-
No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
No products found
because this supplier's products are not listed.
Nehanjali Dwivedi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... The HIS-tagged protein was purified on Ni-NTA (cat. no. 6562; BioVision; abcam, Inc.) column ...
-
No products found
because this supplier's products are not listed.
Amy Q. Wang, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and male Cynomolgus monkey plasma (BioIVT) using a Pierce Rapid Equilibration Dialysis (RED ...
-
No products found
because this supplier's products are not listed.
William J Dower, et al.,
bioRxiv - Immunology 2023
Quote:
... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
-
No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
-
No products found
because this supplier's products are not listed.
Michelle E. Poling, et al.,
bioRxiv - Physiology 2022
Quote:
... Recombinant GFP protein (Cat# MB-0752, Vector labs), diluted in S2R+ cell growth media (64ng/μl to 4 ng/μl) ...
-
No products found
because this supplier's products are not listed.
Sindelka Radek, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... size selected and tagged by Illumina sequencing adapters ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
-
No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
-
No products found
because this supplier's products are not listed.
Yann Aquino, et al.,
bioRxiv - Genomics 2022
Quote:
... Recombinant IFN-χ protein (PBL Assay Science) was used as a calibrator ...
-
No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Andrew G. Diamos, et al.,
bioRxiv - Immunology 2020
Quote:
... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
-
No products found
because this supplier's products are not listed.
Rebekah B. Stuart, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Recombinant proteins were produced in Escherichia coli BL21 (DE3) cells (Bioline) as described previously (LaCourse ...
-
No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
-
No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
-
No products found
because this supplier's products are not listed.
Timothy S. Strutzenberg, et al.,
bioRxiv - Biophysics 2019
Quote:
... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
-
No products found
because this supplier's products are not listed.
Coraline Chéneau, et al.,
bioRxiv - Immunology 2020
Quote:
... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
No products found
because this supplier's products are not listed.
Adrian Westhaus, et al.,
bioRxiv - Bioengineering 2022
Quote:
Cynomolgus and Rhesus macaque primary hepatocytes were purchased from Lonza (Cat# MKC118 and Cat# MKR103). Human hepatocytes were also purchased from Lonza (Cat# HUM181971 ...
-
No products found
because this supplier's products are not listed.
Beatriz Gil, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Recombinant alpha- synuclein protein was a gift from rPeptide (GA, USA).
-
No products found
because this supplier's products are not listed.
Robert Bucki, et al.,
bioRxiv - Biophysics 2022
Quote:
... Recombinant human protein arginine deiminase 4 (PAD4) was from Cayman Chemical, Inc.
-
No products found
because this supplier's products are not listed.
Joseph Atherton, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
-
No products found
because this supplier's products are not listed.
Yan-Xia Liu, et al.,
bioRxiv - Cell Biology 2021
Quote:
... YFP-tagged proteins was imaged at ∼15 frames per second (fps) using an IX83 inverted fluorescent microscopy (Olympus) equipped with a through-the-objective TIRF system ...
-
No products found
because this supplier's products are not listed.
Mathieu Ferrari, et al.,
bioRxiv - Immunology 2021
Quote:
... Bound Fc-tagged proteins were detected with anti-human HRP-conjugated secondary antibodies (Jackson ImmunoResearch – 109-035-088) at 1:3000 dilution in PBS 0.5% BSA ...
-
No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5 μg of His-tagged H2A:H2B or His-H3:Flag-H4 (Diagenode) and 2 μg of Flag-hSpt16 were added to 250 μl of binding buffer (50 mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
-
No products found
because this supplier's products are not listed.
Joanna Young, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab) for generation of polyclonal antibodies ...
-
No products found
because this supplier's products are not listed.
Swann Floc’hlay, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Treatments with recombinant BMP2/4 (Recombinant Mouse BMP-4 Protein, CF 5020-BP/CF) or Nodal (Recombinant human Nodal protein 3218-ND-025/CF ...
-
No products found
because this supplier's products are not listed.
Robert W. P. Glowacki, et al.,
bioRxiv - Microbiology 2019
Quote:
... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
No products found
because this supplier's products are not listed.
Hye-Young Lee, et al.,
bioRxiv - Plant Biology 2020
Quote:
Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
-
No products found
because this supplier's products are not listed.
Callum J. Fraser, et al.,
bioRxiv - Microbiology 2022
Quote:
1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
-
No products found
because this supplier's products are not listed.
Cansu Yildirim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... recombinant mouse IFNγ (Immunotools, #12343537), recombinant mouse IL4 (Immunotools ...
-
No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
-
No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Kaihuai Li, et al.,
bioRxiv - Microbiology 2020
Quote:
... HtsH2C-HA-His or HtsH3C-Myc-His proteins (100 ng) were incubated with CIAP (Solarbio, Beijing, China) at 28°C for 60 min ...
-
No products found
because this supplier's products are not listed.
Stephanie L. LeValley, et al.,
bioRxiv - Microbiology 2019
Quote:
... and incubated with recombinant protein (GenScript, Sigma, Tocris) of each analyte for 4 h at room temperature ...
-
No products found
because this supplier's products are not listed.
Laurens H. Lindenburg, et al.,
bioRxiv - Biochemistry 2020
Quote:
... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
-
No products found
because this supplier's products are not listed.
Ayat Zawawi, et al.,
bioRxiv - Immunology 2019
Quote:
... the VLP recombinant proteins were separated on 10% polyacrylamide gels and stained with instant blue protein stain (Expedeon).
-
No products found
because this supplier's products are not listed.
Krisztina Ötvös, et al.,
bioRxiv - Plant Biology 2019
Quote:
... The purified recombinant protein was used to immunize rabbits by a company (Eurogentec). From the immunserum a crude IgG fraction was isolated by ammonium sulfate precipitation then IgG was further purified on protein gel blots of the antigen ...
-
No products found
because this supplier's products are not listed.
Baira Godneeva, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
-
No products found
because this supplier's products are not listed.
Yang Xu, et al.,
bioRxiv - Plant Biology 2019
Quote:
... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
-
No products found
because this supplier's products are not listed.
Can Bora Yildiz, et al.,
bioRxiv - Molecular Biology 2023
Quote:
Cells were stimulated with 5 µg/mL of either the recombinant ephrinA5-Fc (#374-EA, Biotechne) or Fc protein (#109-1103, Rockland) as control ...