-
No products found
because this supplier's products are not listed.
Ryan J. Garrigues, et al.,
bioRxiv - Microbiology 2021
Quote:
Purified BBK32-C and a purified autolytic fragment of human C1r (Complement Technologies) were mixed at 5% molar excess BBK32-C and concentrated to 3.5 mg ml-1 in 10 mM HEPES (pH 7.3 ...
-
No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
-
No products found
because this supplier's products are not listed.
Denise Jahn, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant mouse Cgrp (Bachem #H-2265, 10-7M).
-
No products found
because this supplier's products are not listed.
Adam J. Rauckhorst, et al.,
bioRxiv - Biochemistry 2023
Quote:
... injection with 13C-glucose (10% 13 C-glucose, 2.0g/kg lean body mass, Cambridge Isotope) or 13C-lactate/13C-pyruvate (10:1 ...
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No products found
because this supplier's products are not listed.
Kyla D Omilusik, et al.,
bioRxiv - Immunology 2021
Quote:
... splenocytes were incubated for 5 hours at 37°C in RPMI-1640 media containing 10% (v/v) bovine growth serum with 10 nM GP33-41 peptide (Anaspec) and Protein Transport Inhibitor (eBioscience ...
-
No products found
because this supplier's products are not listed.
Nasim Saadat, et al.,
bioRxiv - Genetics 2024
Quote:
Initial small crystals were formed at 4 °C in 3.5 M sodium formate (Condition X of Index Screen; Hampton Research) in a sitting drop format as part of a robot-controlled screen in 96 well plates (200 nL drop size ...
-
No products found
because this supplier's products are not listed.
Michael A. Kowalski, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and 1% penicillin-streptomycin-fungizone [PSF]) or basal media supplemented with 10 ng/mL of IL-1β (human recombinant; Tonbo Bioscience 218018U010) for 2 weeks ...
-
No products found
because this supplier's products are not listed.
Gurur Garip, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... anti-c-Caspase3 (Bioss BS-0081R), anti-Beclin1 (S-1353R ...
-
No products found
because this supplier's products are not listed.
Frédéric Frottin, et al.,
bioRxiv - Biochemistry 2020
Quote:
... transfected cells were treated with 20 ng/ml recombinant human TNFα (Jena Biosciences) for 30 min ...
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No products found
because this supplier's products are not listed.
Shiwei Liu, et al.,
bioRxiv - Genomics 2023
Quote:
... we grew parasites at 37°C in vitro at 3% hematocrit (serotype A positive human erythrocytes, Valley Biomedical, VA or BioIVT, NY) in RPMI 1640 medium (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Andrew LaPoint, et al.,
bioRxiv - Physiology 2023
Quote:
... and cholesterol (2%) (HFHF-C, Research Diets, D09100310) diet or a matched sucrose ...
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No products found
because this supplier's products are not listed.
Kevin O. Saunders, et al.,
bioRxiv - Immunology 2023
Quote:
... Env trimers with C-terminal avi-tags (Avidity) were biotinylated and conjugated to streptavidin labeled with different fluorochormes ...
-
No products found
because this supplier's products are not listed.
Zak Frentz, Jonathan Dworkin,
bioRxiv - Biophysics 2020
Quote:
... through sterile tubing (Cole-Parmer, C-Flex #13, i.d. 0.8 mm), into the perfusion chamber ...
-
No products found
because this supplier's products are not listed.
Rosa Machado, et al.,
bioRxiv - Biophysics 2019
Quote:
... we warmed the objective to 37 °C using an objective heater (Bioptechs). In some experiments we used a custom-made microscope incubation chamber ...
-
No products found
because this supplier's products are not listed.
Paul D. Simonson, Itzel Valencia, Sanjay S. Patel,
bioRxiv - Immunology 2022
Quote:
... The standard Akoya CODEX protocols were followed (Akoya CODEX manual, revision C). For tyramide-barcode staining ...
-
No products found
because this supplier's products are not listed.
Carole Lartigue, et al.,
bioRxiv - Microbiology 2021
Quote:
... They were observed at −178 °C using a cryo holder (626, Gatan), with a ThermoFisher FEI Tecnai F20 electron microscope operating at 200 kV under low-dose conditions ...
-
No products found
because this supplier's products are not listed.
Annemarie Lang, et al.,
bioRxiv - Bioengineering 2024
Quote:
... KS) and moved onto a heating pad (37 °C; Kent Scientific, Torrington, CT). Anesthesia was maintained at ∼1.5–2% with an individual a nose cone ...
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No products found
because this supplier's products are not listed.
Neuza S. Sousa, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Recombinant human MANF (P-101–100; Icosagen) was used as a standard ...
-
No products found
because this supplier's products are not listed.
