococcus pyogenes Cas9 nuclease (sNLS-SpCas9-sNLS) was obtained from Aldevron. In vitro transcribed single guide RNAs (sgRNAs ...
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No products found
because this supplier's products are not listed.
Firyal Ramzan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkyne-tagged fatty acid analogs (alkyne-myristate (13-tetradecylnoic acid; 13-TDYA; Click Chemistry Tools 1164) or alkyne-palmitate (15-hexadecynoic acid ...
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No products found
because this supplier's products are not listed.
Wiktoria Ogrodzińska, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
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No products found
because this supplier's products are not listed.
Balaji Karthick Subramanian, et al.,
bioRxiv - Pathology 2019
Quote:
... Human primary podocytes from Celprogen Inc ...
-
No products found
because this supplier's products are not listed.
Ok-Hee Kim, et al.,
bioRxiv - Physiology 2019
Quote:
... and human intact FGF23 (Elabscience) were measured using enzyme-linked immunosorbent assay ...
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No products found
because this supplier's products are not listed.
Antonin Tutter, et al.,
bioRxiv - Biochemistry 2024
Quote:
... the indicated concentrations of purified 6His-tagged BRD4 were spotted onto blank nitrocellulose-coated glass slides (Grace Bio-Labs PATH protein microarray slides ...
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No products found
because this supplier's products are not listed.
Aruna Pal, Abantika Pal, Pradyumna Baviskar,
bioRxiv - Genomics 2020
Quote:
... Gene fragment insert in the recombinant plasmid was sequenced by an automated sequencer (ABI prism) using the dideoxy chain termination method with T7 and SP6 primers (Chromous Biotech ...
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No products found
because this supplier's products are not listed.
Jack A. Bryant, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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No products found
because this supplier's products are not listed.
Nicholas B. Karabin, et al.,
bioRxiv - Bioengineering 2020
Quote:
... Pooled human plasma was acquired from Zen-Bio Inc ...
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No products found
because this supplier's products are not listed.
Khairunnisa Mentari Semesta, et al.,
bioRxiv - Cell Biology 2020
Quote:
sgRNA transduced cells seeded on 6-well plates at ~60% confluency were transfected with 2 μg flag-tagged β2-AR for 48 hr using JetPrime Transfection reagent (Genesee, Cat #55134) following manufacturer’s protocols ...
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No products found
because this supplier's products are not listed.
Lars Gruber, et al.,
bioRxiv - Biochemistry 2023
Quote:
Monoculture and biculture spheroids of CCD-1137Sk human fibroblasts and HT-29 human colon cancer cells (both LGC Standards, Wesel, Germany) were prepared ...
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No products found
because this supplier's products are not listed.
Belen Esquerra-Ruvira, et al.,
bioRxiv - Molecular Biology 2022
Quote:
NZYBlue Protein Marker (NZYtech) was used for protein size estimation and protein concentrations were measured with QUBIT® 2.0 (Invitrogen).
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No products found
because this supplier's products are not listed.
F Abou Azar, et al.,
bioRxiv - Biochemistry 2022
Quote:
... were isolated from wildtype mice or transgenic mice over-expressing a TAP-tagged 14-3-3ζ that were fed a low-fat (LFD) or 60% high-fat diet (HFD; Research Diets, New Brunswick, NJ) for 12 weeks (24) ...
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No products found
because this supplier's products are not listed.
Akira Kitamura, et al.,
bioRxiv - Biophysics 2023
Quote:
... FCS measurements of Venus and Venus-tagged TDP-43 in the cell lysates were performed using an LSM510 META ConfoCor2 system (Carl Zeiss, Jena, Germany) equipped with a C-Apochromat 40×/1.2NA W Korr ...
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No products found
because this supplier's products are not listed.
Nadine R King, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 2 mg/ml Human Serum Albumin (HSA; Irvine Scientific), 10 μg/ml insulin (Sigma) ...
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No products found
because this supplier's products are not listed.
Aleksandr Andriianov, et al.,
bioRxiv - Microbiology 2023
Quote:
... Wells were screened by PCR for the presence of lysate with recombinant phages and T3Δ0.3 was further purified from individual plaques and verified by Sanger sequencing (Evrogen).
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No products found
because this supplier's products are not listed.
Andrew S. McNeal, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... human melanocytes were seeded into 6-well plates (Sarstedt, 83.3920) at 100,000 cells/well and either live-imaged or serially imaged as indicated ...
