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No products found
because this supplier's products are not listed.
Madison A. DeWinter, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
... his-tagged extracellular domain of the growth hormone receptor (GHR, BioVision 7477-10) was immobilized on the Ni-NTA sensor tip by dipping the tips in a 2.5 μg/ml solution of GHR for 400 s ...
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No products found
because this supplier's products are not listed.
Meenakshi Basu Shrivastava, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 500 ng human recombinant His-tagged ubiquitin-conjugating enzyme (E2) Ube2d3 (from BIOMOL International, #U0880), in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Hristina Vasileva, et al.,
bioRxiv - Immunology 2023
Quote:
... the SC and V2 domains were cloned into the pET15b expression vector as dual His (N-terminal) and Myc (C-terminal) tagged recombinant constructs (Biomatik, USA). A duplicate set of expression constructs were also cloned into the pGEX-4T-1 expression vector as single-tagged GST (N-terminal ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Deepti Mudaliar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Recombinant Human CKII alpha prime polypeptide His Protein (NBP2-22736) was purchased from Novus Biologicals (Centennial, CO, USA). CK2 Substrate (synthetic peptide RRRADDSDDDDD ...
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No products found
because this supplier's products are not listed.
Andrew G. Diamos, et al.,
bioRxiv - Immunology 2020
Quote:
... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
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No products found
because this supplier's products are not listed.
Kiren Baines, Jon D. Lane,
bioRxiv - Cell Biology 2019
Quote:
... HRP-tagged secondary antibodies were from Jackson ImmunoResearch; fluorescent Alexa-tagged antibodies were from Molecular Probes.
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No products found
because this supplier's products are not listed.
Cansu Yildirim, et al.,
bioRxiv - Neuroscience 2023
Quote:
... recombinant mouse IFNγ (Immunotools, #12343537), recombinant mouse IL4 (Immunotools ...
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No products found
because this supplier's products are not listed.
Joseph Atherton, et al.,
bioRxiv - Biochemistry 2020
Quote:
... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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No products found
because this supplier's products are not listed.
Ivan Martinez-Valbuena, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Recombinant α-synuclein (rPeptide) was thawed from −80 ◦C storage ...
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No products found
because this supplier's products are not listed.
Tomohisa Sebastian Tanabe, et al.,
bioRxiv - Biochemistry 2023
Quote:
... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
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No products found
because this supplier's products are not listed.
Adrian Westhaus, et al.,
bioRxiv - Bioengineering 2022
Quote:
Cynomolgus and Rhesus macaque primary hepatocytes were purchased from Lonza (Cat# MKC118 and Cat# MKR103). Human hepatocytes were also purchased from Lonza (Cat# HUM181971 ...
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No products found
because this supplier's products are not listed.
Kylie M. Konrath, et al.,
bioRxiv - Immunology 2021
Quote:
... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ...
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No products found
because this supplier's products are not listed.
Trung The Tran, et al.,
bioRxiv - Immunology 2022
Quote:
... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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No products found
because this supplier's products are not listed.
Ling He, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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No products found
because this supplier's products are not listed.
Teddy Kamata, Chun-Song Yang, Bryce M. Paschal,
bioRxiv - Biochemistry 2021
Quote:
... recombinant GST-tagged tandem AF1521 was fluorescently labeled using IRDye® 800CW Protein Labeling Kit (LI-COR Biosciences, Lincoln, NE, USA). Prepared samples were run on SDS-PAGE ...
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No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
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No products found
because this supplier's products are not listed.
A-M C Overstreet, et al.,
bioRxiv - Microbiology 2023
Quote:
... coli were made from an overnight agar culture and incubated with 3 mM HMGB1 recombinant protein containing a HIS tag (Abclonal) or an hFc tag (Sino Biological ...
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No products found
because this supplier's products are not listed.
Saurabh Srivastava, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
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No products found
because this supplier's products are not listed.
Gongshi Bai, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... FAM-tagged TelG probe (PNA Bio F1005) was diluted (1:100 ...
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No products found
because this supplier's products are not listed.
Sarah N. Zvornicanin, et al.,
bioRxiv - Biochemistry 2023
Quote:
HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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No products found
because this supplier's products are not listed.
Maria Steene Eriksen, et al.,
bioRxiv - Biochemistry 2019
Quote:
Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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No products found
because this supplier's products are not listed.
Yann Aquino, et al.,
bioRxiv - Genomics 2022
Quote:
... Recombinant IFNα17/αI (PBL Assay Science) was used as calibrator ...
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No products found
because this supplier's products are not listed.
