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No products found
because this supplier's products are not listed.
Keiko Masuda, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
Non-labeled quantification tags were synthesized by Fluorenylmethyloxycarbonyl (Fmoc) chemistry using a peptide synthesizer SyroWave (Biotage, Uppsala, Sweden). Fmoc amino acids were purchased from Watanabe Chemical Industries and contained the following side chain protecting groups ...
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No products found
because this supplier's products are not listed.
Amanda L. Photenhauer, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Custom α-Sas6T antiserum was generated by rabbit immunization with purified recombinant Sas6T protein (Lampire Biological Laboratories). The resulting antiserum was used for western blotting and cell staining ...
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No products found
because this supplier's products are not listed.
Nozomi Ugajin, et al.,
bioRxiv - Molecular Biology 2023
Quote:
RNA samples were extracted from zebrafish embryos or FLAG-immunoprecipitants using TRI Reagent (Molecular Research Center). Ethachinmate (Nippon gene ...
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No products found
because this supplier's products are not listed.
Carlos J Nogueras-Ortiz, et al.,
bioRxiv - Neuroscience 2020
Quote:
... or anti-human tetraspanin CD81 (Ancell Corporation, Bayport, MN) biotinylated antibodies ...
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No products found
because this supplier's products are not listed.
Arjen J. Jakobi, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... For the gold labeling experiments the His6-tag was not removed to allow binding of 5 nm Ni-NTA-Nanogold® (Nanoprobes). For consistency the His6-tag was also kept on the proteins for the co-pelletation assay.
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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No products found
because this supplier's products are not listed.
Masaya Matsubayashi, et al.,
bioRxiv - Biochemistry 2019
Quote:
The human hepatocellular carcinoma cell HepG2 was purchased from Cellular Engineering Technologies ...
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No products found
because this supplier's products are not listed.
Kyle E. Landgraf, et al.,
bioRxiv - Cancer Biology 2019
Quote:
Human PBMC stimulation and immune-phenotyping studies were performed by iQ Biosciences. Briefly normal PBMCs from three donors were seeded in 96-well plates at 1×105 cells/well and exposed to a 10-fold dilution series of either U2S3-hFc-mutIL2 or U2S3-hFc-wtIL2 (wild-type IL2 ...
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No products found
because this supplier's products are not listed.
Aloysius Wong, et al.,
bioRxiv - Plant Biology 2019
Quote:
The UV-visible spectra of affinity purified recombinant DGK4 (200 µg/mL) was recorded on a PHERAstar FS micro-plate reader (BMG Labtech, USA). The heme environment of DGK4 was characterized by the addition of a reducing agent ...
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No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...
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No products found
because this supplier's products are not listed.
M. A. Rossotti, et al.,
bioRxiv - Bioengineering 2021
Quote:
... three-fold dilutions of VHH-Fcs were prepared in V- Bottom 96-well microtiter plates (Globe Scientific, Mahwah, NJ, Cat# 120130) and mixed with 50 µL of CHO-SPK cells ...
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No products found
because this supplier's products are not listed.
Rebecca O’Cleirigh, Roslyn Gibbs,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... containing 5% (v/v) FCS and incubated overnight in a humidified atmosphere at 37°C and 5% CO2 (Nuaire, DH Autoflow). After 24 hours 1.5mL of the media was removed and media with herb extract or media only control was added ...
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No products found
because this supplier's products are not listed.
Lindsey M. Brier, Joseph P. Culver,
bioRxiv - Neuroscience 2021
Quote:
... The N=16 mice used for FC matrix computation had stainless steel EEG self-tapping screws (BASI Inc., West Lafayette, IN, USA) fixed at approximately -1mm posterior to bregma ...
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No products found
because this supplier's products are not listed.
Sebastian Schlaweck, et al.,
bioRxiv - Immunology 2024
Quote:
Splenic T cells were isolated by CD3e MACS kit (Miltenyi #130-094-973) from mice injected with 0.2 µg α-GalCer (1 nmol; Axxora, #ALX-306-027) 24h before ...
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No products found
because this supplier's products are not listed.
Doris Krauter, et al.,
bioRxiv - Neuroscience 2021
Quote:
... using a diamond knife (Histo HI 4317, Diatome). Afterwards sections were stained according to Gallyas52 and with Methylene blue/ Azur II for 1 min ...
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No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Recombinant mouse TNFSF11 (IBI Scientific, Indiana), also known as RANKL ...
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No products found
because this supplier's products are not listed.
Chafen Lu, et al.,
bioRxiv - Microbiology 2019
Quote:
... Secondary antibodies were rabbit polyclonal anti-His (Delta Biolabs), Horseradish peroxidase (HRP)-anti-rabbit and HRP-anti-mouse IgG (GE Healthcare ...