Shawn M. Costello, et al.,
bioRxiv - Biophysics 2021
Quote:
... and packed by hand into a column (2mm ID x 2 cm, IDEX C-130B). Following digestion ...
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No products found
because this supplier's products are not listed.
Maxime Lecerf, et al.,
bioRxiv - Immunology 2020
Quote:
... Biotinylated recombinant human FcRn (Amsbio LLC, Cambridge, MA) was diluted to 5 μg/ml in running buffer - 100 mM Tris pH 6 (adjusted by addition of dry citric acid) ...
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No products found
because this supplier's products are not listed.
Wu Liu, et al.,
bioRxiv - Microbiology 2019
Quote:
... 2’-C-methyladenosine (2’-C-Me-A) was obtained from Carbosynth Limited (Compton ...
-
No products found
because this supplier's products are not listed.
Ida Søgaard Larsen, et al.,
bioRxiv - Microbiology 2023
Quote:
... and C-peptide by Mouse C-peptide ELISA kit (Crystal Chem) following the manufacturers’ protocols.
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... and 50ng/ml recombinant human IL-18 (MBL International) were added to the IL-15 cultures for the last 12 hrs (overnight stimulation).
-
No products found
because this supplier's products are not listed.
Tomoyuki Hatano, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The plates were incubated for two days at 30 □C before the X-Gal (#RC-212, G-Biosciences, USA) overlay ...
-
No products found
because this supplier's products are not listed.
Seong Beom Ahn, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the peptide trap was switched in-line with a cHiPLC C18 column (15 cm x 200μm, 3μm, ChromXP C18-CL, 120 Å, 25°C, SCIEX) and peptides were eluted using a linear 120min gradient from 5 % acetonitrile to 35 % mobile phase B (B ...
-
No products found
because this supplier's products are not listed.
Frederik Fleissner, et al.,
bioRxiv - Biophysics 2020
Quote:
A plasmid coding for human vimentin containing a C-terminal His-tag (EX-D0114-B31, Tebu-bio, Germany) was transformed into E ...
-
No products found
because this supplier's products are not listed.
Li Sun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... at 95°C for 10 minutes and alkylated with 40 mM iodoacetamide (Amresco, RPN6302V, IAA) at room temperature in dark for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Minghao Chen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
-
No products found
because this supplier's products are not listed.
Francesco Maura, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... followed by protease treatment at 40º C for 10-15 minutes (Abbott Molecular, Des Plaines, IL), then by dehydrating 2 minutes each in a serios of 70% ...
-
No products found
because this supplier's products are not listed.
Luc Roberts, Hans-Joachim Wieden,
bioRxiv - Biochemistry 2021
Quote:
... To quench the reaction 1.5 μL of 0.5 M EDTA pH 8.0 was added and the reaction subsequently heated to 75°C for 10 min before the RNA was purified via EZ-10 Spin Column RNA Cleanup and Concentration Kit (Bio Basic).
-
No products found
because this supplier's products are not listed.
Matthew L. Baum, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Slides were blocked for 1hr in blocking solution (antibody buffer with 5% BSA and 0.8% Triton X-100) followed by incubation overnight at 4°C with 1:1000 rabbit anti-Csmd1 antibody (Lund, produced by Agrisera, Sweden) in 4:1 antibody buffer (150 mM NaCl ...
-
No products found
because this supplier's products are not listed.
Xiangling Meng, et al.,
bioRxiv - Neuroscience 2022
Quote:
... samples were washed with PBS (2 hours x 3, 37°C) before incubation with the Tissue-Clearing Reagent CUBIC-L (TCI, T3740) at 37°C for ∼18 hours ...
-
No products found
because this supplier's products are not listed.
Alexandra V. Vylegzhanina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... blots were incubated with primary antibodies against ORF1p overnight at 4°C with constant shaking: 1) anti-ORF1p IgG high-titers human serum samples in 1% non-fat dry milk (RPI) TBST in dilution 1:1,000-1000,000 ...
-
No products found
because this supplier's products are not listed.
Suzanne M. Castenmiller, et al.,
bioRxiv - Immunology 2024
Quote:
1-3×106 cells were cultured in 24-well plates at 37°C and 5% CO2 in 20/80 T-cell mixed media (Miltenyi) containing 5% human serum (HS) (Sanquin), 5% FBS ...
-
No products found
because this supplier's products are not listed.
Sonu Kumar, et al.,
bioRxiv - Microbiology 2020
Quote:
The SEC-purified Fab M4H2K1 and complex were each concentrated to 12 mg/ml before being screened at both 4 °C and 20 °C using our high-throughput CrystalMationTM robotic system (Rigaku) at TSRI (103) ...
-
No products found
because this supplier's products are not listed.
