-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Matheus F. Sathler, et al.,
bioRxiv - Neuroscience 2022
Quote:
... NIH: HIV-1 IIIB gp120 Recombinant Protein from ImmunoDX, LLC ...
-
No products found
because this supplier's products are not listed.
Ching-Chieh Chou, et al.,
bioRxiv - Cell Biology 2023
Quote:
... and anti-Beta tubulin III (Tuj1) (1:500; Neuromics, CH23005 & 1:1000; Biolegend, 801201). The tNeurons were washed with PBS three times and incubated with fluorophore-conjugated secondary antibody solution in the dark for 1 hr at room temperature ...
-
No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
-
No products found
because this supplier's products are not listed.
Paul G Donlin-Asp, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Beta actin and Psd95 (Stellaris, Biosearch technology) were diluted into 100ul hybridization buffer and incubated with cells for 4 hours at 37°C ...
-
No products found
because this supplier's products are not listed.
Nanami Sakata, et al.,
bioRxiv - Plant Biology 2022
Quote:
... L-His (Tokyo Chemical Industry), and L-Lys (Nacalai tesque ...
-
No products found
because this supplier's products are not listed.
Charles A Berdan, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
-
No products found
because this supplier's products are not listed.
Clàudia Salat-Canela, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Recombinant proteins were checked and quantified in a polyacrylamide gel stained with Comassie Brilliant Blue (Amresco, 0472). The proteins were stored at −80°C ...
-
No products found
because this supplier's products are not listed.
KM Hudock, et al.,
bioRxiv - Immunology 2022
Quote:
... 100mU/ml human NE protein (Athens Research & Technology #16-14-051200, ART), 100μU/ml human PR3 protein (ART #16-14-161820 ...
-
No products found
because this supplier's products are not listed.
Madeleine F. Jennewein, et al.,
bioRxiv - Immunology 2023
Quote:
... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
-
No products found
because this supplier's products are not listed.
Su-Hyeong Kim, et al.,
bioRxiv - Cancer Biology 2020
Quote:
Expression of FoxQ1 protein in tissue microarrays of human luminal-type breast cancers (US Biomax, Rockville, MD; catalog # BR1508) and normal human mammary tissues (US Biomax ...
-
No products found
because this supplier's products are not listed.
Bethan Lloyd-Lewis, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Reporter expression and β-catenin stabilisation was induced in N1CreERT2 or SMACreERT2 females by a single intraperitoneal injection of tamoxifen free base (Euromedex) prepared in sunflower oil containing 10% ethanol (0.1 mg per g of mouse body weight) ...
-
No products found
because this supplier's products are not listed.
Emily P. Hurley, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Adeno-associated virus was injected using a Neuros 7002 Hamilton syringe coupled to an infusion pump (Pump 11 Elite Nanomite; Harvard Apparatus), which allowed a constant infusion rate of 2 nl/s ...
-
No products found
because this supplier's products are not listed.
Yeongjoon Lee, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 15N-labeled SARS-CoV-2 nsp7 sample was expressed and purified by the same methods as previously described in our NMR assignment paper.20 The recombinant Escherichia coli Rossetta2 pLysS was first grown in a M9 minimal medium containing 1 g/L 15N4HCl (Cambridge Isotope Laboratories), 4 g/L 13C6-D-Glucose (Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Diego Gilioli, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... 10% Human Serum (cat#ECS0219D from Euroclone) and IL-2 (cat#F027131010 from Novartis ...
-
No products found
because this supplier's products are not listed.
Michael Sporny, et al.,
bioRxiv - Biochemistry 2020
Quote:
Cryo-EM grids were prepared by applying 3 μl protein samples to glow-discharged (PELCO easiGlow™ Ted Pella Inc., at 15 mA for 1 minute) holey carbon grids (Quantifoil R 1.2/1.3 ...
-
No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
-
No products found
because this supplier's products are not listed.
Bhaskar Saha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... All GST-tagged proteins were expressed in Escherichia coli SoluBL21 (Genlantis). GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01) ...
-
No products found
because this supplier's products are not listed.
Hongyan Sun, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... The full length Spβ-catenin was synthesized (GENEWIZ, NJ) and fused with mCherry in the pCS2+ vector ...
