-
No products found
because this supplier's products are not listed.
Simon Gemble, et al.,
bioRxiv - Cell Biology 2022
Quote:
... + Triton X-100 (2000-C from Euromedex) 0,1% in PBS (20 min at 4°C) ...
-
No products found
because this supplier's products are not listed.
TM Tessi, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
-
No products found
because this supplier's products are not listed.
G Vilema-Enríquez, et al.,
bioRxiv - Neuroscience 2020
Quote:
The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
-
No products found
because this supplier's products are not listed.
Fu Guo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... observed using a Nikon C-C phase turret condenser (Nikon, Tokyo, Japan).
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No products found
because this supplier's products are not listed.
M. Beukema, et al.,
bioRxiv - Immunology 2023
Quote:
... female CB6F1 (C57Bl/6 x BALB/c F1) mice of 6-8 weeks (Envigo, Horst, the Netherlands) were acclimatized for 1.5 weeks ...
-
No products found
because this supplier's products are not listed.
Huibing Zhang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Luminance signal was measured using 0.5 s intervals after ligand addition (TECAN, 25°C). Concentration-responses were generated from the peak response ...
-
No products found
because this supplier's products are not listed.
Noah R. Johnson, et al.,
bioRxiv - Neuroscience 2021
Quote:
... recombinant human Aβ40 (rPeptide), recombinant human scrambled Aβ42 (rPeptide) ...
-
No products found
because this supplier's products are not listed.
Satadeepa Kal, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Recombinant Human Wnt 3a (BioVision) was administered at 150 ng/ml for 24 hours ...
-
No products found
because this supplier's products are not listed.
Pelin Kaya, et al.,
bioRxiv - Biochemistry 2023
Quote:
... after 10 μM coelenterazine 400a (#C-320, Gold Biotechnology) addition ...
-
No products found
because this supplier's products are not listed.
Chong Li, et al.,
bioRxiv - Genomics 2023
Quote:
... Hi-C libraries were generated with 1.5 M human cells as input using Proximo Hi-C kits v4.0 (Phase Genomics, Seattle, WA) according to the manufacturer’s protocol with the following change ...
-
No products found
because this supplier's products are not listed.
Anastasiia Lozovska, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Hybridization was performed at 65°C overnight in hybridization solution (50% formamide, 1.3 x SSC pH 5.5 [20 x SSC is 3M NaCl ...
-
No products found
because this supplier's products are not listed.
Lisa Maria Riedmayr, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
-
No products found
because this supplier's products are not listed.
Melanie Balbach, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... with a 10 x objective (mouse sperm) and a 20 x objective (human sperm) (UPLSAPO, NA 0.8; Olympus) was combined with a high-speed camera (ORCA Fusion ...
-
No products found
because this supplier's products are not listed.
Calvin Yeager, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The dialyzed 2C protein was centrifuged at 75,000 x g at 4 °C for 30 min to isolate soluble protein and concentrated using a Vivaspin Turbo 15 (Sartorius, 10 kDa MWCO) to 2 – 5 mL for preparative gel filtration ...
-
Chromatographically purified. A dialyzed, lyophilized pre-activated powder.
Cat# LS001643,
5x1 mg, $166.00
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M Fejzo, et al.,
bioRxiv - Genetics 2023
Quote:
... To digest the polypeptide 10 µL of Glu C enzyme (Worthington) at 100 µg/mL was added and the samples digested overnight at 37 °C ...
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No products found
because this supplier's products are not listed.
Josephine W. Thinwa, et al.,
bioRxiv - Microbiology 2022
Quote:
... guinea pig anti-p62/SQSTM1 (C-terminus) (Progen, GP62-C), mouse anti-SQSTM1 (Abnova ...
-
No products found
because this supplier's products are not listed.
Thomas Fischer, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... at 37°C in 5% CO2 on 10 cm dishes (Greiner Bioscience). For cell detachment Trypsin (Sigma Aldrich ...
-
No products found
because this supplier's products are not listed.
Alessandra Gallinaro, et al.,
bioRxiv - Immunology 2021
Quote:
... were coated with 2.5 µg/ml of mouse anti-human c-Myc 9E10 mAb (produced in house) or goat anti-human IgG (1:2000, Southern Biotech) for the standard ...
