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Recombinant Human CD3e Molecule Epsilon CD3 TCR Complex His tagged
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Creative Biomart Sponsored
Recombinant Human CD3E (NP_000724.1) precursor extracellular domain (Met 1-Asp 126), fused with...Cat# CD3E-2194H, 20.0 µg, USD $399.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of General Virology doi: 10.1099/jgv.0.001231Quote: ... His-tagged recombinant human CD63 protein was obtained from ThermoFisher Scientific Sino Biological (China).The expression and purification of the CD63 LEL domain were performed by the same method used for nsP1a and nsP1a/4 ... -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in eLife doi: 10.7554/eLife.47528Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Frontiers in Microbiology doi: 10.3389/fmicb.2019.02328Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
Peprotech
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019Quote: ... recombinant human FGF2 (Peprotech) was added at concentrations ranging from 5 ng/ml to 80 ng/ml ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... CD3+ T cells (mouse anti-human CD3, BD Biosciences), CD4+ T helper cells (mouse anti-human CD4 ... -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020, published in eLife doi: 10.7554/eLife.65365Quote: ... Recombinant His-tagged and biotinylated human ACE2 protein (Sino Biological, Cat # 10108-H08H-B) was immobilized on a Streptavidin (SA ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2018, published in eLife doi: 10.7554/elife.42274Quote: ... and 6xHis tagged recombinant human integrin heterodimers were purchased from R&D Systems. The plates were incubated with integrins in TBS buffer (50 mM Tris-Cl ... -
GenScript
No products found because this supplier's products are not listed.Cited in The C. difficile clnRAB operon initiates adaptations to the host environment in response to LL-37bioRxiv - Microbiology 2018, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007153Quote: Recombinant N-terminally His-tagged ClnR was produced by GenScript (Piscataway, NJ). Gene transcription in the clnR mutant complemented with His-tagged ClnR confirmed the functionality of this protein (Table S12) ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... and 6x-HIS-tagged-HOXB2 (Addgene 8522) were obtained from commercial vendors and cloned into pCMV6 vector and transfected into HEK cells ... -
abcam
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2019Quote: ... recombinant human PD-L1 protein and recombinant human tau protein were purchased from Abcam. HRP-conjugated goat anti-Mouse IgG (H+L ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2017, published in Nature Microbiology doi: 10.1038/s41564-018-0203-xQuote: ... Purification of the His-tagged/GST-tagged recombinant proteins was performed as described by the manufacturer (Clontech/GE Healthcare). -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Nature Chemical Biology doi: 10.1038/s41589-019-0304-8Quote: Recombinant Flag-tagged RNF114 was used as bait to precipitate pure recombinant p21 (Origene Technologies Inc. ... -
Stemcell Technologies
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020, published in Scientific Reports doi: 10.1038/s41598-020-71548-zQuote: ... Human T cells were also isolated from the thymus tissue in humanized SRG rats via immunomagnetic selection using anti-human CD3 antibody (EasySep™ Human CD3 Positive Selection, Catalog # 17951, Stemcell Technologies) and treated without (vehicle ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... and CD3e (UCHT1, Dako). Secondary antibodies goat anti-rat AlexaFluor® 594 (1:200 ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018, published in Nature Communications doi: 10.1038/s41467-018-03876-8Quote: ... recombinant human basic FGF (Promega), SB202190 (EMD Chemicals ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2018, published in Nature Communications doi: 10.1038/s41467-018-07251-5Quote: ... His-tagged proteins were immobilized on Ni-NTA (Merck Millipore), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01006-18Quote: Recombinant His-tagged proteins were resolved by AnykDTM Mini-Protean TGX gels (Bio-Rad) and transferred to nylon-supported nitrocellulose ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in iScience doi: 10.1016/j.isci.2019.100759Quote: ... human recombinant IL-2 (Roche) was also added fresh immediately prior to use at a final concentration of 100 IU/ml ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... To control for recombinant bacterial MCO expression anti-His-HRP (Miltenyi Biotec) was used (at a dilution of 1:10000 ... -
Cell Signaling Technology
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mBio doi: 10.1128/mBio.01905-18Quote: ... His-tagged proteins were detected with mouse anti-His tag (27E8) antibody at a dilution of 1:1000 (Cell Signaling, Danvers, MA) and an anti-mouse IgG HRP linked antibody at a dilution of 1:2000 (Cell Signaling ... -
Bio X Cell
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019Quote: ... anti-human CD3 (OKT3, Bio X cell, anti-human CD28 (9.3 Bio X Cell), anti-human CD45 (REA747 ... -
Enzo Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Nature Structural & Molecular Biology doi: 10.1038/s41594-020-0380-1Quote: ... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ... -
Boston Biochem
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2018Quote: Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 (40 mM Hepes/KOH pH 8.0 ... -
ImmunoTools
