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No products found
because this supplier's products are not listed.
Sonja Giger, et al.,
bioRxiv - Bioengineering 2021
Quote:
... recombinant human epidermal growth factor (hEGF, 20 ng/ml, Chimerigen Laboratories, CHI-HF-210EGF), recombinant human platelet derived growth factor (hPDGF-AB ...
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No products found
because this supplier's products are not listed.
Maria Körner, et al.,
bioRxiv - Cell Biology 2023
Quote:
Pulldown assays using immobilized GST fusion proteins or biotinylated peptide (Biotin- CQGLYFHINQTLREAHFHSLQHRG-COOH; PANATecs GmbH, Tübingen, Germany) were essentially performed as described (Böhm et al ...
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No products found
because this supplier's products are not listed.
Jennyfer M. Mitchell, Scott A. Nichols,
bioRxiv - Evolutionary Biology 2019
Quote:
... His-EmFAK and GST-EmITGB1 (Syd Labs) recombinant proteins ...
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No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
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No products found
because this supplier's products are not listed.
Md. Abu Sayeed, et al.,
bioRxiv - Immunology 2022
Quote:
The purified recombinant ICP1 bacteriophage proteins were used to raise mAbs via a commercial vendor (ProMab Biotechnologies, Inc.) using a conventional hybridoma technique (28) ...
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... Purified recombinant viral proteins were solubilized in PBS and biotinylated chemically with sulfo-NHS-LC-biotin (sulfo-NHS-LC-biotin, Proteochem, USA) at a molar ratio of 1:1 ...
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No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
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No products found
because this supplier's products are not listed.
Delphine Planas, et al.,
bioRxiv - Immunology 2021
Quote:
... 2) a CE-Marked ELISA assay for detection of IgG against the full-length recombinant N protein from Epitope Diagnostics (EDITM Novel coronavirus COVID-19 IgG). The test has a specificity of 96% and a sensitivity of 81% after 28 days POS in our hands ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Mazen Makke, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 250µg recombinant GST-CpxII-CTD or its mutant variants were incubated with 100µL GST bead slurry for 1 hour at room temperature while shaking at 1000 rpm (Vibrax, IKA, Staufen, Germany). Beads were then centrifuged (200 rpm ...
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No products found
because this supplier's products are not listed.
Ana P. Peredo, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Primary human AF cells obtained from healthy human tissue were purchased from Articular Engineering. AF cell lines AF26 (30-year-old donor) ...
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No products found
because this supplier's products are not listed.
Tracy J. Berg, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Primary human astrocytes (3H Biomedical) were cultured in Astrocyte Medium (3H Biomedical ...
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No products found
because this supplier's products are not listed.
Xinyu Xie, et al.,
bioRxiv - Immunology 2024
Quote:
... media containing PBS or recombinant IL-1β (10 ng/ml) (Abbkine, PRP100051, USA) was added to the basal chambers for 3 days.
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No products found
because this supplier's products are not listed.
Goncalo Fernandes, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Injections and recombinant selection based on DsRed expression in the eye were performed by BestGene. For transformants ...
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No products found
because this supplier's products are not listed.
Helen R. McPherson, et al.,
bioRxiv - Physiology 2021
Quote:
... Human FXIII-A2B2 from Zedira (Darmstadt, Germany) was further purified from contaminating albumin and glucose by Hiload 16/60 superdex 200 (GE Healthcare ...
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No products found
because this supplier's products are not listed.
Antje Neeb, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... panBAG-1 (human, rabbit monoclonal, RM356, RevMAb), panBAG-1 (mouse ...
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No products found
because this supplier's products are not listed.
Justine Cristante, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... or pTer1 control vector (pTer1 cells)16 were authenticated by CellCheck human 16 Plus Test (9 human STR marker profile + 7 additional Human STR marker, Inter-species contamination Test and STAT-mycoplasma testing) (IDEXX Analytics). They were grown in Dulbecco’s Modified Eagle Medium:F-12 (DMEM/F-12 Glutamax ...
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No products found
because this supplier's products are not listed.
