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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Zhuo Chen, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Protein signal data were extracted from the multicolor fluorescent images using GenePix Pro 6.1 (Molecular Devices) by aligning a microtrough array template with feature blocks per antibody per microtrough to the protein signal features ...
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No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
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No products found
because this supplier's products are not listed.
Mathew Miller, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... RNAs encoding green fluorescent protein (GFP) were complexed with TransIT (Mirus Bio, Madison, WI) according to the manufacturer’s instructions by diluting in EMEM without serum or antibiotics and incubating for 2-5 minutes ...
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No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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No products found
because this supplier's products are not listed.
Joanna Young, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab) for generation of polyclonal antibodies ...
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Bimake 2x SYBR Green qPCR master mix performs qPCR with highest sensitivity, specIFicity and...
Cat# B21202, SKU# B21202-5 mL,
5 mL, $137.00
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Meng Zhao, et al.,
bioRxiv - Cell Biology 2022
Quote:
... primers and SYBR-green fluorescent dye (Bimake, Houston, TX, USA) were used ...
-
No products found
because this supplier's products are not listed.
Nishit Goradia, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Cells were rinsed with PBS before adding 20 μl PBS for the Green Fluorescent Protein fluorescence intensity measurement on an Infinite 200 Pro plate reader (TECAN, Switzerland), using fluorescence excitation at 465 nm and fluorescence emission at 535 nm ...
-
No products found
because this supplier's products are not listed.
Swann Floc’hlay, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Treatments with recombinant BMP2/4 (Recombinant Mouse BMP-4 Protein, CF 5020-BP/CF) or Nodal (Recombinant human Nodal protein 3218-ND-025/CF ...
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No products found
Daniel M. Sapozhnikov, Moshe Szyf,
bioRxiv - Molecular Biology 2020
Quote:
... and dCas9 recombinant protein (Applied Biological Materials Inc.). After 10 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
No products found
because this supplier's products are not listed.
Beatriz Gil, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Recombinant alpha- synuclein protein was a gift from rPeptide (GA, USA).
-
No products found
because this supplier's products are not listed.
Leonardo Augusto, et al.,
bioRxiv - Microbiology 2020
Quote:
... the membrane was incubated with Pierce enhanced chemiluminescence (ECL) immunoblot substrate to visualize the proteins levels using the FluorChem E system (Protein Simple).
-
No products found
because this supplier's products are not listed.
Callum J. Fraser, et al.,
bioRxiv - Microbiology 2022
Quote:
1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
-
No products found
because this supplier's products are not listed.
Wadie D. Mahauad-Fernandez, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Fluorescent oligonucleotides (Akoya Bioscience) were added to this reporter solution at a final concentration of 1:50 in a total of 250 µl per well ...
-
No products found
because this supplier's products are not listed.
Michael J. Lucas, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and fluorescent measurements were taken using a fluorescent plate reader (Clariostar, BMG Labtech) at 37 °C ...
-
No products found
because this supplier's products are not listed.
Cansu Yildirim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... recombinant mouse IFNγ (Immunotools, #12343537), recombinant mouse IL4 (Immunotools ...
-
No products found
because this supplier's products are not listed.
Shirelle X. Liu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... SYBR-green PCR (Fast SYBR green master mix, ABI) was used to amplify Gapdh and Myod1 ...
-
No products found
because this supplier's products are not listed.
Anisha D’Souza, et al.,
bioRxiv - Bioengineering 2021
Quote:
Reporter plasmid DNA constructs encoding the firefly luciferase gene (gWIZ-Luc/Luc- pDNA, 6732 bp) and enhanced green fluorescent protein (gWiz-GFP/GFP-pDNA, 5757 bp) were purchased from Aldevron (Fargo, ND). The stock solutions of pDNA were diluted in 10 mM Tris-HCl containing 1 mM EDTA (pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Julian Schöllkopf, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
N-terminally His-tagged Pasteurella multocida toxin (PMT) was coated at 10 µg/mL (500 ng/well ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
MEENAKSHI TETORYA, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The His-tagged peptides (GMA4CG_WT and GMA4CG_V6) phospholipid binding assays were performed using the PIP StripsTM (Echelon Biosciences Inc., CA) as previously described (Islam et al. ...
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No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ben R. Hopkins, Olga Barmina, Artyom Kopp,
bioRxiv - Developmental Biology 2022
Quote:
... legs expressing fluorescent proteins were mounted in Fluoromount 50 (SouthernBiotech). For immunohistochemistry ...
-
No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
-
No products found
because this supplier's products are not listed.
Mario K. Shammas, et al.,
bioRxiv - Cell Biology 2021
Quote:
... silver enhanced (HQ kit, Nanoprobes), en bloc mordanted with 0.25-0.5% uranyl acetate in acetate buffer (pH 5.0 ...
-
No products found
because this supplier's products are not listed.