-
No products found
because this supplier's products are not listed.
Kevin R. Trabbic, et al.,
bioRxiv - Immunology 2021
Quote:
β-1,3(1-6) glucan (Yeast Beta Glucan, Megazyme, Bray, Ireland; 3 mg) was suspended in 4.9 mL MilliQ H2O contaning 100 μL of 1 M NaOH ...
-
No products found
because this supplier's products are not listed.
Tomofumi Nakamura, et al.,
bioRxiv - Microbiology 2019
Quote:
Recombinant INHis proteins were examined with a TSKgel-SWXLG3000 column (TOSOH cor., Japan) at 1.0 ml/min in running buffer (20 mM HEPES pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Matteo Gentili, et al.,
bioRxiv - Immunology 2022
Quote:
... Beads were then washed 5 times with Ubiquitin IP buffer and proteins were eluted in Laemmli SDS Sample Buffer 1X with beta-mercaptoethanol (Boston Bioproducts) by boiling at 95°C for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Peter Hanna, et al.,
bioRxiv - Neuroscience 2020
Quote:
... A quadripolar His catheter (Abbott) was placed via the right external jugular vein and advanced until a His signal was visualized while a quadripolar catheter was inserted via the right femoral vein and advanced to the right atrium ...
-
No products found
because this supplier's products are not listed.
Reuben A. Saunders, et al.,
bioRxiv - Biophysics 2020
Quote:
... our ssrA-tagged titin protein was reacted at room temperature with three equivalents of ATTO 575Q maleimide (ATTO-TEC; GMBH) for 30 min in buffer A supplemented with 5 mM Tris (pH 8.0) ...
-
E-Cadherin protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five...
Cat# 5085-0.1MG,
0.1 mg, USD $235.0
Ask
Ines A Cadena, et al.,
bioRxiv - Bioengineering 2024
Quote:
... human (1 mg/mL, Advanced Biomatrix) and either GelMA (8.7% v/w ...
-
No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
-
No products found
because this supplier's products are not listed.
Wei-Hua Chiu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and adeno-associated virus (AAV) particles were injected with a Nanoject 3 (Drummond scientific) at a speed of 1 nl/sec for 30 secs injection with 30 secs pause for a total 33 cycles (i.e. ...
-
No products found
because this supplier's products are not listed.
Meryem T. Ok, et al.,
bioRxiv - Cell Biology 2022
Quote:
... FITC-associated fluorescence was measured by CLARIOstar Plus Microplate Reader (BMG Labtech, Ortenberg, Germany) after excitation at 483 nm and detection at 530 nm ...
-
No products found
because this supplier's products are not listed.
Christian Franke, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Immunostaining of specific receptors was performed by incubating cells overnight with AlexaFluor 647 conjugated primary antibodies (human CD4: OKT4, dilution: 1:100, source: Biolegend; human CD45: MEM-28, dilution: 1:2000, source: ExBio) diluted in Blocking solution ...
-
No products found
because this supplier's products are not listed.
Siran Zhu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 150 pmol of 6×His-streptavidin (ProteoGenix) was mixed with 5 μl of sieved beads (10% ...
-
No products found
because this supplier's products are not listed.
Dorothea Höpfner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... samples containing 100 ng recombinant protein were run over a 5 μm Jupiter C4 300Å LC column (Phenomenex, Torrance, California) using the 1260 Infinity LC system (Agilent Technologies ...
-
No products found
because this supplier's products are not listed.
Vaibhav Sidarala, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Anti-human TFAM (1:1000, PhosphoSolutions, Catalog# 1999-hTFAM), Anti-mouse TFAM (1:1000 ...
-
No products found
because this supplier's products are not listed.
C. Maresca, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 50 units of purified human PARP-1 (High Specific Activity hPARP-1, Trevigen) were incubated in a mixture containing 100 mM Tris-HCl pH 8 ...
-
No products found
because this supplier's products are not listed.
Luisa Santus, et al.,
bioRxiv - Genomics 2022
Quote:
... we removed duplicates from the samples tagged with UMIs (Zyagen) (Supplemental Table S1 ...