-
No products found
because this supplier's products are not listed.
Lindsey R. Conroy, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Recombinant PNGaseF Prime was obtained from Bulldog Bio, Inc ...
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No products found
because this supplier's products are not listed.
Lorna Ewart, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Cryopreserved human Kupffer cells (Samsara Sciences) and human Stellate cells (IXCells) were thawed according to their respective vendor/Emulate protocols on the day of seeding ...
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No products found
because this supplier's products are not listed.
Camden R. Bair, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 8% heat-inactivated newborn calf serum (HI-NBCS, Rocky Mountain Biologicals), 1.5 g/ml Na2CO3 (Amresco) ...
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No products found
because this supplier's products are not listed.
Alireza Ghanbarpour, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... A synthetic degron-tag peptide consisting of maleimide-GSGSWSHPQFEKAANDENYALAA (21st Century Biochemicals, Inc.), where the underlined sequence is the ssrA tag ...
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Highly cross-adsorbed secondary antibody conjugate that reacts with both heavy and light chains....
Cat# R-05766-250,
0.250 ml, USD $79.00/ea
Ask
Lukas Kunz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... for FLAG tagged effectors or WesternBright ECL HRP substrate (Advansta) for Pm8-HA.
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No products found
because this supplier's products are not listed.
Kathleen M. Mulvaney, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... the N-terminal tag was removed by incubation with HRV 3C protease (AG Scientific) and further purified by Superose 6 column ...
-
No products found
because this supplier's products are not listed.
Katherine P. Mueller, et al.,
bioRxiv - Bioengineering 2021
Quote:
... Recombinant sNLS-SpCas9-sNLS Cas9 (Aldevron, 10 mg/ml, total 0.8 µl) was added to the complexed gRNA at a 1:1 molar ratio and incubated for 15 minutes at 37°C to form an RNP ...
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No products found
because this supplier's products are not listed.
Andrew R. McEwan, et al.,
bioRxiv - Genetics 2021
Quote:
... from human DNA (Cambio, UK) using the following primers ...
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No products found
because this supplier's products are not listed.
Jay Lubow, et al.,
bioRxiv - Immunology 2019
Quote:
... CAp24 concentrations were measured by comparison to recombinant CAp24 standards (ViroGen, Watertown, Massachusetts).
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No products found
because this supplier's products are not listed.
Maria Carmen Rodenas, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Thrombin generation assay-calibrated automated thrombogram (TAG-CAT) was conducted as previously described [24] (Diagnostica Stago). Samples were processed in duplicate ...
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No products found
because this supplier's products are not listed.
Lin Zhuang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Human colon normal epithelial cells (FHC) and CRC cell lines (HT29 and LoVo ...
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No products found
because this supplier's products are not listed.
Tsuyoshi Hattori, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The neurons were cultured with or without SPARCL1 recombinant protein (80 nM, ATGP3891, NKMAX) from day 8 to day 14 as previously done (34) ...
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No products found
because this supplier's products are not listed.
Robert LeDesma, et al.,
bioRxiv - Microbiology 2022
Quote:
... or H249A-HA-tag were seeded onto separate glass coverslips (#1.5; 10 mm; Thomas Scientific, Swedesboro, NJ) in a 24-well plate at 100,000 cells per well ...
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No products found
because this supplier's products are not listed.
Erin J. Vanzyl, et al.,
bioRxiv - Cell Biology 2020
Quote:
... ATF3 mRNA expression was determined using Bioline SensiFAST™ Probe HI-ROX Master Mix (FroggaBio Inc, Toronto, ON) with SYBR green ...
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No products found
because this supplier's products are not listed.
Siyi Gu, et al.,
bioRxiv - Cell Biology 2023
Quote:
HEK293 cells expressing Flag-SNAP-CCR5 were seeded onto 10-mm glass-bottom dishes (FluoroDish, FD3510, WPI) previously coated with 5 μg/ml fibronectin ...
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No products found
because this supplier's products are not listed.
Hana Saoud, et al.,
bioRxiv - Neuroscience 2021
Quote:
... It was possible to identify neonates by means of their three-digit metal ear tags (ref. 52-4716, Harvard Apparatus, Les Ulis, France). After surgery ...
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No products found
because this supplier's products are not listed.
Natsuki Shichinohe, et al.,
bioRxiv - Biochemistry 2022
Quote:
Human bone ALP was purchased from CALZYME (San Luis Obispo, CA). Human CD73 His-tag was purchased from BPS Bioscience (San Diego ...
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No products found
because this supplier's products are not listed.
Anna Ilaria Przytula-Mally, et al.,
bioRxiv - Biophysics 2023
Quote:
... for the human CPEB3 complex and PEG/Ion screen (Hampton Research) for the chimpanzee complex ...