Tridib Ganguly, et al.,
bioRxiv - Microbiology 2022
Quote:
... Recombinant His-tagged was ZccR overexpressed by addition of 0.2 mM isopropyl-β-D-1- thiogalactopyranoside (IPTG) (Teknova) and the culture was incubated for additional 3 h ...
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No products found
because this supplier's products are not listed.
Priyabrata Mohanty, et al.,
bioRxiv - Microbiology 2021
Quote:
... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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No products found
because this supplier's products are not listed.
Joanna Young, et al.,
bioRxiv - Microbiology 2020
Quote:
... Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab) for generation of polyclonal antibodies ...
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No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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No products found
because this supplier's products are not listed.
Alanna B. Chan, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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No products found
because this supplier's products are not listed.
Chia-Yang Chen, et al.,
bioRxiv - Plant Biology 2024
Quote:
... recombinant mononucleosomes (EpiCypher) as substrates ...
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No products found
because this supplier's products are not listed.
Yen-Der Li, et al.,
bioRxiv - Biochemistry 2023
Quote:
... transfected with 9 μg HA-tagged and 9 μg Flag-tagged constructs using TransIT-LT1 transfection reagents (Mirus). The transfected cells were cultured for another two days ...
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No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
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No products found
because this supplier's products are not listed.
Aleksei Kuznetsov, et al.,
bioRxiv - Biochemistry 2020
Quote:
Human recombinant ACE2-His protein (Icosagen OÜ, Estonia, cat# P-302-100) and SARS-CoV-2 Spike protein S1 (Icosagen OÜ ...
-
No products found
because this supplier's products are not listed.
Liam Hudson, et al.,
bioRxiv - Biochemistry 2023
Quote:
... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
-
No products found
because this supplier's products are not listed.
Cecilia Colombero, et al.,
bioRxiv - Cancer Biology 2021
Quote:
MDA-MB-231 cells transfected with GFP-tagged TKS5 and mCherry-tagged μ-adaptin were plated in CM or EBSS on glass bottom dishes (Ibidi Corporation) layered with unlabeled cross-linked gelatin as previously described ...
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No products found
Katharina M. Eyme, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
-
No products found
because this supplier's products are not listed.
Weisong Zhao, et al.,
bioRxiv - Biophysics 2022
Quote:
... equipped with an objective (100×/1.7 HI oil, APON, Olympus)and a sCMOS (Flash 4.0 V3 ...
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No products found
because this supplier's products are not listed.
Pia Hempelmann, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged protein was captured using NiNTA agarose beads (Cube Biotech) and eluted in 10 x 1 mL fractions with elution buffer containing 250 mM imidazole ...
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No products found
because this supplier's products are not listed.
Shuangshuo Jia, et al.,
bioRxiv - Cell Biology 2023
Quote:
... recombinant His-S ANXA1 protein (G15574, LifeSpan BioSciences, Seattle, WA, USA) was injected into the intra-articular cavity of SD rats four times weekly (1.3μg/50μL ...
-
No products found
because this supplier's products are not listed.
Huilei Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... An adenovirus used to overexpress C-terminal His-tagged LOXL2 was purchased from Vector Biolabs. Vitamin E (α-Tocopherol ...
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Recombinant Antigen
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Kei Haga, et al.,
bioRxiv - Microbiology 2021
Quote:
... and recombinant SARS-CoV S1 subunits-His tag (The Native Antigen Company, UK) diluted in phosphate-buffered saline (PBS ...
-
No products found
because this supplier's products are not listed.
Kanak Raina, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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No products found
because this supplier's products are not listed.
Ao Dong, et al.,
bioRxiv - Neuroscience 2020
Quote:
... His (Tocris), Epi (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Máté Fischer, et al.,
bioRxiv - Bioengineering 2019
Quote:
... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
-
No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
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No products found
because this supplier's products are not listed.
Abigail J. Courtney, et al.,
bioRxiv - Genetics 2020
Quote:
... and GFP-tagged H2A.Z (GFP; Rockland 600-301-215) 1 µl of the relevant antibody was used ...
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No products found
because this supplier's products are not listed.
Natalia Sanchez de Groot, et al.,
bioRxiv - Systems Biology 2019
Quote:
... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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No products found
because this supplier's products are not listed.
David M. Zuckerman, et al.,
bioRxiv - Microbiology 2022
Quote:
... The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD, Cocalico, Reamstown, PA; His-PilQOlut, Eurogentec, Seraing, BE). Sera were tested for cross-reactivity by immunoblotting lysates from wildtype M ...