-
No products found
because this supplier's products are not listed.
Vera F. Monteiro-Cardoso, et al.,
bioRxiv - Cell Biology 2022
Quote:
... counterstained with uranyl acetate and observed under a MET JEOL 1400 equipped with a Orius High speed (Gatan) camera.
-
No products found
because this supplier's products are not listed.
Matthew J. Stevenson, et al.,
bioRxiv - Cancer Biology 2022
Quote:
DNA sequences flanked by XhoI and NdeI restriction sites and encoding for N-terminal GST-tagged-SIX1 or N-terminal GST-tagged-SIX1-Q177R homeodomains were synthesized by Integrative DNA Technologies (IDT) as gBlocks (Supplemental Table 2) ...
-
No products found
because this supplier's products are not listed.
Glenda Comai, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... The Met inhibitors used were MGCD-265 (10μM, Selleck, 50mM stock in DMSO) and PF-0417903 (10-20μM, AbMole, 26.8mM stock in DMSO).
-
No products found
because this supplier's products are not listed.
Shiri Levy, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... 25 ng/ml human recombinant FGF4 (Reliatech), 2 ng/ml human recombinant TGF-ß1 (PeproTech) ...
-
No products found
because this supplier's products are not listed.
Kanae Tsubotani, et al.,
bioRxiv - Biochemistry 2021
Quote:
... FITC-labelled lysozyme (LS1-FC-1, Nanocs Inc., USA) instead of lysozyme was mixed with ovalbumin in 5mM Tris buffer (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
Imke M.E. Schuurmans, et al.,
bioRxiv - Neuroscience 2024
Quote:
... RevitaCell™ and 2.5% AM FCS (ScienCell®; 0010). Then medium of the neurons was refreshed with neurobasal medium supplemented with B-27 ...
-
No products found
because this supplier's products are not listed.
Cassian Afting, et al.,
bioRxiv - Bioengineering 2024
Quote:
WNT surrogate-Fc Fusion Protein (Wnt-surrogate; ImmunoPrecise Antibodies Ltd. ...
-
No products found
because this supplier's products are not listed.
Musleh M. Muthana, et al.,
bioRxiv - Immunology 2023
Quote:
... Azide-free human IgG-Fc was purchased from Athens Research and Technology (Athens ...
-
No products found
because this supplier's products are not listed.
Scott Birks, et al.,
bioRxiv - Bioengineering 2023
Quote:
... prior to being tagged with a rabbit anti-nesprin2 antibody (1:300; ImmuQuest IQ565). After primary antibody tagging ...
-
No products found
because this supplier's products are not listed.
Michael S. Balzer, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... and 10 U/ml recombinant mouse interferon-□ (Cell Sciences, Canton, MA) at 33 °C (permissive conditions ...
-
No products found
because this supplier's products are not listed.
Elena Arutyunova, et al.,
bioRxiv - Biophysics 2021
Quote:
... The fusion protein was subsequently digested with His-tagged SUMO protease (McLab, South San Francisco, CA) at 4 °C for 1–2 h to remove the SUMO tag and the resulting cleavage mixture was then passed through Ni-NTA resin column ...
-
No products found
because this supplier's products are not listed.
Melisa Lázaro, et al.,
bioRxiv - Microbiology 2020
Quote:
... This was confirmed by labeling N-terminally His6-tagged mL-GDH180 with Ni-NTA-Nanogold (Nanoprobes) and visualizing particles by negative staining electron microscopy ...
-
No products found
because this supplier's products are not listed.
Uri Elia, et al.,
bioRxiv - Immunology 2020
Quote:
... pseudouridine-substituted Fc-conjugated RBD mRNA (331-524 aa) was purchased from TriLink Bio Technologies (San Diego ...
-
No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
-
No products found
because this supplier's products are not listed.
Wiktoria Ogrodzińska, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The recombinant plasmids were isolated using plasmid purification kit (Bio Basic Canada) and the inserts were verified by sequencing (Genomed ...
-
No products found
because this supplier's products are not listed.
Chungwon Park, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Neurons were pre-incubated with 3 nM of CypHer5E-tagged anti-VGAT antibodies (131 103CpH, Synaptic Systems, Germany) in the culture medium and incubated in the 5% CO2 incubator for 2 h at 37°C to specifically label inhibitory synaptic vesicles in presynaptic terminals ...
-
No products found
because this supplier's products are not listed.