-
No products found
because this supplier's products are not listed.
James D. O’Leary, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and anti-c-Fos (Anti-c-Fos rabbit, 1:500, Synaptic System). Sections were then incubated in the appropriate AlexaFluor secondary antibody (AF488 for eYFP and AF596 for c-Fos ...
-
No products found
because this supplier's products are not listed.
Alexandro E. Trevino, et al.,
bioRxiv - Neuroscience 2021
Quote:
... PCW17 and PCW21 human cortical tissue was fixed overnight at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences). Samples were then washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
-
No products found
because this supplier's products are not listed.
Parishmita Sarma, et al.,
bioRxiv - Biochemistry 2022
Quote:
... cells were further incubated at 37°C for 10 min in the Flex Station 3 (Molecular Devices) before the assay was initiated ...
-
No products found
because this supplier's products are not listed.
Janna N. Hauser, et al.,
bioRxiv - Microbiology 2022
Quote:
... and growth was measured at OD600 in 30 min intervals at either 37°C or 42°C in an Epoch2 plate reader (BioTek).
-
No products found
because this supplier's products are not listed.
Matthew R. Rice, et al.,
bioRxiv - Biochemistry 2023
Quote:
... or recombinant human IKKβ protein (Signalchem, I03-10BG-10) in kinase reaction buffer in 96-well plates ...
-
No products found
because this supplier's products are not listed.
David W. Hammers, et al.,
bioRxiv - Cell Biology 2021
Quote:
... plasmid was generated by adding the C-terminal 20 amino acids of human H-Ras (NCBI Accession No. NP_005334) to a tagRFP-C vector (Evrogen) modified with a S158T point mutation to enhance photostability (Shaner et al. ...
-
No products found
because this supplier's products are not listed.
Liyen Loh, et al.,
bioRxiv - Immunology 2023
Quote:
... for 10 min at 110°C (NxGen Decloaker, Biocare) with a 10 min ambient cool down ...
-
No products found
because this supplier's products are not listed.
Kevin B. Weyant, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... A 27-amino acid B16-M30 peptide with N-terminal biotin and C-terminal polyhistidine (6xHis) motif for antibody-based detection was synthesized by Biomatik to ~85% purity ...
-
No products found
because this supplier's products are not listed.
Darrell Pilling, et al.,
bioRxiv - Immunology 2022
Quote:
... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Victoria L. Castro, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... C-terminal HCFC1 (Bethyl labs Cat # 50- 156-0286 purchased through Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Yongsung Kim, et al.,
bioRxiv - Genomics 2024
Quote:
37 °C Incubator (New Brunswick™ Galaxy™ 170S ...
-
No products found
because this supplier's products are not listed.
Linh Le, et al.,
bioRxiv - Cell Biology 2020
Quote:
... All cells were cultured at 37°C and 10% CO2 in RPMI 1640 medium supplemented with 10% FBS (Atlanta Biologicals) and 200 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) ...
-
No products found
because this supplier's products are not listed.
Quentin S. Fischer, et al.,
bioRxiv - Neuroscience 2023
Quote:
... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
-
No products found
because this supplier's products are not listed.
Dylan Stavish, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and signal captured on either x-ray film or digital detection using the LI-COR C-DiGit (LI-COR Biosciences).
-
No products found
because this supplier's products are not listed.
Fatima Amanat, et al.,
bioRxiv - Microbiology 2022
Quote:
... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
-
No products found
because this supplier's products are not listed.
Sangwoo Kim, et al.,
bioRxiv - Biophysics 2020
Quote:
... for a dorsal view of the tailbud and imaged at 25°C using a 40x water immersion objective (LD C-Apochromat 1.1 W, Carl Zeiss) on an inverted Zeiss Laser Scanning Confocal (LSM 710 ...
-
No products found
because this supplier's products are not listed.
Chanchal Chauhan, et al.,
bioRxiv - Cell Biology 2022
Quote:
... recombinant human TNFα (rHuTNF, #50435.50, Biomol, 10⍰ng/ml), Birinapant/Smac Mimetics (HY-16591 ...
-
No products found
because this supplier's products are not listed.