Ayat Zawawi, et al.,
bioRxiv - Immunology 2019
Quote:
... the VLP recombinant proteins were separated on 10% polyacrylamide gels and stained with instant blue protein stain (Expedeon).
-
No products found
because this supplier's products are not listed.
Ella F.J. Halcrow, et al.,
bioRxiv - Cell Biology 2022
Quote:
... HeLa cells expressing the different GFP-tagged proteins were plated on an open μ-Slide with 8 wells (Ibidi, 80826) in complete medium containing 0.5 μM SiR-DNA dye (SpiroChrome) ...
-
No products found
because this supplier's products are not listed.
Johanna K. Kostka, Ileana L. Hanganu-Opatz,
bioRxiv - Neuroscience 2022
Quote:
... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
-
No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
-
No products found
because this supplier's products are not listed.
Robin Hoeven, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Farwa Sajadi, Jean-Paul Paluzzi,
bioRxiv - Physiology 2024
Quote:
... AedaeItp-l or control Egfp (enhanced green fluorescent protein) dsRNA using a Nanoject III Programmable Nanoliter Injector (Drummond Scientific, Broomall, PA, USA).
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Asako Haraguchi, et al.,
bioRxiv - Microbiology 2022
Quote:
... berghei (ANKA strain) that constitutively expresses green fluorescent protein (GFP) was maintained via serial passage in ICR mice (Charles River Laboratories Japan Inc. ...
-
Cat# HY-P2528,
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Min Feng, et al.,
bioRxiv - Developmental Biology 2023
Quote:
The green fluorescent dye 5(6)-Carboxyfluorescein diacetate succinimidyl ester (CFSE, MedChemExpress) was used to track the bacteria ...
-
No products found
because this supplier's products are not listed.
Kathryn L. Wofford, et al.,
bioRxiv - Bioengineering 2019
Quote:
... either human recombinant myelin basic protein (Alfa Aesar, cat. #AAJ66051LB0) fluorescently labeled with Vybrant DiD (Molecular Probes ...
-
No products found
because this supplier's products are not listed.
Arun Upadhyay, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 10 nM recombinant MT3 protein (Boster Bio Cat no. PROTP25713) was added at a in additional wells ...
-
No products found
because this supplier's products are not listed.
Senthil-Kumar Devan, et al.,
bioRxiv - Microbiology 2022
Quote:
... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
-
No products found
because this supplier's products are not listed.
Yoji Tsugawa, et al.,
bioRxiv - Microbiology 2024
Quote:
... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
-
No products found
because this supplier's products are not listed.
Raed Ibraheim, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... was coated with 0.5 μg of recombinant Nme2Cas9 protein suspended in 1× coating buffer (Bethyl E107). The plate was incubated for 12 h at 4°C with shaking ...
-
No products found
because this supplier's products are not listed.
Maëliss Champagne, et al.,
bioRxiv - Microbiology 2023
Quote:
... was incubated with 50µL of magnetic beads coated with recombinant protein (2 µg protein/1.25 × 106 beads) in 96-well flat-bottom chimney plates (Greiner bio one, Frickenhausen, Germany) on a plate shaker at 300 rpm for 16 h at 4 °C in the dark ...
-
No products found
because this supplier's products are not listed.
Lucia Gonzalo, et al.,
bioRxiv - Plant Biology 2021
Quote:
... 4°C and the supernatants were incubated overnight with 1/100 RNAPII or HIS antibodies (RNAP II AS11 1804, HIS AS20 4441, Agrisera) and 30 μl SureBeads (BioRad) ...
-
No products found
because this supplier's products are not listed.
Amos Zamir, et al.,
bioRxiv - Cell Biology 2020
Quote:
... fluorescent calcium dye (Calbryte 520, AAT Bioquest) was loaded for 40 minutes prior to imaging.
-
No products found
because this supplier's products are not listed.
Jing Zhang, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Human embryonic kidney (HEK) 293T and A549-vimentin (VIM)-red fluorescent protein (RFP) cell lines were originally purchased from American Type Culture Collection (ATCC). PaTu-S ...
-
No products found
because this supplier's products are not listed.
David Chen, et al.,
bioRxiv - Bioengineering 2020
Quote:
... we used a plastic fluorescent slide (Chroma Technologies 92001) and a slide with 4 um fluorescent beads (FocalCheck #1 ...
-
No products found
because this supplier's products are not listed.
Ashley V. Makela, et al.,
bioRxiv - Biophysics 2021
Quote:
... for fluorescent imaging and CoolSNAP DYNO (Photometrics, AZ, USA) for color and brightfield acquisition.
-
No products found
because this supplier's products are not listed.
Michał A. Surma, et al.,
bioRxiv - Biochemistry 2021
Quote:
... healthy diets differing by protein content: Teklad global 14% protein (Envigo #2014S, low protein) and Teklad global 18% protein (Envigo #2018S ...
-
No products found
because this supplier's products are not listed.
Wei-Siang Liau, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Protein identification was carried out using Protein Pilot 5.0 (SCIEX) for database searching.