M Azharuddin, et al.,
bioRxiv - Immunology 2021
Quote:
... or anti-human IgA-HRP (Nordic BioSite, Täby, Sweden) was added to separate wells with diluted serum samples and incubated 90 min ...
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No products found
because this supplier's products are not listed.
Astrid Hendriks, et al.,
bioRxiv - Microbiology 2023
Quote:
... Human neutrophils were freshly isolated using Polymorphprep (Alere technologies) per manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Jorge Gomez-Deza, et al.,
bioRxiv - Neuroscience 2023
Quote:
The Human Genome-Wide CRISPRi Dual-sgRNA Library (Cellecta KIDHGW-105K-P ...
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MCAT (180 aa) recombinant protein +GST-tag at NT.
Cat# HRP1007,
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Jared M. Fischer, et al.,
bioRxiv - Bioengineering 2024
Quote:
... A375-Luc/iRFP (human melanoma, Creative Biogene CSC-RR0254), MCF7 (human breast cancer ...
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No products found
because this supplier's products are not listed.
Neetu Singh Kushwah, et al.,
bioRxiv - Molecular Biology 2021
Quote:
AAE3 protein sequences from Medicago truncatula (GenBank ...
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No products found
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Human PRG-1 obtained from DNASU Plasmid Repository (The Biodesign Institute/Arizona State University ...
-
No products found
because this supplier's products are not listed.
Padmapriya Sekar, et al.,
bioRxiv - Immunology 2023
Quote:
... 330 μg/mL iron-saturated human holo-transferrin (BBI solutions), 10 μg/mL recombinant human insulin (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Helene Jahn, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Protein concentration was determined by SDS-PAGE (Coomassie or Fluorescence Protein gel stain (Lamda Biotech)) in comparison with standards ...
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No products found
because this supplier's products are not listed.
Soumya Mukherjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Commercial pooled human plasma was purchased from Affinity Biologicals (Ancaster, ON). Purified human neutrophils ...
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No products found
because this supplier's products are not listed.
Raeann Goering, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... with protein ladder (Gel Company FPL-008). Gels were imaged with a Sapphire molecular imager (Azure Biosystems ...
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No products found
because this supplier's products are not listed.
Moon Kee Meang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Protein bands were visualized using ECL reagent (Abfrontier, Korea) and Uvitec HD9 (Uvitec ...
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No products found
because this supplier's products are not listed.
Pehuén Pereyra Gerber, et al.,
bioRxiv - Microbiology 2021
Quote:
... incubated for 30 min with a PE-labeled anti–human IgG-Fc antibody (Leinco/Biotrend), washed again ...
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No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
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No products found
because this supplier's products are not listed.
Xian Zhou, et al.,
bioRxiv - Immunology 2020
Quote:
... Human B cell proliferation was measured by 3[H]-thymidine incorporation (1 μCi/mL) (American Radiolabeled Chemicals).
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No products found
because this supplier's products are not listed.
Shivani Ahuja, et al.,
bioRxiv - Biochemistry 2020
Quote:
... the protein was mixed 2:3 with monoolein (Nu-Chek Prep) and then 70 nl of this material was deposited on a glass slide (Molecular Dimensions) ...
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No products found
because this supplier's products are not listed.
Adam Beck, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The antibodies and protein standards are commercially available from EnCor Biotechnology Inc.
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No products found
because this supplier's products are not listed.
Arthur Forer, Shotaro Otsuka,
bioRxiv - Cell Biology 2023
Quote:
Gold beads (15 nm) conjugated with Protein A (Cytodiagnostics, Burlington, Ontario, Canada) were absorbed on both sides of the sections as fiducial markers for tomography reconstruction ...
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No products found
because this supplier's products are not listed.
Mariah Beaver, et al.,
bioRxiv - Genomics 2021
Quote:
Chromatin was extracted and sheared from ~120 mg human hippocampus using truChIP Chromatin Shearing Kit (Covaris Inc., MA, USA) following the manufacturer’s instructions ...