Yijiun Zhang, Joachim Seemann,
bioRxiv - Cell Biology 2023
Quote:
... Beads were washed with lysis buffer and Flag-tagged GRASP55 and GRASP65 were eluted with 0.5 mg/ml 3xFLAG peptide (Apexbio) in lysis buffer ...
-
No products found
because this supplier's products are not listed.
Darya V. Voronina, et al.,
bioRxiv - Immunology 2022
Quote:
... [48] fused with human IgG1 Fc-fragment and Llama IgG2b hinge were purchased from Evrogen (Russia). CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio ...
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No products found
because this supplier's products are not listed.
Koichi Sato, Aiko G.M. Hendrikx, Puck Knipscheer,
bioRxiv - Biochemistry 2023
Quote:
... The wild-type protein was overexpressed as a N-terminal His6-tagged protein in the E.coli JM109(DE3) cells (Intact Genomics) cultured in 4.4 L LB medium and purified by the previously described method53 ...
-
No products found
because this supplier's products are not listed.
Colin Lock, Melissa M. Gabriel, Bastian Bentlage,
bioRxiv - Molecular Biology 2023
Quote:
DNA was extracted from each tagged colony using the GenCatch genomic DNA extraction kit (Epoch Life Science, Sugar Land, TX) following the manufacturer’s protocol for tissue samples ...
-
No products found
because this supplier's products are not listed.
Goncalo Fernandes, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Injections and recombinant selection based on DsRed expression in the eye were performed by BestGene. For transformants ...
-
No products found
because this supplier's products are not listed.
Sameh S. M. Soliman, et al.,
bioRxiv - Microbiology 2021
Quote:
Rabbit and mouse monoclonal antibodies against recombinant mucoricin coupled to KLH were raised by ProMab Biotechnologies Inc.11 ...
-
No products found
because this supplier's products are not listed.
Asano Watanabe, et al.,
bioRxiv - Cell Biology 2021
Quote:
... GFP-tagged human vWF plasmid (Romani de Wit et al., 2003) was kindly provided by Jan Voorberg (Stichting Sanquin Bloedvoorziening, Netherland) under Materials Transfer Agreement ...
-
No products found
because this supplier's products are not listed.
M. Dolores Martin-de-Saavedra, et al.,
bioRxiv - Neuroscience 2019
Quote:
... CNTNAP2-Fc secreted in the medium was affinity purified using ProteinA-CaptivA TM PriMAB (RepliGen, Waltham, MA) and subsequently cleaved with recombinant 3C protease to remove the Fc fragment and leave the mature (i.e. ...
-
No products found
because this supplier's products are not listed.
Lianghui Zhang, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... and levels of the IgG1 Fc moiety were measured by Human IgG ELISA Kit (Immunology Consultants Laboratory). To characterize different routes side-by-side ...
-
No products found
because this supplier's products are not listed.
Jack A. Bryant, et al.,
bioRxiv - Microbiology 2020
Quote:
Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
-
No products found
because this supplier's products are not listed.
Young Sun Hwang, M. Andrés Blanco, Kotaro Sasaki,
bioRxiv - Developmental Biology 2022
Quote:
... hiPSCs were cultured on plates coated with recombinant laminin-511 E8 (iMatrix-511 Silk, Nacalai USA) and maintained under feeder-free conditions in StemFit® Basic04 medium (Ajinomoto ...
-
No products found
because this supplier's products are not listed.
Khairunnisa Mentari Semesta, et al.,
bioRxiv - Cell Biology 2020
Quote:
sgRNA transduced cells seeded on 6-well plates at ~60% confluency were transfected with 2 μg flag-tagged β2-AR for 48 hr using JetPrime Transfection reagent (Genesee, Cat #55134) following manufacturer’s protocols ...
-
No products found
because this supplier's products are not listed.
Shuai-dong Chen, et al.,
bioRxiv - Immunology 2023
Quote:
... the morphology of PLGA/FC ECM scaffold was observed by scanning electron microscopy (SEM; JSM-7500F, JEOL, Japan). Afterwards ...
-
No products found
because this supplier's products are not listed.
Henriette Hoffmann-Veltung, et al.,
bioRxiv - Immunology 2022
Quote:
... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
-
No products found
because this supplier's products are not listed.
Mariano Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
The full-length PfS1 recombinant purified protein was used to immunized rabbits subcutaneously following a two-months standard procedure (Proteogenix, France). Briefly ...