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No products found
because this supplier's products are not listed.
Qinghui Wang, et al.,
bioRxiv - Immunology 2022
Quote:
... Naïve CD3 human T cells were purchased from HemaCare (Lot #21068415). N/TERT-1 cells were a gift from the Rheinwald Lab (Dickson et al. ...
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No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
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No products found
because this supplier's products are not listed.
Aloysius Wong, et al.,
bioRxiv - Plant Biology 2019
Quote:
The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
-
No products found
because this supplier's products are not listed.
Yeongjoon Lee, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
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No products found
because this supplier's products are not listed.
Seren Hamsici, Gokhan Gunay, Handan Acar,
bioRxiv - Bioengineering 2022
Quote:
... Fmoc protected amino acids (Gyros Protein Technologies) were removed through treatment with 20% piperidine/DMF solution for 45 min (three times for 15 min ...
-
No products found
because this supplier's products are not listed.
Federica Lucini, et al.,
bioRxiv - Genomics 2024
Quote:
... Individual replicates are reported for 4f-SAMMY-seq experiments on human fibroblasts (3M, 3 million ...
-
No products found
because this supplier's products are not listed.
Lien D. Nguyen, et al.,
bioRxiv - Neuroscience 2022
Quote:
Total protein was extracted using RIPA buffer (Boston Bioproducts) supplemented with Complete ...
-
No products found
because this supplier's products are not listed.
Claire S. Chung, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... protein samples were spotted on carbon-coated Formvar grid (Ted Pella). The samples were stained with nanoW/uranyl acetate before air drying ...
-
No products found
because this supplier's products are not listed.
Ghulam Destgeer, et al.,
bioRxiv - Bioengineering 2020
Quote:
... particles were incubated with varying concentrations of human recombinant NT-proBNP (HyTest, Finland) for 1 hr with subsequent washing ...
-
No products found
because this supplier's products are not listed.
Shengnan Zheng, et al.,
bioRxiv - Cell Biology 2021
Quote:
The binding between recombinant proteins and lipids was evaluated using a lipid composition of 90% PC (egg, chicken; 840051; Avanti Polar Lipids) and 10% PS (Brain,840032p;Avanti Polar lipids ...
-
No products found
because this supplier's products are not listed.
Lindsey R. Conroy, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Recombinant PNGaseF Prime was obtained from Bulldog Bio, Inc ...
-
No products found
because this supplier's products are not listed.
Kenji Shimada, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... then 0.002 U of recombinant hOGG1 (Trevigen, 4130-100-E) was added to one sample and further incubated for 30 min at 37°C ...
-
No products found
because this supplier's products are not listed.
Tobias Tertel, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 12 nM anti-human CD63 PE (EXBIO) or 13 nM anti-human CD81 PE (Beckman Coulter) ...
-
No products found
because this supplier's products are not listed.
Daria A. Egorova, et al.,
bioRxiv - Microbiology 2022
Quote:
... Total protein quantity was measured with QuDye Protein kit (Lumiprobe) on Qubit fluorometer (ThermoScientific).
-
No products found
because this supplier's products are not listed.
JM Robinson, et al.,
bioRxiv - Immunology 2019
Quote:
... I-FABP (Human I-FABP ELISA Kit, Hycult biotech, Cat# HK406), and LBP (Human Lipopolysaccharide Binding Protein ELISA Kit ...
-
No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Crystallisation of recombinant Cys232Ser α1-antitrypsin was carried out in 2-well MRC crystallisation plates with a Mosquito robot (TTP Labtech) using 100nl protein solution and 100nl well solution ...
-
No products found
because this supplier's products are not listed.
Kristine L Trotta, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein was transferred to nitrocellulose (0.2µm; GVS) via semi-dry transfer with a TransBlot Turbo transfer system (BioRad ...
-
No products found
because this supplier's products are not listed.
Mingyu Fang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... gels were either stained with Coomassie (Protein Ark) or transferred to PVDF membranes for Western blot analysis.
-
No products found
because this supplier's products are not listed.
Prasath Paramasivam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... and contained CleanCap® Enhanced Green Fluorescent Protein (eGFP) mRNA (5-methoxyuridine)and/or Clean Cap®Cyanine5 (Cy5) Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) (TriLink Biotechnologies). Formaldehyde (Merck) ...