V D’Arienzo, et al.,
bioRxiv - Microbiology 2020
Quote:
... cDNA synthesis was performed with 0.25-1 µg of RNA in a 20 µL total reaction volume using a random hexamer/oligo dT strand synthesis kit as per the manufacturer’s instructions (10 min at 25°C; 15 min at 42°C; 15 min at 48°C; SensiFast, Bioline). All oligonucleotide sequences are listed in Table 1.
-
No products found
because this supplier's products are not listed.
Paulina Duhita Anindita, et al.,
bioRxiv - Biochemistry 2022
Quote:
... The protein in buffer C (2.2 mg/ml) was crystallized using Ligand-Friendly Screening HT-96 (Molecular Dimensions) for the initial screening and protein crystals grew in 0.1 M Bis-Tris Propane pH 6.5 ...
-
No products found
because this supplier's products are not listed.
Nannan Guo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... blocked in PBS containing 0.3% Triton X-100 and 10% normal donkey serum and incubated with primary antibody overnight at 4 °C overnight (Rockland, rabbit anti RFP ...
-
No products found
because this supplier's products are not listed.
Shan-Shan Lin, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 10 μM purified rabbit skeletal muscle actin (#AKL99-C, Cytoskeleton) was diluted in general actin buffer (5 mM Tris pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Jianing Song, et al.,
bioRxiv - Biochemistry 2020
Quote:
... recombinant human UBE1 (E-305, Boston Biochem), recombinant human UbcH5a/UBE2D1 (E2-616 ...
-
No products found
because this supplier's products are not listed.
Seunghee Oh, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Recombinant Snf1 kinase domain (Snf1-KD) was phosphorylated by recombinant human CaMKK2 (Abnova) for its activation before kinase assays (Figure 5F) ...
-
No products found
because this supplier's products are not listed.
Christian F. Deschepper,
bioRxiv - Genomics 2020
Quote:
... raised against recombinant human DDX3x (Genetex, Irvine, CA); polyclonal anti GAPDH (Proteintech ...
-
No products found
because this supplier's products are not listed.
Robert Düster, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and the thermal stability was monitored from 20°C to 90°C at a heating rate of 1.5°C/min using the PR.ThermControl software (NanoTemper).
-
No products found
because this supplier's products are not listed.
Wei-Sheng Sun, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 4°C and incubated at 4 °C with Ni-NTA (Macherey-Nagel). The Ni-NTA column was washed with 10 column volumes of 20 mM HEPES/NaOH (pH 7.0) ...
-
No products found
because this supplier's products are not listed.
Xuan Liang, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... the cells were incubated with 10 µg/mL mitomycin C (J63193, Alfa Aesar) in culture media at 37 □ for two hours ...
-
No products found
because this supplier's products are not listed.
Michael J Abrams, et al.,
bioRxiv - Evolutionary Biology 2020
Quote:
... and 0.1 mg/mL insulin (human recombinant, MP Biomedicals 0219390080). To introduce the molecular factors ...
-
No products found
because this supplier's products are not listed.
Domokos I. Lauko, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and incubated overnight at 28°C in Grace’s with 10% FBS (Gemini Bio-Products). Cells were transfected with 5 μg of pACT-GFP-actin using TransIT-Insect transfection reagent (Mirus Bio ...
-
Cat# HY-126297-25 mg,
25 mg, USD $1100.0
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Janelle Shari Weir, et al.,
bioRxiv - Neuroscience 2023
Quote:
... the novel synthetic ligand DCZ (MedChemExpress) was used to activate the DREADDs receptors (Nagai et al. ...
-
No products found
because this supplier's products are not listed.
Mireille M.J.P.E. Sthijns, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The absorbance was measured for 10 minutes at 37°C on a microplate reader (CLARIOstar, BMG LABTECH) at 412 nm and the slope of the curve was calculated ...
-
No products found
because this supplier's products are not listed.
Lyan van der Sleen, et al.,
bioRxiv - Biophysics 2023
Quote:
... a solution of 500 μM glycine betaine in buffer C was added in steps of 2 or 10 μL using a Hamilton syringe pump (Harvard apparatus). Excitation and emission wavelengths of 295 and 360 nm (and slit widths of 1 and 5 nm) ...