-
With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
Ask
Lukas Kunz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... for FLAG tagged effectors or WesternBright ECL HRP substrate (Advansta) for Pm8-HA.
-
No products found
because this supplier's products are not listed.
Xiao Ma, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... human BNP 1-32 (catalog#011-03, Phoenix Pharmaceuticals, Burlingame, CA), and human ANGII (catalog#A9525 ...
-
No products found
because this supplier's products are not listed.
Ling Ning Lam, et al.,
bioRxiv - Microbiology 2021
Quote:
... and inoculated at a ratio of 1:1000 into pooled human serum or pooled human urine (both purchased from Lee Biosolutions). At selected time points ...
-
No products found
because this supplier's products are not listed.
William S. Lawrence, et al.,
bioRxiv - Microbiology 2023
Quote:
... Biotinylated recombinant PA83 (List Biological Labs, Campbell, CA) was bound to streptavidin-coated plates (MesoScale Discovery ...
-
No products found
because this supplier's products are not listed.
Ole A.W. Haabeth, et al.,
bioRxiv - Immunology 2021
Quote:
... 2 μg of the RBD-his mRNA or GFP mRNA (Trilink) was formulated in 6.6 μl of PBS pH 5.5 with 1.37 μl of 5mM CART and added to the cells ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
-
No products found
because this supplier's products are not listed.
Jialiang S. Wang, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Samples (60 µl well−1) were then added along with a standard curve of murine recombinant Sclerostin (Alpco, Salem, NH) and plates were incubated overnight at 4 °C ...
-
No products found
because this supplier's products are not listed.
Eike K. Mahlandt, et al.,
bioRxiv - Cell Biology 2021
Quote:
... BOECs were stimulated with 1 U/ml human α-thrombin (HCT-0020, Haematologic technologies) diluted in phosphate-buffered saline.
-
No products found
because this supplier's products are not listed.
Surbhi Verma, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human MDMs (hMDMs) were isolated from human PBMCs and separated from healthy human donors’ blood using PolymorphPrep (PROGEN,1895) layering and centrifugation ...
-
No products found
because this supplier's products are not listed.
Miryam Adelfio, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Gingival cells were maintained in culture up to passage 6 in appropriate medium supplemented with associated growth factor kits (Lifeline Cell Technology, Frederick, MD). For passaging cells ...
-
No products found
because this supplier's products are not listed.
Firyal Ramzan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkyne-tagged fatty acid analogs (alkyne-myristate (13-tetradecylnoic acid; 13-TDYA; Click Chemistry Tools 1164) or alkyne-palmitate (15-hexadecynoic acid ...
-
No products found
because this supplier's products are not listed.
Shinichiro Komaki, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein samples were detected with 1:1000 diluted anti-RFP (AB233; Evrogen) for BORR:RFP detection and 1:2000 diluted anti-Histone H3 (ab1791 ...
-
No products found
because this supplier's products are not listed.
Daniel Schindler, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... The DNA Hi-C libraries were sheared into 300 bp using a Covaris S220 apparatus (Covaris) and the biotin-labeled fragments were selectively captured by Dynabeads Myone Streptavidin C1 ...
-
RocketFuel™ activates 500mL Cell Systems SF-4Z0-500-R. RocketFuel™ is the broad-spectrum mitogen...
Cat# SF-4ZR-500-R,
8.0 mL, $95.0
Ask
Changsheng Chen, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Human umbilical vein endothelial cells (HUVECs, Cell System) were cultured in endothelial cell growth medium according to the protocol provided by the manufacturer (VascuLife ...
-
No products found
because this supplier's products are not listed.
Katharina Hohenwallner, et al.,
bioRxiv - Biochemistry 2022
Quote:
... 2.5% human platelet lysate, and 1 IU/ml heparin (both PL BioScience, Aachen, Germany) on standard T-flasks (Sarstedt, Nümbrecht, Germany), in a humidified incubator at 37°C and 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Linyi Zhu, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Human trapezial cartilage was ground to a powder using Cryo-Cup Grinder (Biospec, Bartlesville) and then RNA was extracted using Qiagen RNeasy Micro Kit.