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No products found
because this supplier's products are not listed.
Zeynep Tarcan, et al.,
bioRxiv - Biochemistry 2021
Quote:
Recombinant His-tagged ubiquitin and ubiquitin mutants were purchased from Boston Biochem, dissolved in LFB1/50 buffer (10% sucrose ...
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No products found
because this supplier's products are not listed.
Meisam Nosrati, et al.,
bioRxiv - Biochemistry 2019
Quote:
... His-tagged RmtC proteins were detected by immunoblotting with a rabbit anti-6×His antibody (α6×His; Proteintech; 10001-0-AP) overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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No products found
because this supplier's products are not listed.
Geoffrey L. Rogers, et al.,
bioRxiv - Immunology 2023
Quote:
... then stained with fluorescent anti-His antibody (Miltenyi Biotec), along with other antibodies according to panels described in Supplementary Table 6 ...
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No products found
because this supplier's products are not listed.
Priya Gatti, et al.,
bioRxiv - Cell Biology 2024
Quote:
... followed by fluorescent-tagged secondary antibodies (1:500; Jackson Immunoresearch). Cells were co-stained with Rhodamine-phalloidin (1:250 ...
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No products found
because this supplier's products are not listed.
Jason S Presnell, William E Browne,
bioRxiv - Developmental Biology 2021
Quote:
... Recombinant Cas9 protein (PNA Bio) and sgRNAs were injected at concentrations of 400 ng/µl of Cas9 protein and 100 ng/µl for each sgRNA ...
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No products found
because this supplier's products are not listed.
Maria Fernanda Fernandes, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and M protein (YP_009724393.1) tagged at the C-terminus with green fluorescent protein (Ex-NV225-M03) were from GeneCopoeia (Rockland, MD, U.S.A). The control plasmid was pCMV-3Tag-3A (pCMV ...
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No products found
because this supplier's products are not listed.
Pablo Alcón, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 20 µM His-tagged ubiquitin (Enzo Life Sciences) in a reaction buffer of 50 mM HEPES pH 7.5 ...
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The recombinant human MSLN is expressed as a 317-amino acid protein consisting of Glu296 -...
Cat# MSLN-001H,
100ug , USD $699
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Sunil Yeruva, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Recombinant protein of human DSG2 tagged with IgG Fc domain (Fc) (Creative Biomart; #DSG2-1601H) and N-CAD-Fc (Sino Biological ...
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No products found
because this supplier's products are not listed.
Bart Coppens, et al.,
bioRxiv - Microbiology 2022
Quote:
... green fluorescent aminated (radius = 50 nm) or green fluorescent carboxylated (radius = 60 nm) polystyrene nanoparticles (Spherotech) were gently pipetted directly below the liquid-air interface to avoid structural disturbance of the biofilms ...
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No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
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No products found
because this supplier's products are not listed.
Kieran M. Short, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... fluorescent tagged Dolichos Biflorus Agglutinin (DBA) (Vector Labs, cat# FL1031, 1:100 dilution), and nuclei with Hoechst-33342 (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Marco Cassani, et al.,
bioRxiv - Bioengineering 2023
Quote:
... green fluorescent protein (GFP)-positive cells were FACS-sorted (MoFlo Astrios, Beckman Coulter, California, USA) as single cells into a 96-well plate and clonally propagated ...
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No products found
because this supplier's products are not listed.
Nehanjali Dwivedi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The HIS-tagged protein was purified on Ni-NTA (cat. no. 6562; BioVision; abcam, Inc.) column ...
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No products found
because this supplier's products are not listed.
Yoshiaki Kamiyama, et al.,
bioRxiv - Plant Biology 2020
Quote:
... Both MBP-tagged and GST-tagged recombinant proteins were further purified with a Nanosep® 30-kDa size-exclusion column (PALL, NY). In addition ...
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No products found
because this supplier's products are not listed.
Oluwadamilola O. Lawal, et al.,
bioRxiv - Neuroscience 2024
Quote:
... chicken anti-green fluorescent protein (1:1000 [GFP-1020, Aves Labs, CA]), Guinea pig anti-VGAT (Synaptic Systems #131004) ...
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No products found
because this supplier's products are not listed.
Kasidit Rattanawong, et al.,
bioRxiv - Plant Biology 2023
Quote:
... egg cells and multicellular structures expressing green fluorescent protein (GFP) fusion proteins or stained with fluorescent probes were observed using an LSM 710 CLS microscope (Carl Zeiss, Jena, Germany), with excitation and emission wavelengths specific to each type of fluorophores as described below ...
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No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
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No products found
because this supplier's products are not listed.