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No products found
because this supplier's products are not listed.
David A. Lomas, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Crystallisation of recombinant Cys232Ser α1-antitrypsin was carried out in 2-well MRC crystallisation plates with a Mosquito robot (TTP Labtech) using 100nl protein solution and 100nl well solution ...
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No products found
because this supplier's products are not listed.
Hongbing Zhang, et al.,
bioRxiv - Bioengineering 2020
Quote:
The E-ALPHA® human scFv antibody phage display libraries (Eureka Therapeutics) were used for the selection of human antibody constructs specific to SARS-CoV-2 spike protein ...
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No products found
because this supplier's products are not listed.
S. Karkampouna, et al.,
bioRxiv - Cell Biology 2021
Quote:
Mouse and human liver tissues were homogenised with UltraTurrax homogenizer (T25 basic, IKA) and TRIpure reagent (Roche) ...
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No products found
because this supplier's products are not listed.
Thamiya Vasanthakumar, et al.,
bioRxiv - Biochemistry 2021
Quote:
Yeast strain SABY31 (VMA1-3×FLAG, stv1Δ)25 in YPD media (11 L) was grown in a Microferm fermenter (New Brunswick Scientific) for 36 hours (OD660=6 to 9 ...
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No products found
because this supplier's products are not listed.
Tomoyuki Ohno, et al.,
bioRxiv - Bioengineering 2022
Quote:
... 201B7 human iPS (RIKEN BRC, HPS0063) cells were maintained in StemFit AK02N medium (ReproCELL) in dishes coated with iMatrix-511 (Nippi) ...
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No products found
because this supplier's products are not listed.
Vincent Mouilleau, et al.,
bioRxiv - Developmental Biology 2020
Quote:
Human SA001 embryonic stem cell (ESC) line (male, RRID: CVCL_B347) was obtained from Cellectis and used accordingly to the French current legislation (Agency of Biomedicine ...
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No products found
because this supplier's products are not listed.
Matthew J. Shannon, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... The assay was validated using a standard curve calibrated against human methylation controls (EpigenDx, USA Cat ...
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No products found
because this supplier's products are not listed.
Delphine Planas, et al.,
bioRxiv - Immunology 2021
Quote:
... 2) a CE-Marked ELISA assay for detection of IgG against the full-length recombinant N protein from Epitope Diagnostics (EDITM Novel coronavirus COVID-19 IgG). The test has a specificity of 96% and a sensitivity of 81% after 28 days POS in our hands ...
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No products found
because this supplier's products are not listed.
Stephen A. Schumacher, et al.,
bioRxiv - Physiology 2021
Quote:
Serum PTH concentrations were measured with a human-specific immunoradiometric assay (Scantibodies Laboratory, Santee, CA, USA) with a working range of 6.5-2328 pg/mL ...
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No products found
because this supplier's products are not listed.
Benjamin D. Hobson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... A small hole was drilled into the skull and 400 nL of 0.9% saline or 0.9% saline + 100 ng/μL recombinant mouse IFN-γ (40 ng IFN-γ total) was injected through a pulled glass pipet using a Nanoject 2000 (Drummond Scientific; 8 pulses of 50 nL). At 5 minutes after injection ...
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No products found
because this supplier's products are not listed.
Xin Wang, et al.,
bioRxiv - Immunology 2022
Quote:
Mouse trachea and human ALI culture samples were harvested and fixed with 4% PFA (BM-155, Boston BioProducts) overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Michal Schwartz, et al.,
bioRxiv - Microbiology 2022
Quote:
... using FAM labeled HCMV primer and probe: Human CMV HHV5 kit for qPCR using a glycoprotein B target (PrimerDesign); and HEX labeled RPP30 copy number assay for ddPCR (Bio-Rad) ...
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No products found
because this supplier's products are not listed.
Marco Losa, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... were dispensed at various volumes into human ASC-coated 1,536-well plates using contactless dispensing with an ECHO 555 Acoustic Dispenser (Labcyte). Thereby ...
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No products found
because this supplier's products are not listed.
Kyung-Jin Jang, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
Alpha Glutathione S-Transferase (α-GST): levels were quantified in human model effluent samples from the upper channel using an ELISA kit (DiaPharma). The assay was run following the vendor protocol using a standard curve ranging from 0-64 µg/L ...
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No products found
because this supplier's products are not listed.
Ram Sagar, et al.,
bioRxiv - Genetics 2023
Quote:
To acquire a high yield of functional neurons we transduced the generated human iPSCs with Ngn2 and rTTA expressing lentivirus (lentivirus was purchased by Cellomics Technology, Maryland USA). We followed an established protocol for generation of induced neuronal cells in 21 days 17 ...