Emily K. Sims, et al.,
bioRxiv - Pathology 2019
Quote:
... Three of the donors with type 1 diabetes had detectable random serum C-peptide and 13 were classified by nPOD as C-peptide negative (random serum C-peptide <0.017nmol/L via TOSOH immunossay) (12) ...
-
No products found
because this supplier's products are not listed.
Huasheng Yu, et al.,
bioRxiv - Neuroscience 2019
Quote:
... cells were loaded at 37°C for 1 hr with 10 μg/ml Fluo-8 AM (AAT Bioquest, Inc.) supplemented with 0.01% Pluronic F-127 (w/v ...
-
No products found
because this supplier's products are not listed.
Erin A. Nekritz, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... we co-transfected the cells with 50 ng of pMIR-REPORT constructs containing the 3’UTR sequences together with 50 ng of a Renilla housekeeping control plasmid and 100 nM of miR-424 or miR-503 mimics or a miRNA negative control (Dharmacon #C-300717-05, #C-300841-05, or #CN-001000-01-05, respectively) using the TransIT-X2 reagent (Mirus 6003). 24 hours after transfection ...
-
No products found
because this supplier's products are not listed.
Saki Kiuchi, et al.,
bioRxiv - Biochemistry 2024
Quote:
RAW 264.7 cells were grown at 37 °C in DMEM (Dulbecco’s modified Eagle medium) supplemented with 10% EquaFETAL (Atlas Biologicals, Inc.) in a humidified 5% CO2 atmosphere ...
-
No products found
because this supplier's products are not listed.
Naoya Kase, et al.,
bioRxiv - Immunology 2020
Quote:
... or Human CXCL10 (IP-10) Kit (62HCX10PEG; Cisbio) was added to detect MCP-1 or IP-10 (Fig ...
-
8 Well Chambered Cover Glass with #1 cover glass (0.13-0.16mm), with lid, sterilized. Designed...
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Xueer Jiang, et al.,
bioRxiv - Cell Biology 2021
Quote:
All live cell imaging was performed with cells seeded in 35-mm glass-bottom dishes (P35G-1.5-10-C, MatTek Corp., Ashland, MA or D35C4-20-1.5-N, Cellvis, Mountain View, CA) mounted in a humidified chamber supplied with 5% CO2 inside a wrap-around environmental incubator (Okolab ...
-
No products found
because this supplier's products are not listed.
Olubu A. Adiji, et al.,
bioRxiv - Biochemistry 2023
Quote:
... maintained at 27°C in ESF 921 medium (Expression Systems). For transfection ...
-
No products found
because this supplier's products are not listed.
Katherine C. Woronowicz, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... 8°C) and Covaris microTUBE Snap-Cap tubes (Covaris 520045). Sequencing libraries were produced using the KAPA HyperPrep Kit (Roche 07137923001 ...
-
No products found
because this supplier's products are not listed.
Florent Ailloud, et al.,
bioRxiv - Microbiology 2023
Quote:
... Among the motifs not validated by PacBio data ...
-
No products found
because this supplier's products are not listed.
Pedro Espinosa, et al.,
bioRxiv - Neuroscience 2022
Quote:
... We incubated 48h at 4°C with rat anti-tdTomato (16D7, Kerafast) diluted 1:500 in a solution of 3% bovine albumin serum and 0.3% Triton X-100 prepared in PBS 1x ...
-
No products found
because this supplier's products are not listed.
Matthew Pierpoint, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Chromosomes were incubated in Tel C Cy 3 PNA probe (PNA Bio) at 83 °C for 5 minutes and allowed to hybridize at room temperature in a dark hybridization chamber for 2 hours at room temperature ...
-
No products found
because this supplier's products are not listed.
Monica Guerrero-Carrasco, et al.,
bioRxiv - Neuroscience 2024
Quote:
... overnight at 4°C: Chicken anti-GFP (1/500, Aves lab 1010); Rat anti-BrdU (1/500 ...
-
No products found
because this supplier's products are not listed.
Jun Xu, et al.,
bioRxiv - Zoology 2023
Quote:
... at 37°C for 30 min using glucose assay reagent (Megazyme; K-GLUC). We subtracted the amount of free glucose from the measurement and then normalized the subtracted values to protein levels in the supernatant ...
-
No products found
because this supplier's products are not listed.
Sylvie Deborde, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Experiments were performed at 37°C with a MFP-3D-BIO AFM microscope (Oxford Instruments). Fluorescent images of GFP-expressing HEI-286 SCs and RFP-expressing MiaPaCa-2 cancer cells were acquired together with the stiffness maps ...
-
No products found
because this supplier's products are not listed.
Molly Ohainle, et al.,
bioRxiv - Microbiology 2019
Quote:
... supplemented with 5% heat-inactivated human AB+ serum (Omega Scientific), 20 mM GlutaMAX-I ...