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Fluorescent Method
Cat# FPRO200,
FluoProdige fluorescent Dye (10ml) + Dilution Buffer (100ml) + BSA(500µg) lyoph, USD $229.00/KIT
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Andrew S. Flies, et al.,
bioRxiv - Immunology 2020
Quote:
... Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010) to a final concentration of 0.1 μg/μL and was mixed using interlocked syringes to form an emulsion ...
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No products found
because this supplier's products are not listed.
Lennart Schada von Borzyskowski, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... all protein digests were desalted using C18 microspin columns (Harvard Apparatus, Holliston, USA) according to the manufacturer’s instructions.
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No products found
because this supplier's products are not listed.
Marijn Knip, Emy Latul, Frank LW Takken,
bioRxiv - Plant Biology 2023
Quote:
Proteins were isolated from six leaf discs (5mm Ø World Precision Instruments (WPI)) ...
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No products found
because this supplier's products are not listed.
Blasi Maria, et al.,
bioRxiv - Immunology 2020
Quote:
... Plates were then incubated with 2 μg/ml biotinylated rabbit anti-human IFN-γ (U-CyTech biosciences, Utrecht, The Netherlands) for 2 hours at room temperature ...
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No products found
because this supplier's products are not listed.
Kelly M. Hennessey, et al.,
bioRxiv - Cell Biology 2020
Quote:
... the C terminus of GL50803_8445 was tagged with an 11-amino acid flexible linker and the fluorescent protein mNeonGreen (Allele Biotechnology). The mNG-N11-Neo vector was constructed for this purpose by amplifying mNeonGreen using the primers listed in Table S1 ...
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No products found
because this supplier's products are not listed.
Julio Aleman, et al.,
bioRxiv - Bioengineering 2021
Quote:
... The secreted levels of alpha-GST by the organoids were quantified using a GST alpha assay kit (GS41, Oxford Biomedical Research; Rochester Hills, MI). Absorbance was read on a Spectramax M5 plate reader (Molecular Devices ...
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No products found
because this supplier's products are not listed.
Yan Wang, Meiling Lian, Liping Song, Shengzhou Wu,
bioRxiv - Neuroscience 2020
Quote:
... fractions using commercially available kits (Cat#CSB-E08299h for human Aβ40; Cat#CSB-E10684h for human Aβ42, CUSABIO TECHNOLOGY LLC, Wuhan, China) per the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Vaibhav Sidarala, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Anti-human TFAM (1:1000, PhosphoSolutions, Catalog# 1999-hTFAM), Anti-mouse TFAM (1:1000 ...
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No products found
because this supplier's products are not listed.
Amy L. Kimble, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Frozen mouse and human brain tissues were mechanically homogenized (Next Advance Bullet Blender BB724M with 3.2 mm stainless steel beads using setting 4 for 4min at 4C) ...
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No products found
because this supplier's products are not listed.
Duilio M. Potenza, et al.,
bioRxiv - Physiology 2024
Quote:
... and human cardiac fibroblasts was extracted with Trizol Reagent (TR-118, Molecular Research Center) following the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
MaryAnn Martin, Irene L.G. Newton,
bioRxiv - Microbiology 2023
Quote:
Proteins were separated on 4-20% Tris-Glycine NB precast minigels (NuSep) and transferred to PVDF membrane in Tris-Glycine transfer buffer with 15% methanol at 40v on ice for 3 hours ...
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No products found
because this supplier's products are not listed.
Gabriela O. Bodea, et al.,
bioRxiv - Genomics 2023
Quote:
... MeCP2: We quantified MeCP2 protein expression using Imaris 9.5.1 (Bitplane, Oxford Instruments and we analyzed two hippocampal sections per animal ...
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No products found
because this supplier's products are not listed.
Paweł P. Knejski, et al.,
bioRxiv - Biochemistry 2023
Quote:
... purified proteins were diluted to 0.05 mg/mL and applied onto plasma-cleaned (Gatan Solarus) copper grids with a continuous carbon layer (Electron Microscopy Sciences) ...
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No products found
because this supplier's products are not listed.
Hagit Hak, et al.,
bioRxiv - Plant Biology 2024
Quote:
... and images of protein bands were acquired and quantified using the Alliance Q9 software (UVITEC).