No products found because this supplier's products are not listed.Cited in Primary human macrophages are polarized towards pro-inflammatory phenotypes in alginate hydrogelsbioRxiv - Immunology 2019Quote: ... Recombinant human M-CSF was obtained from ImmunoTools. Antibodies and matched isotype controls were obtained from Miltenyi. -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Immunology 2017Quote: High-throughput sequencing of TCRs was performed using the ImmunoSEQ assay (Adaptive Biotechnologies) with TCR-β and/or TCR-α/δ assays for each sample using a multiplex PCR approach following by Illumina high-throughput sequencing (52). -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020, published in Journal of Comparative Neurology doi: 10.1002/cne.25103Quote: ... the sections were washed and incubated with the avidin-biotin complex (AB Complex, Vector Laboratories Ltd.) for 1 hour at room temperature ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.Cited in Allosteric deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plantsbioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Immunology 2018, published in Mucosal Immunology doi: 10.1038/s41385-018-0049-9Quote: ... goat anti-human CD3 was from Santa Cruz; rabbit anti-human CD8 was from Abcam ... -
Beckman
No products found because this supplier's products are not listed.Cited in Transgene-free hematopoietic stem and progenitor cells from human induced pluripotent stem cellsbioRxiv - Cell Biology 2017Quote: ... TCR αβ clone IP26A and TCR γ δ clone IMMU510 (all from Beckman Coulter antibodies, Brea, USA). -
Corning
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019Quote: ... 40ng/ml human recombinant basic fibroblast growth factor (bFGF, Corning), 1xB27 (Gibco) ... -
Proteintech
No products found because this supplier's products are not listed.Cited in PHAROH lncRNA regulates c-Myc translation in hepatocellular carcinoma via sequestering TIARbioRxiv - Cancer Biology 2021Quote: ... Recombinant human TIAR (Proteintech) was added at indicated concentrations to the probe (∼2 fmol ... -
Tonbo Biosciences
No products found because this supplier's products are not listed.bioRxiv - Immunology 2017, published in The Journal of Experimental Medicine doi: 10.1084/jem.20172018Quote: Splenocytes and lymphocytes obtained from conditional KO and WT mice were stimulated in complete RPMI with 3 µg/ml biotinylated anti-CD3 and anti-CD28 in the presence of 15 µg/ml streptavidin (anti-mouse CD3e biotin, clone 145-2C11, Tonbo Biosciences #30-0031-U500; ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Immunology 2019, published in Frontiers in Immunology doi: 10.3389/fimmu.2019.02706Quote: ... OT-II TCR transgenic (Strain 004194) and OT-I TCR transgenic mice (Strain 003831) were acquired from Jackson Labs. We acquired C57BL/6N embryonic stem (ES ... -
SignalChem
No products found because this supplier's products are not listed.Cited in Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 proteinbioRxiv - Neuroscience 2017, published in eLife doi: 10.7554/eLife.24779Quote: ... Immunoprecipitated myc-PER2 were incubated with GST-tagged recombinant human SIK3 (aa.1-307) (SignalChem) in kinase assay buffer (50 mM Tris-HCl ... -
Lonza
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2018, published in PLOS ONE doi: 10.1371/journal.pone.0208287Quote: ... recombinant human epidermal growth factor (EGF) (Lonza, CC-4017). Metabolic assay reagents ... -
Biovision
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... transforming growth factor beta2 (TGFβ2) 1ng/ml (human-recombinant, Biovision) and fibroblastic growth factor FGF2 5ng/ml (human-recombinant ... -
NanoTemper
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in PLOS Pathogens doi: 10.1371/journal.ppat.1007848Quote: Three independent MST experiments were performed with His-tagged BspC labelled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies) according to manufacturer’s instructions ... -
Cytoskeleton
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ... -
Jackson ImmunoResearch
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: ... ChromPure Mouse IgG whole molecule (Jackson ImmunoResearch, West Grove, PA), for 30 minutes on ice ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Biophysics 2019, published in Nature Communications doi: 10.1038/s41467-019-12415-yQuote: Single molecule FRET experiments were performed with an inverted microscope (1X73, Olympus) with prism-based total internal reflection ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... with SensiMix SYBR Hi-ROX Kit (Bioline) following manufactures instructions ... -
GeneCopoeia
No products found because this supplier's products are not listed.Cited in Quantitative proteomics identifies novel PIAS1 substrates involved in cell migration and motilitybioRxiv - Cancer Biology 2019Quote: The bacterial expression vectors pReceiver-B11 for His-NSMCE2 and His-PFDN2 recombinant protein expression were purchased from Genecopoeia, Inc (Rockville ... -
Calbiochem
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019, published in Glia doi: 10.1002/glia.23792Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ... -
PromoCell
No products found because this supplier's products are not listed.bioRxiv - Genomics 2017, published in Scientific Reports doi: 10.1038/s41598-017-14892-xQuote: ... and 5 μg/ml recombinant human insulin (PromoCell, Cat: C-39275). We differentiated fully confluent cells for four weeks under serum starvation and treated cells with DMSO vehicle (Control ... -
PacBio
No products found because this supplier's products are not listed.Cited in Contiguous and accurate de novo assembly of metazoan genomes with modest long read coveragebioRxiv - Genomics 2016, published in Nucleic Acids Research doi: 10.1093/nar/gkw654Quote: ... As single molecule technologies (PacBio and Oxford Nanopore) do not require any sequence amplification step ... -
ChromoTek
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2019Quote: ... Tagged proteins were immunoprecipitated with Myc-Trap_MA beads (ChromoTek) or anti-FLAG M2 Affinity gel (Sigma ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... CD3 (clone LN10, NCL-L-CD3-565 (Leica Novocastra) – Opal 540) ...