Deniz B. Unal, Steven R. Caliari, Kyle J. Lampe,
bioRxiv - Bioengineering 2020
Quote:
Green fluorescent protein positive (GFP+) mosaic analysis with double markers (MADM) OPCs were expanded on T75 (Corning CellBind) treated tissue culture plates ...
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No products found
because this supplier's products are not listed.
Kevin W. Ng, et al.,
bioRxiv - Immunology 2020
Quote:
... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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No products found
because this supplier's products are not listed.
Timothy S. Strutzenberg, et al.,
bioRxiv - Biophysics 2019
Quote:
... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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No products found
because this supplier's products are not listed.
Maria E. Cilento, et al.,
bioRxiv - Microbiology 2023
Quote:
... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
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No products found
because this supplier's products are not listed.
Lilian M. Martinelli, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... as well as with the fluorescent DNA interlayer Eva Green (Biotium, CA, USA), in a total volume of 6μL ...
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No products found
because this supplier's products are not listed.
Luned M. Badder, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... Quantitative-RT PCR was then performed using the SensiFAST SYBR Green Hi-ROX master mix (Bioline). Primers were designed (Sigma Aldrich ...
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No products found
because this supplier's products are not listed.
Jacqueline Findlay, et al.,
bioRxiv - Microbiology 2019
Quote:
... Enhanced genome sequencing (combining Illumina and MinION reads) was performed on one transconjugant ...
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No products found
because this supplier's products are not listed.
Chien-Wei Wang, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Recombinant His-tagged streptavidin (Fitzgerald Industries, Acton, MA) at a concentration of 0.75 μM was used as a negative control molecule ...
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No products found
because this supplier's products are not listed.
Derin Sevenler, Mehmet Toner,
bioRxiv - Bioengineering 2023
Quote:
... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
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No products found
because this supplier's products are not listed.
Alexander W. Justin, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Green fluorescent protein (GFP) and Red Fluorescent Protein (RFP) human umbilical vein endothelial cells (HUVECs, Promocell), normal human lung fibroblasts (NHLFs ...
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No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
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No products found
because this supplier's products are not listed.
Soon Yi, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... into enhanced Piggybac (ePB) doxycycline-inducible expression vectors.46 Overlap PCR constructs encoding FLAG-APEX2 fusions with mKate2 fluorescent protein (Evrogen, cat. #FP181) were cloned between BamHI and NotI sites in ePB vectors as follows ...
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No products found
because this supplier's products are not listed.
Christian Heuss, et al.,
bioRxiv - Microbiology 2022
Quote:
... SYBR Green based Product Enhanced Reverse Transcriptase assay (SG-PERT) was performed using the Takyon SYBR green kit (Eurogentec) to quantify HCVpp titers used for infection (74).
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No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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No products found
because this supplier's products are not listed.
Robert Bucki, et al.,
bioRxiv - Biophysics 2022
Quote:
... Recombinant human protein arginine deiminase 4 (PAD4) was from Cayman Chemical, Inc.
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No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
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No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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No products found
because this supplier's products are not listed.
Shravanthi Rajasekar, et al.,
bioRxiv - Bioengineering 2021
Quote:
Green Fluorescent Protein-tagged Human Umbilical Vein Endothelial Cells (GFP-HUVECs, Angio-Proteomie, Cat# CAP-0001GFP) were grown in Endothelial Cell Growth Media (ECGM2 ...
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No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
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No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
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No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
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No products found
because this supplier's products are not listed.
Haimin Song, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Stable C6 cells expressing green fluorescent protein (GFP)were selected with 10μM Puromycin (Solarbio, P8230, 5 μg/ml).
-
No products found
because this supplier's products are not listed.
Kevin D. Whitley, et al.,
bioRxiv - Microbiology 2023
Quote:
... The green fluorescent D-amino acid BADA32 (Tocris Bioscience) was added to a final concentration of 0.5 mM and tubes were incubated at 30°C with shaking for 90 min ...
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No products found
because this supplier's products are not listed.
Yann Aquino, et al.,
bioRxiv - Genomics 2022
Quote:
... Recombinant IFN-χ protein (PBL Assay Science) was used as a calibrator ...
-
No products found
because this supplier's products are not listed.
Kanak Raina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Braulio Cardenas-Benitez, et al.,
bioRxiv - Biophysics 2023
Quote:
... and the media further supplemented with 30 U mL-1 of human recombinant IL-2 protein (STEMCELL Technologies, USA). T cells were incubated in a humidified incubator with 5% CO2 at 37°C and were split to 0.5×106 cells mL-1 when their concentration surpassed 2.5×106 cells per well or when medium became depleted (turning yellow in color) ...