-
No products found
because this supplier's products are not listed.
Eli Fritz McDonald, et al.,
bioRxiv - Cell Biology 2021
Quote:
... purified from B lymphocyte hybridoma cells using a Recombinant Protein G - Sepharose 4B Affinity Column on an ÄKTA start protein purification system (GE Life Science Product # 29022094). Antibodies were used in immunoblotting buffer (5% bovine serum albumin (BSA ...
-
No products found
because this supplier's products are not listed.
Stacia M. Nicholson, Francis A.X. Schanne,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
Recombinant mouse TNFSF11 (IBI Scientific, Indiana), also known as RANKL ...
-
No products found
Lukas Kunz, et al.,
bioRxiv - Plant Biology 2022
Quote:
... for FLAG tagged effectors or WesternBright ECL HRP substrate (Advansta) for Pm8-HA.
-
No products found
because this supplier's products are not listed.
Faisal Almansour, et al.,
bioRxiv - Cell Biology 2024
Quote:
... including fluorescently tagged dUTP (Dyomics, DY488-dUTP: Cat. # 488-34), 1 mM β-mercaptoethanol (Bio-Rad ...
-
No products found
because this supplier's products are not listed.
Yannan Tian, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The supernatant was recovered and the released f-[35S]-Met was counted in 2 mL of Bio-Safe II Complete Counting Cocktail (Research Products International). The maximum releasable fMet (fMetMax ...
-
No products found
because this supplier's products are not listed.
Anil H. Kadam, et al.,
bioRxiv - Bioengineering 2022
Quote:
... recombinant hu TGF-β1(ProSci, Poway, CA), anti-TGF-β1 IgY-Biotin (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
-
No products found
because this supplier's products are not listed.
Bhaskar Saha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... All GST-tagged proteins were expressed in Escherichia coli SoluBL21 (Genlantis). GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01) ...
-
No products found
because this supplier's products are not listed.
Yi Huang, et al.,
bioRxiv - Bioengineering 2023
Quote:
Negatively stained samples for TEM were prepared by placing NP synthesized with 0-2000 μM of KM-met in MilliQ water on 400 mesh lacey carbon grids (Ted Pella, Redding, CA, USA) for 5 minutes ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Lindsey R. Conroy, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Recombinant PNGaseF Prime was obtained from Bulldog Bio, Inc ...
-
No products found
because this supplier's products are not listed.
Xiaoning Yang, et al.,
bioRxiv - Immunology 2023
Quote:
... C1q recombinant protein (A400) was purchased from Quidel and its secondary anti C1q/HRP (ab46191 ...
-
No products found
because this supplier's products are not listed.
Coralie Berthoux, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant human BDNF protein was purchased from Hello Bio. Salts for making ACSF and internal solutions were purchased from Sigma-Aldrich.
-
No products found
because this supplier's products are not listed.
Isaac R. Cinco, et al.,
bioRxiv - Immunology 2023
Quote:
... Goat anti-monkey IgG (Fc) HRP (Brookwood Biomedical, Jemison, AL) was diluted then added to each well and incubated for 1-1.5 hr ...
-
No products found
because this supplier's products are not listed.
Jinye Liang, et al.,
bioRxiv - Microbiology 2023
Quote:
... and imaged on a Li-COR Odyssey Fc scanner (Carver Center for Genomics ...
-
No products found
because this supplier's products are not listed.
Rebekah B. Stuart, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant proteins were produced in Escherichia coli BL21 (DE3) cells (Bioline) as described previously (LaCourse ...
-
No products found
because this supplier's products are not listed.
Sonja Giger, et al.,
bioRxiv - Bioengineering 2021
Quote:
... recombinant human epidermal growth factor (hEGF, 20 ng/ml, Chimerigen Laboratories, CHI-HF-210EGF), recombinant human platelet derived growth factor (hPDGF-AB ...
-
No products found
because this supplier's products are not listed.
Inga V. Hochheiser, et al.,
bioRxiv - Immunology 2021
Quote:
The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
-
No products found
because this supplier's products are not listed.
Alberto Domingo López-Muñoz, et al.,
bioRxiv - Microbiology 2023
Quote:
... alone or in combination with purified recombinant proteins were placed in the lower chamber of a 96-well ChemoTx System plate (Neuro Probe # 101-5) in RPMI 1640 1 % FBS ...